75 results about "Reproductive cycle" patented technology

A method for tissue bioassay comprising the steps of placing in the vagina of a female mammal an elongated probe having at least two electrodes positioned close to an insertion end of the probe, none of the electrodes extending around the entire periphery of said probe and at least one of then being metallic, applying across the electrodes an alternating voltage of relatively low peak-to-peak amplitude, measuring across the electrodes a value representative of the phase of the reproductive cycle of the female or the hormonal status of the fornix epitheliurn, and comparing that value with at least one reference value. A probe for practicing this method also is disclosed.

Methods and apparatus for interpreting vaginal measurement data by electronic means without the need for the user to take part in the data interpretation process. More specifically, an intelligent probe monitors folliculogenesis and correlates the ovarian function data to probe profile characteristics of the female reproductive cycle. A fertile window is defined with reference to the correlation between folliculogenesis and probe cyclic profiles. Data stored within the apparatus can be downloaded into an external display appliance or data analysis unit. Another aspect of the invention allows for continuous layout of the memory of preceding cycles in the inventory of the apparatus, retaining the most recent while erasing the most distant stored data. These methods assure a reliable fit for the diagnosis of fertility, treatment of infertility as far as timing of treatment procedures, management of subfertility and other gynecological disorders such as luteal phase deficiency or short luteal phase, and the management of premenstrual mood disorders, and of premenopausal and menopausal patients.

The invention discloses a factory production method of a dendrobium officinale seedling, which comprises the steps of explant selection, pretreatment, sterilization, lateral bud germination, protocorm inducement, subculture, protocorm differentiation culture, strong seedling rooting culture, and bottle seedling transplant, and a specially-prepared culture medium is used in each step for culturing. The method has the benefits that an explant is easy to take; the sterilization efficiency is high; the growth coefficient is high and stable; components of the culture media are unique, concise and practical; the reproductive cycle is short; the production cost is low; and the produced seedling is stable in hereditary character, strong and tidy, and strong in growth vigor.

A system and method of release and dispersion of genetically altered flies or other insects to control insect population. Disclosed is an apparatus designed to be carried by an aircraft which implements a method of insect population control. The apparatus features one or more removable fly chambers that contain fly containment cylinders. The fly chambers are seated upon one or more nesting chutes. A refrigeration system operates to physically retard the fruit flies. Once the flies have been successfully immobilized by the refrigerated air, they are released from the fly containment cylinders and fall to the bottom of the fly chamber. Gates on the bottom of the fly chamber are opened manually or remotely to allow the refrigerated flies to fall through the nesting chute into the spiral release controllers. The spiral release controllers dispense the genetically altered flies into the exit chute. The genetically altered flies travel through the exit chute, fall to the earth below and mate with the population thus serving to decrease population by capitalizing on a weakness in the reproductive cycle of the fruit fly or other insect.

The invention discloses a bletilla striata cultivation method which is composed of forest land selection, land preparation, transplantation and cultivation management and the like. Compared with the prior art, according to the bletilla striata cultivation method, the under-forest space can be utilized effectively, the planting cost is reduced, the forest land unit area earning is improved, the planting cycle can be shortened, and bletilla striata quality and yield are improved. By utilizing cultivated seedlings of bletilla striata, the shortcomings that the tuber reproductive cycle is long, the reproductive efficiency is low, the seed consumption amount is large, the planting cost is high and plenty of artificial cultivation cannot be met easily can be overcome effectively. In addition, according to the method, agricultural straw and rice husk return to fields, not only can the environmental pollution caused by straw incineration be avoided, but also the soil structure can be changed, the soil fertility is improved, and the damage to the ecological environment caused by the use of chemical fertilizer is avoided.

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

A device for interrupting the gestating cycle of a mosquito includes a container having a bottom wall and side walls. A generally porous sheet of material is placed over the upper opening thus formed in the container by securing it to the periphery of the container. Double sided tape, mechanical clips, etc. may be used to attach the porous material to the periphery of the container to cover the open area. The porous sheet of material is farametis and contains apertures that are small enough to prevent an adult mosquito from passing through it but yet allow evaporation of water placed into the container and water molecules through the apertures. A sticky substance is placed on the outer surface of the porous sheet. Water is evaporated from the container and water molecules are released through the apertures into the atmosphere above the porous sheet. Gestating mosquito's are attracted to the device and attempt to enter the container to lay there eggs in the water within the container but can not. Instead, the mosquitoes are immobilized on the surface of the porous material when retained by contact with a sticky substance and prevent it from laying eggs in the container or any other body of water. This breaks the reproductive cycle of the mosquito.

The invention provides a duck egg yolk anti-freezing agent for cryopreservation of donkey semen and a preparation method thereof. Each 100 milliliters of anti-freezing agent contains the following components: 3 to 4 grams of Tris, 1 to 2.5 grams of glucose, 1.5 to 3.0 grams of citric acid, 5 to 10 milliliters of glycerol, 10 to 30 milliliters of yolk, 450 to 550 thousand international units (IU) of penicillin, 45 to 55 thousand IU of streptomycin, and the balance of distilled water. The duck egg yolk anti-freezing agent solves the problems of long reproductive cycle of female animals, low artificial insemination rate, complex production operation of frozen donkey semen and high difficulty in the prior art.

The invention discloses a tissue culture method of America Lagerstroemia indica Red Rocket. The method includes disinfection, sterilization, initial culture, subculture, rooting culture and hardening seedling and transplanting. America Lagerstroemia indica Red Rocket annual shoot as a explants is subjected to aseptic inoculation for inoculation to bud inducing culture medium, so as to form cluster buds; cluster buds are subjected to subculture, and then transferred to a rooting culture medium for rooting induction, in order to form rooting seedlings; the rooting seedlings are subjected to hardening seedling domestication to obtain regenerated plants. The method of the invention realizes easy breeding of the America Lagerstroemia indica Red Rocket, breaks the seasonal and climate limit, and has shortened reproductive cycle and improved survival rate of regenerated plants.

The invention discloses a white cherry blossom tissue culture and rapid propagation method to meet the market needs by carrying out industrialized production. The white cherry blossom tissue culture and rapid propagation method comprises the following steps of cherry blossom aseptic seedling acquisition, aseptic seedling subculture, rooting culture and hardening-seedling and transplanting of tissue culture seedlings. According to the white cherry blossom tissue culture method, aseptic explant acquisition and aseptic seedling induction culture use a same medium, so that the operation flow is simplified, and the production cost is lowered. With adoption of the method, a set of complete white cherry blossom tissue culture production flow can be established within 4-5 months, and thus industrial production is carried out, the reproductive cycle of tissue culture is greatly shortened, the rooting rate is high and can reach 98 percent or more, and the possibility of rapidly supplying a great amount of cherry blossom seedlings in the market is provided.

The invention relates to the technical field of plant cultivation, in particular to the technical field of plumbago auriculata cuttage. The technical scheme aims at providing a cuttage propagation method for a plumbago auriculata. The cuttage method of the plumbago auriculata comprises the steps of shearing middle-upper hard stems of the plumbago auriculata as cutting slips with the length of 10-12cm; preparing a transplanting bed matrix by roseite and river sand with the preparation proportion of 1:1; after soaking the hard stems of the plumbago auriculata into a solution containing 500mg/L of IAA (Indoleacetic Acid), 500mg/L of IBA (Indole Butyric Acid), and 1000mg/L of NAA (Naphthalene Acetic Acid) for 30 seconds, planting the hard stems of the plumbago auriculata into the transplanting bed, and pouring water over the hard stems. The cost of the plumbago auriculata cut by the method can be greatly reduced, the rate of survival is high and can reach up to 92.22%, the rooting time is earlier, the growth and development is fast, and the reproductive cycle of the plumbago auriculata can be reduced, so that a new high-efficient propagation method can be provided for the production of the plumbago auriculata; the cuttage propagation method is simple and feasible, and is easy to popularize.

The present invention provides a sow miscarriage prevention feed, which is characterized by comprising the following components in part by weight: 0.5 to 1.5 parts of baical skullcap root, 0.5 to 1.5 parts of Chinese taxillus twig, 0.5 to 1.5 parts of membranous milkvetch root, 0.5 to 1.5 parts of flowery knotweed stem, 0.5 to 1.5 parts of indigowoad root, 0.5 to 1.5 parts of tangerine peel, 0.5 to 1.5 parts of cyrtomium rhizome, 0.5 to 1.5 parts of caulis perllae, and 0.5 to 1.0 part of adhesive. The sow miscarriage prevention feed has the advantages of ensuring replacement sows and multiparous sows to smoothly enter the next reproductive cycle while meeting normal physiological needs, and improving the production performance of the sows. Meanwhile, the sow miscarriage prevention feed meets the demands of large-scale pig raising, carries on the concept that prevention is more important than treatment, reduces medication cost and improves overall pig raising benefit.

The invention provides a casein anti-freezing agent for cryopreservation of donkey semen and a preparation method thereof. Each 100 milliliters of anti-freezing agent contains the following components: 1.0 to 3.0 grams of casein, 2.0 to 3.0 grams of glucose, 1.0 to 2.0 grams of lactose, 3.0 to 6.0 grams of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) BUFFER, 2.0 to 3.0 milliliters of glycerol, 2 to 4 milliliters of yolk, 450 to 550 thousand international units (IU) of penicillin, 4.5 to 5.5 grams of gentamicin, 20 to 30 milligrams of amphotericin B, and the balance of HANK solution. The casein anti-freezing agent solves the problems of long reproductive cycle of female animals, low artificial insemination rate, complex production operation of frozen donkey semen and high difficulty in the prior art.

The present invention relates to methods, compositions and kits for controlling, managing or manipulating the non-human mammalian female reproductive cycle. The methods include oestrus induction and/or synchronisation, induction of ovulation and/or superovulation and optionally further includes a method of providing an immunopermissive uterine environment prior to insemination or implantation of embryos.

The invention relates to a cultivating method for improving multiplication coefficient of a shoot, particularly to a cultivating method for improving multiplication coefficient of an acer mono shoot, which comprises the steps follows: 1), washing a resting shoot from an acer mono adult seed tree; 2), sterilizing a stem; 3), cultivating the sterilized stem to obtain the steam with a shoot; 4), cultivating the stem with the shoot to obtain the extended immature stem; and 5), cultivating the extended immature stem to obtain a plantlet. By adopting the resting sprouting shoot of the acer mono wild tree as the material, the research on the shoot multiplication and cultivation conditions are performed, and the cluster shoot induced cultivation system for directly multiplying the acer mono shoot is established, so as to lay a foundation of acer mono in vitro rapid propagation system. The reproductive cycle is short, the efficiency is high, the auxiliary shoot germination rate of the extended immature stem can reach 63.3%, the average height can reach 15.9 mm; and the shoot multiplication rate of the plantlet can reach 90%, the multiplication times can reach 3.19, and the shoot growth is normal.

The invention relates to a treatment method of chili pepper seeds with high germination rate. The treatment method specifically comprises the following steps: (1) soaking chili pepper seeds into a fulvic acid potassium water solution in a temperature holding manner, fishing out a filtrate, and performing magnetization treatment for the first time; (2) freeze-drying until the moisture content is 18%-22%, soaking in a seed soaking chemical, fishing a filter reagent out, and performing magnetization treatment for the second time, wherein the seed soaking chemical is prepared from the following raw materials: zinc sulfate, sophocarpidine, chromium trioxide, puerarin, L-alanine and water; and (3) freeze-drying until the moisture content is 12%-16%. Not only can the enzymatic activity of chili pepper seeds be effectively increased to provide energy for germination of the seeds, so that the seeds can germinate more rapidly with high survival rate, enhance the germination potential of the chili pepper seeds so as to make the chili pepper seeds germinate easily with high germination rate, but also the cold-resistant and disease and pest-resistant functions of the chili pepper can be enhanced, the rooting and seedling and strong seedling culturing are facilitated, the reproductive cycle is shortened, thus promoting the high yield of chili peppers.

The invention discloses a cyclopeptide compound and a preparation method and application thereof. The cyclopeptide compound is characterized in that the structural formula is as shown in I. The preparation method comprises the following steps: fermental cultivation is performed on Cladosporium sphaerospermum, then the obtained fermentation product is extracted by use of ethyl acetate to obtain a crude extract, and the crude extract is subjected to reduced-pressure silica gel column chromatography, Sephadex LH-20 gel column chromatography and reversed-phase preparative high performance liquid chromatography separation and purification to obtain the cyclopeptide compound. The cyclopeptide compound has the alpha-glucosidase inhibition function, and also has the advantage that the cyclopeptide compound can serve as a drug lead compound for treating diabetes and obesity when combined with various pharmaceutically acceptable carriers, excipients or auxiliary materials. The preparation method is low in cost, short in reproductive cycle and simple in purification process.