Luminescent bacteria test paper for rapidly detecting water quality biotoxicity and preparation method thereof

A technology of luminescent bacteria and water organisms, which is applied in the fields of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, etc.

Inactive Publication Date: 2013-05-15
FUDAN UNIV
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Problems solved by technology

[0007] However, there is no report on the immobilization of l

Method used

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Embodiment Construction

[0023] Test strip preparation. Dissolve the freeze-dried powder of Luminescent bacteria in hydrogel (95% PEGDA and 5% HMPP), and dissolve it in 100 μL of hydrogel according to the amount used for one test (for example: as in the traditional experiment, this tube of freeze-dried powder is used for For three tests, mix with 300 μL of hydrogel when preparing test strips). Take 100 μL of the mixture of hydrogel and bacterial freeze-dried powder and drop it on the test paper, with an area of ​​about 1×1cm 2 , Put the test paper under the ultraviolet light for about 10s, the hydrogel will solidify, and store it in the refrigerator at -20oC until use.

[0024] Bacteria powder recovery. When water quality biotoxicity testing is required, put the test paper immobilized with hydrogel into 2% NaCl solution (at least 1mL) and resuscitate the bacteria powder at room temperature, and the recovery can be completed in 15 minutes. Upon dissolution, the luminescent bacteria were also reactiv...

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Abstract

The invention belongs to the technical field of biological analysis and detection and in particular relates to luminescent bacteria test paper for rapidly detecting water quality biotoxicity and a preparation method thereof. The luminescent bacteria test paper is prepared by the following steps: taking a common test paper material as a substrate, coating luminescent bacteria through hydrogel, solidifying the coating luminescent bacteria from a liquid state to a solid state under the irradiation of an ultraviolet lamp, and fixing the solid luminescent bacteria on the test paper. The test paper is thrown into a water body to be detected, the initial luminous amount value of the luminescent bacteria and the luminous amount value of the luminescent bacteria (in the water body) within 15 minutes or 30 minutes on the test paper are compared so as to obtain the light-inhibiting rate, so that the water quality biotoxicity is detected. The method has the characteristics of rapidness, high efficiency, convenience, low cost and the like, also has high sensitivity and high tolerance to external condition change and is suitable for rapid on-site toxicity detection in various water environments.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, and in particular relates to a luminescent bacteria test paper capable of rapidly detecting water biological toxicity on the spot, and provides a preparation method of the test paper. Background technique [0002] With the increasing degree of industrialization and the sudden occurrence of various chemical products polluting water bodies, the number of various chemical substances has increased sharply, which has greatly affected the living environment of human beings. It is urgent to identify the acute toxicity of the increasing environmental pollutants. Traditional chemical analysis methods can accurately and quantitatively analyze the content of main components in pollutants, but cannot directly and timely reflect the comprehensive impact of various toxic substances on the environment and organisms. Traditional animal toxicity experiments use mammals, fish, etc. as sub...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 隋国栋张金玲聂晓冬刘思秀
Owner FUDAN UNIV
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