Method for coupling carboxyl microspheres with amino groups, sensitizing latex and kit
A microsphere and coupling technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited reaction conditions, cumbersome operation steps, harsh operating conditions, etc., to achieve simple operation, overcome cumbersome operation, and high-efficiency coupling Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] According to the antistreptolysin "O" test kit prepared by the preferred scheme of the present invention, its steps are:
[0043](1) Preparation: prepare polystyrene latex microspheres of 155nm, and dilute the latex microspheres with 50mM / L 2-(N-morpholine)ethanesulfonic acid at pH 6.0 to a final concentration of 1.5% w / v to obtain latex microspheres. Ball solution; N,N'-carbonyldiimidazole (CDI) was dissolved with 50mM / L 2-(N-morpholine)ethanesulfonic acid at pH 6.0 to obtain a CDI solution with a concentration of 0.01g / mL;
[0044] (2) Activation: Based on the weight of the latex microspheres, add each mg of microspheres dropwise to the latex microsphere solution in the proportion of 50 μL of CDI solution, shake and mix, shake and activate at 25°C for 25 minutes, and the shaking speed is 200 rpm / min. Obtain activated latex microspheres;
[0045] (3) Add 50mM / L phosphate buffer to adjust the pH to 8.0;
[0046] (4) Coupling: based on the weight of the latex microsphe...
Embodiment 2
[0050] According to the C-reactive protein kit prepared by the preferred scheme of the present invention, its steps are:
[0051] (1) Preparation: Prepare 107nm polystyrene latex microspheres, and the latex microspheres are diluted with 50mM / L 2-(N-morpholine)ethanesulfonic acid at pH 6.0 to a final concentration of 3% w / v to obtain latex microspheres. Ball solution; N,N'-carbonyldiimidazole (CDI) was dissolved with 50mM / L 2-(N-morpholine)ethanesulfonic acid at pH 6.0 to obtain a CDI solution with a concentration of 0.01g / mL;
[0052] (2) Activation: Based on the weight of the latex microspheres, add each mg of microspheres dropwise to the latex microsphere solution in the proportion of 80 μL of CDI solution, shake and mix, shake and activate at 30 ° C for 30 minutes, and the shaking speed is 100 rpm / min. Obtain activated latex microspheres;
[0053] (3) Add 50mM / L borate buffer to adjust the pH to 7.5;
[0054] (4) Coupling: based on the weight of the latex microspheres, ad...
Embodiment 3
[0059] According to the β2 microglobulin test kit prepared by the preferred scheme of the present invention, its steps are:
[0060] (1) Preparation: Prepare 123nm polystyrene latex microspheres, which are diluted with 50mM / L 2-(N-morpholine)ethanesulfonic acid at pH 6.0 to a final concentration of 1.8% w / v to obtain latex microspheres. Ball solution; N,N'-carbonyldiimidazole (CDI) was dissolved with 50mM / L 2-(N-morpholine)ethanesulfonic acid at pH 6.0 to obtain a CDI solution with a concentration of 0.01g / mL;
[0061] (2) Activation: Based on the weight of the latex microspheres, add each mg of microspheres dropwise to the latex microsphere solution in the proportion of 60 μL of CDI solution, shake and mix, shake and activate at 37°C for 20 minutes, and the shaking speed is 200 rpm / min. Obtain activated latex microspheres;
[0062] (3) Add 50mM / L borate buffer to adjust the pH to 7.5;
[0063] (4) Coupling: based on the weight of the latex microspheres, add the polyclonal a...
PUM
Property | Measurement | Unit |
---|---|---|
Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com