15 results about "Nephelometry" patented technology

The invention relates to a sample analyzer, a protein analysis method and a protein analysis reagent. Preparing a first test solution by adopting a to-be-detected sample and the colorimetric method reagent, and detecting the first test solution by adopting a colorimetric method to obtain a colorimetric method detection result of the protein in the to-be-detected sample. Whether turbidimetric detection is carried out or not is judged on the basis of the colorimetric detection result, if not, the concentration of protein in the to-be-detected sample is determined on the basis of the colorimetric detection result, and if yes, a second test solution is prepared from the to-be-detected sample and a turbidimetric reagent, and the second test solution is detected through turbidimetry; and determining the concentration of the protein in the to-be-detected sample on the basis of the turbidimetric detection result and an optional colorimetric detection result. According to the present invention, the protein in the sample can be detected by integrating the colorimetric method and the turbidimetric method, and the linear detection range of the one-time protein detection is improved.

The invention discloses a latex enhanced immunoturbidimetry detection reagent for glycosylated hemoglobin and a use method of the latex enhanced immunoturbidimetry detection reagent, and relates to the technical field of medical examination. The latex enhanced immunoturbidimetry detection reagent for glycosylated hemoglobin comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises first nano microspheres; and the reagent R2 comprises a second nano microsphere coated with a monoclonal antibody of HbA1c. According to the reagent R2, the nano microspheres are coated with the monoclonal antibody of HbA1c, the stability risk caused by the fact that balance needs to be established for immunoreaction of multiple antibodies and antigens is avoided through the monoclonal antibody, the problem that a glycosylated hemoglobin immunoturbidimetry detection reagent is insufficient in stability can be solved, the nano microspheres are arranged on the reagent R2, and therefore the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved, and the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved. By utilizing the characteristic that the volume of an antigen-antibody conjugate can be increased after the nano-microspheres are combined with the HbA1c monoclonal antibody, the turbidimetric degree of latex is effectively amplified, and the detection sensitivity can be improved.

The invention discloses a method for detecting the activity of lysozyme, which evaluates the activity of the lysozyme by detecting the release amount of nuclease in bacterial content, and realizes high-sensitivity quantification of the lysozyme by designing a special nuclease fluorescent probe substrate. Compared with the traditional nephelometry, the method has the advantages of remarkable sensitivity and robustness. According to the method, natural bacteria are used as a lysozyme substrate, so that the false negative problem that a fluorescence modified bacterial cell wall substrate is difficult to recognize and hydrolyze by part of lysozyme in the existing commodity kit is solved. Compared with an existing fluorescence method commercialized kit, the method has the advantages of being wide in application range and accurate in result.

A product for diagnosing lung cancer, wherein the product comprises a reagent used for detecting a biomarker in a subject sample, and the biomarker includes PCDH18, CDKN2A and / or HOXA9. The reagent used for determining expression level of the biomarker on the subject sample by quantitative PCR, NGS, Northern blot, Southern blot, microarray, SAGE, immunoassay or mass spectrometry. Methods of immunoassay include ELISA, EIA, agglutination test, nephelometry, turbidimetry, Western blot, immunoprecipitation, immunocytochemistry, flow cytometry and Luminex assay. The reagent comprises a primer, probe and an antibody and the product comprises a kit, nucleic acid membrane strip, a preparation and a chip. The lung cancer may be lung squamous cell carcinoma.

The application provides a latex immunoturbidimetric detection reagent, a kit and a detection method. The latex immunoturbidimetric detection reagent comprises a buffer and an ionic liquid, the ionic liquid comprises an ionic liquid containing an imidazole structure, the ionic liquid containing the imidazole structure comprises 1-alkyl-3-methyl imidazole halide or 1-alkyl-3-methyl imidazole fluoride, the halogen in the 1-alkyl-3-methyl imidazole halide comprises chlorine, bromine or iodine, and the alkyl in the 1-alkyl-3-methyl imidazole halide or 1-alkyl-3-methyl imidazole fluoride comprises alkyl containing 2, 3, 4, 5, 6 or 7 C atoms. The application can solve the problem of a small linear range in the prior art latex turbidimetric method, and is suitable for the field of immunoturbidimetric detection.

A turbidity instrument to measure turbidity in a liquid sample by nephelometry which includes a primary optical chamber with source and detector channels extending therefrom and a removable secondary optical chamber having windows configured to align with the source and detector channels when in position, where the secondary optical chamber fluidly isolates the primary optical chamber from an exterior of the instrument when in position.

The embodiment of the present application discloses a method for measuring an analyte based on a turbidimetry method, which comprises the following steps: acquiring at least one prediction time period within a maximum measurement duration and a calibration curve corresponding to the at least one prediction time period; obtaining an estimated concentration value of the analyte according to a reaction degree of the analyte in the at least one prediction time period and the calibration curve corresponding to the at least one prediction time period; determining a final measurement time of the analyte according to the estimated concentration value and a preset corresponding relationship between an analyte concentration value and a measurement time; and determining an actual concentration value of the analyte when the final measurement time is reached. The present application also relates to a measuring device for measuring an analyte based on a turbidimetry method, a sample analyzer for measuring an analyte based on a turbidimetry method and a computer readable storage medium.

The utility model discloses a non-specific immunoglobulin detection kit for cattle and sheep, which relates to the technical field of livestock raising and comprises a kit body, a centrifugal tube bracket, a centrifugal tube I, a Pasteur pipette and a centrifugal tube II, the centrifugal tube bracket is used for supporting the centrifugal tube I and the centrifugal tube II, and the Pasteur pipette I is used for supporting the Pasteur pipette II. The first centrifugal tube is used for containing a sodium sulfate sterile solution, the pasteur pipettes are used for sucking serum samples to be detected, the number of the pasteur pipettes is multiple, and the second centrifugal tubes are used for containing successful passive transformation serum control samples and passive transformation failure serum control samples respectively; the non-specific immunoglobulin detection kit for cattle and sheep is used for detecting non-specific immunoglobulin in serum of newborn calves and lambs, semi-quantification is carried out through turbidimetry, and the passive conversion effect of a maternal antibody of a young livestock can be evaluated.

The invention discloses determination of non-biological turbidity of cereal brewed cooking wine based on absorbance difference, belongs to the technical field of detection, and the cereal brewed cooking wine as important flavoring wine is widely applied in the fields of cooking and catering. The problem of non-biological turbidity is long-standing, and the consumption experience is influenced. The traditional nephelometry and light transmittance method can be used for determining the turbidity of a wine sample, but have certain limitation, and the research establishes a novel simple, convenient and scientific method which takes absorbance difference as a basis and takes EBC turbidity as a reference to evaluate the turbidity of the grain brewed cooking wine, so that the method is expected to be better suitable for determining the turbidity of the grain brewed cooking wine.

Provided are a particle for immunoturbidimetry, a reagent, a test kit, and a method for detecting a target substance, each of which can detect a trace component in a low concentration region and improve storage stability. The particle for immunoturbidimetry is a particle for immunoturbidimetry including a first resin and titanium oxide, wherein the particle for immunoturbidimetry includes a first layer containing the first resin and a second layer containing the titanium oxide, wherein the second layer is arranged on the outside of the first layer, and wherein the density of the titanium oxide is 3.40 g / cm 3 Hereinafter, and wherein the content of the titanium oxide in the particle for immunoturbidimetry is 10 mass% or more and 80 mass% or less.

The invention discloses an immunoglobulin D transmittance-scattering latex turbidimetry detection kit, and belongs to the technical field of biological detection. The immunoglobulin D transmission-scattering latex turbidimetry detection kit disclosed by the invention comprises a first reagent R1, a second reagent R2, a calibration product and a quality control product. The kit disclosed by the invention has the advantages of universality (relatively low cost) and detection result reliability (good repeatability of a low value and no false negative leak detection of a high value).

The application provides a double-wavelength spectrophotometric turbidimetry colorimetric probe and application thereof, the colorimetric probe comprises a light source, a sample cell and a photoelectric receiving assembly arranged in sequence; the photoelectric receiving assembly comprises a first photoelectric receiving assembly and a second photoelectric receiving assembly; light in the light source is divided into two paths after passing through the sample cell, and light of different wavelengths enters the first photoelectric receiving assembly and the second photoelectric receiving assembly respectively, and a first electric signal is output by the first photoelectric receiving assembly, and a second electric signal is output by the second photoelectric receiving assembly. Compared with the prior art, the colorimetric probe provided by the application, in combination with the use of the double-wavelength spectrophotometric turbidimetry, can accurately calculate the concentration of chloride ions in the acid copper tank solution through the absorbance of different wavelengths, not only the detection speed is fast, but also the accuracy and precision of measurement can be ensured when the content of chloride ions is 0.1 mg / L, and the problem of low data accuracy of the detection method for the detection of trace chloride ions in the acid copper tank solution is effectively solved.

Systems and methods are provided for using an additively manufactured vehicle, such as an UUV, with additively manufactured modules. The vehicle may be configurable such that additively manufactured modules or components may be detachably connected to the vehicle by hand, without the use of tools. Such modules may include connectors adapted to securely attach additional modules that may be detached by hand, without the use of tools. The additively manufactured modules may include ball bearings for rotating modules such as propellers and thrusters, and clips or tabs for detachable connection. The modules may include optical components for communications between a swarm of unmanned vehicles. Such optical modules for underwater vehicles may utilize nephelometry and / or turbidimetry to improve communications parameters based on scattered light measurements.

The invention relates to application of a low-ionic-strength buffer substance and a detection reagent, in particular to application of the low-ionic-strength buffer substance in preparation of a latex microsphere immunoturbidimetry detection reagent. The requirements of an immunoturbidimetry detection reagent for high buffer capacity and stable pH can be met, the defects that strong electrolyte ions in an existing buffer system influence the dispersion stability of hydrophobic latex microspheres and interfere adsorption of substances to be detected on the surfaces of the capture type latex microspheres and the like can be overcome, a stable liquid environment is provided for immunoturbidimetry reaction, and the detection sensitivity is high. The method has the capacity of resisting carrying and cross contamination, interference is reduced, and the accuracy of a detection result is ensured.

The application discloses an antibacterial peptide segment intercepted from a Stomoxys calcitrans IDGF4 protein and application of the antibacterial peptide segment in preparation of antibacterial drugs. The application screens short peptide fragments in different positions of the Stomoxys calcitrans IDGF4 protein, obtains three candidate peptides through chemical synthesis, and systematically evaluates antibacterial performances of the three candidate peptides. The antibacterial experiment results show that the short peptide with the amino acid sequence shown in SEQ ID No. 2 can effectively inhibit growth of salmonella and staphylococcus aureus at a concentration of 1 mg / mL, the antibacterial effect is confirmed in an antibacterial circle experiment, an enzyme label turbidimetry method and growth curve monitoring, and the short peptide shows stable and significant antibacterial activity, indicating that the peptide segment can be used as a new antibacterial molecule. The application provides a new candidate sequence for development of efficient and low drug resistance risk antibacterial peptides, and has application potential in preparation of clinical antibacterial drugs.