Method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu

Abstract

The invention discloses a method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu. According to the method, an improved QuEChERS method is adopted for extraction, further purification is not needed, high performance liquid chromatography is utilized for achieving separation of the fungal toxins, combined quadrupole-electrostatic field orbitrap mass spectrometry (Q-Orbitrab) is achieved, efficient parent ion selectivity and high-resolution accurate molecular weight (The mass number can be accurate to five digits after the decimal point.) are combined, accurate qualitativeness and quantification of the fungal toxins in baijiu and the original auxiliary materials can be achieved, and quantification is conducted through a matrix external standard method. The fungal toxins can be completely and effectively separated within 9 min, the linear relation of the fungal toxins within separate linear response ranges is good, the correlation coefficient R<2> is larger than 0.999, the lowest limit of quantification is lower than 1.0 microgram/L, the lowest detection limit is lower than 0.1 microgram/L, the recovery rate ranges from 70% to 120%, and the relative standard deviation (RSD) ranges from 0.5% to 4%. Compared with the national standard method, the method has the advantages of being rapid, simple, convenient, economical, efficient, safe and the like and can be suitable for separation and quantitative determination of various fungal toxins in baijiu and original auxiliary material samples.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for simultaneously detecting seven kinds of mycotoxins in liquor raw materials and auxiliary materials. Background technique [0002] Mycotoxins are a series of secondary toxic metabolites produced by various fungi under suitable environmental temperature and humidity conditions. More than 400 kinds of mycotoxins have been reported so far, most of which have high biological toxicity, can suppress the immune system, and have adverse health effects such as carcinogenicity, teratogenicity, and impact on growth and development. Mycotoxins are widely found in grains, while traditional Chinese liquor is made of grains such as sorghum, wheat, barley, corn, and rice as main raw materials, bran and rice husks as auxiliary materials, and distiller's yeast as the main source of microorganisms and enzyme preparations. Made from grains such as barley, wheat, and peas. ...

AI Technical Summary

Problems solved by technology

The biological identification method has lower requirements on sample purity, mainly qualitative analysis, which has low specificity, low sensitivity, high cost, and long experimental period, and is generally only used as evidence for chemical analysis methods

Immunoassay has the characteristics of high specificity, high sensitivity, stability, speed, and convenience, and can be applied at different levels and levels of tissues, cells, and molecules. However, some mycotoxins are difficult to couple with carrier proteins, and the structure There are many similarities and cross-reactions are common. This method has certain false positives and cannot be used as a final confirmation method.

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