The invention provides a
vaginal secretion staining solution, a preparation method and a method for
staining by applying the
vaginal secretion staining solution, and belongs to the technical field of
cell staining. The alkaline
toluidine blue basic dye is used as a coloring agent, the
phosphate buffer solution is used as a
solvent, the
sodium hydroxide is used as a
regulator, the components are simple, the preparation is convenient, the
dyeing method is rapid, epithelial cells, leukocytes and
bacilli can be dyed, trichomonad and mould cannot be dyed, the leukocytes and the trichomonad can be obviously distinguished through
color difference, and the method is suitable for large-scale popularization and application. In addition, the
colored bacilli can intuitively assist in cleanliness discrimination and clue
cell identification, so that personnel misjudgment caused by similar forms of detection targets can be avoided, and the problems that the current dye liquor has many components, the preparation method is tedious, the
dyeing method is complex, the detection time is short and the like are solved. And the problem of lack of a sample tube dropwise adding dye liquor proportioning scheme is solved.