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Method for detecting acute toxicity of rare earth tailing pond surrounding groundwater pollution by using freshwater luminescent bacteria

A technology for groundwater pollution and light-emitting bacteria is applied in the field of environmental pollution detection and evaluation, and achieves the effects of simple and time-saving preparation, easy popularization and application, and fast and convenient testing.

Inactive Publication Date: 2016-05-18
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for acute toxicity testing of groundwater pollution around rare earth tailings ponds using freshwater luminescent bacteria Vibrio qinghai, to overcome the defects and deficiencies of previous biological monitoring methods, and to reduce the risks caused by various factors in traditional methods. may affect the detection results more reliable

Method used

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  • Method for detecting acute toxicity of rare earth tailing pond surrounding groundwater pollution by using freshwater luminescent bacteria
  • Method for detecting acute toxicity of rare earth tailing pond surrounding groundwater pollution by using freshwater luminescent bacteria
  • Method for detecting acute toxicity of rare earth tailing pond surrounding groundwater pollution by using freshwater luminescent bacteria

Examples

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Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Selection test of reaction time and pH value

[0050] The test optimizes the test reaction time and the applicable range of sample pH in the test method.

[0051] Separately prepare and dilute different concentrations of Na 2 SO 4 solution (500, 1000 and 10000 mg / L), on the basis of this solution, the optimization experiment of reaction time and pH value was carried out.

[0052] Reaction time optimization: Set different reaction times of 0, 3, 7, 10, 15, 20, 30, 40, 60, 80, 100 and 120min respectively.

[0053] pH Optimization: Adjust the pH of the sample to be tested to 4, 5, 6, 7, 8, 9, 10, 11, and 12, respectively.

[0054] The reaction time optimization results can be found in figure 1 , figure 1 is the effect of different reaction times on the test luminescence intensity, where CK is 0.85wt% NaCl, 500mg / L, 1000mg / L and 10000mg / L respectively represent 500mg / L Na 2 SO 4 , 1000mg / L Na 2 SO 4 and 10000mg / L Na 2 SO 4 . from figure 1 It can be s...

Embodiment 2

[0057] Example 2: Selection test of sample diluent

[0058] During the determination process, since the sample to be tested may contain a relatively high concentration of some toxic substances, the luminescent bacteria do not respond to the toxicity, and accurate detection results cannot be obtained. Therefore, it is necessary to properly dilute the sample to be tested before performing the determination.

[0059] Prepare three dilutions of simulated natural water (see Table 2 for ingredients), 0.85wt% NaCl and 10% lactose solution respectively, and prepare or dilute them into 1000mg / L Na with these three dilution solutions. 2 SO 4 solution. The relative inhibition rates of the samples in these three different dilutions were determined according to the method described above. Figure 4 . Figure 4 is the effect of different dilutions on the relative inhibition rate, in which CK is without Na 2 SO 4 The corresponding dilutions of , from Figure 4 It can be seen that the ...

Embodiment 3

[0062] Example 3: Influence test of sample turbidity

[0063] The turbidity of the sample will affect the reading of the luminescence intensity of the luminescent bacteria to a certain extent. If the sample turbidity is too large, the sample needs to be filtered or precipitated. In this example, fine sand is used, and after the sample is fully ground, it is passed through a 100-mesh sieve. Stir with distilled water for 24h and wash several times. Dilute into 14 dilution samples (ONTU-4800NTU) of different turbidity with 0.85wt% NaCl, measure the turbidity of each dilution with a turbidimeter and measure the light inhibition rate of luminescent bacteria according to the above method, the results are shown in Figure 5 , Figure 5 It is a graph showing the effect of different turbidity of the samples on the relative inhibition rate of Qinghai solitary bacteria.

[0064] from Figure 5 It can be seen that with the decrease of the turbidity of the dilution solution, the relati...

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Abstract

The invention discloses a method for detecting acute toxicity of rare earth tailing pond surrounding groundwater pollution by using freshwater luminescent bacteria. The freshwater luminescent bacteria are Vibrio qinghaiensis Q67; the method comprises steps as follows: a to-be-detected sample is treated and diluted, and the pH value is adjusted; an activated Vibrio qinghaiensis Q67 solution is added to the to-be-detected sample for a reaction; meanwhile, a diluent for dilution replaces the to-be-detected sample to serve as a contrast; the luminescent intensity of the to-be-detected sample and the luminescent intensity of the contrast are detected by a bioluminescence tester; the result is calculated, standard reference poison ZnSO4 is set, and the toxicity effect is judged according to the result. The method is rapid, sensitive, accurate and convenient to popularize and apply. Meanwhile, Vibrio qinghaiensis, the freshwater luminescent bacteria, is used as a test strain, and the influence caused by salinity required by marine luminescent bacteria on luminescence is avoided. The reference poison is ZnSO4, and the influence on the ecological environment and human health is reduced.

Description

technical field [0001] The invention relates to the technical field of environmental pollution detection and evaluation, in particular to a method for detecting the acute toxicity of groundwater pollution around rare earth tailings ponds by using freshwater luminescent bacteria. Background technique [0002] At present, in the field of environmental monitoring, the types and concentrations of pollutants can generally be determined quickly and sensitively by various instruments and chemical analysis methods, but most of the measurement items or parameters need to be regularly sampled. Therefore, it can only reflect the instantaneous pollutant concentration of sampling, and cannot reflect the changes that have occurred in the environment. While biological monitoring was carried out in some countries in the early 20th century, biological monitoring of water pollution has become an active research field in recent years. Biomonitoring is the basis for the detection and evaluatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/18
CPCG01N21/763G01N33/1866
Inventor 蔡超安新丽张又弛朱永官
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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