Culture medium for screening bacteria with siderophilic capacity and phosphate-solubilizing capacity simultaneously

A culture medium and capacity technology, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of low probability of success, long time required, low screening efficiency, etc., and achieve simple and operable preparation methods Strong performance, the effect of improving the efficiency of separation and screening

Inactive Publication Date: 2015-02-18
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, since the number of siderophilic bacteria or phosphate-solubilizing bacteria in the soil is not much, if the strains with both siderophilic and phosphate-solubilizing abilities are screened at one time, the probability of success will be lower, even if some strains have both siderophilic and phosphate-solubilizing abilities , its siderophilic or phosphate-dissolving ability is tested separately to verify whether the screened strains have other growth-promoting mechanisms, and it is not directly used as a screening condition, but two different growth-promoting mechanisms are used to detect or screen separately , it takes a long time, the steps are complicated, and the screening efficiency is low

Method used

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  • Culture medium for screening bacteria with siderophilic capacity and phosphate-solubilizing capacity simultaneously
  • Culture medium for screening bacteria with siderophilic capacity and phosphate-solubilizing capacity simultaneously
  • Culture medium for screening bacteria with siderophilic capacity and phosphate-solubilizing capacity simultaneously

Examples

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Effect test

Embodiment 1

[0033] A culture medium for screening bacteria with siderophilic and phosphate-dissolving ability, the formula for preparing 100mL of this medium contains the following components:

[0034] CAS blue detection solution 5mL, 1mmol / L CaCl 2 Solution 0.2mL, 1mmol / L MgSO 4 ·7H 2 O solution 0.2mL, acid hydrolyzed casein solution 6mL with a mass concentration of 10%, Ca 3 (PO 4 ) 2 0.5g, agar powder 2g.

[0035] The preparation method of the medium is as follows: first prepare 1mmol / L CaCl 2 solution, 1mmol / L MgSO 4 ·7H 2 O solution, acid hydrolyzed casein solution with a mass concentration of 10% (121°C, sterilized separately for 15 minutes); then take 0.2mL of CaCl 2 solution, 0.2 mL of MgSO 4 ·7H 2 O solution, 6 mL of 10% acid hydrolyzed casein solution, to which Ca 3 (PO 4 ) 2 0.5g, use biological buffer 1,4-piperazine diethanesulfonic acid [piperazine-N,N-bis(2-ethanesulfonic acid), Pipes], adjust pH 6.8~7.0. Dilute to 100mL with deionized water. Add 2g of agar po...

Embodiment 2

[0038] A culture medium for screening bacteria with siderophilic and phosphate-dissolving ability, the formula for preparing 100mL of this medium contains the following components:

[0039] CAS blue detection solution 3mL, 1mmol / L CaCl 2 Solution 0.3mL, 1mmol / L MgSO 4 ·7H 2 O solution 0.3mL, mass concentration 10% acid hydrolyzed casein solution 8mL, Ca 3 (PO 4 ) 2 0.3g, agar powder 3g.

[0040] The preparation method of the culture medium is the same as that in Example 1, and will not be repeated here.

Embodiment 3

[0042] A culture medium for screening bacteria with siderophilic and phosphate-dissolving ability, the formula for preparing 100mL of this medium contains the following components:

[0043] CAS blue detection solution 8mL, 1mmol / L CaCl 2 Solution 0.1mL, 1mmol / L MgSO 4 ·7H 2 O solution 0.1mL, mass concentration 10% acid hydrolyzed casein solution 4mL, Ca 3 (PO 4 ) 2 0.8g, agar powder 1g.

[0044] The preparation method of the culture medium is the same as that in Example 1, and will not be repeated here.

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Abstract

The invention discloses a culture medium for screening bacteria with siderophilic capacity and phosphate-solubilizing capacity simultaneously. The formula for preparing 100 mL of the culture medium contains the following components: 3-8 mL of CAS (Chrome Azurol S) blue detection liquid, 0.1-0.3 mL of 1 mmol / L CaCl2 solution, 0.1-0.3 mL of 1 mmol / L MgSO4.7H2O solution, 4-8 mL of acid hydrolyzed casein solution with the mass concentration of 8-12%, 0.3-0.8 g of Ca3(PO4)2 and 1-3 g of agar powder. The invention further discloses a preparation method of the culture medium. Through the adoption of the culture medium disclosed by the invention, bacterial isolates with siderophilic capacity and phosphate-solubilizing capacity simultaneously can be quickly obtained, so that the screening or detection time is shortened, and the screening efficiency is improved.

Description

technical field [0001] The invention relates to a culture medium for screening bacteria with siderophilic and phosphate-dissolving abilities. Background technique [0002] Phosphorus-solubilizing bacteria increase the absorbable phosphorus for crops by dissolving insoluble or insoluble phosphorus in the soil, improve the utilization efficiency of phosphorus in the soil, and promote plant growth. They are an important type of plant growth-promoting bacteria (Plant Growth-Promoting Bacteria, referred to as PGPB). [0003] By secreting siderophiles, siderophilic bacteria compete with pathogenic microorganisms for limited iron nutrition, inhibit the growth and reproduction of pathogenic microorganisms, and play a role in biological control. At the same time, they use iron chelated by siderophiles to improve plant iron nutrition and prevent plant deficiency. Iron chlorosis, promotes plant growth. [0004] In the prior art, siderophilic bacteria or phosphate-solubilizing bacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 余贤美艾呈祥王海荣安淼付丽刘嘉芬
Owner SHANDONG INST OF POMOLOGY
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