15 results about "Luminescent bacteria" patented technology

The invention discloses a device for gradient dilution of luminous bacteria concentration, which comprises a dilution container device 1 and a power stirring device 7, the dilution container device 1 comprises a circular bottom plate 4 and a plurality of cylindrical partition plates 2 with different radiuses, the circular bottom plate 4 is provided with a plurality of annular tracks 6, the partition plates 2 and the corresponding annular tracks 6 are mutually clamped, and the power stirring device 7 is connected with the power stirring device 7. A closed container is formed, a stirring rod 5 is further arranged on the circular bottom plate 4, and the power stirring device 7 provides power for the stirring rod 3. According to the device, personal errors can be reduced; rapid gradient dilution is realized, and the experiment efficiency is improved; the dilution ratio is accurately controlled, and the accuracy of experimental results is ensured; operation is simple and adaptability is high; the cost is reasonable.

The invention discloses a recombinant photobacterium for detecting genotoxic pollutants as well as a preparation method and a detection method of the recombinant photobacterium, and belongs to the technical field of analysis and detection. The invention provides a recombinant luminescent bacterium for detecting genetic toxic pollutants, a chromosome of the recombinant luminescent bacterium carries a luminescent gene, and the luminescent gene and a target gene on the chromosome have a common promoter. Luminescence enhancement of the recombinant luminescent bacterium is induced by the genotoxic substance, and target gene transcription of the recombinant luminescent bacterium is enhanced in the presence of the genotoxic substance, so that the luminescent gene is expressed, and whole-cell biosensing of genotoxicity detection is realized. The recombinant photobacterium obtained by the method has the advantages of stable strain structure, high sensitivity, simple sample treatment, fast detection, low detection cost, and detection limit of 0.5 [mu] M.

The present invention discloses an automatic device for monitoring and warning the influent water quality of a sewage treatment plant, and relates to the technical field of sewage treatment. The device is arranged at the upstream pipe network of the sewage treatment plant, and can automatically sample sewage from the pipe network at regular intervals, and monitor the water quality parameters of the influent pipe network. Through parameters such as the ammonia nitrogen removal rate before and after sludge nitrification aeration, the strength of biological toxic substances in the influent water quality is quantified, and the warning threshold is set. The warning data is transmitted in real time through the PLC control system and the wireless transmission system. After computer-side data diagnosis, the influent water quality of the sewage treatment plant can be monitored and warned throughout the day. This device can not only warn of high-concentration COD and ammonia nitrogen, but also warn of sewage with high biological toxicity. Compared with the sludge respiration rate method and the luminous bacteria method on the market, it has stronger applicability, intuitive effect, stable operation, quick and easy operation, and good repeatability and stability.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

This application provides a water quality biotoxicity detection chip, its preparation method, and a detection system. The water quality biotoxicity detection chip includes: a chip body, microchannels located inside the chip body, and multiple micropore structures. The micropore structures include: a first injection port for injecting a first liquid; a second injection port for injecting a second liquid; and a drain port for discharging liquid. The microchannels include: a mixer connected to the first injection port via a first injection pipe and connected to the second injection port via a second injection pipe; at least a partially transparent detection ring, which is connected to the reaction channel via the drain pipe and drain port, located between the mixer and the detection ring; and several reaction columns spaced apart along the extension direction of the reaction channel. Using a microfluidic detection chip for water quality biotoxicity detection based on luminescent bacteria can reduce the amount of bacterial strain used in each detection and shorten the reaction time during detection.

The invention relates to a water quality acute toxicity detection device, and belongs to the technical field of water quality toxicity detection devices.The water quality acute toxicity detection device comprises a device body, a detection tank is fixedly connected to the outer wall of the device body, a connecting cylinder is fixedly connected to the top of the detection tank, and a collecting tank is fixedly connected to the outer wall of the connecting cylinder through a connecting pipe; a luminous bacterium storage tank located on one side of the collecting tank is fixedly connected to the outer wall of the connecting cylinder through a connecting pipe, a connecting pipe is slidably connected to the outer wall of the collecting tank, and a sample injection head is fixedly connected to one end of the connecting pipe. According to the requirement of the detection device for water quality toxicity detection characteristics, through the arrangement of the self-adaptive dredging assembly, the situation that when water quality is sampled at a multi-channel sample inlet, the surface of a filter screen is blocked, and the water resource sample introduction rate is affected is reduced, and meanwhile under the impact of water pressure and connection of a reset spring, the water quality toxicity detection effect is improved. And the scraping plate synchronously vibrates, rotates and scrapes the surface of the filter screen, so that the continuity and dredging effect of the detection device on water resource sampling are improved.

The invention discloses a method for measuring the long-term toxicity of antibiotics by using luminous bacteria, which comprises the following steps: S1, culturing strains to obtain a freshly prepared luminous bacteria solution; step S2, pre-experiment: determining a luminescence curve of the luminescent bacteria by adopting the luminescent bacteria liquid freshly prepared in the step S1; s3, performing toxicity test, namely performing the toxicity test in a light-emitting upstream section of the light-emitting bacteria; the monitoring starting point is selected from the time when the luminous bacteria reach 10% of the luminous peak value, and the error is allowed to be 0.5 h before and after; a monitoring end point is selected to be delayed within 1h when the luminous bacteria reach a luminous peak value; and S4, measuring antibiotic toxicity by using the inhibition ratio of the antibiotic to luminescence. The contribution of the invention lies in providing a monitoring start point and a monitoring end point capable of ensuring the test accuracy, verification tests find that the luminescence inhibition factors of the luminescence downstream section of the luminescence bacteria are many, the test result is inaccurate, and the luminescence upstream section can fully characterize the toxicity of antibiotics.

The invention discloses application of luminous bacteria in preparation of index organisms for evaluating the effect of a disinfectant and a rapid evaluation method for the effect of the disinfectant.

This invention discloses a method for detecting chiral substances using a luminescent bacteria array sensor. The method involves mixing the luminescent bacteria array sensor with the sample to be tested, followed by absorption spectral scanning to obtain the spectral data of the test solution. Linear discriminant analysis (LDA) is then performed on the spectral data. By comparing the positions of unknown sample data points in the LDA spectrum with data points of known chiral types, the type, composition, and content of chiral substances in the test solution can be determined. The luminescent bacteria array sensor is composed of multiple luminescent bacteria. This invention also discloses its application in chiral substance detection. The method provided by this invention is used for chiral identification. Through LDA processing of matrix data, it correctly identifies chiral substances of different chiralities and concentrations, and the mechanism of this identification is preliminarily explored. This method is simple to operate, can be used for rapid chiral detection, and has certain practicality and application prospects.

To provide an ecotoxicity monitoring device using a luminescent bacterium and an ecotoxicity monitoring method using the same.SOLUTION: An ecotoxicity monitoring device using a luminescent bacterium and an ecotoxicity monitoring method using the same are provided. The ecotoxicity monitoring device comprises: a reaction tank module including a reference reaction tank, a sample reaction tank, and a dilution reaction tank, and supplies a mixed solution containing the luminescent bacterium; a luminescent bacterium culture portion; a diluted water supply portion; a sample supply portion; an optical measurement portion that measures the amount of luminescence of the luminescent bacterium in a toxicity assessment substance provided from the sample reaction tank and the dilution reaction tank; a control portion; and an analysis portion that determines whether a toxic substance is present a sample on the basis of a light quantity value provided from the optical measurement portion. After time predetermined as an initial light quantity value of the reference reaction tank measured through the optical measurement portion has elapsed, the analysis portion is configured to calculate a correction coefficient through the measured final light quantity value, and calculate a corrected light quantity value of the sample reaction tank and dilution reaction tank through the calculated correction coefficient.SELECTED DRAWING: Figure 1

The application discloses a kind of lactic acid bacteria PH inhibition method water quality online biological toxicity detection method and detection device, wherein detection method includes the following steps: preparation first water sample and second water sample;Get the initial PH voltage value of first water sample and second water sample;Constant-temperature culture is carried out to first water sample and second water sample;Respectively obtain the PH voltage value of first water sample and second water sample after cultivation;Biological toxicity analysis calculation is carried out to water sample to be measured.The method of the application determines the size of harmful substances in water by monitoring the PH change caused by lactic acid, a metabolic product of lactic acid bacteria;Based on the mature glass electrode electrochemical principle method to detect PH, its detection accuracy is not affected by water quality turbidity;The detection method of the application has high consistency with the luminescent bacteria method of prior art for detecting biological toxicity, and meets the related technical requirements in the determination of water quality acute toxicity.

The invention discloses an organism self-repairing monitoring method based on bioluminescence recovery after photobleaching, a monitoring device and application. Luminescent bacteria are used as model organisms, light damage is induced, luminous intensity recovery kinetics are monitored in real time, and quantitative analysis of the repair activity is achieved in combination with quantitative parameters (initial luminous intensity, bleaching degree, recovery degree, recovery time and initial repair rate). According to the method, bioluminescence driven by metabolism of an organism is utilized, exogenous fluorescence labeling is not needed, and light damage interference in the monitoring process is avoided; and the luminescence recovery process after photobleaching is dynamically monitored, so that the hysteresis and discontinuity of the traditional method are avoided. The method can be used for screening, promoting or inhibiting biological self-repairing medicine development, repairing mechanism research and disease repairing research.

To provide an ecotoxicity monitoring device using a luminescent bacterium and an ecotoxicity monitoring method using the same.SOLUTION: An ecotoxicity monitoring device using a luminescent bacterium and an ecotoxicity monitoring method using the same are provided. The ecotoxicity monitoring device comprises: a reaction tank module including a reference reaction tank, a sample reaction tank, and a dilution reaction tank, and supplies a mixed solution containing the luminescent bacterium; a luminescent bacterium culture portion; a diluted water supply portion; a sample supply portion; an optical measurement portion that measures the amount of luminescence of the luminescent bacterium in a toxicity assessment substance provided from the sample reaction tank and the dilution reaction tank; a control portion; and an analysis portion that determines whether a toxic substance is present a sample on the basis of a light quantity value provided from the optical measurement portion. After time predetermined as an initial light quantity value of the reference reaction tank measured through the optical measurement portion has elapsed, the analysis portion is configured to calculate a correction coefficient through the measured final light quantity value, and calculate a corrected light quantity value of the sample reaction tank and dilution reaction tank through the calculated correction coefficient.SELECTED DRAWING: Figure 1

This invention relates to the field of water pollution detection technology, specifically to a method for preparing quantum dot fluorescent probes for online monitoring of comprehensive water toxicity. The method uses L-histidine and levofloxacin as raw materials, and through hydrothermal reaction, centrifugation, dialysis, pH adjustment, filtration, and freeze-drying, prepares histidine-modified carbon quantum dot fluorescent probes (His-CQDs), which are further formulated into a working solution. The histidine is used to simulate key toxicity recognition sites in the luciferase system of luminescent bacteria, enabling the His-CQDs to produce fluorescence quenching responses to heavy metal ions and fluorescence enhancement responses to organic pollutants, thereby achieving online monitoring of the comprehensive toxicity of the water sample. During detection, the working solution is mixed with the water sample, and the fluorescence intensity is measured. The water toxicity evaluation result is obtained by calculating the fluorescence change rate. This invention achieves a materialized simulation of the toxicity recognition mechanism of luminescent bacteria, and has the advantages of fast response, good stability, and suitability for online monitoring of comprehensive water toxicity.

According to the marine overboard personnel positioning sheet and positioning system based on the photogenic bacteria, escherichia coli is adopted as a chassis organism, a luxABCDEFG gene and an IPTG inducible promoter are inserted, and controllable luminescence is achieved; and culturing the strain, freeze-drying, packaging the strain, IPTG powder and nutritional ingredient powder in a 500-1000 Da dialysis bag, reviving when meeting water, and emitting light. Experimental results show that the luminescence time of the escherichia coli containing the luciferase report system gene is greater than or equal to 6 hours; the freeze-dried bacterial powder can be stored at normal temperature for more than or equal to 6 months, and the wavelength of emitted blue light is 480-520 nm, so that the problems of weak positioning signal, short duration and easiness in confusion with ambient light of the overboard personnel at night are solved.