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Acinetobacter strain and application thereof to degradation of zearalenone

A technology of zearalenone and bacillus, which is applied in the fields of application, bacteria, animal feed, etc., can solve the problems of detoxification, etc., and achieve the effect of strong degradation ability, low cost and high efficiency of secondary pollution

Inactive Publication Date: 2012-07-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, after analyzing the found microbial metabolites, it is found that most of the bacteria convert ZEN into α or β type ZEN or zearalenol, and the estrogenic toxicity of these products is similar to or worse than that of ZEN. Degradation cannot be called true detoxification

Method used

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  • Acinetobacter strain and application thereof to degradation of zearalenone
  • Acinetobacter strain and application thereof to degradation of zearalenone
  • Acinetobacter strain and application thereof to degradation of zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation and identification of strain Acinetobacter sp.SM04

[0026] 1. Isolation of strain Acinetobacter sp.SM04:

[0027] Take 2.0g of soil (plow layer soil in the corn base of Dengta Basin, Heyuan City, Guangdong Province) soaked with 10mL sterile 0.15mol / L sodium chloride aqueous solution, and take 0.15mL supernatant to inoculate into 25μg / mL ZEN and 15μg / mL ZEN mL nystatin was enriched in 3mL M1 medium, and the remaining ZEN in the culture was ultrasonically extracted with an equal volume of methanol, and the residual amount of ZEN was quantitatively analyzed by HPLC-PAD.

[0028]Inoculate 0.5 mL of the culture with no residual ZEN detected into 3 mL of M2 medium (ZEN as the sole carbon source) and incubate for 48 hours, then take 100 μL of the culture and serially dilute it with sterile 0.15 mol / L sodium chloride aqueous solution, and take 100 μL The diluted solution was coated on a nutrient broth plate, placed in a constant temperature incubator at 30°C for 24 ...

Embodiment 2

[0046] Degradation test of Acinetobacter sp.SM04 on ZEN:

[0047] (1) Prepare the degradation test medium containing ZEN 25mg / L: add quantitative ZEN methanol stock solution in a sterilized plastic centrifuge tube, in N 2 After methanol was removed under the evaporator, sterilized M1 medium was added to make the final concentration of ZEN 25mg / L.

[0048] (2) Culture the Acinetobacter sp.SM04 pure bacteria of Example 1 on the M1 plate, then pick a single colony from the solid M1 plate and inoculate it into 30mL of the degradation experiment medium, and cultivate it in a water bath shaker at 30°C (200r / min). After culturing for 12 hours, use an equal volume of methanol to ultrasonically extract the residual ZEN in the culture, and use HPLC-PAD to quantitatively analyze the residual ZEN in the centrifuged supernatant. In the experiment, three parallel samples were made, and the uninoculated degradation test medium was used as control sample.

[0049] (3) Determination of the...

Embodiment 3

[0052] Degradation test of Acinetobacter sp.SM04 on ZEN:

[0053] The steps, methods and materials of this example are the same as in Example 2, except that the incubation time in step (2) is 24 hours.

[0054] Test results: After 24 hours, the concentrations of ZEN in Acinetobacter sp.SM04 culture solution were 4.82mg / L, 4.97mg / L, 5.26mg / L, and the average value was 5.02mg / L. After culturing for 24 hours, the color of the culture solution further showed a more obvious difference, from light yellow to dark yellow, while the control sample did not change significantly.

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Abstract

The invention discloses an acinetobacter strain and application thereof to degradation of zearalenone. The acinetobacter strain disclosed by the invention is Acinetobacter sp. SM04, and is preserved in the China General Microbiological Culture Collection Center on December 5th, 2011, and the preservation number is CGMCC No. 5524. The Acinetobacter sp. SM04 disclosed by the invention has stronger degradation capability towards mycotoxins ZEN, can degrade more than 98% of zearalenone into low-estrogenic-activity product within 36 hours, cannot produce high-estrogenic-activity analogues such as zearalenone and zeranol, and has real detoxification capability. The acinetobacter strain can be applied to the treatment of corn grains, corn alcohol residues or other mycotoxin contaminated feed, so that safe food and animal feed are obtained, and further, a zearalenone degradation strain which has low cost and high efficiency and avoids secondary pollution is provided for the environment-friendly processing and treatment of the grains and feed.

Description

technical field [0001] The invention belongs to the field of microorganism application, and in particular relates to an Acinetobacter sp.SM04 strain and its application in degrading zearalenone. Background technique [0002] Zearalenone (ZEA, ZEN) is an estrogenic mycotoxin with a dihydroxybenzoic acid lactone structure, which is produced by Gibberella zeara, Fusarium graminearum, and Fusarium tritina, and mainly pollutes wheat , barley, oats, corn and other crops. About 25% of crops and grains in the world are polluted by mycotoxins every year, and my country's food and feed industry loses more than 10% of its total output due to mildew every year. Sampling and testing from national warehouses and feed factories found that the detection rate of ZEN in corn and other crops and feed was as high as 100%, and the detection concentration exceeded the standard seriously. ZEN can cause premature maturation in breeding pigs or poultry, disordered reproductive cycle, and bring hug...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015A23K1/00C12R1/01A23L5/20
Inventor 唐语谦吴晖余元善肖俊梅肖性龙陈艺李晓凤
Owner SOUTH CHINA UNIV OF TECH
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