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White cherry blossom tissue culture and rapid propagation method

A tissue culture and cherry blossom technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long production cycle and high production cost of tissue culture seedlings, and achieve the advantages of promoting industrial production, increasing the number of inoculations, The effect of reducing the production cost per plant

Active Publication Date: 2017-03-29
上海菁艺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to provide a method for tissue culture of white cherry blossoms, which solves the problems of long production cycle and high production cost of tissue culture seedlings

Method used

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  • White cherry blossom tissue culture and rapid propagation method
  • White cherry blossom tissue culture and rapid propagation method
  • White cherry blossom tissue culture and rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] White cherry tissue culture and rapid propagation method, it carries out according to the following step order:

[0037] (1) Obtaining of aseptic seedlings of white cherry blossoms:

[0038] Cut off the annual healthy young branches of white cherry blossoms, cut off the petiole and leaves at a distance of 2-3mm from the base of the petiole, and trim the branches into 3-5cm long branches, soak in 2% washing powder for 3-5min, and rinse with running water for 30- 60min, then disinfect with 75% alcohol for 20-30s on the ultra-clean workbench, rinse with sterile water for 1-2 times, then disinfect with 0.05% mercury liter for 8-16min, rinse with sterile water for 6-8 times, and blot dry with filter paper After watering, cut the stem tip or stem section with one or two axillary buds and inoculate them into the culture medium, cultivate them in the dark for 5-7 days, and then transfer them to normal light for cultivation. After 10-12 days, the axillary buds begin to germinate...

Embodiment 2

[0046] Optimization of basic medium for induction and subculture. In order to improve the induction rate and proliferation coefficient of white cherry blossoms, the basic medium for induction culture and subculture was improved and optimized. The specific components of the improved MS mother solution were as follows:

[0047] CaCl 2 2H 2 O 441mg / L; MgSO 4 ·7H 2 O 370mg / L; KNO 3 2022mg / L; NH 4 NO 3 1600mg / L; NaH 2 PO 4 312 mg / L; Na 2 EDTA 37.25mg / L; FeSO 4 ·7H 2 O 27.8mg / L; KI 0.83mg / L; H 3 BO 3 6.2mg / L; MnSO 4 4H 2 O 16.9mg / L; ZnSO 4 ·7H 2 O 11.5mg / L; Na 2 MoO 4 2H 2 O 0.25mg / L; CuSO 4 ·5H 2 O0.03mg / L; CoCl 2 ·6H 2 O 0.03mg / L; inositol 100mg / L; niacin 0.5mg / L; pyridoxic acid hydrochloride 0.5mg / L; thiamine hydrochloride 0.1mg / L; glycine 2mg / L.

[0048] Except that the basic medium for induction and subculture was replaced by the improved MS mother solution, other steps and methods were the same as in Example 1. Using the improved MS as the basic medi...

Embodiment 3

[0050] The screening of the starting medium was based on the improved MS as the basic medium, and L8 (2 7 ) orthogonal design (as shown in Table 1), to screen the effects of different types and concentrations of hormones on the induction rate of white cherry blossoms. Each treatment was inoculated with 20 bottles, and each bottle was inoculated with 3 plants, repeated 3 times. All the other method steps are the same as in Example 1.

[0051] Table 1 Effects of different types and concentrations of hormones on the induction rate of white cherry blossoms

[0052]

[0053] The test results are shown in Table 2:

[0054] Table 2 Effects of different types and concentrations of hormones on the induction rate of white cherry blossoms

[0055]

[0056] It can be seen from the above table that different types and concentrations of hormones are used to induce culture of white cherry blossoms, and the induction rate is different. Among them, GA3 had the most significant inducti...

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Abstract

The invention discloses a white cherry blossom tissue culture and rapid propagation method to meet the market needs by carrying out industrialized production. The white cherry blossom tissue culture and rapid propagation method comprises the following steps of cherry blossom aseptic seedling acquisition, aseptic seedling subculture, rooting culture and hardening-seedling and transplanting of tissue culture seedlings. According to the white cherry blossom tissue culture method, aseptic explant acquisition and aseptic seedling induction culture use a same medium, so that the operation flow is simplified, and the production cost is lowered. With adoption of the method, a set of complete white cherry blossom tissue culture production flow can be established within 4-5 months, and thus industrial production is carried out, the reproductive cycle of tissue culture is greatly shortened, the rooting rate is high and can reach 98 percent or more, and the possibility of rapidly supplying a great amount of cherry blossom seedlings in the market is provided.

Description

technical field [0001] The invention relates to a method for tissue culture, in particular to a method for tissue culture and rapid propagation of white cherry blossoms, and belongs to the technical field of tissue culture and seedling cultivation. Background technique [0002] Cherry blossoms belong to the genus Prunus in the Rosaceae Li subfamily, and are famous woody ornamental flowers. Originated in China, it is an excellent tree species rich in resource species and widely distributed in our country. About 45 species are planted in all places except the northeast and northwest regions, and also grow all over the world. It is the national flower of Japan. Most of the cherry blossoms are deciduous trees, 5-25 meters high, with a wide crown, gray-brown bark, solitary, scattered or few flowers (usually less than 8) forming a corymb raceme. Cherry blossoms are not only suitable for garden landscaping, but also can be used as street trees. It is better to plant them in patche...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张玉杰景龄冒文娟李文剑林大为张承妹苏涌
Owner 上海菁艺生物科技有限公司
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