The invention discloses a trace
sperm cryopreservation carrier and corresponding freezing and thawing methods thereof. The trace
sperm cryopreservation carrier is in a rectangular, square or circular horizontal or groove shape and is made of a transparent plastic or transparent glass, and the size of the trace
sperm cryopreservation carrier can bear 1-3 0.1-10.0 mu L freezing liquid micro drops. The freezing and thawing method comprises the following steps: after preparing the freezing liquid micro drops on the trace
sperm cryopreservation carrier, inserting the trace
sperm cryopreservation carrier into a sperm culture dish covered with oil, grabbing sperms by a
microinjection needle, putting into the freezing liquid micro drops, taking out the cryopreservation carrier, soaking up
mineral oil by virtue of an absorbent paper, rapidly freezing, directly inserting the cryopreservation carrier into an ICSI vessel when thawing, and thawing the sperms by utilizing the
mineral oil with the temperature of 37 DEG C. According to the trace
sperm cryopreservation carrier and the corresponding freezing and thawing method thereof disclosed by the invention, as the cryopreservation carrier is inserted into the sperm culture dish covered with oil, and the sperms are grabbed and frozen, a special
moisture retention device is not needed, and
evaporation of the freezing liquid micro drops is not needed to be worried about; the operation is very simple, the flexibility and reliability of assisted reproductive treatment for a patient with
azoospermia are greatly improved, the sperm
recovery rate can reach 100 percent, and the sperm viability is about 60 percent.