28 results about "Salmonella enterica" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to clostridium butyricum and application thereof, and the preservation number of the clostridium butyricum is CGMCC (China General Microbiological Culture Collection Center) No.27853. According to the present invention, with the application of the bacterial strain, the species diversity of the host intestinal flora can be significantly reduced, the Firmicite / Bacteroidetes ratio can be significantly increased, the abundance of harmful bacteria such as Escherichia coli and Salmonella bacteria can be reduced, and the abundance of beneficial bacteria can be improved; in addition, the yield of butyric acid of the clostridium butyricum is larger than or equal to 1.5 g / L, and the activity of amylase in fermentation liquor of the clostridium butyricum is 16.04 + / -0.46 U / mL. When the feed additive is used in feed, intestinal functions of organisms can be effectively improved.

The present invention relates to the modification of an attenuated live strain of Salmonella enterica serovar Typhi, wherein its native surface-exposed polysaccharide and flagellin antigens can be converted to, or enhanced by, those of other strains of Salmonella, including Salmonella enterica serovar Paratyphi, Salmonella enterica serovar Typhimurium, and Salmonella enterica serovar Enteritidis. The present invention also relates to a modified strain of Salmonella enterica serovar Typhi suitable for use as a component of a vaccine against typhoid enteric fever and salmonellosis.

The present invention relates to immunogenic compositions comprising antigens derived from Salmonella enterica serotype tiphimurium (S. Typhimurium), Salmonella enterica serotype enteritidis (S. Enteritidis), and Salmonella enterica serotype typhi (S. Typhi). The present invention further relates to methods and compositions comprising GMMA for boosting the immune response to the S. Typhi antigen, vaccines and methods comprising immunogenic compositions, and the use of immunogenic compositions.

The invention discloses a broad-spectrum antibacterial lactobacillus plantarum, and belongs to the technical field of microorganisms. The lactobacillus plantarum 9-6 is separated from animal feed, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32568, the lactobacillus plantarum 9-6 and fermentation liquor thereof have an obvious inhibition effect on common vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi and vibrio vulnificus, and the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the advantages that the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the obvious inhibition effect; and in addition, the antibacterial agent has a relatively good antibacterial effect on common staphylococcus aureus, escherichia coli, listeria monocytogenes, salmonella enterica and bacillus cereus. The lactobacillus plantarum 9-6 has probiotic safety, can grow under an initial pH condition of 4-10 and a high-salt environment, and plays a role in bacteriostasis under an acidic condition. The invention lays a foundation for developing a good antibacterial probiotic preparation and application of a fermentation culture thereof.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The present invention relates to a bacteriocin (salmonin) for controlling Salmonella enterica. The bacteriocin is derived from Salmonella enterica. The salmonin can be expressed in plants and can be used in a method for preventing or reducing Salmonella enterica infection or contamination of a target.

The present invention relates to a novel bacteriophage OPT-SALO1 with specific killing ability for Salmonella enterica, an antibiotic composition comprising the bacteriophage, a composition for adding to a feed, a feed, a disinfectant or a cleaning agent, and a method for preventing or treating infectious diseases caused by Salmonella enterica comprising a step of administering the bacteriophage to a subject.

Technologies for the prevention and / or treatment of Salmonella infections.

The invention provides a primer probe combination, a kit, a nucleic acid detection method and application, and relates to the technical field of biochemical detection. The primer probe combination comprises a first primer pair and a first probe, wherein the first primer pair and the first probe are used for specifically amplifying pseudomonas aeruginosa; the second primer pair and the second probe are used for specifically amplifying enterococcus; the third primer pair and the third probe are used for specifically amplifying staphylococcus aureus; the fourth primer pair and the fourth probe are used for specifically amplifying the salmonella enterica. According to the primer and probe combination, a specific nucleotide sequence is utilized, efficient and accurate recognition of pseudomonas aeruginosa, enterococcus, staphylococcus aureus and salmonella enterococcus is achieved in a complex intestinal environment, and non-specific interference is effectively avoided; meanwhile, synchronous quantitative detection of four pathogens is supported, the detection efficiency is remarkably improved, and an accurate and sensitive detection means is provided for rapid clinical evaluation of flora conditions.

The present invention discloses an indirect ELISA kit for specifically detecting Salmonella enterica serovar Indiana antibodies and its application. The kit includes an antigen-coated enzyme-linked immunosorbent assay (ELISA) plate, enzyme-labeled antibody, negative serum, positive serum, concentrated washing solution, chromogenic solution, and reaction termination solution. Compared with the traditional slide agglutination antigen detection method, the kit of the present invention not only greatly improves the detection throughput, but also has good sensitivity, specificity, and repeatability.

The present invention relates to identifying and engineering a tail spike protein gene from a genome of bacteriophage SFP10 infected with a Salmonella strain, purifying and producing a recombinant protein using mSFP10TSP, which is a recombinant protein formulation for detecting Salmonella enterica, EGFP-mSFP10TSP fused with a green fluorescent protein, and SiBD-mSFP10TSP fused with an SiBD, and thereby specifically detecting the Salmonella strain.

The present invention relates to an immunogenic composition comprising antigens from Salmonella enterica mouse typhoid serotype (Salmonella typhoid), Salmonella enterica enteritis serotype (Salmonella enteritidis), and Salmonella enterica typhoid serotype (Salmonella typhoid). The invention also relates to methods and uses of compositions comprising GMMA for enhancing an immune response against Salmonella typhi antigens, vaccines comprising said immunogenic compositions, and methods and uses of said immunogenic compositions.

The invention discloses application of salinomycin to prevention and treatment of pathogenic microorganisms. According to the present invention, the compound provides significant inhibition activity for agricultural fungi such as Sclerotinia sclerotiorum, Botrytis cinerea, Pyricaria oryzae, Moniliniafacticola ACCC 36263, food-borne bacteria such as Escherichia coli O157: H7ATCC 35150, Bacillus cereus BN CC 103930, Listeria monocytogenes ATCC 19115 and Salmonella enteritidis ATCC 14028, and further provides a certain inhibition effect on other agricultural pathogenic bacteria, such that the compound is expected to be developed into the one type of the compound,

The present invention relates to immunogenic compositions comprising Salmonella enterica paratyphoid A serotype outer membrane vesicles (OMV), vaccines comprising said immunogenic compositions, and methods and uses of said immunogenic compositions. The invention also relates to Salmonella paratyphi A bacteria comprising the modified lipid A.

Disclosed are engineered outer membrane proteins (OMPs) that comprise one or more extracellular loops from the Salmonella enterica serovar Typhimurium protein PagC or a functional portion thereof. Methods of expressing the engineered outer membrane proteins in a cell, such as a Gram-negative bacteria, to increase the production of outer membrane vesicles are also provided.

To provide a novel treatment method for benign nervous system tumors, including schwannomas; and to provide a composition for use in the treatment method.SOLUTION: Provided herein are methods for a treating a subject having or at risk of having a benign nervous system tumor. The methods include administering to the subject a therapeutically effective amount of a composition comprising live attenuated Salmonella bacteria, optionally in combination with an immune checkpoint inhibitor and / or angiogenesis inhibitor. Preferably, the attenuated Salmonella is an attenuated strain of S. typhimurium, e.g., Salmonella enterica serovar typhimurium strain VNP20009 with modified lipid A (msbB-) and purine auxotrophic mutation (purI-).SELECTED DRAWING: None

The invention discloses a yeast microcapsule encapsulated modified drug-loaded silicone grease body as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method of the yeast microcapsule encapsulated modified drug-loaded silicone grease comprises the following steps: mixing disulfide bond modified dendritic mesoporous silica, an antibacterial drug, an anti-inflammatory drug and water to prepare drug-loaded dendritic mesoporous silica; the preparation method comprises the following steps: preparing drug-loaded dendritic mesoporous silica, coating the surface of the drug-loaded dendritic mesoporous silica with attenuated salmonella outer membrane vesicles, modifying the surface of the drug-loaded dendritic mesoporous silica with polyethyleneimine and hyaluronic acid, and finally coating with yeast microcapsules to obtain the modified drug-loaded silicone grease encapsulated by the yeast microcapsules. The modified drug-loaded silicone grease can achieve antibacterial, anti-inflammatory and intestinal flora recovery, provides an alternative scheme for traditional antibiotic treatment, and effectively solves the problems that the antibiotic treatment effect on animal salmonella enterica infection is poor and the like.

The present invention relates to an antibacterial composition comprising, as an active ingredient, a plantaricin peptide derived from a Lactiplantibacillus plantarum KM2 strain, wherein the plantaricin peptide derived from the strain and a plantaricin peptide combination were found to have antimicrobial activity against at least one selected from the group consisting of Flavobacterium sp, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, and acromobacter xylosoxidans (Alcaligenese xylosoxidans), Salmonella enterica, and Vibrio parahaemolyticus, and the plantaricin peptide and the plantaricin peptide combination were found to have antibacterial activity and a cell wall lysis effect against the Flavobacterium sp. strain, and accordingly, the plantaricin peptide derived from the Lactiplantibacillus plantarum KM2 strain and the plantaricin peptide combination are provided as antimicrobial agents.

The invention belongs to the technical field of biology, and provides a salmonella typhimurium endotoxin specific binding polypeptide and application thereof. The amino acid sequence of the polypeptide is selected from any one of SEQ ID No.1 to SEQ ID No.5 in a sequence list. Experiments prove that the polypeptide can be specifically bound with the salmonella typhimurium endotoxin, and compared with an existing anti-endotoxin clinical drug polymyxin B, the polypeptide has obviously higher specific binding capacity with the salmonella typhimurium endotoxin and also has obviously better salmonella typhimurium endotoxin detoxification capacity. On the basis, the invention also provides application of the polypeptide in removal, separation or analysis of salmonella typhimurium endotoxin and preparation of salmonella typhimurium endotoxin detoxification drugs. The polypeptide can be applied to the biomedical fields of biological separation, biological detection, disease diagnosis and treatment and the like.

The invention discloses a swine salmonella primer composition, a kit and application, the primer composition comprises a wzy154 upstream primer, a wzy154 downstream primer, a donor probe and a receptor probe, and further comprises an ST typing primer capable of amplifying aroC, dnaN, purE, hemD, thrA, hisD and sucA. The primer composition can be used for identifying the serotype of the swine salmonella, and compared with a slide agglutination test, the primer composition is simple and convenient to operate, short in time consumption and low in requirements on experience and operation level of experimenters, and has remarkable cost and flux advantages. The ST typing primer is further integrated by optimizing the primer composition, and PCR independent amplification of seven housekeeping genes is optimized into five reactions, so that the typing efficiency is remarkably improved, and epidemiological monitoring and evolution research are facilitated.

The present invention discloses an HSV-1 oncolytic virus targeting the degradation of GBP protein and its preparation and application, belonging to the field of gene engineering therapy. The present invention provides a method for improving the replication ability of oncolytic virus, which is achieved by targeting and inhibiting the GBP protein family. The present invention provides an HSV-1 oncolytic virus with strong replication ability, which is a recombinant HSV-1 expressing the Salmonella enterica serovar Typhimurium IpaH9.8 gene, and is obtained by inserting the IpaH9.8 gene fragment at the original ICP34.5 gene locus of HSV-1Δ34.5 / Δ47 (HSV-1 knocked out of the ICP34.5 and ICP47 genes). The oncolytic virus of the present invention can be used to prepare tumor therapeutic drugs, which can promote lymphocyte infiltration and killing activity, inhibit tumor growth, and has better therapeutic effects.

The present invention relates to immunogenic compositions comprising antigens from Salmonella enterica serovar Typhimurium (S. Typhimurium), Salmonella enterica serovar Enteritidis (S. Enteritidis), and Salmonella enterica serovar Typhi (S. Typhi). The present invention further relates to methods and uses of compositions comprising GMMA for boosting an immune response to an S. Typhi antigen, vaccines comprising the immunogenic compositions and methods and uses of the immunogenic compositions.

The invention relates to a beta-hairpin defense peptide as well as a derivative and application thereof, the beta-hairpin defense peptide is Jz-Def, and the amino acid sequence of the beta-hairpin defense peptide is as shown in SEQ ID No.1, specifically as follows: YCRRVCGRKRCFTYCRGK. The derivative of the beta-hairpin defensive peptide is defensive peptide Jz-M3, and the amino acid sequence of the defensive peptide Jz-M3 is as shown in SEQ ID No.2, specifically as follows: RWCRRFCRNGRCVRYCWR. The invention further discloses application of the beta-hairpin defense peptide and / or the derivative of the beta-hairpin defense peptide in preparation of bacteriostatic and bactericidal drugs or products. The beta-hairpin defensive peptide Jz-Def and the derivative defensive peptide Jz-M3 of the beta-hairpin defensive peptide Jz-Def and the derivative defensive peptide Jz-M3 of the beta-hairpin defensive peptide Jz-Def can kill clinical drug-resistant bacteria gram-positive pathogenic bacteria, gram-negative pathogenic bacteria and fungi at The bacillus amyloliquefaciens has strong antibacterial and bactericidal properties on providencia chinensis and other pathogenic bacteria in aquaculture and enterococcus faecalis, salmonella enterica and other putrefying bacteria in food, has very strong broad-spectrum antibacterial properties, has very strong high-temperature stability in antibacterial and bactericidal properties, has low hemolytic activity, and can be used for preparing the bacillus amyloliquefaciens. The composition has no obvious inhibiting and killing effect on phytobacterium and saccharomyces cerevisiae, and is high in safety.

The invention is concerning a medical preparation comprising bicyclic heptapeptides for use in vertebrates or humans suffering from an infection caused by Gram-negative bacteria like Pseudomonas aeruginosa, Klebsiella pneumoniae, Acineto-bacter baumannii, Neisseria gonorrhoeae, Chlamydia trachomatis, Shigella sonnei, Salmonella enterica Typhimurium LT2, Enterobacter spp., Bifidobacterium longum, Bacteroides fragilis, Lactobacillus reuteri, Enterococcus spp., Yersinia pestis and numerous other Gram-negative bacteria.

An immunogenic vaccine composition comprising a bivalent polysaccharide-protein conjugate vaccine comprising a Salmonella enterica serovar typhi saccharide-carrier protein and a Salmonella enterica se

The invention discloses a salmonella enterica ultra-rapid detection kit based on a whole-process nucleic acid detection chip and a detection method thereof. The kit comprises a rapid hot start DNA polymerase, a PCR reaction mixed solution, a positive reference substance and a negative reference substance, the kit also comprises the following primers which take the salmonella enterica V3-V4 region as a target sequence and are designed by a PCR fluorescent probe method based on a whole-process nucleic acid detection chip technical platform: an upstream primer F, a downstream primer R and a detection probe P, and the nucleotide sequences of the three primers are shown as SEQ ID NO. 1-3. According to the detection kit disclosed by the invention, a V3-V4 target sequence can be rapidly detected on the micro-fluidic chip within more than ten minutes, so that the accuracy and the specificity of detecting the salmonella enterica gene are ensured; the invention provides the salmonella enterica gene detection kit which is more comprehensive in detection effect, high in specificity, good in sensitivity, low in omission ratio, convenient, simple and easy to operate.

A method for increasing Salmonella outer membrane vesicle production includes the following steps: constructing knockout... tolR Recombinant Salmonella strains containing genes from wild-type Salmonella enterica SDC-11 tolR Obtained after gene knockout; will knockout tolR Recombinant Salmonella strains containing the gene were cultured in LB medium supplemented with glycine at 30–39°C for 2–24 h, and then outer membrane vesicles were collected. This invention constructs a knockout... tolR Salmonella strain sdc-11Δ tolR The OMV production was increased by 1.6 times. Based on this, the culture conditions of the strain were optimized by adding glycine to the culture medium, which further increased the OMV production by 8.8 times, confirming that culturing at 37℃ for 18 h was the optimal production condition. Compared with the wild-type strain sdc-11, the shape and particle size of the OMVs secreted by the recombinant strain under the above conditions were not significantly different.