Quick luminescent bacteria supported based on signal molecule

A technology of signaling molecules and luminescent bacteria, which is applied in the field of bacterial agents to detect water quality toxicity, can solve the problems of inability to realize online monitoring of water quality, poor luminescent stability of freeze-dried powder, and affecting the accuracy of toxicity monitoring.

Inactive Publication Date: 2011-11-23
CHONGQING UNIV
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Problems solved by technology

[0003] 1) The recovery time of freeze-dried powder is at least 15-30 minutes, some are longer, and in the process of measurement, due to the serious time lag, online monitoring

Method used

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Embodiment Construction

[0020] A fast luminous bacterial agent supported by signal molecules according to the present invention is characterized in that: the photobacteria freeze-dried powder has a concentration of 10 9 About 10ml of Vibrio fischeri per ml, mixed with centrifuged sludge and 1ml of cryoprotectant, and freeze-dried, with a total weight of 0.5g, and stored at -20°C for 6 months.

[0021] Further, the Vibrio fischeri is a luminous bacterial liquid with a luminous intensity above 700mV obtained through screening, slant culture and shaker liquid culture.

[0022] Further, the composition of the cryoprotectant is prepared by dissolving 15g of skim milk, 5g of glycerin, and 12.5g of trehalose in 100ml of signal molecule solution.

[0023] Further, the signal molecule solution is prepared by the following method: the signal molecule mother solution is mixed with a 6% NaCl (mass fraction) solution to prepare a signal molecule mother solution: a 30:70 mixture of 3% NaCl solution.

[0024] Fur...

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Abstract

The invention discloses quick luminescent bacteria supported based on signal molecule. The quick luminescent bacteria supported based on signal molecule are characterized in that: luminescent bacteria freeze-dried powder is prepared by mixing bacterial mud obtained by centrifuging 10ml of Vibrio fischeri at the concentration of 10<9>/ml and 1ml of cryoprotective agent, and performing freeze drying, and is stored at -20DEG C for 6 months; and the cryoprotective agent is prepared by dissolving 15g of nonfat milk, 5g of glycerol and 12.5g of trehalose in 100ml of signal molecule solution. The invention has the advantages that: by adding signal molecules, the synthesis of luciferase can be promoted, so that the time for the luminescent bacteria freeze-dried powder to restore stable luminescence is shortened from the original 15-30 minutes to 5 minutes; and by adding the composite cryoprotective agent, more bacteria can keep the integrity of cellular structures and the activity of cells during freezing and drying, and can be revived under proper conditions.

Description

technical field [0001] The invention relates to a bacterial agent for detecting water quality toxicity, in particular to a fast luminescent bacterial agent supported by signal molecules. Background technique [0002] Vibrio fischeri is a special marine bacterium that can survive in seawater and emit light. When it encounters toxic substances, its light-emitting ability will be weakened. The change of luminous intensity is related to the toxicity of toxic substances, so the change of luminous intensity when the polluted water body is in contact with luminescent bacteria is often used to judge the acute toxicity of water quality organisms. The use of luminescent bacteria for detection has the characteristics of short time and high sensitivity, and it can also make up for the shortcomings of conventional physical and chemical monitoring, so it is widely used. The national standard method (GT / T15441-1995) detection method for the toxicity test of luminescent bacteria is to use ...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/63
Inventor 叶姜瑜何强翟俊王琳李书钺
Owner CHONGQING UNIV
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