6 results about "Cholinergic cells" patented technology

This invention relates to the field of organic small molecule fluorescent probe technology, specifically to a neuron-specific dual-targeting fluorescent probe targeting the AChR of cholinergic neurons and its applications. The fluorescent probe, whose structure follows general formula I, is characterized by its dual-targeting structure, making it particularly suitable for the identification and labeling of cholinergic neurons. Furthermore, this fluorescent probe exhibits significant advantages in multiple application scenarios, such as cell imaging, protein and organelle labeling, tumor-specific recognition, and photodynamic therapy, with its potential application value in neuroimaging being particularly prominent. This fluorescent probe is expected to advance the development of neurobiology, disease diagnosis, and treatment methods, and has promising application prospects.

Disclosed herein are methods of treating cancers of the central nervous system (e.g. CNS tumors) in a subject involving providing to the subject a muscarinic acetylcholine receptor M1 and / or a muscarinic acetylcholine receptor M3 antagonist to the subject. Further disclosed herein are co-cultures comprising cholinergic neurons and glioma cells that may be used for therapy screening / testing, and methods of producing the same.

It has been found that the presence of cholinergic T cells is important for an effective immune response against hepatocellular carcinoma (HCC). These results demonstrate a new aspect of modulating cancer immune surveillance by immune cell-derived neurotransmitters and elucidate their effect in supporting cells to produce anti-tumor responses. Methods of treating HCC in a subject in need thereof are disclosed. Generally, the methods involve treating a subject with a therapy effective to increase acetylcholine levels in T cells. Also disclosed are methods in which a subject is treated with an anti-PD-1 immunotherapy when the determined cholinergic T cell level in the subject is high, or a subject is prevented from being treated with an anti-PD-1 immunotherapy when the determined cholinergic T cell level in the subject is low.

The invention discloses an MXene / enzyme modified acetylcholine nano microelectrode and application of the MXene / enzyme modified acetylcholine nano microelectrode in analysis of acetylcholine released by a single vesicle. The MXene / enzyme modified acetylcholine nano microelectrode constructed by the invention is used for analysis of acetylcholine released by the single vesicle. The method sequentially comprises the following steps: construction of an acetylcholine nano microelectrode, preparation of an acetylcholine artificial vesicle, extraction and culture of mouse primary cholinergic neurons, and release detection of acetylcholine in a single vesicle. A new thought and a new method are provided for monitoring the release condition of the acetylcholine single vesicles at the single cell level, and the method has important significance in studying nervous system development, understanding the physiological and pathological processes of nervous system diseases and regulating rules in treatment.

Methods for electrical modulation of inflammation or serum TNF levels in a subject.

This invention provides an oligosaccharide composition for improving neurodevelopment and functional disorders, comprising 6'-sialyl lactose and lactose-N-trisaccharide. The oligosaccharide composition provided by this invention can effectively inhibit the accumulation of Aβ protein, which leads to neuronal synaptic disorders, neuronal apoptosis, brain damage, and functional disorders. Therefore, the oligosaccharide composition provided by this invention can protect nerves and improve functional disorders. Furthermore, the oligosaccharide composition provided by this invention can also inhibit acetylcholinesterase activity, protect the normal development of cholinergic neurons, and increase neurotransmitter levels, thus benefiting the improvement of neurodevelopment, learning and memory abilities, and the improvement of behavioral coordination in infants and young children.