The invention discloses an apolipoprotein E detection kit which comprises a reagent R1, a reagent R2 and a calibration product. The reagent R1 mainly comprises the following components: 10-50mmol/L of phosphate buffer, 10-40g/L of polyethylene glycol, 50-200mmol/L of sodium chloride, 0.02-0.2% of tween 20, 0.1-1% of BSA (Bovine Serum Albumin) and 0.1% of NaN3, wherein polyethylene glycol is low-polymerization polyethylene glycol. The reagent R2 mainly comprises the following components: 10-50mmol/L of phosphate buffer, 50-200mmol/L of sodium chloride, 0.02-0.2% of tween 20, 10-50% (v/v) of ApoE antibody and 0.1% of NaN3; the calibration product mainly comprises the following components: 10-50mmol/L of phosphate buffer, 50-200mmol/L of sodium chloride, 0.1-1% of BSA, 0.2-5mmol/L of EDTA (Ethylene Diamine Tetraacetic Acid), 0.02-0.2% of tween 20, 0.1% of NaN3 and 0-120mg/L of ApoE antigen. The apolipoprotein E detection kit is convenient to use, good in reagent stability, good in detection result accuracy, good in repeatability, convenient to use, low in cost and convenient to popularize and apply.