24 results about "Sperm freezing" patented technology

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

This utility model relates to the field of cryopreservation equipment technology and discloses a portable mouse sperm cryopreservation device, including a liquid nitrogen tank. A positioning component is fixedly connected to the inner wall of the liquid nitrogen tank, and a measuring rod is slidably connected inside the positioning component. A first foam float is fixedly connected to the bottom of the measuring rod. A hanging groove is provided on the top of the liquid nitrogen tank, and a hanging rod is hung inside the hanging groove. A telescopic rod is slidably connected inside the hanging rod, and a second foam float is fixedly connected to the bottom of the telescopic rod. A placement tray is fixedly connected to the outside of the second foam float, and a pressure cap is slidably connected to the outside of the telescopic rod. This portable mouse sperm cryopreservation device allows for a direct view of the liquid nitrogen level in the liquid nitrogen tank through the cooperation of the measuring rod and the first foam float. The placement tray is connected to the hanging rod via the telescopic rod and is equipped with a pressure cap and limiting components to securely fix the cryopreservation tube.

The invention provides an ultralow-temperature preservation solution for sperms of mystus nemurus as well as a preparation method and application of the ultralow-temperature preservation solution, and belongs to the technical field of artificial breeding of mystus nemurus. The cryopreservation solution for sperms of mystus nemurus comprises a diluent and acetamide, wherein the final concentration of the acetamide is 80-150g / L; the diluent comprises the following components: sodium chloride, calcium chloride, magnesium sulfate, potassium chloride, glucose, astragalus polysaccharide and taurine. The ultra-low temperature preserving fluid effectively keeps the original pH of semen, maintains the osmotic pressure of the semen and inhibits sperm activation, the addition of acetamide reduces the freezing point of cytoplasm and prevents the formation of ice crystals, and the addition of astragalus polysaccharide and taurine improves the mitochondrial function of sperm and enhances the toughness of cell membranes. The survival rate and the functionality of the frozen sperms are effectively improved.

The invention discloses a cryopreservation method of haliotis diversicolor sperms. The method comprises the following steps: collecting the sperms, testing the initial activity of the sperms, preparing sperm freezing liquid, balancing and freezing the sperm freezing liquid, unfreezing the frozen sperms, and testing the activity after unfreezing. The collected male haliotis discus hannai sperms with the initial activity higher than 90% and a cryoprotectant are mixed according to the volume ratio of 1: 3, and sperm refrigerating fluid is obtained; transferring the sperm freezing liquid into straw, balancing, freezing, and soaking in liquid nitrogen for preservation to obtain frozen sperms; putting the straw filled with the frozen sperms into a water bath kettle, and unfreezing to obtain unfrozen sperms; finally, the straw containing the thawed sperms is cut open, the thawed sperms are transferred to a centrifugal tube, activity testing is carried out, and the activity and quality of the haliotis discus hannai sperms after cryopreservation are remarkably improved through the method.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

The invention relates to the technical field of cyclina sinensis sperm preservation, in particular to a cyclina sinensis sperm freezing preservation method. The cyclina sinensis sperm freezing preservation method comprises the following steps: (1) putting parent shellfish into a seawater culture pond, and accelerating ripening for 7-10 days by using a ripening agent; the ripening agent is prepared from a spirulina extract, a radix paeoniae alba extract, a codonopsis pilosula extract, astaxanthin and alginic acid; (2) controlling 0.05-0.15 m / s water flow to scour and stimulate the cyclina sinensis, selecting individuals with sperms discharged after the parent shellfish gametes start to be discharged, and collecting the sperms of the cyclina sinensis; and (3) mixing the cyclina sinensis sperms with a cryoprotectant, pre-cooling, and then cryopreserving for a long time. Research results show that the use of the cryoprotectant can significantly reduce the degree of DNA damage, and the protection effect may be realized through a plurality of mechanisms such as direct combination of trehalose with DNA molecules, free radical removal, maintenance of the stability of a chromatin structure and the like.

The invention discloses a method for cryopreserving sperms of oplegnathus punctatus, which comprises the following steps: on the day before the sperms of the oplegnathus punctatus, carrying out drug ripening on male oplegnathus punctatus, on the second day after ripening, collecting seminal fluid by adopting an extrusion method, and collecting the mature seminal fluid into a clean glassware for later use; mPRS diluent is prepared, the MPRS diluent and ethylene glycol are compounded to obtain the anti-freezing liquid, and the mass concentration of the ethylene glycol in the system is 15%; diluting the anti-freezing liquid and the sperm according to a volume ratio of 1: 1 to obtain a sperm mixed liquid; the sperm mixed liquid is balanced for 10 min at the temperature of 4 DEG C, then precooled for 5 min at the position 10 cm away from the liquid nitrogen level, precooled for 5 min above the liquid nitrogen level and finally put into liquid nitrogen for cryopreservation; if the cryopreserved sperm mixed liquid needs to be used, resuscitating and activating the cryopreserved sperm mixed liquid to obtain activated sperms; the survival rate of the sperms after unfreezing and resuscitation is high, and sustainable utilization and development of germplasm resources of the sphagnaceae are better facilitated.

The invention discloses an automatic sperm freezing operation device which comprises a liquid nitrogen tank, a sealing cover covers the liquid nitrogen tank, an automatic cover opening and closing mechanism is arranged between the liquid nitrogen tank and the sealing cover, a first lifting mechanism is fixed to a supporting plate on one side of the liquid nitrogen tank, and a second lifting mechanism is fixed to the first lifting mechanism. A rotating mechanism is fixed to the top of the second lifting mechanism, a swing arm is fixed to the rotating mechanism, a liquid level meter mounting probe rod and a plurality of liquid nitrogen tank lifting basket mounting probe rods are fixed to the swing arm, an ultrasonic liquid level meter is fixed to the liquid level meter mounting probe rod, and a liquid nitrogen tank lifting basket is fixed to the liquid nitrogen tank lifting basket mounting probe rods; the cover body automatic opening and closing mechanism, the first lifting mechanism, the second lifting mechanism, the rotating mechanism and the ultrasonic liquid level meter are all connected with the control cabinet. The device can be used for automatically pre-treating straws packaged with sperms, including pre-cooling and full-cooling operation in liquid nitrogen, and automatically taking out the pre-treated straws, so that the problem of frostbite in a manual pre-treatment process is prevented.

The invention provides a trace sperm freezing capturer, and belongs to the technical field of assisted reproduction. Comprising a collecting barrel, a hollow connecting pipe, an elastic protective sleeve covering the collecting barrel, an injection pump, a mounting frame, a protective cover covering the elastic protective sleeve, and a clamping mechanism arranged on a connecting section of the hollow connecting pipe. The clamping mechanism adopts a double-layer rubber sealing structure; a first rubber ring is sleeved on the connecting section, and an annular notch with a vertical wall and an inclined wall is formed in a cantilever part of the first rubber ring; the annular clamping protrusion of the second rubber ring is clamped with the notch, and the elastic arm abuts against the inner wall of the port of the protective cover. Through rigid and elastic double protection of the protection cover and the elastic protection sleeve and the locking and sealing design of the notch and the clamping protrusion, impact of external force is effectively resisted, and deformation and leakage of components are prevented. Meanwhile, through refined material selection, the risks of sample loss and cross contamination are reduced to the maximum extent, and the activity and genetic integrity of a trace sperm sample are guaranteed.

The application discloses a zebrafish commercial feeding and breeding management system and a management method thereof, an exogenous parameter management module mainly comprises an environment parameter management module and a breeding parameter control module, and can ensure normal operation of equipment in a fish house and visually record equipment parameter indexes; a breeding and feeding part comprises a breeding module, a young fish breeding module, a adult fish breeding module, a special strain construction service module and a sperm freezing management module, and is used for recording a zebrafish feeding and breeding link process in detail. The application realizes effective management of a zebrafish commercial feeding and breeding process, each module can accurately analyze a zebrafish growth state in real time, and timely response can be made, the whole feeding and breeding process has high automation, process connection is smooth, and data traceability is good. The application meets the demand of zebrafish experimental breeding, breeding, selling process management.

The utility model relates to a sperm freezing descent control device which comprises a liquid nitrogen tank used for containing liquid nitrogen, a pedestal and a controller, hollow horizontal rods are arranged on the pedestal, a winding wheel is rotatably connected between the inner side walls of the left ends of the horizontal rods, a fixed pulley is rotatably connected between the inner side walls of the right ends of the horizontal rods, and the fixed pulley is connected with the controller. A pull rope is wound on the winding wheel, and the free end of the pull rope naturally droops after bypassing the fixed pulley and is detachably connected with a hanging basket used for containing a freezing thin tube; a motor electrically connected with the controller is arranged at the left end of the horizontal rod, the output end of the motor is fixedly connected with the winding wheel, a distance sensor used for monitoring the height between the hanging basket and the liquid level of liquid nitrogen in the liquid nitrogen tank is arranged on the hanging basket, and the distance sensor is in signal connection with the controller. Different liquid nitrogen gas phase pre-freezing heights can be adjusted according to different semen qualities, so that the semen is ensured to be at a proper pre-freezing temperature, the semen freezing quality is improved, and the inactivation rate is reduced.

The application provides a large yellow croaker sperm freezing solution and a method for cryopreservation of large yellow croaker sperm, and belongs to the technical field of sperm freezing. The large yellow croaker sperm freezing solution comprises Hanks buffer solution, ergothioneine, glycerol and trehalose. The application adopts a step-by-step cooling method, first cools the mixed solution to-5 to-3 DEG C, and then cools to-197 to-195 DEG C after cold equilibrium, and then is transferred into a liquid nitrogen tank for freezing storage. Before use, thawing and recovery are carried out in a 36 to 38 DEG C water bath. The large yellow croaker sperm freezing solution can effectively protect sperm, and can significantly improve the survival rate of recovered sperm in the subsequent sperm recovery process, thereby providing a new idea for the freezing of large yellow croaker sperm.

The invention belongs to the technical field of alpaca artificial insemination, and particularly relates to a cryopreservation agent for alpaca semen, a preparation method and application. The invention provides a cryopreservation agent for alpaca semen. The cryopreservation agent comprises trihydroxymethyl aminomethane, citric acid, fructose, penicillin sodium, streptomycin sulfate, yolk, glycerin and glucose. Wherein the yolk and the glycerol have a synergistic effect, so that the freezing resuscitation rate of sperms is increased; and citric acid and trihydroxymethyl aminomethane are used as buffering agents to stabilize the pH value of the semen diluent, maintain the activity and function of the semen diluent and reduce the damage of free radicals to sperms. The penicillin sodium and the streptomycin sulfate are used for sterilization. The fructose, the yolk, the glycerol and the glucose have an anti-oxidation effect, the cell structure of sperms is stabilized, the activity of the sperms is maintained, and the citric acid and the saccharides are matched to regulate osmotic pressure so as to protect the sperms from being damaged by freezing. The preparation method of the cryopreservation agent is simple and easy, and the sperm motility of the alpaca semen after cryopreservation can be improved.

The semen freezing equipment comprises a container assembly, a freezing pipe, a freezing pipe placement support, a driving mechanism, a refrigeration assembly and a controller, the container assembly is internally provided with a containing space capable of storing liquid nitrogen and a channel connected with the containing space, and the refrigeration assembly is used for refrigerating the interior of the channel; the controller can control the driving mechanism to drive the bracket to enter the channel and precool the semen in the freezing pipe on the bracket, and can also control the driving mechanism to drive the bracket of which the semen in the freezing pipe on the bracket is precooled to enter the liquid nitrogen in the accommodating space through the channel to freeze the semen. Diversified cooling rates and cooling time can be set, and different sperm freezing requirements can be met.

The application discloses a SNP site for identifying the sperm freezing tolerance of Chinese Holstein bulls. The SNP site is the nucleotide at the 161th position from the 5' end of SEQ ID NO:1 in the genome of the bull. By detecting the genotype of the SNP site in the genome of the bull to be tested, the sperm freezing tolerance of the Chinese Holstein bull can be screened or assisted to be screened. The method is not only simple, rapid, sensitive, but also reliable, stable and accurate. The application is suitable for large population scale detection and has important application value.

The utility model discloses a simple and convenient human sperm freezing recovery device, include: box, place subassembly, place subassembly includes the support plate of sliding installation in the inner wall of box, sets up the fixed plate of support plate top outer wall, equidistance opens the support groove of setting in support plate top outer wall, equidistance opens the fixed hole of setting on the fixed plate, sets up the protective sleeve of fixed hole and support groove inner wall, wipe subassembly, wipe subassembly includes the connecting block of welding in the inner wall top of both sides of box, and the wiping board of connecting block joint. The utility model discloses the mutual cooperation of setting up's box, place subassembly and wipe subassembly, when the recovery of the cryopreserved tube that has stored semen, need not the operator to contact the cryopreserved tube, prevent the operator from frequently contacting the cryopreserved tube during the recovery process and cause the recovery semen to move constantly to thereby influence the semen recovery, and can recover several semen at a time, and the semen recovery time and recovery environment can keep consistent.

The invention relates to the technical field of animal genetic breeding, and particularly discloses a molecular marker group for identifying freezing tolerance of pig sperms and application of the molecular marker group, the molecular marker group comprises three specific SNP marker groups: a group A is a Duroc pig specific marker and comprises 10 SNPs such as rs322459109; the group B is a large white pig specific marker and comprises 33 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like; and the group C is a cross-variety universal marker and comprises 12 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like. The markers are located in the gene regions of PARN, SOX9, CCDC181, DHH, NCKAP5L, MAP2K6, MPV 17 and DRC1. The invention further provides a kit containing the detection primer and / or probe, a boar sperm freezing tolerance evaluation method, a boar breeding method, application of candidate genes, a computer readable storage medium and electronic equipment, early-stage, rapid and accurate evaluation of boar sperm freezing tolerance can be achieved, the breeding efficiency is remarkably improved, the breeding cost is reduced, and the method is suitable for large-scale popularization and application. And an important technical support is provided for industrial application of a pig frozen semen technology.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, an in vitro fertilization medium, and methods for treating and / or preventing infection, replication, and transmission of viruses, such as sexually transmitted viruses.

Anchovy sperm freezing preservation solution, and a preparation method and application method thereof, belong to the technical field of aquatic germplasm resource protection and breeding, and particularly relate to a method for collecting, preserving and recovering sperm of anchovy fish. The present application is to solve the problems of low efficiency, short preservation time and low sperm activity of the existing anchovy sperm collection, and the anchovy sperm freezing preservation solution is composed of methanol, lecithin, resveratrol and glucose, and is diluted with double distilled water to 1L. The application method is as follows: 1. sperm collection and quality evaluation; 2. preparation of anchovy sperm freezing preservation solution; 3. mixing the sperm with the pre-cooled 4 DEG C anchovy sperm freezing preservation solution, dispensing, and adopting a four-step method for programmed cooling; 4. oocyte collection; 5. sperm warming and activity detection; 6. artificial insemination and hatching. The sperm preservation time is prolonged from several hours to more than several years, and through rapid thawing, the sperm activity can reach more than 40%, and the fertilization rate can reach more than 57%.

The invention provides an application of pyridoxamine in sperm optimization and / or freezing recovery operation of asthenospermia, pyridoxamine is added in sperm operation liquid or sperm freezing liquid, and the following shortages of asthenospermia patients are effectively improved: (1) the ratio of sperm forward motility sperms; (2) determining the ratio of forward motility sperms after the semen is frozen and resuscitated; (3) determining the sperm DNA fragment rate after the sperm is frozen and resuscitated; (4) determining the content of sperm acrosomal enzyme after the sperm is frozen and resuscitated; in conclusion, the pyridoxamine can be added into sperm operating fluid or sperm refrigerating fluid as a natural sperm protective agent, the sperm optimization treatment and freezing resuscitation effects of the asthenospermia patient are obviously improved, and the pyridoxamine is suitable for being applied to assisted reproductive therapy and fertility preservation of the asthenospermia patient.

The invention discloses a sperm unfreezing device and an unfreezing method for frozen sperms.The sperm unfreezing device comprises an unfreezing box, a water bath heating assembly and an air heating assembly are arranged in the unfreezing box, the water bath heating assembly comprises a water bath heating channel, and the air heating assembly comprises an air heating channel; the air heating channel is communicated with the water bath heating channel, a plurality of fixing assemblies are arranged in the unfreezing box, and the fixing assemblies are connected through a driving rope. A plurality of semen freezing tubes can be continuously treated, the treatment efficiency is greatly improved, rapid unfreezing of the semen freezing tubes is guaranteed through water bath heating, the semen freezing tubes are heated again through air heating, it is guaranteed that the temperature of the semen freezing tubes reaches the standard, meanwhile, the surfaces of the semen freezing tubes are dried, and the semen freezing tubes can be conveniently loaded into a semen deposition gun for semen deposition in the next step; when the fixing assembly moves to the position of the discharging port, automatic releasing of the sperm freezing pipe is achieved, the sperm freezing pipe can be taken out conveniently, a new sperm freezing pipe can be placed in conveniently, manual operation is reduced, and labor intensity is lowered.

The invention discloses a rewarming and optimizing device for testicular sperms of NOA patients after freezing, which comprises a sealed operation box, a temperature and humidity sensor is fixed on a rear panel of the sealed operation box, a cold air inlet is arranged below a left side plate of the sealed operation box, and a hot air outlet is arranged above the left side plate of the sealed operation box. A constant-temperature water bath box, a water bath cup, a sperm rewarming test tube, a first peristaltic pump, a sperm optimization bionic channel, a second peristaltic pump and a sperm screening and collecting tube are fixed in the sealed operation box, an air heater, a storage battery and a PLC industrial personal computer are fixed in a functional frame of the sealed operation box, and the air heater and the storage battery are connected with the PLC industrial personal computer. The device integrates sperm rewarming and optimizing functions and is carried out in a closed space, the pollution problem caused in the sperm transfer process can be reduced, meanwhile, the device can provide a screening channel closer to the uterus environment, and therefore the high-quality screening work of sperms is completed.

The invention discloses application of orientin in improvement of frozen sperm quality and in-vitro cryopreservation of sperms. The invention further discloses a sperm cryopreservation solution, and the sperm cryopreservation solution contains orientin. Orientin is found to have a good protective effect in the sperm freezing process; through multiple mechanisms of reducing oxidative stress, improving mitochondrial functions, improving sperm ultrastructures and the like, the activity and quality of sperms are improved.