94 results about "Cryoprotective Agent" patented technology

The present invention relates to methods for cryopreserving plant cells and to methods for recovering viable plant cells from long or short term cryopreservation. Plant cells to be cryopreserved can be grown in culture and pretreated with a solution containing an cryoprotective agent and, optionally, a stabilizer. Stabilizers are preferably membrane stabilizers such as ethylene inhibitors, oxygen radical scavengers and divalent cations. Cells can also be stabilized by subjecting the culture to a heat shock. Pretreated cells are acclimated to a reduced temperature and loaded with a cryoprotective agent such as DMSO, propylene glycol or polyethylene glycol. Loaded cells are incubated with a vitrification solution which, for example, comprises a solution with a high concentration of the cryoprotective agent. Vitrified cells retain less than about 20% water content and can be frozen at cryopreservation temperatures for long periods of time without significantly altering the genotypic or phenotypic character of the cells. Plant cells may also be cryopreserved by lyophilizing cells prior to exposure to a vitrification solution. The combination of lyophilization and vitrification removes about 80% to about 95% of the plant cell's water. Cells can be successfully cryopreserved for long periods of time and viably recovered. The invention also relates to methods for the recovery of viable plant cells from cryopreservation. Cells are thawed to about room temperature and incubated in medium containing a cryoprotective agent and a stabilizer. The cryoprotective agent is removed and the cells successfully incubated and recovered in liquid or semi-solid growth medium. The invention also relates to the cryopreserved cells and to viable plant cells which have been recovered from long or short term cryopreservation.

The invention provides a cell freezing medium. The cell freezing medium comprises acylated epsilon polylysine, polyhydric alcohols and equilibration buffer. According to the cell freezing medium disclosed by the invention, the polyhydric alcohols and acylated epsilon polylysine replace toxic dimethyl sulfoxide so as to serve as cryoprotective agents, so that damage to cells in the freezing resuscitation process is reduced, and the cell survival rate is improved. The invention further provides a method for performing freezing resuscitation on cells by utilizing the cell freezing medium and application of the method for freezing stem cells and immune cells. The method is simple and feasible to operate and is suitable for large-scale clinical application of cell therapy, and great convenienceis brought to clinical application of the cell freezing technology.

The invention discloses a compound plant anti-freezing agent and a preparation method thereof, aiming at the problems of single function and short protection period of the traditional plant anti-freezing agent. The compound plant anti-freezing agent comprises the following ingredients: 8-12% of a film-forming agent, 4-6% of saccharide, 10-16% of an anti-freezing reagent, 0.8-1.6% of an anti-freezing element, 4-6% of a micro element fertilizer, 0.2-0.4% of vitamin, 0-2% of a growth regulating agent, 1.2-2% of a surfactant and 60% of magnetized water. The compound plant anti-freezing agent is characterized in that the magnetized water is used for replacing common water to improve the activity of the anti-freezing agent. On one hand, one layer of protective film can be formed on the surface of the plant so as to perform the functions of resisting cold, preventing freezing and reducing transpiration water loss. On the other hand, the content of saccharide in plant cells can be improved, the activity of plasma membrane ATP (adenosine tTriphosphate) enzyme is increased, the phenomena of freezing in cells and cell freezing dehydration are avoided, and therefore the influence on the plant by freezing injury is effectively lowered.

A cryoprotective composition which comprises nanostructures, liquid and at least one cryoprotective agent is provided.

The invention discloses an Atrac tylodes macrocephala koidz conservation method and re-cultivating method. The conservation method comprises the following steps: (1) taking stem tips of Atrac tylodes macrocephala koidz tissue culture seedlings, and placing the stem tips in a pre-culture solution which is an MS (Murashige and Skoog) fluid nutrient medium containing 0.3 mol/L cane sugar for pre-culture; (2) taking the stem passing through from the step (1) and pre-processing the stem tips in a pre-processing solution which is an MS fluid mutrient medium containing both 2.0 mol/L glycerol and 0.4 mol/L cane sugar; (3) taking the stem tips passing through the step (2) and processing the stem tips in a cryoprotective agent which is prepared in the way that 3.26 mol glycerol, 2.42 mol ethylene glycol, 1.9 mol dimethyl sulfoxide, 0.4 mol cane sugar and the MS fluid nutrient medium are uniformly mixed to 1 L; and (4) putting the stem tips passing through the step (3) into liquid nitrogen for conservation. The Atrac tylodes macrocephala koidz conservation method is low in cost, simple and convenient in operation and high in plant regeneration rate, and is an effective way in vitro conservation of Atrac tylodes macrocephala koidz germplasm resources.

The invention relates to the technical field of processing of aquatic products, and in particular relates to a fresh shrimp freezing method and a fresh shrimp cryoprotective agent. The cryoprotective agent is composed of an agent A and an agent B, wherein the agent A is bamboo vinegar; the agent B is a mixture of saccharose, tea polyphenol, nisin, trehalose, glycerinum, mannitol and chitosan; in addition, in terms of 100% of the agent B, various components are as follows: 2-3% of saccharose, 4-6% of tea polyphenol, 0.5-1% of nisin, 12-20% of glycerinum, 20-22% of mannitol, 12-15% of chitosan, and the balance of trehalose. The use sequence of the fresh shrimp cryoprotective agent comprises processing by using the agent A at first and then processing by using the agent B to coat a film; the sequence cannot be changed; by virtue of adopting the method, nutrition of unfrozen fresh shrimp is low in loss; meanwhile delicate flavour of fresh shrimp is also well protected; the original appearance condition is kept.

The invention relates to a fruit tree disease and freeze preventing agent, which belongs to a preparation for preventing freeze and diseases of fruit trees. The fruit tree disease and freeze preventing agent mainly comprises the following components in percentage by weight: 5% of agar, 5% of glycerol, 15% of sucrose, 5% of plant growth regulator and 70% of water. The fruit tree disease and freeze preventing agent is prepared by a method comprising the following steps: firstly immersing the agar for two hours with small amount of warm water on the hand; heating the agar to melt the agar melt; adding other components; and evenly stirring the mixture so as to obtain the fruit tree disease and freeze preventing agent. The raw material adopted by the fruit tree disease and freeze preventing agent disclosed by the invention has the advantages of strong penetration, inner absorption, high efficiency, broad spectrum, low toxicity and low manufacturing cost, is safe and is convenient to use.

The invention discloses a cryoprotective agent for maintaining the high killing ability of DC-CIK (dendrite cell-cytokine induced killer) cells. The cryoprotective agent comprises RPMI 1640, fetal calf serum, dimethyl sulfoxide and heterophyllin B or C. The RPMI 1640, the fetal calf serum and the dimethyl sulfoxide are mixed with one another according to a volume ratio of 6:3:1; the concentration of the heterophyllin B is 7-9 micro-g/ml; the concentration of the heterophyllin C is 4-6 micro-g/ml. The cryoprotective agent has the advantages that the high killing ability of the DC-CIK cells can be maintained to the greatest extent on the basis that the cryopreservation resuscitation rates of the DC-CIK cells are guaranteed, and the strong killing ability owing to low expression of miRNA [micro-RNA (ribonucleic acid)]-155 can be lengthened.

The invention discloses xenogeneic acellular dermis and a preparation method thereof. The preparation method comprises steps of acquiring and preserving a pig dermal slice and conducting acellular treatment, wherein the pig dermal slice is preserved with the addition of a cryoprotective agent, and for the acellular treatment, a mild detergent is adopted to break cells. According to the xenogeneic acellular dermis and the preparation method thereof provided by the invention, freeze-thaw cycles are avoided, and by adding the cryoprotective agent in a freezing process, damage to the bio-activity of collagen is relieved and the rapid recovery of a wound surface is promoted; residual ribonucleic acid is effectively removed by virtue of a ribonuclease solution, so that an adverse reaction caused by the residual ribonucleic acid is avoided; [alpha]-galactose is effectively removed by virtue of an [alpha]-galactosidase solution, so that an acute immune response in human body caused by the [alpha]-galactose is relieved and even avoided; by conducting electron beam end sterilization in an anti-radiation protective agent or by conducting [gamma] radiation under a dry ice condition, damage of the radiation to the pig dermal slice is reduced while conducting the sterilization; and the xenogeneic acellular dermis, which is packaged in an aluminum foil inside and outside dual-layer bag, can be conveniently opened and used by a doctor.

The invention discloses quick luminescent bacteria supported based on signal molecule. The quick luminescent bacteria supported based on signal molecule are characterized in that: luminescent bacteria freeze-dried powder is prepared by mixing bacterial mud obtained by centrifuging 10ml of Vibrio fischeri at the concentration of 10<9>/ml and 1ml of cryoprotective agent, and performing freeze drying, and is stored at -20DEG C for 6 months; and the cryoprotective agent is prepared by dissolving 15g of nonfat milk, 5g of glycerol and 12.5g of trehalose in 100ml of signal molecule solution. The invention has the advantages that: by adding signal molecules, the synthesis of luciferase can be promoted, so that the time for the luminescent bacteria freeze-dried powder to restore stable luminescence is shortened from the original 15-30 minutes to 5 minutes; and by adding the composite cryoprotective agent, more bacteria can keep the integrity of cellular structures and the activity of cells during freezing and drying, and can be revived under proper conditions.

The present disclosure is directed to the use of a cryoprotective agent to protect healthy tissue while damaged tissue is cryosurgically treated. A cryoprotective agent can be delivered utilizing a delivery probe that is inserted into healthy tissue either prior to or during a freeze portion of a first freeze/thaw cycle. The cryoprotective agent diffuses through the healthy tissue, but diffusion is controlled at the freeze edge by the diffusion limiting characteristics of the tissue that is frozen. When the frozen tissue is thawed, the disrupted tissue will continue to restrict the diffusion of the cryoprotective agent. Additional freeze/thaw cycles can then be conducted and the cryoprotective agent will continue to protect the healthy tissue. The delivery probes can also function as thermal sensor probes that can also be used to monitor the temperature at selected tissue locations.

The invention discloses a production method of a water-based capillary crystalline waterproof material convenient to produce and use. An additive comprises the following components: 30 parts of a gelmaterial, 16.7 parts of a crystallization accelerator, 0.12 part of an additive, 3 parts of a cement molecule secondary reactant, 0.07 part of an anti-freezing agent, 0.15 part of a metal ion complexing agent, 0.4 part of a curing agent, 1 part of a hydrophobic agent and 48.56 parts of a diluent. The materials of the components are subjected to burdening, stirring, mixing and filtering to obtain the water-based capillary crystalline waterproof material. The invention discloses a production method of a water-based capillary crystalline waterproof material convenient to produce and use. Jelly-shaped crystals and sulphoaluminate, silicate and the like participating in the reaction thoroughly form crystals under the action of sodium fluosilicate; in addition, in order to ensure that enough waterproof agents permeate into a concrete base layer, the reaction speed needs to be controlled, flocculent crystals are prevented from appearing too early, so that a metal ion complexing agent needs tobe used for delaying the reaction speed.

The invention discloses a water-absorption-bead cold-and-hot-compression physical therapy bag. The water-absorption-bead cold-and-hot-compression physical therapy bag comprises energy-storage water-absorption-bead inner materials and an outer bag. The energy-storage water-absorption-bead inner materials are installed inside the outer bag, and are prepared from, by weight, 3-30 parts of water-absorption beads, 0.1-50 parts of anti-freezing agent, 0.1-5 parts of lubricating agent, 0.1-10 parts of toughness adjusting agent, 0.05-2 parts of preservative and 10-60 parts of water. The invention discloses a production method of the water-absorption-bead cold-and-hot-compression physical therapy bag at the same time. The water-absorption-bead cold-and-hot-compression physical therapy bag and the production method thereof have the advantages that water-absorption beads mixed in the product have the characteristics of being high in transparency, crystal clear, attractive in form, extremely good in elasticity, excellent in water locking performance and the like, the potential problem that after external packing of a common gel-type product is fractured, liquid is leaked is solved, the good elasticity and the good lubrication degree can be kept after low-temperature storage or a high temperature water bath or microwave heating, and the cold and hot performance can be kept for a long time; meanwhile, the bag is easy to carry and free of transformation, and the problems that after the inner materials are repeatedly used, the viscosity of the inner materials is reduced, and water losing hardening occurs are solved.

The invention discloses an anti-corrosive outer casing of an electric pump. The outer casing of the electric pump is coated with an anti-corrosive protecting layer; the anti-corrosive protecting layeris formed by compounding a component A and a component B according to a ratio of 1:3; the component A is prepared from the following components of 8 to 11 parts of poly(aryl ether sulfone ketone) modified resin, 22 to 29 parts of silicon carbide micropowder, 10 to 15 parts of fluorinated graphite, 21 to 24 parts of black silicon carbide, 18 to 24 parts of benzoyl peroxide, 20 to 25 parts of aminoresin, 5 to 9 parts of neopentyl glycol, 11 to 19 parts of sodium pyrophosphate, 13 to 19 parts of base emulsion, 12 to 18 parts of anti-freezing agent, 22 to 28 parts of filler, and 34 to 36 parts of auxiliary materials; the component B is prepared from the following components of 8 to 13 parts of talcum powder, 8 to 14 parts of bisphenol F type liquid epoxy resin, 13 to 19 parts of calcium chloride, 3 to 9 parts of quartz, 15 to 20 parts of butyl acetate, 2 to 9 parts of 2-butanone cyclohexanone, 16 to 22 parts of plasticizing resin, 13 to 19 parts of fused corrosion inhibitor, 20 to 22 parts of curing agent, 21 to 25 parts of defoamer, 12 to 19 parts of auxiliary material, and 20 to 22 parts of solvent.

The invention provides a micro-device for removing a cell cryoprotective agent on the basis of a multistage dialysis method. The micro-device comprises an upper chip with a first channel, a lower chip with a second channel, and a porous membrane, wherein the porous membrane is arranged between the dialysis units of the upper chip and the dialysis units of the lower chip; the first channel comprises a cell suspension channel and four dialysis units; the second channel comprises a dialysis liquid generating area, four dialysis units and a dialysis liquid collecting area; the dialysis units of the first channel, the dialysis units of the second channel and the porous membrane form a dialysis executing area. When the micro-device operates, the dialysis liquid generating area of the second channel generates gradient-concentration and variable-flow dialysis liquid, and the dialysis liquid flows into the dialysis executing area to dialyze the cell suspension in the dialysis units of the first channel. The micro-device has the advantages that the micro-device can effectively remove the cryoprotective agent in the cell suspension, reduce cell mechanical damage and osmotic pressure damage, and increase cell survival rate and recycling rate.

The invention discloses an application of sedge quinone and analogues thereof in culturing NK cells. The invention finds that sedge quinone and analogues thereof, including demethylcyperapuinone and hydroxycyperone, have various pharmacological activities, such as, capability of serving as cryoprotective agents for CIK cells and NK cells, capability of promoting the in vitro multiplication of CIKcells and NK cells and capability of promoting the adipose-derived stem cell osteoblast differentiation. The invention also provides single-open loop derivatives and double-open loop derivatives of the sedge quinone and analogues thereof and a preparation method thereof. The single-open loop derivatives and double-open loop derivatives of the sedge quinone and analogues thereof can promote the invitro multiplication of CIK cells. A searching result shows that the applications of the sedge quinone and analogues thereof in promoting the multiplication of CIK cells and NK cells and promoting thestem cell osteoblast differentiation are not disclosed in the prior art and the single furan ring and double furan ring open loop derivatives of the sedge quinone and analogues thereof are also not reported.

The invention relates to a plant food material comprising at least one cryoprotective agent present in the intracellular space of the cells of said plant. The invention also relates to a freezing method for a plant food product comprising the steps of; providing a plant food material comprising at least 70% water, applying a pulsed electrical field to said plant food material, adding a cryoprotective agent to said plant food material, applying a pressure to said plant food material followed by, a resting period of at least 30 minutes and freezing said plant food material. Thereby it is for the first time possible to conserve a plant food material and maintaining the texture as well as the taste of the plant food material

The invention relates to a tea tree anti-freezing agent and a preparation method thereof and belongs to the technical field of tea tree anti-freezing. The tea tree anti-freezing agent comprises the following raw materials according to weight ratio: 0.05 to 0.15 percent of paclobutrazol, 0.02 to 0.05 percent of sodium benzoate, 5.0 to 10.0 percent of mineral oil, 3.0 to 5.0 percent of ethyl alcohol and water in balancing amount. The tea tree anti-freezing agent provided by the invention has the following benefits: 1, the tea tree anti-freezing agent is nontoxic, harmless and has no pollution to environment, can be directly sprayed onto plant leaf surfaces, and is simple and convenient to use, the preparation method of the tea tree anti-freezing agent is simple, and the effective freeze-proof period can reach 7 to 10 days. 2, the tea tree anti-freezing agent can activate biological enzyme, prevent the formation of the ice nucleating bacteria, induce the production of anti-freezing factors, and can improve the resistivity of plants to frozen injury. 3, the tea tree anti-freezing agent provided by the invention can form a protecting film on the surface of a tea tree, and has the capability of protecting plants from resisting coldness.

The invention relates to the technical field of plant anti-freezing agents, and more specifically, relates to a plant anti-freezing agent containing organic matters and a preparation method thereof. The anti-freezing agent comprises organic matters such as sugar containing matters, plant active matters, functional matters, and the like. The raw materials are all organic matters, and are nontoxic and pollution-free. The anti-freezing agent can be used in any phase of the growth period of plants, has an anti-freezing function, can provide nutrients to plants, can effectively activate the activity of plants, and thus improves the resistance of plants. The adhesion performance of the anti-freezing agent is strong, so the ant-freezing agent is difficult to remove by rainwater. Moreover, the dissolving performance and permeability of the ant-freezing agent are good. After the anti-freezing agent enters plants, the anti-freezing agent can form a protective film on the inner surface of plants, the size of the pores of leaves is reduced on the premise that the breath of plants is not influenced, thus the water loss caused by transpiration is reduced, drought stress caused by low temperature stress is prevented; the freezing point of water on the leaf surface is reduced, the cell sap concentration is increased, metabolism of cells is regulated, and the damage of plants caused by cold environment is relieved.

The invention discloses an ovary vitrification cryopreservation method under intervention of luteinizing hormone. The ovary vitrification cryopreservation method comprises the following steps: soaking an in-vitro ovary into a culture solution, putting into a constant-temperature incubator with 5 percent of CO2 at the temperature of 37 DEG C for culture, then transferring into a permeable cryoprotective agent-containing ethylene glycol pre-equilibrium solution for soaking, after then, transferring into a high-concentration cryoprotective agent-containing vitrified cryopreservation fluid for continuous permeable equilibrium, and finally transferring into liquid nitrogen for soaking for at least 24 hours. A thawing process comprises the steps of taking out the cryopreserved ovary from the liquid nitrogen, placing the ovary into 0.50 mol/L, 0.25 mol/L and 0.125 mol/L of thawing solutions which are preheated at the temperature of 37 DEG C and has the sucrose concentration gradually decreased in sequence for 10 min, then transferring the ovary into the culture solution, and putting into the constant-temperature incubator with 5 percent of CO2 at the temperature of 37 DEG C for culture; the culture solution, the pre-equilibrium solution, the vitrified cryopreservation fluid and the thawing solution contain the luteinizing hormone, so that the survival rate of the cryopreserved ovary can be increased, and the reproductive function and the endocrine function can be retained to a relatively large extent.

The invention relates to the technical field of antifreezing agents and in particular relates to a plant antifreezing agent. The plant antifreezing agent is prepared from the following raw materials in parts by weight: 10-30 parts of tea saponin, 1-7 parts of calcium chloride, 8-18 parts of urea, 4 parts of sodium naphthalene acetate and 2-9 parts of cerous nitrate. The plant antifreezing agent provided by the invention is significant in antifreezing effect, has an effective antifreezing period of 12-20 days, can be directly sprayed to plants, is simple and convenient to use, and can prevent freezing and improve the cold resistance, certain nutrients of the antifreezing agent on the surface of a fruit tree are absorbed, and the antifreezing agent falling on the ground can be used as a fertilizer mixed into soil and absorbed by roots to promote the growth of the fruit tree, so that the antifreezing agent is fully utilized and wastes are prevented.

The present invention is a method of freezing cells comprising the steps of incubating said cells in a solution comprising a cryoprotective agent, concentrating the cells resulting from the previous step withdrawing the eluent essentially free of cells, and freezing the resulting concentrated cells. The cells frozen by the invention's method render a high post-thawing viability, reduce cryoprotectant related toxic events and promote cells life in a suspension state after thawing. The invention also comprises the container comprising the frozen cells.

A new type of cryoprotective agents that are useful for retaining the viability and metabolic activity of frozen or freeze-dried microbial cultures, is disclosed. The cryoprotective agent comprises compounds involved in biosynthesis of nucleic acids. Methods for the preparation as well as the uses of such cultures are given. Such cultures are useful as starter cultures in the manufacturing of food and feed products. Starter cultures of the invention include culture of lactic acid bacteria, e.g. Lactococcus species as well as other species.