64 results about "Serum plasma" patented technology

The present invention belongs to the field of biological detection technology, and specifically relates to an anti-monkey C-reactive protein monoclonal antibody, an enzyme-linked immunosorbent assay kit and its application. Named CRP-13, CRP-13 includes a heavy chain variable region and a light chain variable region; the heavy chain variable region has three complementary determining regions CDR-H1 to CDR-H3, and the amino acid sequences are shown in SEQ ID NO.6-SEQ ID NO.8; the light chain variable region has three complementary determining regions CDR-L1 to CDR-L3, and the amino acid sequences are shown in SEQ ID NO.9-SEQ ID NO.11. The monoclonal antibody of the present invention can specifically bind to monkey C-reactive protein with high specificity. The above monoclonal antibody can be used to screen a paired monoclonal antibody, and a monkey C-reactive protein enzyme-linked immunosorbent assay kit is obtained based on the combination of the monoclonal antibodies, which can detect the content of monkey C-reactive protein in monkey serum, plasma or tissue fluid.

The invention discloses an LC-MS / MS detection method for a palbozuril monoclonal antibody drug in a blood sample, which comprises the following steps: enriching and purifying the palbozuril monoclonal antibody drug in serum and plasma by coupling an enrichment reagent I with magnetic beads, magnetically separating the enriched blood sample, discarding the supernatant, adding a buffer solution I for leaching, and adding a buffer solution II for diluting after leaching; the method comprises the following steps: carrying out high-temperature denaturation on a diluted blood sample, sequentially adding an internal standard and protease for enzymolysis, and then adopting a high performance liquid chromatography-tandem mass spectrometry to realize high-sensitivity rapid detection of the drug concentration of the palbozuril monoclonal antibody in the blood sample. In the pretreatment process, the steps of protein reduction and alkylation are removed, the proteolysis time is shortened, the whole process is simple, convenient and rapid, and multiple requirements of clinical detection on timeliness, accuracy and low detection cost can be met at the same time.

The invention discloses a preparation method of a CGRP detection kit and application of the CGRP detection kit in migraine diagnosis, and belongs to the technical field of biological medicine detection. According to the invention, alpha-CGRP polypeptide coupled KLH protein is subjected to efficient animal immunization, an antibody with good affinity is screened by using methods such as enzyme-linked immunosorbent assay, cross pairing analysis is carried out, and final antibody sequence information is obtained through sequencing. The paired monoclonal antibody screened by the invention has high sensitivity (KD value of 1nM), the limit of detection (LoD) can be as low as pg / mL level, a low-concentration CGRP sample can be effectively detected, the matrix effect of human serum / plasma can be effectively neutralized by an optimized sample diluent formula, and the repeatability is high (in-plate and inter-plate CV values of 1t and 10%). According to the invention, the kit prepared from the specific paired antibody is systematically and closely linked with the indication function of migraine for the first time, and a clinical diagnosis direction is provided.

The present invention belongs to the field of biological detection technology, and specifically relates to a monkey high-mobility group protein B1 monoclonal antibody, an enzyme-linked immunosorbent assay kit and its application. Named HMGB1-11, it includes a heavy chain variable region and a light chain variable region; the heavy chain variable region has three complementary determining regions CDR-H1 to CDR-H3, and the amino acid sequences are shown in SEQ ID NO.6-SEQ ID NO.8; the light chain variable region has three complementary determining regions CDR-L1 to CDR-L3, and the amino acid sequences are shown in SEQ ID NO.9-SEQ ID NO.11. The monoclonal antibody of the present invention can specifically bind to monkey high-mobility group protein B1, and the monoclonal antibody can be paired with a monoclonal antibody after screening. Based on the monoclonal antibody combination, a monkey high-mobility group protein B1 enzyme-linked immunosorbent assay kit is constructed, which can detect the content of monkey high-mobility group protein B1 in monkey serum, plasma or tissue fluid.

The invention discloses a plasma or serum exosome separation method which comprises the following steps: firstly, coupling an antibody for resisting four transmembrane protein CD81 on the surface of an exosome by using a magnetic bead to form an immunomagnetic bead; pretreating a serum / plasma sample, enriching exosomes, co-incubating with the immunomagnetic beads, washing the magnetic beads after incubation capture is completed, and washing off uncombined impurities; after being captured, the exosome can be directly cracked, the magnetic beads are removed for downstream experiments, or the magnetic beads are eluted to obtain the complete exosome. The exosome affinity antibody is matched with the magnetic beads, the plasma / serum sample exosome is separated, the unique affinity separation technology not only can remove high-abundance protein in the serum / plasma sample, but also can eliminate other vesicles with similar membrane structures with the exosome, so that the purity of the separated exosome is far higher than that of the exosome obtained by other separation methods.

To provide a method for stabilizing a creatinine-containing substance in which, while a calibration substance of creatinine measurement in a sample, a standard substance of creatinine measurement in a sample or a reference substance of accuracy control of creatinine measurement in a sample, or the like that is a creatinine-containing substance to be used in creatinine measurement in a sample, needs to be in a liquid state during use, a concentration of creatinine varies with time in a creatinine-containing substance in the liquid state, and therefore the stability has been inadequate.SOLUTION: In a creatinine-containing substance of a liquid state and a refrigerated condition, in which a solvent is blood serum, blood plasma, or a protein-containing solution, creatine is made to co-exist with creatinine, and further, made to co-exist such that a molar ratio obtained by dividing the creatine concentration (mol / L) by the creatinine concentration (mol / L) falls in the range of 2.0-2.8.SELECTED DRAWING: None

The present invention provides a β-Estradiol antigen epitope, a β-Estradiol antigen, a β-Estradiol specific coating antibody, and a β-Estradiol specific detection antibody. The present invention also provides an ELISA detection kit, which includes the β-Estradiol specific coating antibody, the β-Estradiol specific detection antibody, a standard, Streptavidin-HRP, a single-component TMB colorimetric solution, a stop solution, and a diluent. The kit is suitable for the detection of samples such as animal serum and plasma in scientific research laboratories, and solves the problems of insufficient sensitivity, large intra-batch and inter-batch differences, poor dilution and spike recovery, and difficulty in large-scale industrial production of traditional small molecule β-Estradiol competitive ELISA detection kits.

The application discloses a red blood cell alloantibody specificity analysis method, and aims at the defects in the prior art that a large amount of manual experience and subjective judgment are relied on to exclude positive antigens according to the results of non-agglutination of samples to be detected and cells, and scientific methods such as statistics are lacked to verify the reliability of detection results, and provides a red blood cell alloantibody specificity analysis method. According to the expression of red blood cell antigens of spectrum cells / anti-screen cells and the agglutination results of patient serum / plasma, the red blood cell alloantibody specificity is analyzed by using programmed analysis logic and statistical result analysis.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for NfL protein detection and application. The combination comprises monoclonal antibodies 9E1 and 10B4, and amino acid sequences of complementary determining regions of heavy chain and light chain variable regions of the monoclonal antibodies 9E1 and 10B4 are respectively shown as SEQ ID NO.1-12. The antibody combination can be used for constructing a double-antibody sandwich ELISA detection system and is especially suitable for being combined with a biotin-avidin signal amplification system to achieve high-sensitivity and high-specificity detection of the NfL protein, the detection sensitivity can reach 20 pg / mL, and the method is low in cost, easy and convenient to operate and suitable for quantitative analysis of the NfL protein in samples such as serum and plasma.

The present invention discloses a one-time injection high-efficiency microfluidic detection chip, the detection chip has a rotation center, and the detection chip includes at least one detection unit that can independently perform detection, the detection unit includes a mixed cultivation tank for providing a reaction space, and a solid reagent storage tank storing N different solid reagents is arranged in the mixed cultivation tank; the solid reagent includes a first, a second, ..., and an Nth solid reagent, all of which are coated with a protective film on the outside, and the outside of the Nth solid reagent is sequentially coated with a first protective film, a second protective film, ..., and an Nth protective film from the outside to the inside. The present invention can directly use whole blood, peripheral blood, serum, and plasma samples without sample pretreatment; it can process samples of high hematocrit / polycythemia; only one-time injection of the sample is required to automatically complete complex sample addition and reaction steps; it can reduce the overall reaction time without affecting the detection efficiency, and quickly complete blood testing.

The invention relates to in vitro detection of biomarkers associated with drug-induced liver injury; belongs to the technical field of immunokits. The technical scheme provided by the invention is as follows: the soluble myeloid cell triggered receptor-1 is applied to preparation of a product for diagnosing the drug-induced liver injury as a biomarker. The invention discloses and proves that sTREM1 in serum or plasma can be used as a specific biomarker for diagnosing drug-induced liver injury (DILI), especially drug-induced liver fatty degeneration injury for the first time. The serum / plasma sTREM1 can detect abnormal rise in the extremely early stage (such as 7 days after medication) of the drug-induced liver injury, which is about 5-7 days earlier than that of the traditional marker ALT / AST, so that a key early intervention time window is provided for clinicians, and the liver injury can be prevented from further worsening.

The present invention discloses a method for simultaneously detecting a plurality of fat-soluble vitamins and steroid hormones, comprising the following steps: adding an internal standard to a serum sample, mixing, and then adding methyl tert-butyl ether for extraction; extracting the components to be tested into an organic solvent, centrifuging, drying the extract with nitrogen, re-dissolving, and then injecting and analyzing the extract on a liquid chromatography-mass spectrometry system. The detection method of the present invention can simultaneously detect the contents of five fat-soluble vitamins and seventeen steroid hormones in serum (plasma), and simultaneously test a total of 22 substances to be tested, which is accurate and can be quantitatively detected. The present invention adopts a scheme that changes the conventional prior art of using the isotope of the substance to be tested as the internal standard substance, and adopts trenbolone cyclohexylmethyl carbonate as the internal standard, which can simultaneously quantify 22 substances to be tested, greatly reducing the workload.

The invention belongs to the technical field of biology, and discloses a sample diluent for ELISA (Enzyme-Linked Immunosorbent Assay), application of the sample diluent and a kit. The sample diluent comprises the following components: sodium chloride, sodium dodecyl sarcosinate and sodium caseinate, the mass ratio of the sodium chloride to the sodium dodecyl sarcosinate to the sodium caseinate is (1.6-6.4): (0.01-0.1): (0.5-2); according to the sample diluent, the synergistic effect of sodium chloride, sodium dodecyl sarcosinate and sodium caseinate with different concentrations is utilized, and matrix interference in animal serum / plasma can be remarkably reduced, so that the sensitivity and specificity of an ELISA kit are improved.

The present invention belongs to the field of biological detection technology, and specifically relates to an anti-monkey interleukin-6 monoclonal antibody, an enzyme-linked immunosorbent assay kit and its application. Named IL-6-12, IL-6-12 includes a heavy chain variable region and a light chain variable region; the heavy chain variable region has three complementary determining regions CDR-H1 to CDR-H3, and the amino acid sequence is shown in SEQ ID NO.6-SEQ ID NO.8; the light chain variable region has three complementary determining regions CDR-L1 to CDR-L3, and the amino acid sequence is shown in SEQ ID NO.9-SEQ ID NO.11. The monoclonal antibody of the present invention can specifically bind to monkey interleukin-6, and the above-mentioned monoclonal antibody is used to screen a pairable monoclonal antibody. Based on the combination of the monoclonal antibodies, a monkey interleukin-6 enzyme-linked immunosorbent assay kit is obtained, which can detect the content of monkey interleukin-6 in monkey serum, plasma or tissue fluid.

The invention discloses an erythrocyte isoantibody specificity analysis method, and aims to overcome the defects that in the prior art, when positive antigens are excluded according to non-agglutination results of a sample to be detected and cells, a large amount of artificial experience and subjective judgment are relied on, and scientific methods such as statistics are lacked to verify the credibility of the detection results. The invention provides an erythrocyte isoantibody specificity analysis method. According to erythrocyte antigen expression of spectrum cells / anti-screening cells and patient serum / plasma agglutination results, erythrocyte isoantibody specificity is analyzed by using programmed analysis logic and statistical results.

A method for the extraction and enrichment of Hepatitis C Virus (HCV) antigens by conjugating specific antibodies targeting Envelope 1, Envelope 2, NS3, and NS4 antigens to a temperature-responsive smart polymer (NIPAAm-Co-HIPAAm-Co-SAKIPAAm). The application also relates to a detection kit for HCV antigens, comprising the smart polymer conjugated to specific antibodies, colloidal gold nanoparticles conjugated to specific antibodies, and instructions for detecting HCV antigens in serum, plasma, or whole blood samples.

The invention relates to the technical field of adiponectin detection kits, and discloses an immunoturbidimetric kit for detecting ADP, wherein the kit comprises an R1 reagent and an R2 reagent; the R1 reagent comprises: a buffer solution, 10-100 mmol / L; NaCl, 0.1-1 mol / L; an anticoagulant, 10-100 mmol / L; an aggregation promoter, 0.1-1%; a surfactant, 0.1-1 g / L; a preservative, 0.5-1 g / L; and a blocking agent, 0.05-0.2 mg / mL; wherein the anticoagulant in the R1 reagent is EDTA-Na 2 , sodium heparin, sodium citrate or a combination thereof; R2 reagent: buffer and antibody-coated latex microspheres, the preparation process of which requires cross-linking liquid, blocking liquid and preservation liquid; the kit prepared by the present invention achieves the beneficial technical effects of reducing the difference in serum and plasma measurement values, reducing batch differences and improving stability.

The application discloses application of a reagent for detecting ILA concentration in preparation of a product for predicting the curative effect of esophageal squamous cell carcinoma immunotherapy, a prediction method and equipment, and relates to the technical field of immunotherapy curative effect prediction. The reagent for detecting indole-3-lactic acid concentration is aimed at samples selected from serum, plasma, blood, tumor tissue or fecal samples. By establishing a prediction model for the curative effect of esophageal squamous cell carcinoma immunotherapy, the curative effect of immunotherapy on esophageal squamous cell carcinoma patients can be accurately predicted, valuable reference bases can be provided for clinicians, thus helping to formulate individualized immunotherapy strategies and improving the curative effect of immunotherapy.

The application provides a detection method of small molecule substances. The detection method comprises the following steps: mixing, incubating and mixing a sample, a diluent and a ligand, the small molecule substances in the sample are specifically combined with the ligand to form an immune complex, a complex antibody labeled with a signal generator is added, the complex antibody is specifically combined with a new conformation formed by the small molecule substances and the ligand to form a ternary immune complex, and the content of the ternary immune complex is detected; the molecular weight of the small molecule substance to be detected is less than 2000D; the diluent comprises a metal chelate and a buffer; the metal chelate comprises EDTA-2K or EDTA-2Na; the sample is a serum sample or a plasma sample, and the method solves the problem of detection difference between serum and plasma blood collection tube samples in the existing small molecule detection field.

The present application relates to the field of rapid diagnostic technology, and more specifically, discloses an antigen detection test strip and a preparation method thereof; the test strip comprises a base plate and a sample pad, a blank pad, a nitrocellulose membrane and a water-absorbing pad sequentially overlapped on the base plate in the chromatography direction; the nitrocellulose membrane is provided with a marking area, a detection line and a quality control line; the marking area, the detection line and the quality control line are sequentially located on the nitrocellulose membrane along the chromatography direction; the detection line is coated with a detection antibody, and the quality control line is coated with a quality control antigen or antibody; an NC membrane treatment component and a quantum dot conjugate are sequentially fixed on the marking area, and the quantum dot conjugate is superimposed on the NC membrane treatment component. The present application can achieve rapid release of the labeled conjugate, and can be detected using a small amount of peripheral blood / venous whole blood / serum / plasma sample, and a report can be issued within 5 minutes. It has the advantages of being easy to use, simple to operate and easy to promote.

The present invention belongs to the field of biological detection technology, and specifically relates to an anti-monkey interleukin-6 monoclonal antibody, an enzyme-linked immunosorbent assay kit and its application. Named IL-6-12, IL-6-12 includes a heavy chain variable region and a light chain variable region; the heavy chain variable region has three complementary determining regions CDR-H1 to CDR-H3, and the amino acid sequence is shown in SEQ ID NO.6-SEQ ID NO.8; the light chain variable region has three complementary determining regions CDR-L1 to CDR-L3, and the amino acid sequence is shown in SEQ ID NO.9-SEQ ID NO.11. The monoclonal antibody of the present invention can specifically bind to monkey interleukin-6, and the above-mentioned monoclonal antibody is used to screen a pairable monoclonal antibody. Based on the combination of the monoclonal antibodies, a monkey interleukin-6 enzyme-linked immunosorbent assay kit is obtained, which can detect the content of monkey interleukin-6 in monkey serum, plasma or tissue fluid.

1. The name of the product of this design: Handheld blood lipid analyzer. 2. Purpose of the product with this appearance design: used in conjunction with the matching test card, it adopts reflectance photometry to quantitatively analyze the clinical chemical components in whole blood, serum, plasma and peripheral blood of human blood samples. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The utility model provides a reagent card for whole blood separation liquid phase test, which comprises a card body, a separation cavity and an extraction cavity are arranged on the card body, a liquid outlet is arranged at the bottom of the separation cavity, and the liquid outlet is communicated with the extraction cavity; a filter membrane covering the liquid outlet is fixed in the separation cavity; a supercharging device is arranged in the separation cavity and used for increasing the air pressure in the separation cavity. A whole blood separation structure is arranged on the reagent card, when blood plasma or serum needs to be detected, collected whole blood is added into the separation cavity, the pressurizing device increases the air pressure in the separation cavity, the whole blood permeates the filtering membrane under the dual action of gravity and the air pressure to obtain the blood plasma or serum, the blood plasma or serum flows into the extraction cavity through the liquid outlet, and then the blood plasma or serum is extracted. A centrifugal device or a filtering device does not need to be additionally adopted for treating whole blood, and the operation convenience is improved.

The application discloses a platelet antibody detection and cross matching blocking chromatography kit and a detection method thereof, wherein the kit comprises a U-shaped microwell plate, a chromatography medium for separating platelet immune complexes from non-cell particle components under the action of centrifugal force, freeze-dried platelets, a platelet diluent, a platelet fluorescent staining solution comprising fluoresceinized platelet antibodies specifically labeled for platelets, biotin-labeled anti-human globulin for specifically binding with antibodies bound to the surface of the platelets, avidinized fluorescein for coupling with the biotin-labeled anti-human globulin through a biotin-avidin specific binding system, negative control serum / plasma and positive control serum / plasma. The application combines the blocking chromatography technology with a high-sensitivity flow immunofluorescence method to construct an efficient and accurate platelet antibody detection and cross matching system, which is high in specificity and sensitivity, simple in operation and stable in detection results.

The invention relates to the technical field of medical examination, and provides a detection tube for serum / plasma insufficiency samples, which comprises a blood collection tube, the top of the blood collection tube is fixedly connected with a tube opening convex edge, the top end of the blood collection tube is provided with a detection tube fitting, the detection tube fitting comprises a lower tube and an upper tube, the inner side of the upper tube is provided with a clamping groove in sliding fit with the tube opening convex edge, and the upper tube is provided with a clamping groove. An aluminum film adheres to the top of the upper-section pipe, a liquid pumping hose is fixedly connected to the bottom of the lower-section pipe, a one-way valve is fixedly connected to the liquid pumping hose, and a negative pressure piece which abuts against the pipe opening convex edge to pump out gas in the upper-section pipe to enable the upper-section pipe to generate negative pressure is arranged on the inner side of the clamping groove. A plurality of positioning pieces which are in sliding fit with the outer wall of the convex edge of the pipe orifice are arranged on the outer side of the upper-section pipe and are distributed around the upper-section pipe at equal angles. Through intelligent design and component optimization, the detection feasibility of the serum / plasma insufficiency sample is remarkably improved, and the progress of the medical examination technology is promoted.

This invention provides a material for detecting serum plasma anti-nicotinic acetylcholine receptor antibodies and its method of use. The material comprises HEK293T cells transfected with three recombinant expression plasmids: the first plasmid encodes a truncated AChR α subunit; the second plasmid is a polycistronic plasmid encoding a fusion protein composed of a truncated AChR β subunit, an AChR δ subunit, and rapsyn protein linked by a P2A peptide; and the third plasmid encodes a truncated AChR γ subunit or ε subunit. After co-transfection with PEI, the cells are cultured in fresh complete medium for 25-35 hours after 7-9 hours post-transfection, followed by fixation, blocking, and preservation. This invention, through a three-plasmid system and antigen truncation modification, reduces cytotoxicity while ensuring high expression of the functional pentamer, significantly improving detection sensitivity and reagent shelf life.

The invention discloses application of a reagent for detecting ILA concentration in preparation of a product for predicting esophageal squamous cell carcinoma immunotherapy curative effect, a prediction method and equipment, and relates to the technical field of immunotherapy curative effect prediction. A sample targeted by the reagent for detecting the concentration of indole-3-lactic acid is selected from serum, plasma, blood, tumor tissue or a fecal sample. By establishing the prediction model for the curative effect of the esophageal squamous cell carcinoma immunotherapy, the immunotherapy effect of a patient with the esophageal squamous cell carcinoma can be accurately predicted, and valuable reference bases can be provided for clinicians, so that making of personalized immunotherapy strategies is facilitated, and the effect of the immunotherapy is improved.

According to the rapid detection colloidal gold test strip for the SFTSV antigen and the preparation method of the rapid detection colloidal gold test strip, the SFTSVNP antigen is detected by adopting a double-antibody sandwich method, an N protein monoclonal antibody 1G, prepared in a laboratory, of a human-derived Dualtada virus is used as a detection antibody, and an N protein monoclonal antibody 1E, labeled by colloidal gold, of the human-derived Dualtada virus is used as a labeled antibody; the test strip is formed by sequentially adhering a sample pad, a colloidal gold pad, a cellulose acetate membrane (NC membrane) and absorbent paper to a PVC (polyvinyl chloride) bottom plate along the chromatography direction; the NC membrane is sequentially coated with a detection line and a quality control line along the chromatography direction; the prepared rapid detection colloidal gold test strip for the SFTSV antigen can qualitatively detect the Databean virus in peripheral blood, serum and plasma samples, and a rapid and reliable virus detection technology suitable for grassroots units and field application is established.

An automatic extraction device for large-volume free nucleic acid through a centrifugal column method comprises a back plate, a shifting mechanism and a reagent filling module, the shifting mechanism and the reagent filling module are sequentially and fixedly arranged on the back plate, and a centrifugal column filtering module with a negative-pressure liquid pumping module is arranged at the bottom of the reagent filling module. Through a centrifugal column specifically combined with nucleic acid and a special reagent filling module, after a sample is subjected to cracking and protease K digestion treatment, nucleic acid in the sample is rapidly adsorbed and rapidly washed through a filter membrane, so that protein, pigments, lipids and other impurities are removed to the maximum extent; the obtained total nucleic acid can be stored in an eluent for a long time to be used for a next experiment. The whole device can realize centrifugal column method large-volume free nucleic acid automatic extraction for extracting, enriching and purifying the free total nucleic acid from fresh or frozen serum, plasma, lymph and other cell-free body fluids.

1. Name of the Design Product: Automatic Chemiluminescence Immunoassay Analyzer (AE-200). 2. Use of the Design Product: The product is based on the direct chemiluminescence method of acridinium ester and is used together with detection reagents for qualitative or quantitative detection of analytes in serum, plasma, and urine samples derived from the human body clinically. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Front View.