13 results about "Sperm Preservation" patented technology

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

The invention relates to a low-temperature preservation method for sheep semen, and belongs to the technical field of animal reproduction. The invention provides a method for low-temperature preservation of sheep semen, which comprises the following steps: (1) collecting breeding male sheep semen by a false vagina method to obtain the collected sheep semen; (2) mixing the sheep semen collected in the step (1) with a semen diluent and fullerene to obtain a sheep semen diluent; and (3) preserving the sheep semen diluent under the condition of 2-8 DEG C. The sheep semen is preserved according to the method, the effective application time of sperm preservation can be remarkably prolonged, the blastocyst forming rate of in-vitro fertilization embryos can be remarkably increased, and the method is applied to reproduction technologies such as artificial insemination (AI) and in-vitro fertilization (IVF) of sheep, the utilization rate of high-quality breeding rams can be increased, and genetic resource protection and efficient breeding can be promoted.

The invention discloses a low-temperature preservation method of Octopus variabilis sperms and spermatophore, the Octopus variabilis sperms adopt an ultralow-temperature cryopreservation method, the Octopus variabilis sperms are protected by using a cryoprotectant composed of a 0.6 mol / L glucose solution diluent and 20% glycerol, the diluent contains nutrient substances, and the Octopus variabilis sperms have the characteristics of inactivation of sperms and small damage to sperms. The cryoprotectant has the functions of a permeable antifreeze agent and a non-permeable antifreeze agent, so that the sperms are subjected to dual protection in the freezing process; the octopus variabilis spermatophore is preserved at a low temperature, a 1.0 mol / L glucose solution is used as a spermatophore protection solution, the spermatophore structure can be maintained within one month, and high sperm activity of octopus variabilis sperms can be maintained within a short period (one month). Based on requirements in experiments, two octopus variabilis sperm preservation methods are provided and can be flexibly used, and technical support is provided for preservation of octopus variabilis germplasm resources and overcoming of the problem of asynchronous male and female development.

This utility model discloses a sperm cryopreservation tube for sealing, relating to the field of cryopreservation technology. The technical solution includes a tube body with symmetrically fixed blocks on its inner side. A cap is movably connected inside the tube body, and an inner sleeve is fixedly connected inside the cap. A pressing assembly is provided inside the inner sleeve, including a pressing pestle and a button. A sealing sheet is movably connected inside the cap, and a movable plate is fixedly connected to the outside of the sealing sheet. A second spring is fixedly connected to the outside of the movable plate, with one end of the second spring away from the movable plate fixedly connected to the inner wall of the cap. A groove is provided on the inner wall of the tube. By using the pressing assembly, the sealing sheet is pressed, causing it to slide on the inner sleeve and engage in the groove to seal the injection port of the tube body, preventing the cap from falling off and causing liquid nitrogen contamination. This simplifies the process of hand-held rotation when sealing the cap and tube body, quickly sealing the injection port of the tube body, preventing excessive air from entering the tube, and ensuring the sperm preservation effect.

The invention discloses a porcine epididymal cell-derived extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. By optimizing the preparation method of the extracellular vesicles, the extracellular vesicles with high purity and standard shape are successfully obtained. Furthermore, by virtue of a proteomics technology, the composition characteristics of rich key functional proteins related to oxidation resistance, movement regulation and fertilization are systematically clarified for the first time. Functional experiments prove that the vesicle can be effectively attached to sperms, the sperm motility is remarkably improved under the low-temperature preservation condition, the oxidation resistance is enhanced, membrane damage, mitochondrial dysfunction and DNA damage are relieved, and the specific protection effect on the sperms is shown. The invention provides a reliable material basis and theoretical basis for developing a novel sperm preservative and an assisted reproduction technology based on extracellular vesicles.

The present application relates to the field of animal reproduction technology, and in particular to a sperm protection agent for improving sperm preservation quality and embryo development potential and application thereof, wherein the effective components of the sperm protection agent are selected from any one or several of acetyl carnitine, glycerophosphocholine, tauroursodeoxycholic acid, glutamic acid, sunflower acylcarnitine, pyridoxal phosphate and glyco ursodeoxycholic acid. Based on the regulation of the OR4C13-G13 / Golf signal axis related to sperm capacitation of the effective components, sperm viability can be maintained during preservation, premature capacitation can be inhibited, and the fertilization rate and embryo development potential can be improved. The present application is suitable for the normal temperature preservation of pig semen and can be extended to other mammalian or human assisted reproduction fields.

The application provides a low-temperature preservation diluent for sperm of Epinephelus lanceolatus and a use method and application thereof. The diluent is prepared from the following raw materials: glucose, calcium chloride, potassium chloride, sodium chloride, sodium bicarbonate, reduced glutathione, sodium dihydrogen phosphate and fetal bovine serum. The diluent contains the following mass of raw materials per liter: 0.4-0.5 g of glucose, 0.03-0.08 g of calcium chloride, 0.15-0.25 g of potassium chloride, 3-4 g of sodium chloride, 1.5-2.0 g of sodium bicarbonate, 1.5-2.5 g of reduced glutathione, 0.008-0.012 g of sodium dihydrogen phosphate, and 8-12% of the fetal bovine serum in terms of the volume percentage of the diluent. The prepared diluent can better maintain the in-vitro activity of sperm, prolong the sperm preservation time, meet the low-temperature preservation requirements of the sperm of Epinephelus lanceolatus, and especially meet the low-temperature preservation requirements of the sperm of Epinephelus lanceolatus.

The application provides a method for cryopreservation and ultra-cryopreservation of the sperm of feed domesticated mandarin fish, and first provides a cryopreservation diluent for the sperm of the mandarin fish, which comprises Hanks solution, sodium chloride, soybean lecithin, vitamin E, curcumin, melatonin and SOD; an anti-freezing protective agent is added in the cryopreservation diluent to obtain an ultra-cryopreservation anti-freezing solution for the sperm of the mandarin fish; the sperm of the mandarin fish is mixed with the cryopreservation diluent to realize cryopreservation; and the sperm of the mandarin fish is mixed with the ultra-cryopreservation anti-freezing solution to realize ultra-cryopreservation. The cryopreservation diluent for the sperm of the mandarin fish can realize cryopreservation of the sperm of the mandarin fish, and the sperm can be ultra-cryopreserved after the addition of the anti-freezing protective agent, so that the persistence of the preservation time of the sperm of the mandarin fish is ensured, the protection effect on the sperm is good, the activation rate of the sperm after thawing is high, and the sperm has good vitality.

The invention discloses an ultralow-temperature rice field carp semen preserving fluid and a method for preserving and recovering rice field carp semen, and belongs to the technical field of rice field carp artificial breeding. The ultralow-temperature rice field carp semen preserving fluid comprises a diluent and an anti-freezing agent, the components of the diluent are glucose, KCl and tris (hydroxymethyl) aminomethane, and the solvent is pure water; or the components of the diluent are trehalose, KCl and tris (hydroxymethyl) aminomethane, and the solvent is pure water; the antifreeze agent comprises one or two of methanol or ethylene glycol. According to the technology, an optimized diluent formula and a specific anti-freezing agent are combined, and a centrifugal washing step after unfreezing is combined, so that long-term and efficient preservation of the carp semen in the rice field in liquid nitrogen at the temperature of-196 DEG C is successfully realized, and the sperm motility, the mitochondrial function and the fertilization ability are remarkably improved after unfreezing.

The invention provides a metabonomics-based mongolian sheep semen liquid preservation diluent and preparation thereof, the diluent comprises a basic diluent, an antibiotic, a membrane stabilizer and a differential metabolite, the basic diluent comprises a buffer component, an organic acid, sugar and water, the buffer component is Tris, and the organic acid is citric acid; the sugar is selected from one or more of fructose, glucose, cane sugar and lactose; the basic diluent contains 2-3 g of a buffer component, 1-2 g of organic acid, 1-3 g of sugar and the balance of distilled water according to the volume of 100 mL. The invention further provides application of the preservation diluent in Mongolian sheep semen preservation. The technical bottleneck of 17 DEG C liquid preservation of the Mongolian sheep semen is broken through, and the preservation period of the sperms is prolonged by supplementing a large amount of micromolecular substances consumed in the preservation process of the sperms screened in metabonomics.

The invention relates to the technical field of biological breeding, and discloses an application of a PDHB gene inhibitor in improving the preservation quality of frozen pig sperms.By means of the technology, exogenous miR-let-7a or mimics or analogues for inhibiting PDHB gene expression are added into frozen and preserved pig semen, so that the effects of improving the sperm motility and improving the semen quality are achieved; especially, indexes such as sperm motility, sperm mitochondrial ROS content, membrane potential and the like are obviously superior to those of all groups including a control group.

The invention relates to the technical field of biological breeding, and discloses an application of exogenous miR-92a or a simulant or an analogue in improving the preservation quality of fresh boar sperms.The exogenous miR-92a or the simulant or the analogue is added into the fresh boar sperm preserved at normal temperature through the technology, PI3K / AKT signal channels are activated by down-regulating expression of PTEN, and the preservation quality of the fresh boar sperm is improved. The effects of improving the sperm motility, improving the sperm plasma membrane intact rate and improving the semen quality can be achieved.

Anchovy sperm freezing preservation solution, and a preparation method and application method thereof, belong to the technical field of aquatic germplasm resource protection and breeding, and particularly relate to a method for collecting, preserving and recovering sperm of anchovy fish. The present application is to solve the problems of low efficiency, short preservation time and low sperm activity of the existing anchovy sperm collection, and the anchovy sperm freezing preservation solution is composed of methanol, lecithin, resveratrol and glucose, and is diluted with double distilled water to 1L. The application method is as follows: 1. sperm collection and quality evaluation; 2. preparation of anchovy sperm freezing preservation solution; 3. mixing the sperm with the pre-cooled 4 DEG C anchovy sperm freezing preservation solution, dispensing, and adopting a four-step method for programmed cooling; 4. oocyte collection; 5. sperm warming and activity detection; 6. artificial insemination and hatching. The sperm preservation time is prolonged from several hours to more than several years, and through rapid thawing, the sperm activity can reach more than 40%, and the fertilization rate can reach more than 57%.