7 results about "Pyridoxamine" patented technology

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.

The present disclosure relates to a non-therapeutic use of a human milk oligosaccharide (HMO) selected from 3'-sialyllacto-N-tetraose (LSTa), 6'-sialyllacto-N-neotetraose (LSTc) and 3'-sialyl- 3-fucosyllactose (FSL), or a combination thereof, in supporting or improving one or more of brain health, immune system health, gut health, and metabolic health, in a subject, optionally by increasing the production of one or more metabolites selected from the group consisting of of pyridoxine, pyridoxamine, nicotinic acid, N-acetyl-L-glycine, sarcosine, N-acetyl-L-glutamine, N- acetyl-L-aspartic acid, trigonelline, trans-4-hydroxyproline, 3-hydroxybutyric acid (BHB), 4- hydroxybenzoic acid, and N-acetyl-L-tryptophan, in the gastrointestinal tract of the subject.

The application provides application of pyridoxamine in sperm of oligospermia, preferably and / or in a freezing and resuscitation operation, pyridoxamine is added in a sperm operation liquid or a semen freezing liquid, and the following shortcomings of patients with oligospermia are effectively improved: (1) the ratio of sperm forward motility; (2) the ratio of sperm forward motility after semen freezing and resuscitation; (3) the sperm DNA fragmentation rate after semen freezing and resuscitation; (4) the sperm acrosome enzyme content after semen freezing and resuscitation; in summary, pyridoxamine can be added in the sperm operation liquid or the semen freezing liquid as a natural semen protective agent, the sperm selection treatment and the freezing and resuscitation effect of patients with oligospermia are obviously improved, and the application is suitable for application of patients with oligospermia in assisted reproductive treatment and fertility preservation.

The invention discloses a pyridoxamine derivative as well as a preparation method and application thereof, the pyridoxamine derivative has a structural general formula shown in the specification, and a substituent R is selected from ferulic acid, retinoic acid or nicotinic acid; the preparation method comprises the following steps: S1, dissolving a carboxylic acid compound in a solvent, adding a condensing agent and an activating agent, and stirring at 0 DEG C until the mixture is completely clarified and dissolved to obtain a reaction solution A; s2, dropwise adding alkali into the reaction liquid at the temperature of 0 DEG C, and reacting at the temperature of 0 DEG C to obtain reaction liquid B; s3, adding pyridoxamine hydrochloride into the reaction liquid B, and stirring and reacting for 12-48 hours at room temperature; and S4, after the pyridoxamine hydrochloride completely reacts, quenching the reaction with a saturated sodium bicarbonate aqueous solution, stirring and filtering a reaction solution, washing an organic phase with a saturated saline solution, extracting, collecting the organic phase, drying and concentrating, and purifying by column chromatography to finally obtain the target pyridoxamine derivative. The preparation method has the advantages of convenience in preparation and relatively low cost, and can be applied to preparation of cosmetics with anti-sugar, anti-oxidation and anti-aging effects.

The invention relates to the technical field of biological cell culture media, and particularly discloses a culture medium additive for CHO cell culture and a method for seed recovery, amplification and passage of CHO cells cultured in vitro through an improved culture medium. The additive is prepared from glucosamine, 1, 4-diaminobutane dihydrochloride, pyridoxamine, pyridoxine, pyridoxal, N-acetylcysteine, histidine and leucine. The use method comprises the following steps: grouping the substances, respectively preparing high-concentration mother liquor, and integrating the high-concentration mother liquor to a basic amp; in a fed culture medium, the additives are combined according to different concentrations in the cell culture process to achieve the effect of remarkably reducing G1F + G2F in antibody glycosylation.

The application relates to the field of biotechnology, and particularly relates to a method for obtaining a biological marker of a buffalo estrus period and application of the biological marker to identifying the buffalo estrus period, S1, collecting milk samples of a female buffalo, and observing follicle development of the female buffalo by using B ultrasonic waves; S2, after the milk samples are extracted, analysis is conducted by using a quadrupole-electric field orbitrap high-resolution mass spectrometry system and an ultrafast high-performance liquid chromatography system; S3, after original data are converted into an mzXML format, extraction data are obtained by using XCMS software processing, the extraction data are processed and analyzed, and significant difference metabolites of cow milk in an estrus period are screened out; S4, expression amounts of the significant difference metabolites of the cow milk in the estrus period and a non-estrus period are detected, the expression amount changes are compared with B ultrasonic wave results, and a biological marker of the buffalo estrus period is obtained. Compared with the prior art, pyridoxamine and homovanillic acid are obtained as the biological marker of the estrus period of the female buffalo, and are used for identifying whether the female buffalo is in the estrus period.

This invention relates to the non-therapeutic use of one or more lipids for stimulating the gut microbiota to produce certain vitamins. The invention also relates to a nutritional supplement product comprising one or more lipids for stimulating the gut microbiota to produce certain vitamins. Lipids of particular interest are lauric acid (C12:0), oleic acid (C18:1), and linoleic acid (C18:2), and vitamins of particular interest are riboflavin and pyridoxine.