Detection method for acute biological toxicities of pollutants on dust-haze day

A technology for biotoxicity and detection methods, applied in the direction of chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problems of acute biological toxicity of compound pollutants that have not been tested, and achieve fast detection speed and high sensitivity. Effect

Inactive Publication Date: 2016-10-05
SHANGHAI NAN YANG MODEL HIGH SCHOOL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no method for testing the acute biological toxicity of complex pollutants in the air of haze days

Method used

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  • Detection method for acute biological toxicities of pollutants on dust-haze day
  • Detection method for acute biological toxicities of pollutants on dust-haze day
  • Detection method for acute biological toxicities of pollutants on dust-haze day

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Air biological acute toxicity detection in a certain winter in the suburbs of Shanghai

[0036] 1. Sampling

[0037] (1) Take air samples from a winter haze day in Shanghai, and suck them for different times, 3060 and 120 minutes, respectively. Take three samples in parallel for each time period, and dissolve them in sterile water.

[0038] (2) Control water sample solution: use distilled water, add NaCl to a final concentration of 0.8%.

[0039] 2. Luminescence detection

[0040] The BHP95114 water quality toxicity analyzer produced by Beijing Hamamatsu Photon Technology Co., Ltd. was used as the test instrument.

[0041] Use the resuscitation solution of freeze-dried powder bacterial liquid.

[0042] Prepare three small test cups, add 2ml water sample to each small cup, and set it as three parallel samples; take three test small cups for the control sample solution, add 2ml each, set three parallel samples, and add frozen 0.05ml of dry powder resuscitat...

Embodiment 2

[0045] Example 2: Detection of biological acute toxicity of atmospheric samples in winter and spring in Shanghai urban area

[0046] 1. Sampling

[0047] (1) Take the air samples from March 25th and April 10th in a certain winter and spring season in an urban area, dissolve them in sterile water, add NaCl to a final concentration of 0.8%, and use them as samples to be tested.

[0048] (2) A blank water sample control was prepared by adding NaCl to distilled water to a final concentration of 0.8%.

[0049] 2. Luminescence detection

[0050] The BHP95114 water quality toxicity analyzer produced by Beijing Hamamatsu Photon Technology Co., Ltd. was used as the test instrument.

[0051] Use the resuscitation solution of freeze-dried powder bacterial liquid.

[0052] Prepare three test small cups, add 2ml of distilled water samples to each small cup, set up three parallel samples, and take three test small cups for blank water sample control, add 2ml each, and set up three parall...

Embodiment 3

[0056] Example 3: Bioacute Toxicity Detection of Atmospheric Samples at an Intersection in Shanghai Urban Area

[0057] 1. Sampling

[0058] (1) Take samples from busy traffic intersections in Shanghai urban area, collect air samples of wearing different types of masks for 20 minutes at the intersection, and 2 minutes of car exhaust, dissolve them in sterile water, add NaCl to a final concentration of 0.8%, and use it as the test sample .

[0059] (2) Blank water sample control, prepared by adding NaCl to distilled water to a final concentration of 0.8%.

[0060] 2. Luminescence detection

[0061] The BHP95114 water quality toxicity analyzer produced by Beijing Hamamatsu Photon Technology Co., Ltd. was used as the test instrument.

[0062] Use the resuscitation solution of freeze-dried powder bacterial liquid.

[0063] Prepare three test small cups, add 2ml of distilled water samples to each small cup, set up three parallel samples, and take three test small cups for blank...

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Abstract

The invention discloses a detection method for acute biological toxicities of pollutants on a dust-haze day. The detection method specifically comprises the following steps that air samples on the dust-haze day are collected and dissolved in sterile water, the light intensity of the air pollutants dissolved in water is measured through freshwater luminescent bacteria-Qinghai vibrio Q67, and the comprehensive acute toxicity effect of the air pollutants on the dust-haze day on bionts is detected through comparison with a blank group. Compared with other biological acute toxicity testing methods, the detection method has the advantages that the luminous bacterial method used for measuring the acute biological toxicities of the dust-haze day is high in sensitivity and high in detection speed, a result can be obtained within 0.5 h, and the levels of the acute biological toxicities of the air pollutants can be judged. The detection method has actual application value for detection and prevention of comprehensive toxicities of the air pollutants on the dust-haze day.

Description

technical field [0001] The invention relates to a haze day acute biological toxicity detection technology, in particular to a haze day acute biological toxicity detection sample preparation and detection method, belonging to the technical field of atmospheric pollutant toxicity detection. Background technique [0002] In winter and spring, due to the temperature inversion on the ground, it is easy to form a foggy day. If there are particulate matter in the air, the sky will become gray, and this kind of weather is called a haze day. Haze days are caused by particulate matter pollution, which will have a serious impact on the human body, especially the health of the respiratory system. Fine particles such as solid particles or liquid droplets with a diameter less than or equal to 2.5 microns in the air are more likely to cause damage to the respiratory system and cardiovascular system, including respiratory tract irritation, coughing, dyspnea, reduced lung function, aggravate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 谢奕琪
Owner SHANGHAI NAN YANG MODEL HIGH SCHOOL
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