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Method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria

A technology of luminescent bacteria and antibiotics, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms. It can solve the problems of inability to detect delayed toxicity of antibiotic pollutants, inaccurate detection of insoluble or slightly soluble chemical substances, etc., to achieve Delay Toxicity, Improve Assays, Apply Accurate Effects

Inactive Publication Date: 2012-08-15
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide an improved method for detecting the toxicity of antibiotics by luminescent bacteria that is more widely used and accurate, and solves the problem of delayed toxicity of antibiotic pollutants that cannot be detected in the prior art, and the detection of insoluble or slightly soluble chemical substances. Inaccurate detection and other issues

Method used

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  • Method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria
  • Method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria
  • Method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria

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Experimental program
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Embodiment

[0036] 1.1 Preparation of related drugs

[0037] 1.1.1 Configuration of test substance antibiotic mother solution: tetracyclines were dissolved in distilled water for final dissolution, sulfonamides were dissolved by adding dimethyl sulfoxide (not exceeding 1 / 1000 of the total volume) and then water was added for final dissolution.

[0038] 1.1.2 Preparation of concentration related to toxicity test: Dilute the mother liquor to the required concentration with distilled water.

[0039] 1.1.3 Complete medium for luminous bacteria: yeast extract 5g / L, tryptone 5g / L, glycerin 3ml / L, sodium chloride 30g / L, disodium hydrogen phosphate 5g / L, dipotassium hydrogen phosphate 1g / L, pH is 6.5.

[0040] 1.2 Experimental procedure for toxicity determination

[0041]1) Start up and preheat the toxicity detector for 30 minutes, and set it aside;

[0042] 2) Preparing antibiotic mother liquor and diluting the mother liquor to the concentration required for the experiment;

[0043] 3) For t...

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Abstract

The invention provides a method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria. The method comprises the following step of: prolonging the action time of antibiotic-type substances and the luminous bacteria to12-36 hours, preferably 24 hours. In the technical scheme provided by the invention, the action time of the antibiotic-type substances and the luminous bacteria is prolonged, so that the proliferation of the luminous bacteria during the period is ensured; and through the method, good timeliness of the accurate characterization of the biotoxicity of antibiotic can be realized, the detection result is more accurate on the basis of guaranteeing the timeliness, and the detection of toxicity delay of antibiotic-type pollutants is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the technical field of luminescent bacteria detection, in particular to a method for detecting the toxicity of antibiotic substances by using luminescent bacteria. Background technique [0002] Luminescent bacteria are a kind of ubiquitous bacteria that can emit blue-green visible light, which are mainly distributed in the ocean, or live freely, or parasitize marine or terrestrial animals. [0003] The luminescent process of luminescent bacteria is a photorespiratory process, which is a side branch on the electron transport chain, and the reduced flavin mononucleotide (FMNH 2 ) acts as the substrate of the reaction, the luciferase plays a catalytic role, and the long-chain aliphatic aldehyde acts as a "modification" to change the structure of the luciferase. Luciferase catalyzed by molecular oxygen (FMNH 2 ) and aliphatic aldehyde (RCHO), this oxidation-reduction reaction results i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
Inventor 李娟英王静彭自然
Owner SHANGHAI OCEAN UNIV
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