Method for detecting acute toxicity of copper-contaminated soil using freshwater luminescent bacteria

A technology of luminescent bacteria and acute toxicity, which is applied in the field of detecting acute toxicity of copper-contaminated soil with freshwater luminescent bacteria, can solve the problems of complex matrix, affecting soil toxicity test results, changing sample background conditions, etc., and achieves simple operation, safe use, and method novel effect

Inactive Publication Date: 2011-12-21
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0003] However, when applied in soil samples, in order to maintain a certain osmotic pressure environment, 3.5% NaCl solution is added to the sample, which significantly changes the background conditions of the sample, thus affecting the toxicity test results of the soil.
Moreover, the matrix of soil samples is relatively complex, and the application of luminescent bacteria to the toxicity detection of soil samples is affected by many factors. Therefore, there are still many problems and difficulties in the application of luminescent bacteria to the monitoring of solid-phase pollutants such as soil.

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  • Method for detecting acute toxicity of copper-contaminated soil using freshwater luminescent bacteria
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  • Method for detecting acute toxicity of copper-contaminated soil using freshwater luminescent bacteria

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[0025] Describe in detail below in conjunction with the embodiment of the present invention: this embodiment implements under the premise of the technical solution of the present invention, has provided detailed implementation and specific operation process, but protection scope of the present invention is not limited to the following Example.

[0026] Qinghai Vibrio Q67 (Vibrio-qinghaiensis sp.-Q67) is a freshwater luminous bacterium isolated from the surface of Qinghai naked carp (Gymnocypris przewalskii) in Qinghai Lake, my country. The bacterium has natural luminescent properties and is not pathogenic sex. Therefore, the application of Qinghai Vibrio Q67 to the acute toxicity detection of copper-contaminated soil samples has a greater advantage than that of seawater luminescent bacteria.

[0027] The present invention is achieved through the following technical solutions, comprising the following steps:

[0028] The first step, preparation of luminescent bacteria suspensi...

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Abstract

The invention relates to a detection method in the technical field of environmental monitoring, in particular to a method for detecting the acute toxicity of copper-contaminated soil by using freshwater luminescent bacteria, which overcomes the limitations of the application of seawater luminescent bacteria in the detection of acute toxicity of soil samples. The specific method selects freshwater luminescent bacteria as the bacteria for detection, including the following steps: the first step: prepare the bacterial suspension of freshwater luminescent bacteria with the soil background solution; the second step, extract the soil pore water by double-chamber centrifugation; the third step, Testing soil samples for toxicity.

Description

technical field [0001] The invention relates to a detection method in the technical field of environmental monitoring, in particular to a method for detecting the acute toxicity of copper-contaminated soil by using freshwater luminescent bacteria. Background technique [0002] When luminescent bacteria come into contact with toxic and harmful substances, the luminous intensity is weakened, and the degree of luminous intensity weakening has a certain correlation with the content of toxic substances. Using this characteristic of luminescent bacteria, it is used as an indicator microorganism, and the change of luminous intensity is used as an indicator to detect the biological toxicity of toxic and harmful substances in the environment, so as to monitor the pollutants in the environment and evaluate the pollution risk. Luminescent bacteria such as seawater luminescent bacteria Vibrio fischer have been studied and applied in the toxicity detection of samples in water environment...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/01G01N1/34G01N21/76
Inventor 韦东普马义兵
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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