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Method for evaluating water quality health risk with chromium ion as standard toxic substance

A chromium ion and water quality technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, water testing, etc., can solve the problem of insensitivity of chlorinated organics, easy transmission, inability to accurately and comprehensively know the actual risk status of water quality and other problems, to achieve the effect of accurate water toxicity and easy results

Active Publication Date: 2011-09-07
济南市供排水监测中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual application, the SOS / umu test system is not sensitive to chlorinated organic substances, which is not conducive to the detection of disinfection by-products in water bodies, especially in drinking water. Salmonella typhimurium is a pathogenic bacterium and is easy to spread.
Most micronucleus tests and single-cell gel electrophoresis tests are not intuitive enough, and the classification of toxicity grades is somewhat subjective
It can be seen that if only one technology is used to evaluate the toxicity of water quality, the evaluation results for water quality with complex pollutants may be inaccurate, and there will also be a certain one-sidedness, and the actual risk of water quality cannot be accurately and comprehensively known situation

Method used

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  • Method for evaluating water quality health risk with chromium ion as standard toxic substance
  • Method for evaluating water quality health risk with chromium ion as standard toxic substance
  • Method for evaluating water quality health risk with chromium ion as standard toxic substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Take the surface water of this city with 0.04mg / L deltamethrin as an example to evaluate the degree of pollution. The water quality conditions of the surface water are: pH 8.07, temperature 25°C, dissolved oxygen 9mg / L, hardness 305 mg / L.

[0094] Methods as below:

[0095] 1. Biological fish toxicity test

[0096] (1) Select medaka fish (Medakafish, Oryzias latipes) with a body length between 2 and 3 cm, and put them in standard water (the standard water is prepared with distilled water or deionized water, and its water quality conditions are consistent with surface water) for 7 days. , the mortality rate during domestication is less than 10%, if it is higher than 10%, change a batch of fish and re-domesticate. Fasting began 1 day before the test; standard water was prepared with distilled water or deionized water, and its water quality conditions were consistent with surface water;

[0097] (2) Collect the water samples to be tested. After fully aerating, add 15 m...

Embodiment 2

[0104] Taking the influent water of a daily urban water plant as an example, the pollution degree is evaluated as follows:

[0105] 1. Biological fish toxicity test

[0106] Carry out biological fish toxicity test according to the method of embodiment 1, find no dead fish phenomenon within 96 hours through observation, then continue to carry out luminescent bacteria toxicity test;

[0107] 2. Toxicity test of luminescent bacteria: Toxicity test of luminescent bacteria with Vibrio Qinghai

[0108] (1) Prepare bacterial suspension:

[0109] a. Cultivate Vibrio Qinghai on LB solid medium at 22°C for 24h, inoculate 30 single colony strains into 150ml liquid LB medium, and culture with shaking at 22°C for 20h;

[0110] b. Centrifuge the liquid medium containing luminescent bacteria under the centrifugation condition of 4000r / min, and then suspend it with sterile physiological saline to obtain a bacterial suspension with an OD value of 0.9;

[0111] (2) Collect the water sample t...

Embodiment 3

[0115] Taking the influent water of a water plant as an example, the pollution degree is evaluated as follows:

[0116] 1. Biological fish toxicity test

[0117] Carry out biological fish toxicity test according to the method of embodiment 1, find no dead fish phenomenon within 96 hours through observation, then continue to carry out luminescent bacteria toxicity test;

[0118] 2. Toxicity test of luminescent bacteria

[0119] According to the method of Example 2, the luminescent bacteria toxicity test was carried out, and the luminescent inhibition rate of Vibrio Qinghai was detected to be lower than 40%, and the SOS / umu test was continued;

[0120] 3. SOS / umu test

[0121] (1) Concentration and enrichment of water samples: Take 4L of the water sample to be tested, absorb it with the activated XAD-2 resin at a rate of 40mL / min, then elute with ethyl acetate, and blow off the eluate with nitrogen at 50°C Ethyl acetate, redissolve the sample with a certain volume of DMSO to ...

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Abstract

The invention relates to a method for evaluating water quality health risk with a chromium ion as a standard toxic substance, comprising the following steps: a toxicity test is conducted on biological fish, if dead fish occurs in 96h, Cr<6+> concentration is used for representing water quality toxicity, and if dead fish does not occur, a luminescent bacteria toxicity test is conducted; if the luminescent inhibition rate is higher than 40%, the Cr<6+> concentration is used for representing the water quality toxicity, and if the luminescent inhibition rate is lower than 40%, an SOS / umu test is conducted; if a test result is positive reaction, the Cr<6+> concentration is used for representing the water quality toxicity, and if a test result is negative reaction, a broad bean root tip micronucleus test is conducted; and if a micronucleus permillage ratio is more than 1.5, the Cr<6+> concentration is used for representing the water quality toxicity, and if the micronucleus permillage ratio is less than 1.5, a unicellular gel electrophoresis test is conducted and the Cr<6+> concentration is used for representing the water quality toxicity. In the invention, the steps are closely related to each other so that both the low pollutant content and the high pollutant content in water quality can be detected, the detection time is shorter, the result is accurate and easy to unify, and quantification evaluation of the water quality toxicity can be realized.

Description

technical field [0001] The present invention relates to a kind of method for evaluating the health risk of drinking water, particularly relate to a kind of 6+ A method for comprehensive evaluation of drinking water health risks for standard toxic substances. Background technique [0002] In order to ensure drinking water safety and reduce the threat of environmental degradation to drinking water quality, people often use various methods to evaluate water quality to effectively grasp the health risks caused by drinking water. [0003] Water is an indispensable resource for us. With the deterioration of the environment, many water sources have been polluted. In order to ensure the safety of drinking water and reduce the threat of environmental deterioration to the quality of drinking water, the evaluation of water quality health risks has become more and more important. more important. Water quality detection based on physical and chemical analysis methods can directly analy...

Claims

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Application Information

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IPC IPC(8): G01N33/18G01N21/00C12Q1/18C12Q1/10C12Q1/04G01N27/447
Inventor 贾瑞宝孙韶华宋艳李力
Owner 济南市供排水监测中心
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