Sperm refrigerating fluid for human assisted reproduction and preparation method thereof

A technology for assisted reproduction and cryotherapy, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve problems such as destroying sperm cells.

Inactive Publication Date: 2020-05-29
东蕴医疗科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the thawing process, about half of the sperm cells will die. There are many reasons. For example, when the ice crystals in the sperm cells mel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of sperm freezing liquid for human assisted reproduction

[0031] The saline solution is a compound culture solution using HEPES and MOPS as buffers, based on sodium, potassium, magnesium, and calcium ions, and using trehalose, glucose, sodium pyruvate, sodium lactate, and human serum albumin as energy substances; It contains: sodium chloride 95.45mmol / L, potassium chloride 3.85mmol / L, magnesium sulfate 0.65mmol / L, calcium chloride 2.12mmol / L, sodium dihydrogen phosphate 0.28mmol / L, sodium citrate 1.65mmol / L , sodium bicarbonate 5.25mmol / L, HEPES and MOPS 25.00mmol / L, glucose 0.46mmol / L, sodium pyruvate 0.32mmol / L, sodium lactate 14.45mmol / L, human serum albumin 13mg / mL, trehalose 0.55mol / L L, gentamicin sulfate 12ug / mL.

[0032] Concrete preparation method comprises the following steps:

[0033] (1) Weigh the various components and set aside;

[0034] (2) Preparation of saline solution: Among the weighed components, first dissolve all components ...

Embodiment 2

[0045] Embodiment 2 A kind of sperm freezing liquid for human assisted reproduction

[0046]The saline solution is a compound culture solution using HEPES and MOPS as buffers, based on sodium, potassium, magnesium, and calcium ions, and using trehalose, glucose, sodium pyruvate, sodium lactate, and human serum albumin as energy substances; It contains: sodium chloride 97.55mmol / L, potassium chloride 3.55mmol / L, magnesium sulfate 0.55mmol / L, calcium chloride 1.88mmol / L, sodium dihydrogen phosphate 0.22mmol / L, sodium citrate 0.88mmol / L , sodium bicarbonate 4.15mmol / L, HEPES and MOPS 22.50mmol / L, glucose 0.54mmol / L, sodium pyruvate 0.38mmol / L, sodium lactate 22.45mmol / L, human serum albumin 8mg / mL, trehalose 0.40mol / L L, gentamicin sulfate 14ug / mL.

[0047] A method of using sperm freezing liquid for human assisted reproduction

[0048] Sperm Freezing: Semen should be collected after 2-3 days of abstinence and liquefied at room temperature or 37°C for 15-30 minutes. Take an eq...

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Abstract

The invention discloses sperm refrigerating fluid for human assisted reproduction and a preparation method thereof. The cane sugar in the sperm refrigerating fluid is replaced trehalose. The sperm refrigerating fluid comprises 95.00-105.00 mmol/L of sodium chloride, 3.45-6.55 mmol/L of potassium chloride, 0.55-0.65 mmol/L of magnesium sulfate, 1.75-2.22 mmol/L of calcium chloride, 0.18-0.39 mmol/Lof sodium dihydrogen phosphate, 0.58-1.78 mmol/L of sodium citrate, 3.85-5.45mmol/L of sodium bicarbonate, 15.00-25.00mmol/L of HEPES and MOPS, 0.38-0.68 mmol/L of glucose, 0.28-0.42 mmol/L of sodium pyruvate, 12.48-23.68 mmol/L of sodium lactate, 5-15 mg/mL of human serum albumin, 7-14 [mu]g/mL of gentamicin sulfate, 25-35% (v/v) of glycerin, and 0.15-0.65 mol/L of trehalose. The preparation method comprises the following steps: weighing various components, dissolving the components in injection-grade water, performing filtering and sterilizing by using a 0.2 [mu]m filter membrane, performing sampling and testing, wherein the pH value is controlled to be 7.00-7.40, the osmotic pressure ranges from 1720 mOsm/Kg to 2020 mOsm/Kg, the bacterial endotoxin is less than 0.25 EU/ml, and the blastocyst formation rate is greater than or equal to 80% when 1 cell mouse embryo is cultured for 96h.

Description

technical field [0001] The invention relates to sperm freezing technology for assisted reproduction, in particular to a sperm freezing liquid for human assisted reproduction and a preparation method thereof. Background technique [0002] Sperm freezing means that men will freeze their own sperm in medical institutions with corresponding qualifications in consideration of future fertility plans to prevent future fertility risks. Sperm freezing technology is relatively mature, and sperm freezing can be used as an auxiliary for infertile patients Fertility means, some very serious oligospermia patients can freeze the precious sperm during the treatment process for intracytoplasmic sperm injection treatment, so as to prevent the development of azoospermia in the future, so that they completely lose their fertility. father's ability. [0003] Clinically, the semen ejaculated by masturbation is collected, a small amount is taken for sampling inspection, and the rest is added with...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 尹航夏旭升胡彦新于跃龙
Owner 东蕴医疗科技(上海)有限公司
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