Method for evaluating toxicity of organic pollutants of reclaimed water based on caenorhabditis elegans

Abstract

The invention discloses a method for evaluating toxicity of organic pollutants of reclaimed water based on caenorhabditis elegans. The method comprises the following steps: after enriching the organicpollutants in the reclaimed water and filtering bacteria, dissolving the reclaimed water in a salt solution; respectively exposing L4-stage caenorhabditis elegans in a blank salt solution and the salt solution containing the enriched organic pollutants of the reclaimed water; respectively measuring the number of spawns; performing significant difference analysis on the number of spawns; combiningwith enrichment times; and utilizing a toxicity evaluation rule of the organic pollutants of the reclaimed water to obtain a toxicity level. The method provided by the invention utilizes characteristics of simple structure, easy culture, short reproductive cycle and small individual difference of the caenorhabditis elegans, combines enrichment of the organic pollutants with bacterial filtering treatment to evaluate the toxicity of the reclaimed water, has advantages of being high in efficiency, simple in operation, low in cost and easy to promote, maintains balance of osmotic pressure of a cell system of the caenorhabditis elegans, inhibits incubation of progeny spawns by dropwise adding 5-fluorouracil, and has improved accuracy and simplicity, thereby being wide in application prospect.

Description

Technical field [0001] The invention relates to the technical field of environmental toxicology detection, in particular to a method for evaluating the toxicity of organic pollutants in regenerated water based on Caenorhabditis elegans. Background technique [0002] With the increasing development of industry and the improvement of the level of urbanization, the discharge of sewage is increasing. As a renewable resource, it can be turned into a beneficial water resource if it is properly treated to meet the water quality standards required by the target use. Reclaimed water can be reused in fields such as farmland irrigation, industrial cooling, river landscape, landscaping, and municipal miscellaneous use. However, because the reclaimed water comes from domestic sewage and industrial wastewater, although the pollutant concentration is significantly reduced after the secondary treatment, trace chemical pollutants still exist. In addition, because the chemical pollutants in the re...

AI Technical Summary

Problems solved by technology

In the biotoxicity method, model organisms such as algae, daphnia, zebrafish, and luminescent bacteria are widely used in the detection of water quality toxicity in wastewater, sewage, and surface water. However, the biological sensitivity of fish including zebrafish is low, and the incubation time It is long and suitable for the toxicity determination of a single chemical substance; algae also have the disadvantages of long cultivation time and complicated operation; although Daphnia magna is easy to cultivate and low in cost, its sensitivity is average; although luminescent bacteria have high sensitivity and short operation time, their Difficult to cultivate, often need to buy, high testing cost

[0004] As an internationally recognized multicellular model organism, Caenorhabditis elegans has the characteristics of simple structure, easy cultivation, short reproductive cycle and small individual differences, which is conducive to the standardization and normalization of toxicity evaluation. It is widely used, but there is no report on how to evaluate the toxicity of organic pollutants in reclaimed water based on Caenorhabditis elegans

Images