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This invention discloses a multiplex PCR primer combination, kit, and detection method for detecting Echinococcus granulosus. The multiplex PCR primer combination includes a second primer combination and a third primer combination: the second primer combination consists of upstream and downstream primers specific to Echinococcus multilocularis, with sequences shown in SEQ ID NO. 3 and SEQ ID NO. 4, respectively; the third primer combination consists of upstream and downstream primers specific to Echinococcus shiquensis, with sequences shown in SEQ ID NO. 5 and SEQ ID NO. 6, respectively. This invention saves more time than conventional PCR, is more cost-effective than quantitative real-time PCR, and has lower requirements for operators and equipment, showing great promise for the detection and control of echinococcosis.