Method for rapidly detecting veterinary drug residues in animal products by using luminescent bacteria

Abstract

The invention discloses a method for rapidly detecting veterinary drug residues in animal products by using luminescent bacteria. Vibrio qinghaiensis Q67 and brilliant luminescent bacterium T3 are used as toxicity test strains, by measuring relative luminescence intensity at different mass concentrations and the half inhibitory effect concentration (EC50) of ten veterinary drugs of the vibrio qinghaiensis Q67 and the brilliant luminescent bacterium T3 in the ten common veterinary drugs such as sulfonamides, furans and quinolones, the law of function of the ten veterinary drugs on the luminescent bacteria and acute toxic effects in animal products are studied; secondary universal composite test design and statistical method is adopted to study the combined toxicity of various quinolone veterinary drugs against the vibrio qinghaiensis and reveal the total toxic effects and interactions of the various quinolone veterinary drugs during coexisting; the law of action and acute toxic effectsof the veterinary drug residues in pork extracts against the vibrio qinghaiensis are preliminarily explored; and therefore, a theoretical basis for establishing a method for detecting the veterinary drug residues by the luminescent bacteria and a safety evaluation system for the animal products is provided. According to the method for rapidly detecting the veterinary drug residues in the animal products by using the luminescent bacteria, materials used in the method for rapidly detecting the veterinary drug residues in the animal products by using the luminescent bacteria is cheap and easy toobtain, and the route is simple, and the requirements of the ministry of agriculture for detecting the sulfonamides veterinary drugs in the animal products are met.

Description

technical field [0001] The invention belongs to the technical field of biology and new medicine, and specifically relates to the field of veterinary drug detection. Background technique [0002] In recent years, with the development of agriculture and animal husbandry, the abuse of veterinary drugs, pesticides and additives has caused serious residues of harmful substances in animal products, which not only brought potential harm to consumers' health, but also affected the development of animal husbandry. The detection technology of veterinary drug residues has also more and more attention. At present, liquid phase, LC-MS and other technologies are mostly used at home and abroad to detect veterinary drug residues. However, these technologies have disadvantages such as complicated operation, long time and high detection cost, and are not suitable for online detection. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to provide a method for rapid de...

AI Technical Summary

Problems solved by technology

At present, liquid phase and liquid chromatography-mass spectrometry are mostly used at home and abroad to detect veterinary drug residues, but these technologies have disadvantages such as complex operation, long time, high detection cost, etc., and are not suitable for online detection