ApoE gene primer group, detection kit and detection method

A gene detection and primer set technology, applied in the field of DNA detection, can solve problems such as wrong signals in sequencing results, avoid false positives or false negatives, and improve detection accuracy.

Active Publication Date: 2016-08-24
WUHAN CONSIDERIN GENE & HEALTH TECH CO LTD
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Problems solved by technology

[0009] The object of the present invention is to provide a kind of ApoE gene detection primer, detection kit and detection met...

Method used

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  • ApoE gene primer group, detection kit and detection method
  • ApoE gene primer group, detection kit and detection method
  • ApoE gene primer group, detection kit and detection method

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Embodiment Construction

[0051] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0052] In the first typical embodiment of the present invention, an ApoE gene primer set includes a primer set for detecting the rs429358 site and a primer set for the rs7412 site respectively;

[0053] The upstream primer in the primer set for detecting the rs429358 site has a sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4, and the primer set for the detection of the rs429358 site The downstream primer has a sequence of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8;

[0054] The upstream primer in the primer set for detecting rs7412 site has a sequence of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11 or SEQ ID NO: 12, and the primer set for detecting rs7412 site The downstream primer has the sequence of SEQ ID NO:13,...

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Abstract

The invention relates to the technical field of DNA (Deoxyribonucleic Acid) detection, and provides an ApoE (Apolipoprotein E) gene primer group, a detection kit and a detection method, wherein the ApoE gene primer group comprises a primer group for detecting an rs429358 site and a primer group for detecting an rs7412 site; an upstream primer in the primer group for detecting the rs429358 site has any one sequence in SEQ ID NO: 1-4; a downstream primer in the primer group for detecting the rs429358 site has any one sequence in SEQ ID NO: 5-8; an upstream primer in the primer group for detecting the rs7412 site has any one sequence in SEQ ID NO: 9-12; and a downstream primer in the primer group for detecting the rs7412 site has any one sequence in SEQ ID NO: 13-16. The primer group, the kit and the method provided by the invention have the advantage that the correct signal proportion in the ApoE gene detection process can be improved.

Description

technical field [0001] The invention relates to the technical field of DNA detection, in particular to an APOE gene primer set, a detection kit and a detection method. Background technique [0002] The ApoE gene is closely related to a variety of cardiovascular and cerebrovascular diseases. The encoded apolipoprotein E (Apolipoprotein E, ApoE) is involved in the entire occurrence and development of cardiovascular and cerebrovascular diseases such as hyperlipidemia, atherosclerosis, and coronary heart disease. The ApoE gene Polymorphisms are the main cause of individual differences in the early stages and development of these diseases. [0003] According to the ApoE phenotype, the ApoE gene model is proposed, and it is considered that the synthesis of ApoE is controlled by three alleles located at a gene locus, namely E2, E3 and E4, and each allele corresponds to a major isomer. Three homozygotes (E2 / 2, E3 / 3, E4 / 4) and three heterozygotes (E2 / 3, E2 / 4, E3 / 4) have six common p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6869C12Q1/6883C12Q2600/118C12Q2600/156C12Q2563/179C12Q2531/113C12Q2525/191
Inventor 盛司潼邓波
Owner WUHAN CONSIDERIN GENE & HEALTH TECH CO LTD
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