35 results about "Sperm freezing" patented technology

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, an in vitro fertilization medium, and methods for treating and / or preventing infection, replication, and transmission of viruses, such as sexually transmitted viruses.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

This utility model relates to the field of cryopreservation equipment technology and discloses a portable mouse sperm cryopreservation device, including a liquid nitrogen tank. A positioning component is fixedly connected to the inner wall of the liquid nitrogen tank, and a measuring rod is slidably connected inside the positioning component. A first foam float is fixedly connected to the bottom of the measuring rod. A hanging groove is provided on the top of the liquid nitrogen tank, and a hanging rod is hung inside the hanging groove. A telescopic rod is slidably connected inside the hanging rod, and a second foam float is fixedly connected to the bottom of the telescopic rod. A placement tray is fixedly connected to the outside of the second foam float, and a pressure cap is slidably connected to the outside of the telescopic rod. This portable mouse sperm cryopreservation device allows for a direct view of the liquid nitrogen level in the liquid nitrogen tank through the cooperation of the measuring rod and the first foam float. The placement tray is connected to the hanging rod via the telescopic rod and is equipped with a pressure cap and limiting components to securely fix the cryopreservation tube.

The invention provides a feed for improving sperm quality after sperm freezing as well as a preparation method and application thereof, and belongs to the technical field of animal feeds. The feed for improving the sperm quality of breeding male rabbits and improving the conception rate of female rabbits comprises the following components in parts by weight: 10-20 parts of corn, 50-60 parts of soybean meal, 12-20 parts of fish meal, 5-10 parts of premix and 0.1-0.3 part of vitamin E. The feed improves the sperm motility of fresh sperms, the sperm motility after freezing, the acrosome integrity rate, the plasma membrane integrity rate and the mitochondrial membrane potential of the sperms after the fresh sperms and the frozen sperms.

The invention provides an ultralow-temperature preservation solution for sperms of mystus nemurus as well as a preparation method and application of the ultralow-temperature preservation solution, and belongs to the technical field of artificial breeding of mystus nemurus. The cryopreservation solution for sperms of mystus nemurus comprises a diluent and acetamide, wherein the final concentration of the acetamide is 80-150g / L; the diluent comprises the following components: sodium chloride, calcium chloride, magnesium sulfate, potassium chloride, glucose, astragalus polysaccharide and taurine. The ultra-low temperature preserving fluid effectively keeps the original pH of semen, maintains the osmotic pressure of the semen and inhibits sperm activation, the addition of acetamide reduces the freezing point of cytoplasm and prevents the formation of ice crystals, and the addition of astragalus polysaccharide and taurine improves the mitochondrial function of sperm and enhances the toughness of cell membranes. The survival rate and the functionality of the frozen sperms are effectively improved.

The invention discloses a cryopreservation method of haliotis diversicolor sperms. The method comprises the following steps: collecting the sperms, testing the initial activity of the sperms, preparing sperm freezing liquid, balancing and freezing the sperm freezing liquid, unfreezing the frozen sperms, and testing the activity after unfreezing. The collected male haliotis discus hannai sperms with the initial activity higher than 90% and a cryoprotectant are mixed according to the volume ratio of 1: 3, and sperm refrigerating fluid is obtained; transferring the sperm freezing liquid into straw, balancing, freezing, and soaking in liquid nitrogen for preservation to obtain frozen sperms; putting the straw filled with the frozen sperms into a water bath kettle, and unfreezing to obtain unfrozen sperms; finally, the straw containing the thawed sperms is cut open, the thawed sperms are transferred to a centrifugal tube, activity testing is carried out, and the activity and quality of the haliotis discus hannai sperms after cryopreservation are remarkably improved through the method.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

The invention relates to the technical field of cyclina sinensis sperm preservation, in particular to a cyclina sinensis sperm freezing preservation method. The cyclina sinensis sperm freezing preservation method comprises the following steps: (1) putting parent shellfish into a seawater culture pond, and accelerating ripening for 7-10 days by using a ripening agent; the ripening agent is prepared from a spirulina extract, a radix paeoniae alba extract, a codonopsis pilosula extract, astaxanthin and alginic acid; (2) controlling 0.05-0.15 m / s water flow to scour and stimulate the cyclina sinensis, selecting individuals with sperms discharged after the parent shellfish gametes start to be discharged, and collecting the sperms of the cyclina sinensis; and (3) mixing the cyclina sinensis sperms with a cryoprotectant, pre-cooling, and then cryopreserving for a long time. Research results show that the use of the cryoprotectant can significantly reduce the degree of DNA damage, and the protection effect may be realized through a plurality of mechanisms such as direct combination of trehalose with DNA molecules, free radical removal, maintenance of the stability of a chromatin structure and the like.

The invention discloses a method for cryopreserving sperms of oplegnathus punctatus, which comprises the following steps: on the day before the sperms of the oplegnathus punctatus, carrying out drug ripening on male oplegnathus punctatus, on the second day after ripening, collecting seminal fluid by adopting an extrusion method, and collecting the mature seminal fluid into a clean glassware for later use; mPRS diluent is prepared, the MPRS diluent and ethylene glycol are compounded to obtain the anti-freezing liquid, and the mass concentration of the ethylene glycol in the system is 15%; diluting the anti-freezing liquid and the sperm according to a volume ratio of 1: 1 to obtain a sperm mixed liquid; the sperm mixed liquid is balanced for 10 min at the temperature of 4 DEG C, then precooled for 5 min at the position 10 cm away from the liquid nitrogen level, precooled for 5 min above the liquid nitrogen level and finally put into liquid nitrogen for cryopreservation; if the cryopreserved sperm mixed liquid needs to be used, resuscitating and activating the cryopreserved sperm mixed liquid to obtain activated sperms; the survival rate of the sperms after unfreezing and resuscitation is high, and sustainable utilization and development of germplasm resources of the sphagnaceae are better facilitated.

The utility model relates to a trace sperm freezing carrying rod in the technical field of sperm storage, which comprises a carrying rod body and a carrying rod sleeve, the carrying rod body comprises a handheld part and a connecting part, and a freezing part is arranged at the tail end of the connecting part. And the storage amount of the sperm refrigerating fluid in the refrigerating part is 20 microliters. According to the sperm freezing carrying rod, the freezing part is designed into the semicircular tube shape and is provided with the strip-shaped long groove with the semicircular cross section, sperm freezing liquid can be stored in the whole long groove, the storage amount of the sperm freezing liquid of the freezing part reaches 20 microliters, and compared with an existing sperm freezing carrying rod, the sperm freezing carrying rod has the advantage that the storage amount of the sperm freezing liquid is increased.

The present invention provides a SNP genetic marker downstream of the SUN5 gene associated with pig sperm freeze tolerance, belonging to the technical field of pig genetic marker detection. The SNP genetic marker is named SUN5DOWN239 and is located at the 36507861bp nucleotide position on pig chromosome 17 of the International Porcine Genome Version 11.1 reference sequence. The base at this position is G or C. The SUN5DOWN239 is located 239bp downstream of the SUN5 gene, corresponding to the 76bp position in the nucleotide sequence shown in SEQ ID NO:1. This SNP genetic marker can help improve the quality of frozen semen of boars, provide a new marker resource for auxiliary selection of boars, and can be used in boar genetic breeding. The present invention also provides an application of a SNP genetic marker downstream of the SUN5 gene associated with pig sperm freeze tolerance in pig genetic breeding.

The invention belongs to the technical field of semen cryopreservation, and particularly relates to a sheep semen cryopreservation solution and a semen preservation method. The sheep semen freezing medium comprises the following components: Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soya bean lecithin, nobiletin, glycerol and water. The nobiletin with a specific content is used as an effective antioxidant component of the sheep sperm cryopreservation agent, so that the nobiletin plays an antioxidant role when the sperms are frozen, and the sperms are more stable in the cryopreservation process. After nobiletin is added, the activity and movement performance of frozen and unfrozen sheep sperms can be remarkably improved. The oxidative stress injury of the sperms in the freezing process is effectively reduced, and the oxidation resistance of the sperms is improved.

The invention relates to a single sperm freezing carrier, and relates to the technical field of medical treatment and public health. Comprising a cryopreservation tube, a freezing slide glass and a connecting handle, the freezing slide glass and the connecting handle are integrally formed in a pouring mode, and a plurality of freezing slide glass are stored in one freezing tube. The device has the characteristics of miniaturization, simplification and integration, and increases the number of frozen patients and the number of times of use on the premise of not increasing the freezing charge of the patients.

The invention discloses an automatic sperm freezing operation device which comprises a liquid nitrogen tank, a sealing cover covers the liquid nitrogen tank, an automatic cover opening and closing mechanism is arranged between the liquid nitrogen tank and the sealing cover, a first lifting mechanism is fixed to a supporting plate on one side of the liquid nitrogen tank, and a second lifting mechanism is fixed to the first lifting mechanism. A rotating mechanism is fixed to the top of the second lifting mechanism, a swing arm is fixed to the rotating mechanism, a liquid level meter mounting probe rod and a plurality of liquid nitrogen tank lifting basket mounting probe rods are fixed to the swing arm, an ultrasonic liquid level meter is fixed to the liquid level meter mounting probe rod, and a liquid nitrogen tank lifting basket is fixed to the liquid nitrogen tank lifting basket mounting probe rods; the cover body automatic opening and closing mechanism, the first lifting mechanism, the second lifting mechanism, the rotating mechanism and the ultrasonic liquid level meter are all connected with the control cabinet. The device can be used for automatically pre-treating straws packaged with sperms, including pre-cooling and full-cooling operation in liquid nitrogen, and automatically taking out the pre-treated straws, so that the problem of frostbite in a manual pre-treatment process is prevented.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, an in vitro fertilization medium, and methods for treating and / or preventing infection, replication, and transmission of viruses, such as sexually transmitted viruses.

The invention provides a trace sperm freezing capturer, and belongs to the technical field of assisted reproduction. Comprising a collecting barrel, a hollow connecting pipe, an elastic protective sleeve covering the collecting barrel, an injection pump, a mounting frame, a protective cover covering the elastic protective sleeve, and a clamping mechanism arranged on a connecting section of the hollow connecting pipe. The clamping mechanism adopts a double-layer rubber sealing structure; a first rubber ring is sleeved on the connecting section, and an annular notch with a vertical wall and an inclined wall is formed in a cantilever part of the first rubber ring; the annular clamping protrusion of the second rubber ring is clamped with the notch, and the elastic arm abuts against the inner wall of the port of the protective cover. Through rigid and elastic double protection of the protection cover and the elastic protection sleeve and the locking and sealing design of the notch and the clamping protrusion, impact of external force is effectively resisted, and deformation and leakage of components are prevented. Meanwhile, through refined material selection, the risks of sample loss and cross contamination are reduced to the maximum extent, and the activity and genetic integrity of a trace sperm sample are guaranteed.

The application discloses a zebrafish commercial feeding and breeding management system and a management method thereof, an exogenous parameter management module mainly comprises an environment parameter management module and a breeding parameter control module, and can ensure normal operation of equipment in a fish house and visually record equipment parameter indexes; a breeding and feeding part comprises a breeding module, a young fish breeding module, a adult fish breeding module, a special strain construction service module and a sperm freezing management module, and is used for recording a zebrafish feeding and breeding link process in detail. The application realizes effective management of a zebrafish commercial feeding and breeding process, each module can accurately analyze a zebrafish growth state in real time, and timely response can be made, the whole feeding and breeding process has high automation, process connection is smooth, and data traceability is good. The application meets the demand of zebrafish experimental breeding, breeding, selling process management.

The utility model relates to a sperm freezing descent control device which comprises a liquid nitrogen tank used for containing liquid nitrogen, a pedestal and a controller, hollow horizontal rods are arranged on the pedestal, a winding wheel is rotatably connected between the inner side walls of the left ends of the horizontal rods, a fixed pulley is rotatably connected between the inner side walls of the right ends of the horizontal rods, and the fixed pulley is connected with the controller. A pull rope is wound on the winding wheel, and the free end of the pull rope naturally droops after bypassing the fixed pulley and is detachably connected with a hanging basket used for containing a freezing thin tube; a motor electrically connected with the controller is arranged at the left end of the horizontal rod, the output end of the motor is fixedly connected with the winding wheel, a distance sensor used for monitoring the height between the hanging basket and the liquid level of liquid nitrogen in the liquid nitrogen tank is arranged on the hanging basket, and the distance sensor is in signal connection with the controller. Different liquid nitrogen gas phase pre-freezing heights can be adjusted according to different semen qualities, so that the semen is ensured to be at a proper pre-freezing temperature, the semen freezing quality is improved, and the inactivation rate is reduced.

The application provides a large yellow croaker sperm freezing solution and a method for cryopreservation of large yellow croaker sperm, and belongs to the technical field of sperm freezing. The large yellow croaker sperm freezing solution comprises Hanks buffer solution, ergothioneine, glycerol and trehalose. The application adopts a step-by-step cooling method, first cools the mixed solution to-5 to-3 DEG C, and then cools to-197 to-195 DEG C after cold equilibrium, and then is transferred into a liquid nitrogen tank for freezing storage. Before use, thawing and recovery are carried out in a 36 to 38 DEG C water bath. The large yellow croaker sperm freezing solution can effectively protect sperm, and can significantly improve the survival rate of recovered sperm in the subsequent sperm recovery process, thereby providing a new idea for the freezing of large yellow croaker sperm.

The invention belongs to the technical field of alpaca artificial insemination, and particularly relates to a cryopreservation agent for alpaca semen, a preparation method and application. The invention provides a cryopreservation agent for alpaca semen. The cryopreservation agent comprises trihydroxymethyl aminomethane, citric acid, fructose, penicillin sodium, streptomycin sulfate, yolk, glycerin and glucose. Wherein the yolk and the glycerol have a synergistic effect, so that the freezing resuscitation rate of sperms is increased; and citric acid and trihydroxymethyl aminomethane are used as buffering agents to stabilize the pH value of the semen diluent, maintain the activity and function of the semen diluent and reduce the damage of free radicals to sperms. The penicillin sodium and the streptomycin sulfate are used for sterilization. The fructose, the yolk, the glycerol and the glucose have an anti-oxidation effect, the cell structure of sperms is stabilized, the activity of the sperms is maintained, and the citric acid and the saccharides are matched to regulate osmotic pressure so as to protect the sperms from being damaged by freezing. The preparation method of the cryopreservation agent is simple and easy, and the sperm motility of the alpaca semen after cryopreservation can be improved.

The semen freezing equipment comprises a container assembly, a freezing pipe, a freezing pipe placement support, a driving mechanism, a refrigeration assembly and a controller, the container assembly is internally provided with a containing space capable of storing liquid nitrogen and a channel connected with the containing space, and the refrigeration assembly is used for refrigerating the interior of the channel; the controller can control the driving mechanism to drive the bracket to enter the channel and precool the semen in the freezing pipe on the bracket, and can also control the driving mechanism to drive the bracket of which the semen in the freezing pipe on the bracket is precooled to enter the liquid nitrogen in the accommodating space through the channel to freeze the semen. Diversified cooling rates and cooling time can be set, and different sperm freezing requirements can be met.

The utility model relates to the technical field of cattle breeding sperm freezing, and discloses a beef cattle mating sperm freezing device which comprises a freezing sealing box, the front face of the freezing sealing box is connected with a sealing plate through a hinge, one side of the sealing plate is provided with a temperature displayer, the temperature displayer is a DT-670 low-temperature temperature sensor, and the sealing plate is provided with a temperature sensor. And a liquid nitrogen conveying mechanism is arranged at the top end of the freezing sealing box. The beef cattle sperm storage tube deblocking device has the following advantages and effects that a beef cattle sperm storage tube needing to be deblocked is placed in a circular groove, a hydraulic oil cylinder stretches out and draws back to drive a rack plate to move, the rack plate drives a first gear to rotate, then the first gear drives a second gear and a circular rod to rotate, and the beef cattle sperm storage tube is deblocked; the rotating disc and the deblocking disc are driven by the round rod to rotate, so that the beef cattle sperm preservation tube placed in the deblocking disc is uniformly rotated in warm water to be quickly deblocked, and the deblocking efficiency of the device is improved.

The application discloses a SNP site for identifying the sperm freezing tolerance of Chinese Holstein bulls. The SNP site is the nucleotide at the 161th position from the 5' end of SEQ ID NO:1 in the genome of the bull. By detecting the genotype of the SNP site in the genome of the bull to be tested, the sperm freezing tolerance of the Chinese Holstein bull can be screened or assisted to be screened. The method is not only simple, rapid, sensitive, but also reliable, stable and accurate. The application is suitable for large population scale detection and has important application value.

The utility model discloses a simple and convenient human sperm freezing recovery device, include: box, place subassembly, place subassembly includes the support plate of sliding installation in the inner wall of box, sets up the fixed plate of support plate top outer wall, equidistance opens the support groove of setting in support plate top outer wall, equidistance opens the fixed hole of setting on the fixed plate, sets up the protective sleeve of fixed hole and support groove inner wall, wipe subassembly, wipe subassembly includes the connecting block of welding in the inner wall top of both sides of box, and the wiping board of connecting block joint. The utility model discloses the mutual cooperation of setting up's box, place subassembly and wipe subassembly, when the recovery of the cryopreserved tube that has stored semen, need not the operator to contact the cryopreserved tube, prevent the operator from frequently contacting the cryopreserved tube during the recovery process and cause the recovery semen to move constantly to thereby influence the semen recovery, and can recover several semen at a time, and the semen recovery time and recovery environment can keep consistent.

The utility model relates to a sperm freezing carrier and a sperm preservation device, the sperm freezing carrier comprises a slide glass body, the surface of the slide glass body is divided into a hydrophobic area and a screening area, the hydrophobic area surrounds the screening area, a hydrophobic layer is arranged on the hydrophobic area, and a hyaluronic acid layer is arranged in the screening area. By means of the hydrophobic effect of the hydrophobic layer on the hydrophobic area and the tension of the cryoprotectant, the cryoprotectant cannot leak out from the hydrophobic area, the cryoprotectant is effectively prevented from splashing out in the operation process, and the probability of sperm loss is reduced. The mature sperms can be adhered to the hyaluronic acid layer of the screening area, so that the recovery rate of the frozen sperms is improved, the purpose of screening high-quality sperms can be achieved, and meanwhile, the time for searching the sperms in the cryoprotectant liquid drops subsequently can be shortened.

The invention relates to the technical field of animal genetic breeding, and particularly discloses a molecular marker group for identifying freezing tolerance of pig sperms and application of the molecular marker group, the molecular marker group comprises three specific SNP marker groups: a group A is a Duroc pig specific marker and comprises 10 SNPs such as rs322459109; the group B is a large white pig specific marker and comprises 33 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like; and the group C is a cross-variety universal marker and comprises 12 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like. The markers are located in the gene regions of PARN, SOX9, CCDC181, DHH, NCKAP5L, MAP2K6, MPV 17 and DRC1. The invention further provides a kit containing the detection primer and / or probe, a boar sperm freezing tolerance evaluation method, a boar breeding method, application of candidate genes, a computer readable storage medium and electronic equipment, early-stage, rapid and accurate evaluation of boar sperm freezing tolerance can be achieved, the breeding efficiency is remarkably improved, the breeding cost is reduced, and the method is suitable for large-scale popularization and application. And an important technical support is provided for industrial application of a pig frozen semen technology.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, an in vitro fertilization medium, and methods for treating and / or preventing infection, replication, and transmission of viruses, such as sexually transmitted viruses.

The invention discloses an ultramicro sperm freezing piece carrier with a sperm wandering limiting function, and particularly relates to the technical field of sperm freezing, the ultramicro sperm freezing piece carrier comprises a freezing pipe and a sealing cover, three freezing piece carriers which can be detachably spliced are placed in the freezing pipe, and a slide glass is embedded in the middle of the upper end of each freezing piece carrier; five placing pools are evenly arranged at the upper end of the slide glass, slopes inclining upwards are formed on the upper portions of the rear walls of the placing pools, and the tails of the slopes are smoothly connected with the slide glass and the upper surface of the freezing piece carrier. According to the ultra-micro sperm freezing sheet carrier with the sperm migration limiting function, five placement pools are formed in a traditional high borosilicate glass plain sheet, sperms are placed in the placement pools, the sperm activity range is limited through the placement pools, the loss risk is reduced, meanwhile, corresponding numbers are arranged beside the placement pools, and the sperms are prevented from being migrated through the placement pools. The sperm positioning efficiency can be improved, and the ovum in-vitro exposure time can be shortened.

Anchovy sperm freezing preservation solution, and a preparation method and application method thereof, belong to the technical field of aquatic germplasm resource protection and breeding, and particularly relate to a method for collecting, preserving and recovering sperm of anchovy fish. The present application is to solve the problems of low efficiency, short preservation time and low sperm activity of the existing anchovy sperm collection, and the anchovy sperm freezing preservation solution is composed of methanol, lecithin, resveratrol and glucose, and is diluted with double distilled water to 1L. The application method is as follows: 1. sperm collection and quality evaluation; 2. preparation of anchovy sperm freezing preservation solution; 3. mixing the sperm with the pre-cooled 4 DEG C anchovy sperm freezing preservation solution, dispensing, and adopting a four-step method for programmed cooling; 4. oocyte collection; 5. sperm warming and activity detection; 6. artificial insemination and hatching. The sperm preservation time is prolonged from several hours to more than several years, and through rapid thawing, the sperm activity can reach more than 40%, and the fertilization rate can reach more than 57%.