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Tissue culture method of America Lagerstroemia indica Red Rocket

A red rocket crape myrtle and tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, easy to be restricted by seasons, etc., achieve easy reproduction, shorten the reproductive cycle, and increase the survival rate Effect

Active Publication Date: 2014-11-05
中林绿苑江苏生态环境有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The tissue culture method of American red rocket crape myrtle is a commonly used breeding method, but the existing tissue culture method has a low survival rate and is easily restricted by seasons

Method used

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Embodiment Construction

[0011] Below in conjunction with embodiment the present invention will be further described.

[0012] A method for tissue culture of American red rocket crape myrtle, comprising the following steps:

[0013] 1) Disinfection and sterilization: Select the tender branches of American red rocket crape myrtle in the same year, soak them in bleach for 10 minutes, rinse them in running water for 20 minutes, soak them in 75% alcohol for 30 seconds on the ultra-clean workbench, and then transfer them to 0.1% mercuric chloride Sterilize in the solution for 10 minutes, pour off the sterilizing solution, rinse 4 to 5 times with pre-prepared sterile water, drain the water, inoculate the sterilized twigs on the bud-inducing medium;

[0014] 2) Carry out inoculation culture: cut the selected explants into small pieces of 1-2 cm and inoculate them on the prepared MS medium, and cultivate them for about 25-30 days. The composition of the inoculation medium is: MS basic medium, plus 2 ,4—D 1.5...

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PUM

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Abstract

The invention discloses a tissue culture method of America Lagerstroemia indica Red Rocket. The method includes disinfection, sterilization, initial culture, subculture, rooting culture and hardening seedling and transplanting. America Lagerstroemia indica Red Rocket annual shoot as a explants is subjected to aseptic inoculation for inoculation to bud inducing culture medium, so as to form cluster buds; cluster buds are subjected to subculture, and then transferred to a rooting culture medium for rooting induction, in order to form rooting seedlings; the rooting seedlings are subjected to hardening seedling domestication to obtain regenerated plants. The method of the invention realizes easy breeding of the America Lagerstroemia indica Red Rocket, breaks the seasonal and climate limit, and has shortened reproductive cycle and improved survival rate of regenerated plants.

Description

technical field [0001] The invention relates to the field of plant asexual reproduction, in particular to a tissue culture method of Lagerstroemia crape myrtle. Background technique [0002] American red rocket crape myrtle is one of the most bright red varieties of crape myrtle. The flowers are as bright red as the national flag. The flower clusters can reach 24 inches. The flowers are scarlet on sunny and hot days, slightly lighter on cloudy and cool days, and may have white spots. The new leaves are slightly red, and the old leaves are green with a slight blush. The leaf buds are dark red before flowering, and turn cherry red when flowering. The initial flowering in June can continue to bloom until frost, and can be pruned during the flowering period to bloom multiple times. Wide adaptability and strong stress resistance. It is suitable for solitary planting and group planting, and can be cultivated as flower hedges, and can be used to make bonsai and pile landscape...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 蔡鸿宇史云光张荣良戴静查立胜
Owner 中林绿苑江苏生态环境有限公司
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