6 results about "Sperm freezing" patented technology

This utility model relates to the field of cryopreservation equipment technology and discloses a portable mouse sperm cryopreservation device, including a liquid nitrogen tank. A positioning component is fixedly connected to the inner wall of the liquid nitrogen tank, and a measuring rod is slidably connected inside the positioning component. A first foam float is fixedly connected to the bottom of the measuring rod. A hanging groove is provided on the top of the liquid nitrogen tank, and a hanging rod is hung inside the hanging groove. A telescopic rod is slidably connected inside the hanging rod, and a second foam float is fixedly connected to the bottom of the telescopic rod. A placement tray is fixedly connected to the outside of the second foam float, and a pressure cap is slidably connected to the outside of the telescopic rod. This portable mouse sperm cryopreservation device allows for a direct view of the liquid nitrogen level in the liquid nitrogen tank through the cooperation of the measuring rod and the first foam float. The placement tray is connected to the hanging rod via the telescopic rod and is equipped with a pressure cap and limiting components to securely fix the cryopreservation tube.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

The utility model relates to a sperm freezing descent control device which comprises a liquid nitrogen tank used for containing liquid nitrogen, a pedestal and a controller, hollow horizontal rods are arranged on the pedestal, a winding wheel is rotatably connected between the inner side walls of the left ends of the horizontal rods, a fixed pulley is rotatably connected between the inner side walls of the right ends of the horizontal rods, and the fixed pulley is connected with the controller. A pull rope is wound on the winding wheel, and the free end of the pull rope naturally droops after bypassing the fixed pulley and is detachably connected with a hanging basket used for containing a freezing thin tube; a motor electrically connected with the controller is arranged at the left end of the horizontal rod, the output end of the motor is fixedly connected with the winding wheel, a distance sensor used for monitoring the height between the hanging basket and the liquid level of liquid nitrogen in the liquid nitrogen tank is arranged on the hanging basket, and the distance sensor is in signal connection with the controller. Different liquid nitrogen gas phase pre-freezing heights can be adjusted according to different semen qualities, so that the semen is ensured to be at a proper pre-freezing temperature, the semen freezing quality is improved, and the inactivation rate is reduced.

The application discloses a SNP site for identifying the sperm freezing tolerance of Chinese Holstein bulls. The SNP site is the nucleotide at the 161th position from the 5' end of SEQ ID NO:1 in the genome of the bull. By detecting the genotype of the SNP site in the genome of the bull to be tested, the sperm freezing tolerance of the Chinese Holstein bull can be screened or assisted to be screened. The method is not only simple, rapid, sensitive, but also reliable, stable and accurate. The application is suitable for large population scale detection and has important application value.

The invention relates to the technical field of animal genetic breeding, and particularly discloses a molecular marker group for identifying freezing tolerance of pig sperms and application of the molecular marker group, the molecular marker group comprises three specific SNP marker groups: a group A is a Duroc pig specific marker and comprises 10 SNPs such as rs322459109; the group B is a large white pig specific marker and comprises 33 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like; and the group C is a cross-variety universal marker and comprises 12 SNPs (Single Nucleotide Polymorphism) such as yzrs336886585 and the like. The markers are located in the gene regions of PARN, SOX9, CCDC181, DHH, NCKAP5L, MAP2K6, MPV 17 and DRC1. The invention further provides a kit containing the detection primer and / or probe, a boar sperm freezing tolerance evaluation method, a boar breeding method, application of candidate genes, a computer readable storage medium and electronic equipment, early-stage, rapid and accurate evaluation of boar sperm freezing tolerance can be achieved, the breeding efficiency is remarkably improved, the breeding cost is reduced, and the method is suitable for large-scale popularization and application. And an important technical support is provided for industrial application of a pig frozen semen technology.

Anchovy sperm freezing preservation solution, and a preparation method and application method thereof, belong to the technical field of aquatic germplasm resource protection and breeding, and particularly relate to a method for collecting, preserving and recovering sperm of anchovy fish. The present application is to solve the problems of low efficiency, short preservation time and low sperm activity of the existing anchovy sperm collection, and the anchovy sperm freezing preservation solution is composed of methanol, lecithin, resveratrol and glucose, and is diluted with double distilled water to 1L. The application method is as follows: 1. sperm collection and quality evaluation; 2. preparation of anchovy sperm freezing preservation solution; 3. mixing the sperm with the pre-cooled 4 DEG C anchovy sperm freezing preservation solution, dispensing, and adopting a four-step method for programmed cooling; 4. oocyte collection; 5. sperm warming and activity detection; 6. artificial insemination and hatching. The sperm preservation time is prolonged from several hours to more than several years, and through rapid thawing, the sperm activity can reach more than 40%, and the fertilization rate can reach more than 57%.