The invention discloses a latex enhanced
immunoturbidimetry detection
reagent for glycosylated
hemoglobin and a use method of the latex enhanced
immunoturbidimetry detection
reagent, and relates to the technical field of medical examination. The latex enhanced
immunoturbidimetry detection
reagent for glycosylated
hemoglobin comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises first nano microspheres; and the reagent R2 comprises a second nano
microsphere coated with a
monoclonal antibody of HbA1c. According to the reagent R2, the nano microspheres are coated with the
monoclonal antibody of HbA1c, the stability risk caused by the fact that balance needs to be established for immunoreaction of multiple antibodies and antigens is avoided through the
monoclonal antibody, the problem that a glycosylated
hemoglobin immunoturbidimetry detection reagent is insufficient in stability can be solved, the nano microspheres are arranged on the reagent R2, and therefore the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved, and the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved. By utilizing the characteristic that the volume of an
antigen-
antibody conjugate can be increased after the nano-microspheres are combined with the HbA1c
monoclonal antibody, the turbidimetric degree of latex is effectively amplified, and the detection sensitivity can be improved.