52 results about "Gentamicin Sulfate" patented technology

A hepatitis c virus antigen-antibody joint detection reagent box is characterized by comprising a calibrator(1), a double-marker enzyme conjugate (2), a negative and positive contrast (3), light-emitting liquid (4) and a micropore coated plate (5), wherein the light-emitting liquid contains light-emitting liquid 1 and light-emitting liquid 2, the light-emitting liquid 1 contains luminal 0.7 g/L, cinnamic acid 0.9 g/L, 4-iodophenylboronic acid 0.2 g/L, iodobiphenol 0.25 g/L, dimethylformamide 25 ml/L, polyving akohol 5 g/L, polyvinylpyrrolidone 8 g/L, polyethylene glycol 600 3 g/L, ethylenediamine tetraacetic acid 4 g/L, gentamicin sulfate 1600 thousands/L, urea peroxide 0.4 g/L, and pH 9.0 Tris buffer solution 0.1 mol/L. The light-emitting liquid 2 contains acridinium ester derivative 0.1 mg/ml, polyethylene glycol 600 3 g/L and 0.1 mol/L of pH 9.0 Tris buffer solution containing 0.1% of TWEEN-20. The invention further discloses a preparation method and a using method of the reagent box. The hepatitis c virus antigen-antibody joint detection reagent box has the advantages of being quick in reaction and low in cost.

The invention discloses a gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and a preparation method, belonging to the technical field of high molecular materials. The preparation method comprises the following steps: dropwise adding gentamicin sulfate solution with concentration of 10-50 mg/ml into gelatin microspheres, placing overnight at the temperature of 4-25 DEG C and then performing freeze drying to obtain a gentamicin sulfate/gelatin microsphere complex; adding the gentamicin sulfate/gelatin microsphere complex into 2-10 percent by weight of silk fibroin solution, uniformly stirring and then performing freeze drying to obtain the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin porous composite scaffold, wherein the porosity of the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold is 75-95 percent, the aperture of the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold is 50-200 mum, and the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold has long-acting antibacterial and drug slow-release functions and can be applied to the fields of skin wound healing and wound dressing.

The invention discloses a natural compound feed additive for removing veterinary drug residues. The natural compound feed additive is prepared from the following components in parts by weight: 1 part of procyanidine, 1 part of astaxanthin, 1 part of resveratrol, 1 part of curcumin, 1 part of allicin, 1 part of porphyra polysaccharide, 1 part of porphyra polyphenol, 1 part of phycobiliprotein, 1 part of tea polyphenol, 1 part of quercetin, 1 part of chitosan and 1 part of tangeretin; and the ratio of the feed additive to the feed is (1:100) to (1:500) in use. According to the method, the residues of tilmicosin, florfenicol, ceftiofur sodium, thiabendazole, enrofloxacin, gentamicin sulfate, oxytetracycline, sulfadimethoxine and halofuginone hydrobromide in livestock or poultry bodies can be effectively reduced; the natural compound feed additive has the characteristics of being free of pollution and free of public hazard, and can be applied to removal of veterinary drug residues from food animals of which the veterinary drug residues exceed the standard due the factors such as veterinary drug application and environmental pollution, so as to ensure the hygiene and safety of animal food.

The invention relates to a gentamicin sulfate wrapped in polylactic acid/nano-hydroxyapatite composite microspheres, which comprises the following components in parts by weight: 1-10 parts of nano-hydroxyapatite, 1 part of gentamicin sulfate, 12.5-25 parts of polylactic acid and 1-17 parts of methyl cellulose. The invention further discloses a preparation method. The prepared gentamicin sulfate has the advantages of high drug loading, high entrapment efficiency, long-term sustained release and biodegradability.

The invention discloses corneal metaphase preserving liquid containing recombinant human serum albumin and a preparation method thereof. The corneal metaphase preserving liquid containing recombinanthuman serum albumin contains glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal.HCl, riboflavin, thiamine.HCl, KCl, NaCl, NaH2PO4.2H2O, glucose, dextran 40, sodium pyruvate, vitamin C, chondroitin sulphate sodium, sodium hyaluronate, non-animal recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, piperazine-1-erhanesulfonic acid, phenol red indicator, and water for injection. The non-animal recombinant human serum albumin is added to replace serum to improve corneal endothelial cell survival rate; and then the non-animal recombinant human serum albumin cooperates with a compound system of the chondroitin sulphate sodium, the sodium hyaluronate and the dextran 40, the survival rate of the corneal endothelial cell is greatly ensured.

The invention belongs to the technical field of animal cell engineering and particularly relates to a disinfectant for in vitro cell culture of animals and a preparation method of the disinfectant. The compound disinfectant is mainly prepared from penicillin, streptomycin sulfate, gentamicin sulfate and amphotericin B. Under the condition that cell survival is not affected, the compound disinfectant provided by the invention has a comprehensive antimicrobial spectrum, can simultaneously inhibit the growth of most of Gram-positive bacteria, Gram-negative bacteria and molds, realizes primary culture and tissue sample disinfection of animal cells, and prevents microbial contamination in cell passage processes.

The invention discloses a preparation method of gentamicin sulfate capable of reducing impurities, which can significantly reduce the impurities of gentamicin sulfate products, meets the requirements of "Chinese Pharmacopoeia" (2015 edition), and the production process is simple and easy to operate; The present invention differs from the prior art in the following three points: 0.001% adenosine triphosphate and 0.001% glycine are added to the spore slant culture medium; 0.001% adenosine triphosphate and 0.001% glycine are added to the seed culture medium; 0.003% adenosine triphosphate is added to the fermentation medium and 0.003% glycine; the total miscellaneous content of the gentamicin sulfate product produced before the improvement > 4%, and sisomicin > 1.5%; Somicin <1.5%, Minor Normycin (Small Normicin) <3%.

The invention provides diluting powder for artificial fertilization of pigs. The diluting powder is prepared by mixing the following raw materials in parts by weight: 27-28 parts of glucose, 6.5-7.5 parts of sodium citrate, 0.5-1.5 parts of sodium bicarbonate, 2-3 parts of ethylene diamine tetraacetic acid disodium, 2.5-3.5 parts of anhydrous citric acid, 5-6 parts of tris(hydroxymethyl)aminomethane, 0.1 part of gentamicin sulfate, 0.1g of kanamycin and 1000 parts of distilled water. The diluting powder has low cost and good effects.

The invention discloses a method for proliferating natural killer cells. The method comprises the following steps: (1) separating lymphocytes from whole blood; inoculating the lymphocytes in a first culture medium for first proliferation culture to obtain a first proliferation culture product; (2) mixing the first proliferation culture product with a second culture medium according to a volume ratio of 1 to (0.5-2) and performing second proliferation culture to obtain a second proliferation culture product; (3) replacing the first proliferation culture product with the second proliferation culture product, and repeatedly operating according to step (2) for 2-7 times. The invention also provides a culture medium composition. The culture medium composition contains a basal culture medium, L-glutamine, gentamicin sulfate, mannatide, interleukin 2 and interleukin 15. The proliferation method disclosed by the invention is a natural killer cell proliferation method which is simple, convenient, high in efficiency, easy to operate and relatively high in safety.

The invention discloses an external medicine for treating psoriasis. The external medicine for treating psoriasis is in a cream type, and is characterized by being prepared from the following components in parts: 30 to 42 parts of centipede, 20 to 25 parts of scorpion, 5 to 7 parts of borneol, 400 to 600 parts of vaseline, and 30 to 40 parts of gentamicin sulfate. The invention also provides a medicine composition for treating the psoriasis.

The invention discloses a novel medicament for treating bacterial septicemia of freshwater fish. The novel medicament comprises the following components in parts by weight: 30-60 parts of gentamicin sulfate for oral administration, 1-3 parts of vitamin E, 0.2-0.4 part of vitamin C, 0.3-0.6 part of glycine, 0.2-0.4 part of tyrosine, 1-3 parts of enrofloxacin, and 300-600 parts of water. Compared with the prior art, the novel medicament has the beneficial effects of excellent usage effect, simple preparation technology, low cost and the like.

The invention discloses a method for measuring histamine content in gentamicin sulfate for veterinary use, and belongs to the technical field of analytical chemistry. The measuring method uses hydrochloric acid-methanol solution or hydrochloric acid-acetonitrile solution to extract histamine of diluted gentamicin sulfate for veterinary use, purifies impurities in the extracting solution with C18 powder, re-dissolves by nitrogen blowing and acetonitrile formic acid solution, and then performs LC-MS/MS determination. The method for measuring histamine content in gentamicin sulfate for veterinaryuse provided by the invention enables the HPLC mass spectrometer to better exclude matrix interferences by pre-processing the sample, and then uses LC-MS/MS to quickly and accurately perform detection and quantification of histamine in gentamicin sulfate for veterinary use, which overcomes the difficulty in extracting histamine in gentamicin sulfate for veterinary use, and makes up for the inability to quantitative detection of histamine content in gentamicin sulfate for veterinary use in the prior art.

The invention relates to the technical field of tissue culture, and discloses a culture medium for preparing endometrial stem cells as well as a preparation method. On the basis of a serum-free culture medium, Pall serum substitute, penicillin/streptomycin, gentamicin sulfate, glutamine, MEM NEAA, EGF, bEGF, sodium pyruvate and VEGF are added as nutritional ingredients of the endometrial stem cells, so that the survival rate of the endometrial stem cells can be significantly improved; and by combining with the mixed digestion of collagenase I and Dispase II, the survival rate of the endometrial stem cells can be further improved, and the development application of the endometrial stem cells can be promoted.

The invention claims to protect gentamicin sulfate prepared through enhanced microbial fermentation, and an application method of the gentamicin sulfate. The enhanced microbial engineering bacterium is a Micromonospora purpurea C2 1591 strain which is obtained by screening through a normal-pressure room-temperature plasma mutagenesis technology, and the Micromonospora purpurea C2 1591 strain is registered and collected in the China General Microbiological Culture Collection Center (CGMCC for short, the address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing) on June 13, 2021, and the collection number is CGMCC No.59450. The screened engineering bacterium for directionally producing gentamicin C2 in high yield can directionally produce the gentamicin C2 in the high yield, the component ratio of gentamicin C2 is increased to 85% or above from the original about 15%, and the highest fermentation titer of an optimized formula is 1184 U/mL through Plackett-Burman design and is increased by 42% than the titer of an original formula. Compared with an original strain, the yield of the gentamicin C2 is increased by three times, in addition, other isomers or impurities are hardly contained, the gentamicin C2 can be directly applied downstream, complex biotransformation and purification is not required, a large amount of cost is saved, and an industrialization requirement is met.

The invention discloses a sample diluent applicable to peripheral blood detection. The sample diluent per 1,000ml is prepared from the ingredients: 20.5g of glucose, 8.5g of sodium chloride, 2g of bovine serum albumin, 0.55g of citric acid, 0.4ml of Proclin 300 and 2ml of gentamicin sulfate. The sample diluent is readily available in required raw materials, low in price and free of blood serum ingredient, the risk of infecting some special viruses is avoided, the osmotic pressure of solutions inside and outside cells is balanced by various ions, and the preservation effect is remarkable. The optimized sample diluent is high in applicability, wide in range of application and superior in oxidation resistance and can exert a protecting action during the transportation and preservation of peripheral blood diluted samples.

The invention discloses a quality control product suitable for quantitative detection of a tumor marker Pro-GRP, comprising the following steps of: first, selecting a de-hormone serum suitable for thetumor marker Pro-GRP, and then storing at 2-8 DEG C after thawing; successively adding 0.1-0.5% ADP, Proclin300, gentamicin sulfate, and 1%-10% trehalose to an matrix solution and storing at 2-8 DEGC; adding a tumor marker Pro-GRP antigen into the matrix solution, and then adding lauryl sodium sulfate to obtain the quality control product of a liquid tumor marker Pro-GRP; and dispensing the product in a brown glass bottle, obtaining a finished product of the quality control product of the tumor marker Pro-GRP after performing vacuum freeze drying, and storing the finished product in a refrigerator at 2-8 DEG C. The quality control product of the tumor marker Pro-GRP prepared by the invention has good uniformity and stability, is not affected by transportation, temperature and the like, and can be applied to quantitative detections of different instrument models and different reagents. The quality control product is a lyophilized product, and has reliable quality and high accuracy. The raw materials for preparing the quality control product are widely used, the preparation method is simple, and the cost is low.

The invention discloses sperm refrigerating fluid for human assisted reproduction and a preparation method thereof. The cane sugar in the sperm refrigerating fluid is replaced trehalose. The sperm refrigerating fluid comprises 95.00-105.00 mmol/L of sodium chloride, 3.45-6.55 mmol/L of potassium chloride, 0.55-0.65 mmol/L of magnesium sulfate, 1.75-2.22 mmol/L of calcium chloride, 0.18-0.39 mmol/Lof sodium dihydrogen phosphate, 0.58-1.78 mmol/L of sodium citrate, 3.85-5.45mmol/L of sodium bicarbonate, 15.00-25.00mmol/L of HEPES and MOPS, 0.38-0.68 mmol/L of glucose, 0.28-0.42 mmol/L of sodium pyruvate, 12.48-23.68 mmol/L of sodium lactate, 5-15 mg/mL of human serum albumin, 7-14 [mu]g/mL of gentamicin sulfate, 25-35% (v/v) of glycerin, and 0.15-0.65 mol/L of trehalose. The preparation method comprises the following steps: weighing various components, dissolving the components in injection-grade water, performing filtering and sterilizing by using a 0.2 [mu]m filter membrane, performing sampling and testing, wherein the pH value is controlled to be 7.00-7.40, the osmotic pressure ranges from 1720 mOsm/Kg to 2020 mOsm/Kg, the bacterial endotoxin is less than 0.25 EU/ml, and the blastocyst formation rate is greater than or equal to 80% when 1 cell mouse embryo is cultured for 96h.

The invention provides a skin mucous membrane damage antibacterial disinfectant and a preparation method thereof. The skin mucous membrane damage antibacterial disinfectant is mainly prepared from vitamin 12 and gentamicin sulfate, is capable of effectively killing various bacteria and harmful microorganisms of skin mucous membranes, is applied to antibacterial disinfection on radioactive and non-radioactive mucous membranes, is also applicable to antibacterial sterilization and disinfection for normal and damaged skin, mucous membrane tissue, hygiene and disease control and the like, and hasfunctions of disinfection and bacterial resistance on radioactive combined injury. The disinfectant can be a spray, powder, a solution, gel and an ointment associated with medical preparations, and can be also made into sanitary products such as lotions, wet tissue paper, facial masks, mouth-muffles, filming agents and toothpaste products.

The invention relates to a compound lincomycin hydrochloride injection for livestock. The compound lincomycin hydrochloride injection is characterized in that the compound lincomycin hydrochloride injection is prepared through evenly mixing the following compositions in weight portion: 8 to 20 portions of lincomycin hydrochloride, 5 to 20 portions of berberine bisulfate, 5 to 15 portions of gentamicin sulfate-micronomicin sulfate and 45 to 100 portions of water for injection. The preparation method comprises the following steps: (1) according to weight portion, 8 to 20 portions of the lincomycin hydrochloride, 5 to 20 portions of the berberine bisulfate and 5 to 15 portions of the gentamicin sulfate-micronomicin sulfate are weighed; (2) the lincomycin hydrochloride is put into 30 to 40 portions of the water for injection at a temperature of between 45 and 55 DEG C and is stirred for 10 to 25 minutes to clarification; (3) 10 to 20 portions of the water for injection is taken out, is sequentially added with 5 to 15 portions of the gentamicin sulfate-micronomicin sulfate and 5 to 20 portions of the berberine bisulfate and is stirred and dissolved; and (4) a solution prepared in the step 2 and a solution prepared in the step 3 are mixed, are evenly stirred and are kept for 15 to 20 minutes; and after a medicine solution is clarified, the residual part of the water for injection is added into the medicine solution and is evenly stirred and filtered. The compound lincomycin hydrochloride injection has a reasonable formula, simple preparation and remarkable curative effect.

The tablet for treating gastrilis features that genetamiycin sulfate, novocainum and vitamin B12 are compounded into compound preparation, and each tablet contains gentamicin sulfate 20000-4000 IU, novocainum 0.1-0.2g and vitamin B12 50-100 mirograms. During the preparation, starh and carboxymethyl starch are used as pelletizing supplementary materials, magnesium stearace is used as tabletting flow assistant, and gastro-soluble acrylic resin and hydroxyproyl cellulose are used as main supplementary materials for quick gastro-soluble membranous. The tablet of the present invention has determinate curative effect.