Apolipoprotein E test kit

Abstract

The invention discloses an apolipoprotein E test kit. The kit comprises a box body, wherein from left to right, the top of the box body is provided with a groove, a dropper placement groove and a placement groove in sequence, the left of the bottom of the inner cavity of the groove is actively connected with a second cover plate through a hinge, a standard solution placement groove is arranged at the lower end of the groove in the inner cavity of the box body, a standard solution is injected in the inner cavity of the standard solution placement groove, the bottom of the inner cavity of the dropper placement groove is fixedly connected with a second placement plate, a dropper is inserted in the inner cavity of the second placement plate, and the bottom of the inner cavity of the placement groove is fixedly connected with a first placement plate. The method adopts nephelometry and comprises steps of fusing ApoE antigen in a sample with a R2 reagent 9 in a solution, so as to form an antigen-antibody compound immediately and cause certain turbidity, wherein the degree of the turbidity is directly proportional to content of ApoE antigen when a certain amount of antibody exists, comparing the solution with a calibration solution treated the same way and calculating content of ApoE in unknown samples.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to an apolipoprotein E detection kit. Background technique [0002] Apolipoprotein E (Apolipoprotein E, ApoE) is a polymorphic protein that participates in the transformation and metabolism of lipoproteins. Its gene can regulate many biological functions and is related to the pathogenesis of many ophthalmic diseases. ApoE and its gene polymorphism Apolipoprotein E is one of the current medical research hotspots. To explore the internal relationship between the two has important clinical application value for the prevention, diagnosis and treatment of eye diseases. Apolipoprotein E mainly exists in CM, VLDL, IDL and some In HDL, the concentration of ApoE in normal human plasma is 0.03-0.05g / L, but the existing apolipoprotein E detection kits cannot quantitatively measure the content of apolipoprotein E (APOE) in human serum in vitro. bring inconvenience. Contents of the ...

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Problems solved by technology

[0002] Apolipoprotein E (Apolipoprotein E, ApoE) is a polymorphic protein that participates in the transformation and metabolism of lipoproteins. Its genes can regulate many biological functions and are related to the pathogenesis of many ophthalmic diseases. Apolipoprotein E is one of the hotspots in medical research at present. To explore the inner relationship between the two has important clinical application value for the prevention, diagnosis and treatment of eye diseases. Apolipoprotein E mainly exists in CM, VLDL, IDL and some In HDL, the concentration of ApoE in normal human plasma is 0.03-0.05g / L, but the existing apolipoprotein E detection kits cannot quantitatively measure the content of apolipoprotein E (APOE) in human serum in vitro, which is not suitable for people's use. bring inconvenience

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