40 results about "Sperm Preservation" patented technology

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

The invention discloses a fish sperm preserving fluid which is prepared from 2.120-2.420g of NaCl, 1.602-2.202g of KCL, 0.178-0.478g of CaCl2, 0.133-0.333g of MgSO4.7H2O, 0.114-0.414g of NaHCO3, 0.128-0.328g of VC and 500mL of distilled water. The fish sperm preserving method comprises the following steps of: (a) sperm collection: wiping and drying the genital pore region of a mature milter by using a towel, slightly pressing the abdomen of the mature milter, and collecting the extruded sperm by using a suction tube; and (b) sperm preservation: putting the collected sperm into a clean container, adding the sperm preserving fluid of which the volume is 10-20 times of the volume of the sperm, and preserving in low-temperature light-tight places.

The invention discloses a cryogen for Pacific cod sperm preservation, which is characterized in that: the cryogen is mixed with a freeze diluent and an antifreeze in a ratio of 9:1 (volume ratio), wherein the components of the freeze diluent The contents are: NaCl: 42-48mM, KHCO3: 2.5-3.5mM, CaCl2: 2.5-3.5mM, Glucose: 35-45mM, Tris: 1.5-2.5mM, Glutathione: 1.5-2.5mM, Bovine Serum Albumin: 1.5-2.5g / L, the pH value range of the frozen diluent is: 6.7-6.9; the antifreeze agent in the frozen agent is dimethyl sulfoxide (DMSO). As well as a method for cryopreserving Pacific cod sperm by using the cryogen. The freezing agent and the preservation method can preserve Pacific cod sperm for a long time, and can ensure that the sperm viability can still reach more than 80% after thawing.

The invention discloses a preservation solution, an activation solution and a hatching method for Paramisgurnus dabryanus sperms, belonging to the technical field of aquaculture. The sperm preservation solution comprises the following components: 0.5% to 1.0% of trisodium citrate, 1.0% to 3.0% of glucose, 0.1% to 0.3% of sodium bicarbonate, 0.02% to 0.04% of potassium chloride and 0.01% to 0.02% of VC; the activation solution comprises the following components: 1 to 2% of glucose and 0.35% of sodium chloride; and the hatching method comprises the following steps: activating sperms preserved with the sperm preservation solution by using the activation solution, allowing the sperms to combine with eggs, placing fertilized eggs on a mesh, and carrying out hatching in static water. Compared with the prior art, the invention has the following advantages: through innovation of the activation manner of the Paramisgurnus dabryanus sperms in a specific sperm preservation solution, the maximum vitality of the sperms can be guaranteed; the fertilization rate and the hatching rate of fish eggs are improved; and the fry breeding production is guided.

The invention discloses a Ptychidio jordani sperm cryopreservation method, which comprises four steps, namely semen collection, semen dilution, sperm cryopreservation, and sperm thawing. The semen ofPtychidio jordani is collected through an artificial abdomen squeezing manner, is then mixed with a diluent, and is mixed with an antifreeze agent to obtain a sperm preservation solution, with the diluent being one of a D-17 diluent, a Hank's solution and a Ringer's solution for fish, and the antifreeze agent being one of methanol, dimethyl sulfoxide and dimethyl sulfoxide; then the sperm preservation solution is injected into a cryopreservation tube, and the tube is immersed into liquid nitrogen for long-term storage; when to be in use, the cryopreservation tube containing the sperm preservation solution is taken out of the liquid nitrogen, and then immediately thaw in a water bath with a temperature of 37 DEG C, and the obtained Ptychidio jordani sperms are used for artificial insemination. The method can store the sperms of the Ptychidio jordani for long time and ensures the survival rates of sperms after thawing and resuscitation.

The invention discloses a method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms. The method comprises the steps that siniperca scherzeri seminal fluid is taken and freeze-preserved with sperm preservation liquid and then is irradiated by ultraviolet light after being diluted, and sperms are subjected to genetic inactivation; the inactivated sperms and siniperca chuats eggs are subjected to mixed fertilization, and egg gynogenesis is started; the eggs are transferred into fresh water for cold shock treatment after being fertilized, genomes are doubled, and heterogenous gynogenesis diploid is generated; the siniperca chuats gynogenesis diploid is transferred into culture and incubation water, incubation is carried out at room temperature, management is carried out according to a common siniperca chuats fry culture method, and siniperca chuats gynogenesis diploid fry are obtained. The siniperca chuats offspring generated through inducing of the method can accelerate excellent gene homozygosis, excellent inheritable characters can be fast fixed, and the disease resisting capacity is remarkably enhanced. According to the method, the conditions are easy to obtain, full-female siniperca chuats groups with the growth speed about 20% higher than that of common male siniperca chuats can be obtained later, and high breeding potential and application value are achieved.

The invention discloses an ancherythroculter nigrocauda sperm preservation method. The ancherythroculter nigrocauda sperm preservation method comprises the steps that 1, a sperm preserving fluid is prepared: raw material components are weighed in proportion and are respectively dissolved by using distilled water and are mixed evenly, and the distilled water is added to achieve volume fixing; 2, sperm acquisition is performed: an adult ancherythroculter nigrocauda milter is toweled off, and urine and faeces are removed to avoid sperm pollution; the abdomen is pressed slightly, extruded-out sperm is collected by using a suction tube, and sunlight or ultraviolet irradiation is avoided in the acquisition process; 3, sperm storage is performed: acquired ancherythroculter nigrocauda sperm is arranged in a dry, clean and sealed container subjected to sterilization and disinfection, and the container containing the sperm and the preserving fluid is kept in a low-temperature light-avoiding mode after the acquisition is completed. By the adoption of the technical scheme, the in-vitro survival time of the ancherythroculter nigrocauda sperm can be effectively prolonged, the fertilization vitality of the sperm can be kept, and protective development and distant crossbreeding of ancherythroculter nigrocauda are achieved. The ancherythroculter nigrocauda sperm preservation method is low in cost, simple and short in process and easy to operate, enables the preserving time to be long and has important application value.

A dairy cow sperm cryopreservation method relates to sperm preservation, in particular to a milk cow sperm cryopreservation method which has a protective effect on dairy cow sperm freezing and can improve the freezing effect. The cow sperm cryopreservation method is characterized in that the preservation method is to put the cow sperm into a low-temperature cryopreservation solution added with dove egg yolk for low-temperature preservation, and utilize the low-density lipoprotein contained in the dove egg yolk to inhibit the cow sperm Protective effect, reducing mortality in dairy cows after semen recovery. The dairy cow sperm cryopreservation method of the present invention adopts pigeon egg yolk to prepare, and pigeon egg yolk has a better protective effect on dairy cow sperm. Under this cryopreservation solution, dairy cow frozen sperm recovery motility, motility recovery rate and sperm quality The membrane integrity rate is higher than other cryopreservation solutions that have been reported to add different species of yolk; the use of this preservation solution can improve the freezing effect of dairy cow semen, and it is easy to popularize in practical production.

The invention belongs to the technical field of fish reproduction and discloses a method for reproducing snakehead fishes by using artificial insemination and a hatching barrel. The method comprises the following steps: temporarily rearing selected parent fishes in a cement pond at the water temperature of 25-26 DEG C and performing spawning induction; in the cement pond at the water temperature of 28-29 DEG C, after the effect time, carrying out inspection and ore squeezing once every 2 h, three times in total; temporarily rearing parent fishes after ore squeezing, preventing inflammation with a penicillin agent to keep the survival rate of female parent fishes at 75% or above; taking testes from male fishes, and grinding the testes in a sperm preservation solution in a separated manner with a metal net; and before fertilization, activating sperm in the sperm preservation solution, then pouring ores for fertilization, hatching the ores in the hatching barrel after leaving the ores tostand for more than 2 h, and inflating the hatching barrel; taking out fertilized ores after hatching the ores in the barrel for 2 days, and performing fry culture after yolk sacs are absorbed 1-2 days later. The method significantly the utilization rate of female parent fishes and the survival rate of the female parent fishes after spawning, reduces field occupation and increases the fry hatchingsuccess rate.

The invention provides a hybrid snakehead spermatozoa preserving fluid. Water is taken as solvent, and every one liter of preserving fluid comprises 7.5-8.5g of sodium chloride, 0.4-0.5g of potassium chloride, 0.1-0.15g of calcium chloride, 0.2-0.6g of sodium bicarbonate, 8-15g of glucose and the balance being water. The hybrid snakehead spermatozoa preserving fluid is simple in source, easy to operate and feasible, preservation is carried out at conventional low temperature, spermatozoa preserved with the preserving fluid are long in preservation life, high in activation rate and good in sperm motility, guarantee is provided for spermatozoa preservation and reproduction of hybrid snakehead, and the hybrid snakehead spermatozoa preserving fluid has good application prospect.

The invention relates to the field of fish sperm preservation solutions, in particular to a pseudobagrus sperm preservation solution for solving the problem of pseudobagrus large scale reproduction and a preparation method of the pseudobagrus sperm preservation solution. The sperm preservation solution is prepared from sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, glucose and penicillin and is prepared by weighing the raw materials, adding the raw materials in a plastic bottle or a glass bottle, and preparing into a solution with distilled water. The prepared sperm preservation solution is subjected to sperm chromatin detection with a flow cytometer, spermatids are circled on an FSC / SSC graph, and a door is set on an FL1(FITC) / FL3(ECD) scatter diagram for analysis; and a DNA fragment index at is calculated through a formula to evaluate sperm DNA quality. The sperm preservation solution provided by the invention can be preserved for 10 days in a refrigeration tank at the temperature of 4 DEG C, 98 percent or more of sperms keep exuberant vigor and can still be taken as sperms for semination, and the fertilization rate is 95 percent orabove.

The invention provides a cryopreservation method of grouper sperms, and belongs to the technical field of sperm preservation. The cryopreservation method comprises raw materials in parts by weight asfollows: 32-52 parts of NaCl, 28-57 parts of KCl, 21-40 parts of CaCl2, 20-61 parts of trehalose, 18-44 parts of liquid nitrogen, 20-68 parts of an anti-freezing liquid and 26-50 parts of compound antibiotics. By combination with the cryopreservation method comprising steps of sperm collection, dilution, precooling and quick-freezing for low-temperature storage of a seminal fluid, the reactivatedsperms can keep vigor of fresh sperms by the aid of a reasonable cooling rate, and the cryopreservation method has great significance for establishment of a marine fish sperm bank and protection for germplasm resources and biodiversity.

The invention discloses a sperm cryopreservation method of scatophagus argus. The sperm cryopreservation method comprises four steps of sperm collection, sperm dilution, sperm cryopreservation and sperm thawing, firstly male scatophagus argus is selected to inject human chorionic gonadotropin into dorsal muscle, then the sperm of the scatophagus argus is collected by an artificial abdominal extrusion method, then the sperm and antifreezing liquid are mixed to obtain preservation liquid of the scatophagus argus sperm, a diluent is prepared by mixing any one of a TS-2 diluent, a TS-19 diluent, aHank's solution, a Cortland diluent and sterilized seawater with dimethylsulfoxide; the sperm preservation liquid is injected into a cryopreservation tube, then freezing is conducted by adopting a step-by-step cooling method, and then the cryopreservation tube is immersed in liquid nitrogen for long-term preservation; when taking for use, the cryopreservation tube containing the sperm preservation liquid is taken out from the liquid nitrogen, and then is placed in a water bath with the temperature of 42 DEG C. According to the sperm cryopreservation method, the sperm of the scatophagus arguscan be preserved for a long time, and the sperm survival rate and insemination rate after thawing and resuscitation are high.

The invention relates to an ultralow-temperature cryopreservation method for fenneropenaeus chinensis sperms, and belongs to the technical field of sperm preservation. The method specifically comprises the following steps: diluting collected fenneropenaeus chinensis semen with an ultralow-temperature freezing protection solution, performing programmed cooling to-180 DEG C, and transferring into liquid nitrogen for preservation. According to the method, the fenneropenaeus chinensis semen diluted by the ultralow-temperature freezing protection liquid is subjected to programmed cooling to-180 DEG C and then is transferred into liquid nitrogen for preservation, the sperm stops metabolic activity in an ultralow-temperature environment so as to prolong the service life. The ultralow-temperature freezing protection liquid is combined with water molecules in the solution to initiate the hydration action. The crystallization process of water is weakened to increase the viscosity of the solution so as to reduce the formation of ice crystals. Meanwhile, a certain molar concentration can be maintained inside and outside cells to reduce the concentration of electrolyte in an unfrozen solution inside and outside the cells, so that the cells are prevented from being damaged by solutes, and the cells are protected from being damaged by the solution and the ice crystals.

The invention provides a boar sperm preservative as well as a preparation method and application thereof, and belongs to the technical field of livestock breeding. The boar sperm preservative provided by the invention is prepared from glucose, sodium hydrogen carbonate, sodium citrate, EDTA, citric acid, trihydroxymethyl aminomethane, L-cysteine, levofloxacin, sodium carboxymethylcellulose, bovine serum albumin and Banqing toxin-removing oral liquid. Compared with a conventional diluent Zorlesco, the boar sperm preservative provided by the invention has a better preservation effect of boar sperms, can improve the antibacterial ability of boar semen, and prolongs the storage time of the boar sperms.

The invention discloses an ultralow-temperature cryopreservation method for Yellow River carp sperms. The method specifically comprises the following steps: preparing a sperm preserving fluid from anantifreeze agent methanol and a diluent; mixing the Yellow River carp seminal fluid and the sperm preserving fluid according to a volume ratio of 1: 5 in a cryopreservation tube, wherein the volume concentration of the antifreeze agent methanol in the mixed solution of the Yellow River carp semen and the sperm preservation solution is 17%; and then pre-cooling for 5 minutes at the temperature of 4DEG C, balancing for 12 minutes at the position 5 cm above the liquid nitrogen, putting into the liquid nitrogen and carrying out ultralow-temperature treatment for 1 day, whrein the diluent is prepared from the following raw materials in percentage by weight: 0.95 of NaCl, 0.05% of KCl, 1.5% of glucose and the balance of water. The ultralow-temperature cryopreservation technology for the YellowRiver carps is established, a technical guarantee is provided for establishment of a Yellow River carp sperm freezer and the technical support is provided for germplasm preservation and genetic breeding work of the Yellow River carps.

The invention relates to a method for efficiently using ricefield eel sperms. The method comprises the following steps: 1, preparing a sperm preservation solution and carrying out freezing preservation; 2, taking out the pre-frozen sperm preservation solution, and adding semen of male ricefield eel into the pre-frozen sperm preservation solution to obtain a sperm mixed solution; 3, checking spermmotility and density by using a microscope, and calculating the number of configurable ricefield eel eggs of the sperm mixed solution; and 4, diluting the sperm mixed solution according to the configurable number of the finless eel eggs and carrying out preservation. The invention designs a method for efficiently utilizing ricefield eel sperms, cost is saved, and the fertilization rate of ricefield eel is increased. Through use of the ricefield eel sperms by the method, the sperms can be preserved for 72 hours at a temperature of 4 DEG C and can be available at any time, and the fertilizationrate reaches 95% or above.

The invention discloses a low-temperature preservation diluent for semen of plectropomus leopardus, a preservation method and application, and belongs to the field of artificial breeding of plectropomus leopardus. The diluent comprises the following components in parts by weight: 3.5 parts of sodium chloride, 1.68 parts of sodium bicarbonate, 2 parts of potassium chloride, 2 parts of reduced glutathione, 0.44 part of glucose and 10 parts of fetal calf serum. And diluting the semen of the plectropomus leopardus and the diluent according to a dilution ratio of (1: 9)-(1: 99), and storing at 0-4 DEG C. According to the invention, a novel formula for preserving the semen of the plectropomus leopardus at the low temperature of 0-4 DEG C is confirmed and put forward for the first time, the formula has no toxic effect on sperms, the longest sperm preservation time can reach 72 hours, the fertilization rate and the hatching rate are still 71.54% and 61.84% after the sperms are preserved for 24 hours, and compared with fresh sperms, the sperm preservation time is effectively prolonged, the sperm motility rate is ensured, and the survival rate of the sperms is improved. The requirement of low-temperature short-term storage of sperms during artificial improved variety breeding of plectropomus leopardus can be met.

The invention discloses a procypris merus sperm cryopreservation method. The method comprises four steps of seminal fluid collection, seminal fluid dilution, sperm cryopreservation and sperm unfreezing, procypris merus seminal fluid is collected by adopting an artificial abdominal extrusion method, then the seminal fluid is mixed with a diluent, the mixture is mixed with antifreezing liquid, and sperm preservation liquid is obtained, and the diluent is any one of D-15 diluent, Hank's liquid and Ringer's Solution for fishes, wherein the antifreeze agent is any one of methanol, dimethyl sulfoxide and glycerol; the sperm preservation liquid is injected into a cryopreservation tube, and the cryopreservation tube immersed into liquid nitrogen for long-term preservation; when the sperm preservation liquid is taken, the cryopreservation tube filled with the sperm preservation liquid is taken out of the liquid nitrogen and then immediately placed in a water bath kettle at the temperature of 27DEG C or 37 DEG C or 47 DEG C to be unfrozen, and then obtained procypris merus sperms are used for artificial insemination. The method can preserve the procypris merus sperms for a long time, and the survival rate of the thawed and recovered sperms is high.

A sperm drying liquid and a sperm preservation method, relating to the field of biological cell preservation, the sperm drying liquid can simulate the environmental state of the sperm growth state, can provide the basic needs of the sperm to survive in vitro and maintain a certain metabolic activity, and can better Protect the sperm and improve the integrity of the sperm. The sperm preservation method provided by the present invention adopts centrifugation to remove the semen in the semen, then mixes it with the above-mentioned sperm drying solution, and finally uses the evaporation drying method to dry. The whole process is simple to operate and does not require complex freezing and drying equipment. The treated sperm can be stored for a long time while still maintaining a high integrity. After long-term storage, it can be combined with egg cells to obtain a high cleavage rate and blastocyst development rate, especially suitable for field collection and collection of wild animal semen.

The invention belongs to the technical field of fish reproduction, and discloses a method for breeding Channaidae fish by using artificial insemination and hatching tanks. Selected broodstock are temporarily raised in a cement pond with a water temperature of 25-26°C, and induced labor is carried out; In a cement tank with a water temperature of 28-29°C, after the effect time is reached, check the egg squeeze every 2 hours, and perform a total of 3 times; after the egg squeeze, the broodstock is kept temporarily, and penicillin is used to prevent inflammation, and the female broodstock survives more than 75% ; The male fish takes the testis, and grinds it with a metal mesh in the sperm preservation solution; before fertilization, the sperm in the sperm preservation solution is activated with water, and then poured into the eggs for fertilization. The eggs are fertilized and left to stand for more than 2 hours, then hatched in the hatching bucket, and the hatching bucket is inflated; the fertilized eggs are hatched in the bucket for 2 days to produce membranes, and after another 1-2 days, the yolk sac is completely absorbed, and the seedlings are cultivated. The invention significantly improves the utilization rate of male broodstock and the postpartum survival rate of female broodstock, reduces site occupation and improves the success rate of seed hatching.

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

The invention discloses a freezing protection solution of Plectropomus leopardus sperms. The freezing protection solution comprises 34.23g of mycose, 0.9L of fetal calf serum, and water added to the total volume of 1L; and the pH value and the osmotic pressure of the freezing protection solution are 7.45 and 324mOsmol / Kg respectively. The preservation method comprises the following steps: carrying out isopyknic mixing on a seminal fluid and the freezing protection solution in 2ml of a freezing tube, allowing the freezing tube with the obtained mixed solution to stay in a position 5cm higher than a liquid nitrogen surface for 3min, and directly putting the freezing tube with the mixed solution into liquid nitrogen. The freezing tube with sperms frozen in the liquid nitrogen is taken out, and is processed in a 37DEG C water bath for 1min in order to unfreeze. Compared with freezing solutions in the prior art, the freezing protection solution has the advantages of very low toxicity to the sperms, and provision of nutrients needed by the sperms.

The invention discloses a powder for pig sperm dilution and room temperature preservation and the preparation method and the application thereof. The powder for sperm dilution and room temperature preservation comprises the following components according to the weight portion: 26.0-30.0 of glucose, 0.8-2.0 of sodium bicarbonate, 6.0-8.0 of sodium citrate, 2.0-3.0 of EDTA, 2.5-3.5 of citric acid, 5.0-6.0 of Tris, 2.0-4.0 of Hepes and disinfectant. Diluent with pH value being 6.8-7.0 is obtained after the powder is dissolved by distilled water. The powder is applied to room temperature long-term pig sperm preservation or common or high-time pig sperm dilution; when the pig sperm is stored under 17 DEG C, the activity of the pig sperm is more than 0.7 after 5 days. The invention has simple prescription component, low cost, long pig sperm preservation time and good preservation effect and can effectively prolong the preservation time of the pig sperm, enlarge the utilization efficiency of a superior breed boar and meet the demands of pig raising.

Provided is a cryopreservation container that prevents confusion of specimens to be used for treatment and that is suitable for individual management of fertilized eggs and the like to be cryopreserved. IC tags are attached to a cryopreservation tank 61, a canister 51, a cane 41, and cryopreservation containers such as an ovum storage container 1 and sperm storage containers 71, 81. The cryopreservation containers may also have individual identification codes attached thereto. An ovum storage container 11 is configured by fitting an IC tag 12 from the rear end of a rod-like part 2 of a conventional ovum storage container 1. In addition, the IC tag 12 is formed by providing an inlet 15 inside a thin-walled pipe 14 made of a synthetic resin. The inlet 15 may be fixed at both ends by means offixing members 18, 18 composed of a nonconductive material, or may be integrally molded with a nonconductive synthetic resin material 20.

The invention discloses a method for cryopreservation of sperms from anoplopoma fimbria. The method comprises steps of acquisition of sperms, preparation of a culture medium, a cryopreservation process of the sperms and resuscitation and activation processes of the sperms, namely acquiring sperms from anoplopoma fimbria at sexual maturity by using an artificial anesthesia method; according to thesperm osmotic pressure of the anoplopoma fimbria, preparing a sperm culture medium; mixing the acquired sperms and the culture medium according to a volume ratio of 1:2, and further adding dimethyl sulfoxide which accounts for 10% of the volume of the mixed liquid so as to obtain a cryopreservation mixed liquid; and putting a uniformly mixed sperm cryopreservation mixed liquid sample into a refrigerator of 4 DEG C for 15 minutes, allowing the sample to stand for 5 minutes at the opening of a liquid nitrogen bottle, moving the sample downwards, further allowing the sample to stand for 5 minutesabove the liquid level of liquid nitrogen, further allowing the sample to stand for 5 minutes on the liquid level of the liquid nitrogen, and immersing the sample into the liquid nitrogen, and performing cryopreservation. By adopting the method disclosed by the invention, the anoplopoma fimbria sperm preservation operation can be convenient, efficient and effective, the anoplopoma fimbria spermscan be prevented from rapid deactivation or death in vitro, the preservation quality of the sperms can be improved, the preservation time can be prolonged, and the anoplopoma fimbria sperms can be available at any time in actual production.

The invention belongs to the technical field of animal breeding, and specifically relates to a variable-temperature preservation diluent for rabbit semen and a preparation method thereof. The diluentis prepared by mixing a solution A and a solution B. The solution A is prepared by dissolving 5.0-35.0 g of bis(2-hydroxyethyl)amino(trimethylol)methane, 0.5-5.0 g of citric acid and 0.01-0.05 g of kanamycin with sterilized water for injection, fixing a volume to 100 ml, and successively performing subpackaging, sealing and sterilizing at a low temperature. The solution B is a glucose injection with a concentration of 5%. The solution A and the solution B are evenly mixed according to a ratio of 1: 9 to 9: 1 so as to obtain the diluent, and the pH value of the diluent is 7.0 at a temperature of 12 DEG C. The diluent provided by the invention has the advantages that sperm preservation time reaches 96 to 120 hours in a variable-temperature environment of 5 to 20 DEG C; after is the semen ispreserved with the diluent for 72 hours, sperm motility is 0.6 or above and the conception rate of female rabbits is 80% or above. Meanwhile, the diluent is simple to prepare and store and convenientto use.

The invention discloses an ancherythroculter nigrocauda sperm preservation method. The ancherythroculter nigrocauda sperm preservation method comprises the steps that 1, a sperm preserving fluid is prepared: raw material components are weighed in proportion and are respectively dissolved by using distilled water and are mixed evenly, and the distilled water is added to achieve volume fixing; 2, sperm acquisition is performed: an adult ancherythroculter nigrocauda milter is toweled off, and urine and faeces are removed to avoid sperm pollution; the abdomen is pressed slightly, extruded-out sperm is collected by using a suction tube, and sunlight or ultraviolet irradiation is avoided in the acquisition process; 3, sperm storage is performed: acquired ancherythroculter nigrocauda sperm is arranged in a dry, clean and sealed container subjected to sterilization and disinfection, and the container containing the sperm and the preserving fluid is kept in a low-temperature light-avoiding mode after the acquisition is completed. By the adoption of the technical scheme, the in-vitro survival time of the ancherythroculter nigrocauda sperm can be effectively prolonged, the fertilization vitality of the sperm can be kept, and protective development and distant crossbreeding of ancherythroculter nigrocauda are achieved. The ancherythroculter nigrocauda sperm preservation method is low in cost, simple and short in process and easy to operate, enables the preserving time to be long and has important application value.

The invention relates to a method for efficiently creating heterooctaploid silver crucian carp. The method comprises an artificial insemination stage, wherein the artificial insemination stage comprises the following steps: preparing a trypsin : adding trypsin powder into a sperm preservation solution, and uniformly mixing to obtain the trypsin solution;adding 0.9-1.1 g of trypsin powder into every 100 mL of sperm preservation solution; treating semen: adding mature semen of the white crucian carp into the trypsin solution, and treating for 13-17 min at the temperature of 22-24 DEG C; insemination hatching: performing dry insemination on the treated semen and the mature eggs of the heterogenetic silver crucian carp, and hatching the obtained fertilized eggs to obtain the heterooctaploid silver crucian carp. The method comprises the following steps: treating spermatozoa by using trypsin solution dissolved in sperm preservation solution to decoagulate the spermatozoa so as to achieve thesuccessful integration of heterologous spermatozoa into the eggs of the heterogenous silver-bearing crucian carp to form heterologous octaploid; further obtaining the heterologous octaploid silver crucian carp with high proportion by setting proper trypsin concentration and treatment time.