The invention discloses a quadruple
fluorescence PCR method for detecting four types of
pathogenic bacteria. The four types of
pathogenic bacteria are
staphylococcus aureus,
salmonella enterica,
listeria monocytogenes and
vibrio parahemolyticus. Four elements, namely sample
DNA, a pair of universal primers and four probes,
fluorescence PCR premix liquid and
positive control are adopted in the method. The method comprises the following steps: designing the primers and the probes in accordance with 16S rRNA genes of the four types of
bacteria, implementing amplification on a target sequence and exciting corresponding
fluorescence signals; and preparing a kit from the to-be-detected sample
DNA in accordance with a
reagent formula and amplification conditions of the fluorescence PCR amplification; and specifically, the method comprises the following steps: establishing a quadruple fluorescence PCR primer
system for detecting the four types of
bacteria; establishing the quadruple fluorescence PCR kit for detecting the four types of
bacteria; and determining the quadruple fluorescence PCR identification method for detecting the four types of bacteria. The method provided by the invention has the advantages of being low in standard error, short in detection time, and being capable of simultaneously detecting a plurality of target genes and reducing
reagent cost; and the method is applicable to detection of
pathogenic bacteria in food and
cosmetics.