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Antibody diluent for enzyme-linked immunoassay and preparation method thereof

An enzyme-linked immunosorbent assay, antibody diluent technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of antibody storage time limitation, and achieve the effects of prolonged storage time, system optimization, and reasonable composition

Pending Publication Date: 2020-04-28
WUHAN KANGZHU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides an antibody diluent for enzyme-linked immunoassay detection and a preparation method thereof, which solves the problem that in the prior art, the antibody diluent composition of serum plasma and peripheral blood is relatively simple, and the antibody preservation after dilution is relatively simple. Time is limited to a certain extent, and there are certain limitations

Method used

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Examples

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Embodiment 1

[0026] The embodiment of the present invention provides an antibody diluent for enzyme-linked immunoassay detection, wherein the diluent is composed of the following components in parts by weight: 80 parts of 0.01M PBS buffer solution, 0.5 part of bovine serum albumin, 0.6 part of cold fish skin gel, three 0.1 part of sodium nitride, 0.3 part of Proclin300 preservative, 0.2 part of sodium lactate, 0.4 part of gentamicin sulfate, and 1 part of anticoagulant.

[0027] The 0.01M PBS buffer solution is composed of potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20 and deionized water, of which potassium dihydrogen phosphate, disodium hydrogen phosphate, chloride The mass ratio of sodium to potassium chloride is 0.2-0.3:1.2-1.6:7-9:0.15-0.25, and the anticoagulant is one of hirudin, sodium citrate and EDTA.

[0028] The antibody diluent preparation method for ELISA comprises the following preparation steps:

[0029] S1, prepa...

Embodiment 2

[0036] The embodiment of the present invention provides an antibody diluent for enzyme-linked immunoassay detection, wherein the diluent is composed of the following components in parts by weight: 85 parts of 0.01M PBS buffer solution, 1 part of bovine serum albumin, 0.9 part of cold fish skin gel, three 0.15 parts of sodium nitride, 0.4 parts of Proclin300 preservative, 0.3 parts of sodium lactate, 0.5 parts of gentamicin sulfate, and 1.5 parts of anticoagulant.

[0037] The 0.01M PBS buffer solution is composed of potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20 and deionized water, of which potassium dihydrogen phosphate, disodium hydrogen phosphate, chloride The mass ratio of sodium to potassium chloride is 0.2-0.3:1.2-1.6:7-9:0.15-0.25, and the anticoagulant is one of hirudin, sodium citrate and EDTA.

[0038] The antibody diluent preparation method for ELISA comprises the following preparation steps:

[0039] S1,...

Embodiment 3

[0046] The embodiment of the present invention provides an antibody diluent for ELISA, wherein the diluent is composed of the following components in parts by weight: 90 parts of 0.01M PBS buffer, 1.5 parts of bovine serum albumin, 1.2 parts of cold fish skin gel, three 0.2 parts of sodium nitride, 0.5 parts of Proclin300 preservative, 0.4 parts of sodium lactate, 0.6 parts of gentamicin sulfate, and 2 parts of anticoagulant.

[0047] The 0.01M PBS buffer solution is composed of potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20 and deionized water, of which potassium dihydrogen phosphate, disodium hydrogen phosphate, chloride The mass ratio of sodium to potassium chloride is 0.2-0.3:1.2-1.6:7-9:0.15-0.25, and the anticoagulant is one of hirudin, sodium citrate and EDTA.

[0048]The antibody diluent preparation method for ELISA comprises the following preparation steps:

[0049] S1, prepare 0.01M PBS buffer solution, the...

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Abstract

The invention provides an antibody diluent for enzyme-linked immunoassay and a preparation method of the antibody diluent, and relates to the technical field of enzyme-linked immunoassay. The antibodydiluent for enzyme-linked immunoassay comprises the following components, by weight, 80 to 90 parts of a 0.01 M PBS buffer solution, 0.5-1.5 parts of bovine serum albumin, 0.6-1.2 parts of cold fishskin gel, 0.1-0.2 parts of sodium trinitride, 0.3-0.5 parts of a Proclin 300 preservative, 0.2-0.4 parts of sodium lactate, 0.4-0.6 parts of gentamicin sulfate and 1-2 parts of an anticoagulant, wherein the 0.01 M PBS buffer solution is prepared from monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, tween-20 and deionized water. In the invention, preparation raw materials are reasonably used, and a system of the antibody diluent is optimized so that components of the antibody diluent of serum and plasma and peripheral blood are more reasonable, preservation time of the diluted antibody is effectively prolonged, and the antibody diluent is beneficial to medical use.

Description

technical field [0001] The invention relates to the technical field of enzyme-linked immunoassay, in particular to an antibody diluent for enzyme-linked immunoassay and a preparation method thereof. Background technique [0002] The whole process of enzyme-linked immunoassay method includes the separation and purification of virus antigen, the preparation of virus antiserum and antiserum reaction. The corresponding enzyme-labeled antibody generates a complex of antigen-antibody to be tested-enzyme-labeled antibody, and then reacts with the substrate of the enzyme to generate a colored product. The amount of antibody was calculated by means of light absorbance in a spectrophotometer. The amount of antibody to be tested is directly proportional to the colored product. In the same way, antibodies can also be coated to measure the antigen content. ELISA is commonly used for the determination of antigen by direct method, the determination of antigen by competition method, and ...

Claims

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Application Information

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IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 张无量
Owner WUHAN KANGZHU BIOTECH
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