Micro sperm freezing capturer

Abstract

The invention provides a trace sperm freezing capturer, and belongs to the technical field of assisted reproduction. Comprising a collecting barrel, a hollow connecting pipe, an elastic protective sleeve covering the collecting barrel, an injection pump, a mounting frame, a protective cover covering the elastic protective sleeve, and a clamping mechanism arranged on a connecting section of the hollow connecting pipe. The clamping mechanism adopts a double-layer rubber sealing structure; a first rubber ring is sleeved on the connecting section, and an annular notch with a vertical wall and an inclined wall is formed in a cantilever part of the first rubber ring; the annular clamping protrusion of the second rubber ring is clamped with the notch, and the elastic arm abuts against the inner wall of the port of the protective cover. Through rigid and elastic double protection of the protection cover and the elastic protection sleeve and the locking and sealing design of the notch and the clamping protrusion, impact of external force is effectively resisted, and deformation and leakage of components are prevented. Meanwhile, through refined material selection, the risks of sample loss and cross contamination are reduced to the maximum extent, and the activity and genetic integrity of a trace sperm sample are guaranteed.

Description

Technical Field

[0001] This invention relates to the field of medical assisted reproductive technology, specifically to a micro-sperm cryopreservation device. Background Technology

[0002] In the cutting-edge and significant medical field of assisted reproductive technology, the cryopreservation of micro-sperm samples plays an irreplaceable and crucial role. It provides essential and extremely valuable sperm resources for subsequent complex and critical reproductive procedures such as in vitro fertilization and intracytoplasmic sperm injection (ICSI). In the arduous journey of overcoming male infertility and helping numerous families yearning for children realize their dreams of parenthood, micro-sperm cryopreservation technology plays a vital role and is an indispensable "powerful tool" in our clinical work.

[0003] In the actual practice of assisted reproductive technology, the cryopreservation of micro-sperm (≤10μL) is a crucial step in the treatment of male infertility. It is no exaggeration to say that the success or failure of this step directly affects the overall success of the treatment. However, current micro-sperm cryopreservation technologies have revealed some thorny problems in practical application, among which sample damage caused by insufficient physical protection is particularly prominent, causing significant headaches for clinicians.

[0004] Take traditional cryogenic carriers as an example. They have significant design flaws, lacking effective rigid protective structures. In liquid nitrogen storage environments or during transport operations, these carriers are highly susceptible to external pressure or impact. When placed in a liquid nitrogen tank, the low temperature of liquid nitrogen, combined with various operational activities within the tank, makes the cryogenic carrier easily come into contact with and be compressed by other objects. During transport, handling and moving operations inevitably cause collisions. Once subjected to external forces, the microfilters and other supporting components are easily deformed or even ruptured.

[0005] Such cases have been encountered in clinical practice. On one occasion, during the removal or placement of a cryogenic carrier from a liquid nitrogen tank, due to a lack of adequate physical protection, the carrier only slightly collided with other tools or the tank wall, causing the microfilter to rupture. This resulted in the immediate leakage of the sperm sample, ultimately leading to sample loss. All these situations ultimately cause sperm samples to lose their viability due to physical damage. This significantly impacts the success rate of assisted reproductive technologies and is a heavy blow to patients.

[0006] In addition, traditional cryopreservation media also have many problems with their connection structure and sealing. The connections between components are not robust enough, and they are prone to loosening and leakage when subjected to external forces or temperature changes. For example, the connections between the connecting tubes and protective sleeves, collection containers, and other components of some devices are not tightly sealed. During freezing or thawing, external substances may enter, or internal fluids may leak out, which will seriously affect the quality and preservation of sperm samples, significantly hindering our treatment efforts.

[0007] In assisted reproductive clinical practice, the challenges of cryopreservation of trace sperm are not limited to insufficient physical protection, unstable ligation, and poor sealing, but also include: Low sample recovery rate: Trace samples are prone to adhesion and loss during processing, resulting in insufficient number of effective sperm.

[0008] Operational complexity: Existing equipment has a cumbersome operating procedure and is prone to human error and pollution risks.

[0009] Poor traceability: The lack of effective sample identification and information management mechanisms is not conducive to long-term storage and large-scale sample management.

[0010] Therefore, we urgently need to improve existing micro-sperm cryopreservation devices due to their shortcomings in physical protection, such as the lack of rigid protective structures, unstable connection structures, and poor sealing. Developing a device that can effectively protect micro-sperm samples and improve the success rate of cryopreservation is of paramount practical importance to clinicians. Summary of the Invention

[0011] In view of this, the technical problem to be solved by this invention is how to solve the problem that existing micro-sperm cryopreservation devices are prone to deformation and breakage of the supporting components due to the lack of an effective rigid protective structure during liquid nitrogen storage and transportation, as well as the unstable connection structure and poor sealing performance, which in turn cause physical damage, leakage and quality loss of sperm samples, thus affecting the success rate of cryopreservation.

[0012] To achieve the above objectives, the present invention provides the following technical solution: A micro-sperm cryo-trapping device, comprising: A collection bucket; A hollow connecting pipe, one end of which is fixedly connected to the collection bucket; An elastic protective sleeve is provided to cover the outside of the collection bucket. One end of the elastic protective sleeve is fused to the hollow connecting pipe, and a connecting pipe is provided at the bottom of the elastic protective sleeve. An injection pump is connected to the other end of the hollow connecting tube; A mounting bracket for securing the injection pump; A protective cover, said protective cover being disposed over the elastic protective sleeve; and A locking mechanism is provided on the connecting section of the hollow connecting pipe to lock the port of the protective cover to the connecting section.

[0013] Furthermore, the engaging mechanism includes: A first rubber ring is fitted onto the connecting section; A second rubber ring is fitted around the outside of the first rubber ring, and the outer circumferential surface of the second rubber ring is tightly pressed against the inner wall of the port.

[0014] Furthermore, the first rubber ring includes: A connecting sleeve, the connecting sleeve being fitted onto the connecting segment; A first flange, the first flange being formed by extending radially outward from the outer periphery of the front end of the connecting sleeve; and A cantilever portion is formed by extending forward along the axial direction from the front end face of the first flange, and an annular notch is provided on the cantilever portion.

[0015] Furthermore, the notch includes a vertical wall and an inclined wall extending obliquely toward the central axis of the connecting sleeve.

[0016] Furthermore, the second rubber ring includes: A ring-shaped body, wherein the ring-shaped body is fitted over the first flange; A ring-shaped protrusion protrudes from the inner wall of the ring body and engages with the notch. A connecting arm is attached to the outer periphery of the front end of the ring body; An elastic arm extends from the end of the connecting arm in a direction away from the axis, with the outer end face of the elastic arm abutting against the inner wall of the port.

[0017] Furthermore, the annular protrusion includes: A shoulder surface, which abuts against the vertical wall; An inner conical surface abuts against the inclined wall.

[0018] Furthermore, the contact surface of the inclined wall is an arc-shaped surface that matches the inner conical surface.

[0019] Furthermore, the front edge of the inner hole of the connecting sleeve is chamfered.

[0020] Furthermore, the bottom wall of the protective cover has a through hole, through which the connecting tube at the bottom of the elastic protective sleeve passes.

[0021] Furthermore, removable plugs are provided at the outer ends of the two connecting pipes.

[0022] As can be seen from the above technical solution, the advantages of the present invention are: 1. By incorporating a protective cover and elastic protective sleeve, effective rigid protection is provided for the collection container and the sperm sample inside. During liquid nitrogen storage and transportation, it can withstand external pressure and collisions, preventing the deformation and breakage of the microfilter and other supporting components due to external forces. This effectively reduces the loss of sperm activity due to physical damage, improves the success rate of cryopreservation, and provides a more reliable sperm resource for assisted reproductive technologies.

[0023] 2. A locking mechanism is employed, with the cooperation of the first and second rubber rings to achieve a stable connection between the protective cover and the hollow connecting tube. This double-layer rubber sealing structure not only ensures a firm connection but also effectively prevents problems such as loosening of components or leakage caused by external forces or temperature changes, guaranteeing the overall stability and reliability of the device and ensuring the safety of sperm samples during cryopreservation.

[0024] 3. The locking mechanism and the sealing design of the connecting tube and plug, elastic protective sleeve and connecting tube effectively prevent external substances from entering the device and prevent internal liquid leakage. For example, the annular protrusion on the plug and the annular protrusion on the connecting tube enhance the sealing effect, avoid the impact of external contamination or liquid leakage on the quality of sperm samples, and improve the preservation effect of sperm samples.

[0025] 4. Appropriate materials are selected for each component, taking into full consideration factors such as biocompatibility, corrosion resistance, and low-temperature resistance. The microfilter of the collection tank is made of 316L stainless steel, which has good biocompatibility and corrosion resistance, and the surface is polished to avoid damaging sperm; the elastic protective sleeve is made of medical-grade silicone rubber, which is low-temperature resistant and soft and elastic; the hollow connecting tube is made of medical-grade 304 stainless steel and nickel-plated to enhance corrosion resistance, etc. These material selections ensure the safety and effectiveness of the device in assisted reproductive technology. Attached Figure Description

[0026] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0027] Figure 1 This is a schematic diagram of the main structure of the present invention.

[0028] Figure 2 This is a cross-sectional view of the protective cover of the present invention, including the connection between the connecting pipe and the through hole.

[0029] Figure 3This is a cross-sectional structural diagram showing the connection relationship between the port of the protective cover, the engaging mechanism, and the connecting segment of the present invention.

[0030] Figure 4 For the present invention Figure 3 A magnified view of a portion of point A.

[0031] Figure 5 For the present invention Figure 4 A magnified view of a portion of point B.

[0032] Figure 6 This is a schematic diagram of the collection bucket structure and the connection relationship between the conical part and the hollow connecting pipe of the present invention.

[0033] Figure 7 For the present invention Figure 6 A magnified view of a portion of point C.

[0034] Figure 8 This is a schematic diagram showing the positional relationship between the collection bucket and the elastic protective sleeve, as well as their connection with the hollow connecting pipe, according to the present invention.

[0035] Figure 9 For the present invention Figure 8 A magnified view of a portion of point D.

[0036] Figure 10 This is a schematic diagram showing the positional relationship between the left end of the elastic protective sleeve of the present invention, the connecting pipe, and the left end of the collection bucket.

[0037] Figure 11 For the present invention Figure 10 A magnified view of a portion of point E.

[0038] Figure 12 This is a schematic diagram of the blockage structure of the present invention.

[0039] Figure 13 This is a cross-sectional view of the annular protrusion of the blockage in this invention.

[0040] Figure 14 For the present invention Figure 11 A magnified view of a portion of point F.

[0041] Figure 15 This is a schematic diagram of the connecting pipe and the state of the hollow connecting pipe after sealing, according to the present invention.

[0042] Explanation of reference numerals in the attached figures: 1-Collection bucket; 11-Connecting pipe; 111-Flange; 112-Sealing section; 1121-Annular protrusion; 12-Plug; 121-Pull ring; 122-Through hole; 123-Connecting piece; 124-Insertion groove; 125-Connecting pin; 126-Sealing part; 1261-Annular protrusion; 13-Elastic protective sleeve; 131-Mounting hole; 14-Microfilter body; 15-Conical part; 3-Hollow connecting pipe; 31-Connecting section; 5-Mounting bracket; 6 - Injection pump; 7- Engaging mechanism; 71- First rubber ring; 7111- Connecting sleeve; 7111- Chamfer; 712- First flange; 713- Cantilever; 714- Notch; 7141- Vertical wall; 7142- Inclined wall; 72- Second rubber ring; 721- Ring body; 722- Annular locking protrusion; 7221- Shoulder surface; 7222- Inner conical surface; 723- Connecting arm; 724- Elastic arm; 9- Protective cover; 93- Through hole; 94- Port. Detailed Implementation

[0043] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the embodiments and accompanying drawings. Here, the illustrative embodiments and descriptions of this invention are used to explain the invention, but are not intended to limit the invention.

[0044] refer to Figures 1 to 15 ,like Figure 1 As shown in the figure, this embodiment provides a micro-sperm cryopreservation device for cryopreservation of micro-sperm in assisted reproductive technology. The specific structure, materials, connection methods and operation procedures of each component are described in detail below with reference to the accompanying drawings.

[0045] like Figure 1 , Figure 2 As shown, this embodiment provides a micro-sperm cryo-capture device. This micro-sperm cryo-capture device mainly consists of seven components: a collection container 1, a hollow connecting tube 3, an elastic protective sleeve 13, an injection pump 6, a mounting frame 5, a protective cover 9, and a locking mechanism 7, realizing the function of cryo-capturing micro-sperm. The collection container 1 is used to hold the sperm sample and is fixedly connected to the hollow connecting tube 3. The elastic protective sleeve 13 covers the outside of the collection container 1, and the small end of the elastic protective sleeve 13 is fused to the left end of the hollow connecting tube 3. Two connecting tubes 11 are provided at the bottom left end of the elastic protective sleeve 13. A through hole 93 is provided on the bottom wall of the left end of the protective cover 9 for the corresponding connecting tubes 11 to pass through. The connecting tubes 11 extend outward from the bottom wall of the protective cover 9 through the through hole 93. The protective cover 9 covers the outside of the elastic protective sleeve 13, providing physical protection.

[0046] In some embodiments, such as Figure 6 , Figure 7 , Figure 8 and Figure 9As shown, the collection container 1 is barrel-shaped. It consists of a microfiltration mesh body 14 and a conical portion 15. The collection container 1 is treated with a hydrophilic nano-coating or a bio-inert, low-adhesion material to minimize sperm adhesion and loss. The right end of the conical portion 15 is fixedly connected to the left end of the hollow connecting tube 3. The microfiltration mesh body 14 is made of microfiltration mesh, and its right end is welded to the left end of the conical portion 15. The pore size of the microfiltration mesh is 8-12 μm, with a default setting of 10 μm. The material is 316L stainless steel mesh.

[0047] The microfilter tank 14 is made of 316L stainless steel because 316L stainless steel has good biocompatibility and strong corrosion resistance. The surface is polished (Ra≤0.8μm) to avoid damaging sperm.

[0048] Specifically, the right end of the tapered portion 15 is provided with an external thread portion 151, and the external thread of the external thread portion 151 is threadedly connected to the internal thread 311 at the left end of the connecting section 31.

[0049] In some embodiments, such as Figure 2 As shown, the connecting pipe 11 and the through hole 93 are fitted with a clearance.

[0050] Preferably, such as Figure 1 , Figure 2 , Figure 8 As shown, there are two connecting pipes 11. The outer ends of the two connecting pipes 11 are fitted with removable plugs 12.

[0051] In some embodiments, such as Figure 10 , Figure 11 , Figure 12 and Figure 13 As shown, the plug 12 is made of silicone. The outer end of the plug 12 has an integrated area for a QR code or RFID chip compatible with automated label printing and scanning systems to achieve digital management of sample information. A pull ring 121 is provided at the outer end, with a through hole 122 in the center. A connecting piece 123 is connected to the right end of the pull ring 121. The connecting piece 123 is inserted into the insertion groove 124 of the plug 12 and connected to the plug 12 via a connecting pin 125. The right end of the plug 12 has a sealing part 126, which is sealed to the left end opening of the connecting pipe 11. To improve the reliability of the sealing connection, multiple annular protrusions 1261 are provided on the outer periphery of the sealing part 126, pressing against the inner wall of the left end opening of the connecting pipe 11.

[0052] Preferably, the cross-section of the annular protrusion 1261 is trapezoidal.

[0053] In some embodiments, such as Figure 11 , Figure 14As shown, the right end of the connecting pipe 11 has a flange 111, the left end face of the flange 111 can abut against the right end face of the mounting hole 131, the right end of the connecting pipe 11 also has a sealing section 112, the flange 111 is connected to the right end of the sealing section 112, and the sealing section 112 is sealed to the mounting hole 131.

[0054] Preferably, the outer periphery of the sealing section 112 is provided with a plurality of annular protrusions 1121, which abut against the inner wall of the mounting hole 131 to serve as a seal and a connection.

[0055] Preferably, the cross-section of the annular protrusion 1121 is semi-circular.

[0056] The hollow connecting tube 3 is a cylindrical tubular structure, with its two ends connected to the collection tank 1 and the infusion pump 6, respectively. The hollow connecting tube 3 is made of medical-grade 304 stainless steel, which is resistant to low temperatures and corrosion. The surface is plated with nickel (5μm thick) to enhance corrosion resistance.

[0057] In some embodiments, such as Figure 8 , Figure 9 , Figure 10 As shown, the elastic protective sleeve 13 is fused with the hollow connecting pipe 3, and two symmetrical mounting holes for connecting pipes 11 are opened along the axial direction at the left end, through which the connecting pipes 11 pass.

[0058] Preferably, the elastic protective sleeve 13 is made of medical-grade silicone rubber (Shore hardness 50A, resistant to low temperature -196℃ without hardening), and is sealed to the hollow connecting tube 3 by a hot melt process.

[0059] The syringe pump 6 is a miniature peristaltic pump (flow range 0.1-10mL / min). The pump head is connected to the hollow connecting tube 3 through a silicone hose, and the pump body is fixed on the mounting bracket 5 to ensure the stability of the syringe pump 6 during use.

[0060] The pump head of the syringe pump 6 is made of ceramic rotor (wear-resistant), the tubing is made of medical-grade silicone (good biocompatibility), and the mounting bracket 5 is made of ABS plastic.

[0061] In some embodiments, such as Figure 1 and Figure 2 As shown, the protective cover 9 is installed outside the elastic protective sleeve 13. The right end opening (port 94) of the protective cover 9 is connected to the locking mechanism 7, and two through holes 93 are opened in the center of the bottom wall of the left end. The through holes 93 are fitted with the connecting tube 11 of the elastic protective sleeve 13 with a clearance of 0.2mm.

[0062] The protective cover 9 is made of 304 stainless steel, and the edges of the through hole 93 are rounded with a radius of R0.5mm to prevent cuts.

[0063] In some embodiments, such as Figure 3 , Figure 4 and Figure 5 As shown, the locking mechanism 7 connects the port 94 of the protective cover 9 to the connecting section 31 of the hollow connecting pipe 3. The locking mechanism 7 is used to achieve quick locking and unlocking of the port 94 of the protective cover 9 and the connecting section 31. The locking mechanism 7 is a double-layer rubber sealing structure, including a first rubber ring 71 and a second rubber ring 72.

[0064] The connecting sleeve 711 of the first rubber ring 71 is a cylindrical sleeve with a 45° chamfer 7111 at the left end of the inner hole, 1mm deep, for easy installation. It is fitted onto the connecting section 31 of the hollow connecting pipe 3. The first flange 712 of the first rubber ring 71 is an annular boss extending from the outer periphery of the left end of the connecting sleeve 711.

[0065] The first rubber ring 71 has a cantilever portion 713, specifically, the outer end face of the first flange 712 is connected to the elastic cantilever portion 713, and the left end of the cantilever portion 713 has an annular notch 714, the notch 714 has a vertical wall 7141 and an inclined wall 7142.

[0066] The second rubber ring 72 has a ring body 721, an annular locking protrusion 722, a connecting arm 723, and an elastic arm 724. The ring body 721 has an annular structure and is fitted onto the first flange 712. The annular locking protrusion 722 is an annular protrusion extending inward from the inner wall of the ring body 721. Its shoulder surface 7221 abuts against the vertical wall 7141 of the notch 714, and its inner conical surface 7222 matches and fits against the arcuate surface of the inclined wall 7142 of the notch 714. The connecting arm 723 is an annular arm extending from the outer periphery of the ring body 721. The elastic arm 724 is connected to the upper end of the connecting arm 723, and its outer periphery tightly abuts against the inner wall of the port 94 of the protective cover 9, forming a radial tension force.

[0067] The notch 714 of the first rubber ring 71 and the annular locking protrusion 722 of the second rubber ring 72 engage bidirectionally through the conical surface and the vertical surface, providing strong axial tensile strength and preventing the protective cover 9 from accidentally detaching during operation.

[0068] The radial expansion of the elastic arm 724 enhances the friction of the inner wall of the port 94, providing double protection for sealing stability.

[0069] Installation of connecting tube 11 and plug 12: Pass two connecting tubes 11 (made of silicone) through the mounting holes 131 at the bottom of the elastic protective sleeve 13, and install the removable plug 12 (e.g., Figure 8 , Figure 10 and Figure 11 (As shown). The outer end of the connecting tube 11 is blocked by 12 to prevent refrigerant backflow and contamination of the sample.

[0070] Fixing the collection bucket 1 to the hollow connecting pipe 3: The right end of the tapered part 15 is provided with an external thread 151. The external thread of the external thread 151 is screwed into the internal thread 311 at the left end of the connecting section 31 (e.g., Figure 6 and Figure 7As shown in the image, the seal is achieved using PTFE tape to ensure no liquid leakage.

[0071] Installation of elastic protective sleeve 13: Insert the small right end of the elastic protective sleeve 13 into the left end of the connecting section 31 (where the conical part 15 is already fixed), and heat it with a hot air gun (around 150°C) to heat-melt and bond the elastic protective sleeve 13 to the hollow connecting pipe 3 (e.g., Figure 8 and Figure 9 As shown in the figure, a sealed connection is formed after cooling.

[0072] Assembly of the locking mechanism with the hollow connecting pipe 3: 1. Insert the connecting sleeve 711 of the first rubber ring 71 into the connecting section 31 of the hollow connecting pipe 3, and push the connecting sleeve 711 to the left to ensure that the connecting sleeve 711 completely covers the connecting section 31.

[0073] 2. Place the ring body 721 of the second rubber ring 72 onto the cantilever part 713, and push the ring body 721 to the left so that the annular locking protrusion 722 is engaged in the notch 714 of the cantilever part 713. At this time, the vertical wall 7141 is in close contact with the shoulder surface 7221, and the inclined wall 7142 is in contact with the arc surface of the inner conical surface 7222 to form a buckle lock.

[0074] When installing the protective cover 9, align the port 94 of the protective cover 9 with the elastic arm 724 of the second rubber ring 72, press the protective cover 9 so that the inner wall of the port 94 abuts against the elastic arm 724, and the elastic arm 724 abuts outward to complete the sealing connection.

[0075] Fixing the injection pump 6 to the mounting bracket 5: The peristaltic pump 6 is fixed to the pump groove of the mounting bracket 5 with bolts. The silicone hose of the pump head is connected to the right end of the hollow connecting pipe 3 through a Luer connector to ensure unobstructed liquid passage.

[0076] The process of capturing trace amounts of sperm: 1. Sample preparation: Take a small amount of sperm sample (volume ≤10μL) to be frozen and add it to the preservation solution (such as 10% glycerol + PBS buffer), and mix well.

[0077] 2. Capture and Loading: Start the syringe pump 6 and adjust the flow rate to 0.5 mL / min. Inject the sperm-protective solution mixture into the collection tank 1 through the hollow connecting tube 3. After being filtered through the microfiltration tank (pore size 10 μm), the mixture enters the annular chamber (thickness 2 mm) between the elastic protective sleeve 13 and the collection tank 1. The sperm are fixed on the surface of the microfiltration tank 14 or within the annular chamber.

[0078] 3. Sample Protection: Turn off the syringe pump 6, install the protective cover 9 on the locking mechanism 7, so that the elastic protective sleeve 13 and the collection container 1 are accommodated within the protective cover 9, remove the syringe pump 6, and quickly inject liquid nitrogen through the hollow connecting tube 3. Seal the right end opening of the hollow connecting tube 3 with the plug 12 (e.g., Figure 15(As shown), after adding a label, place it in a liquid nitrogen tank for storage.

[0079] If sperm revival is required, remove the frozen sperm from the liquid nitrogen tank, remove the plug 12 from the right end of the hollow connecting tube 3 and the plug 12 from the two connecting tubes 11, connect the injection pump 6 to the opening at the right end of the hollow connecting tube 3, blow away the liquid nitrogen, and quickly and circulate the revival fluid through the hollow connecting tube 3 and the two connecting tubes 11. After quickly reviving the sperm, blow away the revival fluid through the hollow connecting tube 3.

[0080] This implementation method fully realizes the efficient and safe capture of trace amounts of sperm, and is suitable for sperm cryopreservation scenarios in assisted reproductive technology.

[0081] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, various modifications and variations can be made to the embodiments of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A micro-sperm cryo-trapping device, characterized in that, include: One collection bucket (1); A hollow connecting pipe (3), one end of which is fixedly connected to the collection bucket (1); An elastic protective sleeve (13) is provided, which covers the outside of the collection bucket (1). One end of the elastic protective sleeve (13) is fused to the hollow connecting pipe (3). A connecting pipe (11) is provided at the bottom of the elastic protective sleeve (13). An injection pump (6) is connected to the other end of the hollow connecting tube (3); A mounting bracket (5) is used to fix the injection pump (6); A protective cover (9) is provided over the elastic protective sleeve (13); and A locking mechanism (7) is provided on the connecting section (31) of the hollow connecting pipe (3) for locking the port (94) of the protective cover (9) with the connecting section (31).

2. The micro-sperm cryo-capture device according to claim 1, characterized in that, The engaging mechanism (7) includes: A first rubber ring (71) is fitted onto the connecting section (31); A second rubber ring (72) is fitted on the outside of the first rubber ring (71), and the outer peripheral surface of the second rubber ring (72) is tightly against the inner wall of the port (94).

3. The micro-sperm cryo-capture device according to claim 2, characterized in that, The first rubber ring (71) includes: A connecting sleeve (711) is fitted onto the connecting segment (31). A first flange (712) is formed by extending radially outward from the outer periphery of the front end of the connecting sleeve (711); and A cantilever portion (713) is formed by extending forward along the axial direction from the front end face of the first flange (712), and an annular notch (714) is provided on the cantilever portion (713).

4. The micro-sperm cryo-capture device according to claim 3, characterized in that, The notch (714) includes a vertical wall (7141) and an inclined wall (7142) extending obliquely toward the central axis of the connecting sleeve (711).

5. The micro-sperm cryo-capture device according to claim 4, characterized in that, The second rubber ring (72) includes: A ring body (721) is fitted over the first flange (712); A ring-shaped protrusion (722) protrudes from the inner wall of the ring body (721) and engages with the notch (714); A connecting arm (723) is connected to the outer periphery of the front end of the ring body (721); An elastic arm (724) extends from the end of the connecting arm (723) in a direction away from the axis, and the outer end face of the elastic arm (724) abuts against the inner wall of the port (94).

6. The micro-sperm cryo-capture device according to claim 5, characterized in that, The annular protrusion (722) includes: A shoulder surface (7221) abuts against the vertical wall (7141); An inner conical surface (7222) abuts against the inclined wall (7142).

7. The micro-sperm cryo-capture device according to claim 6, characterized in that, The contact surface of the inclined wall (7142) is an arc-shaped surface that matches the inner conical surface (7222).

8. The micro-sperm cryo-capture device according to claim 3, characterized in that, The front edge of the inner hole of the connecting sleeve (711) is chamfered (7111).

9. The micro-sperm cryo-trapping device according to claim 3, characterized in that, The bottom wall of the protective cover (9) has a through hole (93), and the connecting tube (11) at the bottom of the elastic protective sleeve (13) passes through the through hole (93).

10. The micro-sperm cryo-trapping device according to claim 9, characterized in that, The two connecting pipes (11) are provided with removable plugs (12) at their outer ends.

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