Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing animal brain injury model based on hippocampal cholinergic neuron regulation and control

A cholinergic neuron and injury model technology, applied in the field of medicine, can solve the problems of lack of construction and evaluation of electromagnetic radiation brain injury animal models, lack of prevention and treatment measures, etc.

Active Publication Date: 2022-07-08
ACADEMY OF MILITARY MEDICAL SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Because the molecular mechanism of the electromagnetic radiation brain damage effect has not yet been elucidated, the target cell types and their molecular targets sensitive to electromagnetic radiation are also unclear, so far there is a lack of good construction and Methods for evaluating animal models of electromagnetic radiation brain injury, and related prevention and treatment drugs are lacking, so it is urgent to design a technical solution that can overcome the above defects to a certain extent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing animal brain injury model based on hippocampal cholinergic neuron regulation and control
  • Method for constructing animal brain injury model based on hippocampal cholinergic neuron regulation and control
  • Method for constructing animal brain injury model based on hippocampal cholinergic neuron regulation and control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Materials and methods

[0037] 1.1Chat-iCre mouse construction

[0038] Choline acetyltransferase (ChAT), a marker gene of cholinergic neurons, was used as the target gene, and P2A-iCre-P2A-tdTomato was inserted before the gene stop codon. The homology arms at the 5' and 3' ends are approximately 2 kb, respectively. Knock-in mice were prepared using the EGE system developed by Biositu based on CRISPR / Cas9. The schematic diagram of the targeting vector is as follows figure 1 shown.

[0039] 1.2Rosa26-ChR2 tool mouse construction

[0040] According to the characteristics of the mouse Rosa26 gene, CAG-loxP-Stop-loxP-ChR2-EGFP-WPRE-pA was inserted at the Rosa26 site. Gene knock-in mice were prepared using the EGE system developed by Biositu based on CRISPR / Cas9. The schematic diagram of the targeting vector is as follows figure 2 shown.

[0041] 1.3 Brain Stereotactic Injection of AAV and Fiber Optic Implantation

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing an animal brain injury model based on hippocampal cholinergic neuron regulation, which comprises the following steps: setting electromagnetic radiation conditions, and carrying out electromagnetic radiation treatment on a test animal; and comparing the influence of cholinergic neuron activation or inhibition of the hippocampal CA3 region of the experimental animal on the behavior of the experimental animal before and after the electromagnetic radiation treatment, and if the influence on the behavior of the experimental animal has statistical significance, constructing the electromagnetic radiation animal brain injury model by utilizing the electromagnetic radiation condition. According to the method, the electromagnetic radiation animal brain injury model can be well constructed, and possibility is increased for clarification of the molecular mechanism of the electromagnetic radiation brain injury effect and research and development of prevention and treatment drugs.

Description

technical field [0001] The present invention relates to the technical field of medicine. More specifically, the present invention relates to a method for constructing an animal brain injury model based on the regulation of hippocampal cholinergic neurons. Background technique [0002] Existing studies have found that electromagnetic radiation can cause changes in the morphological structure of brain tissue, including hippocampus, cerebral cortex, cerebellum, amygdala, and blood-brain barrier. Among them, the hippocampus is a key brain area involved in learning and memory functions. It is not only a long-term It is also involved in the formation of short-term memory and spatial memory, and is also involved in the regulation of emotional behavior. Our previous study found that the pathological changes of the hippocampus caused by microwave radiation were more obvious in the CA3 area. Using gene chip and proteomics technology, it was found that cytoskeleton damage, energy met...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K67/027
CPCA01K67/0275A01K67/0278A01K2227/105A01K2267/03A01K2217/206
Inventor 左红艳李杨郝延辉胡翠翠叶雨萌常晨旭孔晓旭鲁艳杰冯志华
Owner ACADEMY OF MILITARY MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com