28 results about "Sperm Preservation" patented technology

The invention discloses lactobacillus paracasei and belongs to the technical field of microorganisms. The lactobacillus paracasei is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, China, the preservation number is CGMCC No.26029, and the preservation date is November 2, 2022. The lactobacillus paracasei has the advantages that the lactobacillus paracasei can be used for preparing the lactobacillus paracasei, and the lactobacillus paracasei can be used for preparing the lactobacillus paracasei; the lactobacillus paracasei capable of effectively relieving constipation and preventing aging through the antioxidant function of the lactobacillus paracasei is obtained for the first time, and the lactobacillus paracasei has the advantages of being convenient to eat, simple in treatment, high in relieving rate and free of toxic and side effects.

The invention belongs to the technical field of biological medicines, and discloses a lactobacillus paracasei strain with an anti-hypoxia function, the name of the lactobacillus paracasei strain is 40509a, the classification name of the lactobacillus paracasei strain is lactobacillus paracasei, the preservation number of the lactobacillus paracasei strain is CGMCC No.31996, the preservation date of the lactobacillus paracasei strain is CGMCC No.31996, the preservation unit of the lactobacillus paracasei strain is China General Microbiological Culture Collection Center, and the preservation unit of the lactobacillus paracasei strain is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus paracasei 40509a can prolong the survival time of a CD-1 hypoxic mouse and inhibit the content of HIF1-alpha and PHD in serum of the hypoxic mouse so as to achieve the anti-hypoxic function, and can improve the activity of PC12 hypoxic cells and improve the expression of NSE and NGF of hypoxic cells so as to achieve the anti-hypoxic function.

The present invention discloses Pseudomonas WH‑3 and its application, belonging to the field of microbial technology. The Pseudomonas WH‑3, Latin name Pseudomonas juntendi WH‑3, is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a deposit date of August 2, 2024, a deposit number of CGMCC No.31535, and a deposit location in Beijing, China. The Pseudomonas WH‑3 provided by the present invention is separated and screened from seawater contaminated by metolachlor. The strain is adapted to a wide range of salinities, can efficiently degrade metolachlor and 2‑methyl‑6‑ethylaniline, and has an efficient bioremediation effect on marine environments polluted by metolachlor and 2‑methyl‑6‑ethylaniline.

The invention relates to the technical field of frozen sperms, in particular to a cattle sex control frozen sperm preservation method which comprises the following steps: collecting bull semen, and purifying the bull semen to obtain a sperm suspension so as to determine the qualification of the quality of the semen; performing sperm sorting on the sperm suspension by using a flow cytometry to separate target sperms based on a determination result that the quality of the sperm is qualified; uniformly mixing the target sperms with a refrigerating fluid, standing for a preset period of time, and freezing; obtaining a particle size distribution curve and an ROS concentration curve of the target sperm in the freezing process, and determining the qualification of the temperature drop rate according to the distribution proportion of the particle size distribution curve; adjusting the temperature drop rate according to a comparison result of an ROS fluctuation index of the ROS concentration curve and a preset index on the basis of a judgment result that the temperature drop rate is unqualified; and unfreezing and resuscitating the frozen target sperms to determine the sperm malformation rate so as to adjust the preset activity or the preset index. According to the invention, the quality of frozen sperms is improved.

The invention discloses a streptomyces strain S1 for preventing and controlling root rot, the streptomyces strain S1 is classified and named as Streptomyces peuceius, the Streptomyces peuceius is preserved in China General Microbiological Culture Collection Center on April 26, 2024, and the preservation number of the strain is CGMCC NO.30458. The invention further discloses a preparation method of the Streptomyces peuceius strain S1 for preventing and controlling root rot. The invention also discloses the application of the streptomyces strain S1 or the combined use of the streptomyces strain S1 and resource substances in prevention and control of root rot, and the resource substances are selected from at least one of methyl jasmonate and brassinolide.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

The invention relates to a low-temperature preservation method for sheep semen, and belongs to the technical field of animal reproduction. The invention provides a method for low-temperature preservation of sheep semen, which comprises the following steps: (1) collecting breeding male sheep semen by a false vagina method to obtain the collected sheep semen; (2) mixing the sheep semen collected in the step (1) with a semen diluent and fullerene to obtain a sheep semen diluent; and (3) preserving the sheep semen diluent under the condition of 2-8 DEG C. The sheep semen is preserved according to the method, the effective application time of sperm preservation can be remarkably prolonged, the blastocyst forming rate of in-vitro fertilization embryos can be remarkably increased, and the method is applied to reproduction technologies such as artificial insemination (AI) and in-vitro fertilization (IVF) of sheep, the utilization rate of high-quality breeding rams can be increased, and genetic resource protection and efficient breeding can be promoted.

The present invention provides a strain of Ochrobacterium HRKJ-3, a microbial preparation and an application thereof. The Ochrobacterium HRKJ-3 is deposited with CGMCC No. 24350. The strain was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on January 20, 2022, and the strain deposited with CGMCC No. 24350. The strain can simultaneously carry out heterotrophic nitrification and aerobic denitrification under aerobic conditions, has good application potential in simplifying the denitrification process, saving processing space and cost, and has good application prospects in the treatment of domestic sewage and the denitrification of livestock and poultry breeding sewage with high ammonia nitrogen concentration.

The invention discloses a low-temperature preservation method of Octopus variabilis sperms and spermatophore, the Octopus variabilis sperms adopt an ultralow-temperature cryopreservation method, the Octopus variabilis sperms are protected by using a cryoprotectant composed of a 0.6 mol / L glucose solution diluent and 20% glycerol, the diluent contains nutrient substances, and the Octopus variabilis sperms have the characteristics of inactivation of sperms and small damage to sperms. The cryoprotectant has the functions of a permeable antifreeze agent and a non-permeable antifreeze agent, so that the sperms are subjected to dual protection in the freezing process; the octopus variabilis spermatophore is preserved at a low temperature, a 1.0 mol / L glucose solution is used as a spermatophore protection solution, the spermatophore structure can be maintained within one month, and high sperm activity of octopus variabilis sperms can be maintained within a short period (one month). Based on requirements in experiments, two octopus variabilis sperm preservation methods are provided and can be flexibly used, and technical support is provided for preservation of octopus variabilis germplasm resources and overcoming of the problem of asynchronous male and female development.

The present invention belongs to the technical field of microbial wastewater treatment, and discloses a strain of Achromobacter denitrificans HRKJ-1, whose deposit number is CGMCC No.24348. The strain has been deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee on January 20, 2022, referred to as CGMCC, with an address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No.24348. The strain can achieve the simultaneous removal of ammonia nitrogen, total nitrogen and COD under aerobic conditions, and has the characteristics of acid and alkali resistance, salt resistance and high concentration of ammonia nitrogen resistance.

The invention relates to the technical field of insemination devices, and discloses an insemination device used for artificial insemination of Chinese and western bees, the insemination device comprises a spiral injector body, a cooling mechanism and a heating mechanism, the inside of the spiral injector body is fixedly connected with a storage tube, and the outer side of the spiral injector body is fixedly connected with a sleeve; a temperature sensor is arranged in the sleeve, the storage tube is located in the sleeve, a switching mechanism is arranged at the bottom of the sleeve, a needle tip is installed at the bottom of the switching mechanism, the cooling mechanism comprises a liquid carbon dioxide storage tank, and the liquid carbon dioxide storage tank is located outside the spiral injector body. And the outer side of the liquid carbon dioxide storage tank is fixedly connected with a conveying pipe. The temperature rising or cooling mechanism is flexibly started according to the room temperature, the temperature in the storage pipe is continuously adjusted to be kept in a constant-temperature state suitable for sperm preservation, a good preservation environment is provided for sperms, and therefore the insemination effect is improved.

This utility model discloses a sperm cryopreservation tube for sealing, relating to the field of cryopreservation technology. The technical solution includes a tube body with symmetrically fixed blocks on its inner side. A cap is movably connected inside the tube body, and an inner sleeve is fixedly connected inside the cap. A pressing assembly is provided inside the inner sleeve, including a pressing pestle and a button. A sealing sheet is movably connected inside the cap, and a movable plate is fixedly connected to the outside of the sealing sheet. A second spring is fixedly connected to the outside of the movable plate, with one end of the second spring away from the movable plate fixedly connected to the inner wall of the cap. A groove is provided on the inner wall of the tube. By using the pressing assembly, the sealing sheet is pressed, causing it to slide on the inner sleeve and engage in the groove to seal the injection port of the tube body, preventing the cap from falling off and causing liquid nitrogen contamination. This simplifies the process of hand-held rotation when sealing the cap and tube body, quickly sealing the injection port of the tube body, preventing excessive air from entering the tube, and ensuring the sperm preservation effect.

The application discloses a preparation method of a normal-temperature preservation diluent for pig semen, and belongs to the technical field of biology.The main components of the normal-temperature preservation diluent are glucose, citric acid, sodium citrate, sodium bicarbonate, tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid disodium salt, penicillin G sodium salt, streptomycin sulfate, arbutin and double-distilled water.The diluent can maintain high activity of pig sperm in a short period, and the normal-temperature preservation time can reach about 10 days and meet the requirements of artificial insemination.The arbutin of the diluent has good anti-inflammatory and antioxidant protection effects, effectively improves the preservation activity and effect of pig sperm, and can be directly used for insemination after dilution or used for insemination after short-term preservation and short-distance transportation after dilution.The diluent improves the preservation quality of pig semen by using a low production cost and a simple operation method, is helpful to improve the utilization rate of excellent breeding stock, improve the reproduction rate, and is convenient for popularization and application.

The invention discloses a porcine epididymal cell-derived extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. By optimizing the preparation method of the extracellular vesicles, the extracellular vesicles with high purity and standard shape are successfully obtained. Furthermore, by virtue of a proteomics technology, the composition characteristics of rich key functional proteins related to oxidation resistance, movement regulation and fertilization are systematically clarified for the first time. Functional experiments prove that the vesicle can be effectively attached to sperms, the sperm motility is remarkably improved under the low-temperature preservation condition, the oxidation resistance is enhanced, membrane damage, mitochondrial dysfunction and DNA damage are relieved, and the specific protection effect on the sperms is shown. The invention provides a reliable material basis and theoretical basis for developing a novel sperm preservative and an assisted reproduction technology based on extracellular vesicles.

The present invention discloses Paenibacillus contaminans WH-2 and its application, belonging to the field of microbial technology. The Latin name of Paenibacillus contaminans WH-2 is Paenibacillus contaminans WH-2, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a deposit date of April 10, 2024, a deposit number of CGMCC No.30299, and a deposit address of Beijing, China. Paenibacillus WH-2 provided by the present invention is separated and screened from marine shellfish in the sea area contaminated by isopropyl metolachlor. The strain is adapted to a wide range of salinities, can efficiently degrade isopropyl metolachlor and 2-methyl-6-ethylaniline, and has an efficient bioremediation effect on the marine environment contaminated by isopropyl metolachlor and 2-methyl-6-ethylaniline.

The present application relates to the field of animal reproduction technology, and in particular to a sperm protection agent for improving sperm preservation quality and embryo development potential and application thereof, wherein the effective components of the sperm protection agent are selected from any one or several of acetyl carnitine, glycerophosphocholine, tauroursodeoxycholic acid, glutamic acid, sunflower acylcarnitine, pyridoxal phosphate and glyco ursodeoxycholic acid. Based on the regulation of the OR4C13-G13 / Golf signal axis related to sperm capacitation of the effective components, sperm viability can be maintained during preservation, premature capacitation can be inhibited, and the fertilization rate and embryo development potential can be improved. The present application is suitable for the normal temperature preservation of pig semen and can be extended to other mammalian or human assisted reproduction fields.

The application provides a low-temperature preservation diluent for sperm of Epinephelus lanceolatus and a use method and application thereof. The diluent is prepared from the following raw materials: glucose, calcium chloride, potassium chloride, sodium chloride, sodium bicarbonate, reduced glutathione, sodium dihydrogen phosphate and fetal bovine serum. The diluent contains the following mass of raw materials per liter: 0.4-0.5 g of glucose, 0.03-0.08 g of calcium chloride, 0.15-0.25 g of potassium chloride, 3-4 g of sodium chloride, 1.5-2.0 g of sodium bicarbonate, 1.5-2.5 g of reduced glutathione, 0.008-0.012 g of sodium dihydrogen phosphate, and 8-12% of the fetal bovine serum in terms of the volume percentage of the diluent. The prepared diluent can better maintain the in-vitro activity of sperm, prolong the sperm preservation time, meet the low-temperature preservation requirements of the sperm of Epinephelus lanceolatus, and especially meet the low-temperature preservation requirements of the sperm of Epinephelus lanceolatus.

The present invention belongs to the field of environmental microbiology, and specifically relates to a surfactant-producing petroleum-degrading bacterium KMJ-1 and its use. The technical points are as follows: the present invention is provided in the General Biological Center of the China Microbiological Culture Collection Committee, Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the deposit number is CGMCC No.21171. The surfactant-producing petroleum-degrading bacterium KMJ-1 provided by the present invention has the functions of producing surfactants and degrading petroleum. The strain has a strong ability to produce surfactants and can reduce the surface tension of pure water from 72.01 mN / m to 39.53 mN / m; the degradation rate of petroleum hydrocarbons in scum degraded by KMJ-1 strain is up to 80.06%; the degradation rate of petroleum hydrocarbons in biochemical sludge degraded by KMJ-1 strain is up to 85.88%.

The application provides a method for cryopreservation and ultra-cryopreservation of the sperm of feed domesticated mandarin fish, and first provides a cryopreservation diluent for the sperm of the mandarin fish, which comprises Hanks solution, sodium chloride, soybean lecithin, vitamin E, curcumin, melatonin and SOD; an anti-freezing protective agent is added in the cryopreservation diluent to obtain an ultra-cryopreservation anti-freezing solution for the sperm of the mandarin fish; the sperm of the mandarin fish is mixed with the cryopreservation diluent to realize cryopreservation; and the sperm of the mandarin fish is mixed with the ultra-cryopreservation anti-freezing solution to realize ultra-cryopreservation. The cryopreservation diluent for the sperm of the mandarin fish can realize cryopreservation of the sperm of the mandarin fish, and the sperm can be ultra-cryopreserved after the addition of the anti-freezing protective agent, so that the persistence of the preservation time of the sperm of the mandarin fish is ensured, the protection effect on the sperm is good, the activation rate of the sperm after thawing is high, and the sperm has good vitality.

The invention discloses an ultralow-temperature rice field carp semen preserving fluid and a method for preserving and recovering rice field carp semen, and belongs to the technical field of rice field carp artificial breeding. The ultralow-temperature rice field carp semen preserving fluid comprises a diluent and an anti-freezing agent, the components of the diluent are glucose, KCl and tris (hydroxymethyl) aminomethane, and the solvent is pure water; or the components of the diluent are trehalose, KCl and tris (hydroxymethyl) aminomethane, and the solvent is pure water; the antifreeze agent comprises one or two of methanol or ethylene glycol. According to the technology, an optimized diluent formula and a specific anti-freezing agent are combined, and a centrifugal washing step after unfreezing is combined, so that long-term and efficient preservation of the carp semen in the rice field in liquid nitrogen at the temperature of-196 DEG C is successfully realized, and the sperm motility, the mitochondrial function and the fertilization ability are remarkably improved after unfreezing.

The invention provides a metabonomics-based mongolian sheep semen liquid preservation diluent and preparation thereof, the diluent comprises a basic diluent, an antibiotic, a membrane stabilizer and a differential metabolite, the basic diluent comprises a buffer component, an organic acid, sugar and water, the buffer component is Tris, and the organic acid is citric acid; the sugar is selected from one or more of fructose, glucose, cane sugar and lactose; the basic diluent contains 2-3 g of a buffer component, 1-2 g of organic acid, 1-3 g of sugar and the balance of distilled water according to the volume of 100 mL. The invention further provides application of the preservation diluent in Mongolian sheep semen preservation. The technical bottleneck of 17 DEG C liquid preservation of the Mongolian sheep semen is broken through, and the preservation period of the sperms is prolonged by supplementing a large amount of micromolecular substances consumed in the preservation process of the sperms screened in metabonomics.

The utility model relates to a sperm freezing carrier and a sperm preservation device, the sperm freezing carrier comprises a slide glass body, the surface of the slide glass body is divided into a hydrophobic area and a screening area, the hydrophobic area surrounds the screening area, a hydrophobic layer is arranged on the hydrophobic area, and a hyaluronic acid layer is arranged in the screening area. By means of the hydrophobic effect of the hydrophobic layer on the hydrophobic area and the tension of the cryoprotectant, the cryoprotectant cannot leak out from the hydrophobic area, the cryoprotectant is effectively prevented from splashing out in the operation process, and the probability of sperm loss is reduced. The mature sperms can be adhered to the hyaluronic acid layer of the screening area, so that the recovery rate of the frozen sperms is improved, the purpose of screening high-quality sperms can be achieved, and meanwhile, the time for searching the sperms in the cryoprotectant liquid drops subsequently can be shortened.

The invention relates to the technical field of biological breeding, and discloses an application of a PDHB gene inhibitor in improving the preservation quality of frozen pig sperms.By means of the technology, exogenous miR-let-7a or mimics or analogues for inhibiting PDHB gene expression are added into frozen and preserved pig semen, so that the effects of improving the sperm motility and improving the semen quality are achieved; especially, indexes such as sperm motility, sperm mitochondrial ROS content, membrane potential and the like are obviously superior to those of all groups including a control group.

The present invention provides a strain of Bacillus velezensis TYF-QPY-F44 producing saccharifying enzyme and its application, belonging to the field of microbial technology. The classification name of the Bacillus velezensis TYF-QPY-F44 provided by the present invention is Bacillus velezensis, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, with a deposit number of CGMCC NO.30425, a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and a deposit date of April 24, 2024. The saccharifying enzyme activity of the Bacillus velezensis TYF-QPY-F44 provided by the present invention can reach 2001U / g, the saccharifying power can reach 649.52mg / gh, the liquefaction power can reach 1.49, and the prepared enhanced Daququ has a stronger aroma.

The invention relates to the technical field of biological breeding, and discloses an application of exogenous miR-92a or a simulant or an analogue in improving the preservation quality of fresh boar sperms.The exogenous miR-92a or the simulant or the analogue is added into the fresh boar sperm preserved at normal temperature through the technology, PI3K / AKT signal channels are activated by down-regulating expression of PTEN, and the preservation quality of the fresh boar sperm is improved. The effects of improving the sperm motility, improving the sperm plasma membrane intact rate and improving the semen quality can be achieved.

Anchovy sperm freezing preservation solution, and a preparation method and application method thereof, belong to the technical field of aquatic germplasm resource protection and breeding, and particularly relate to a method for collecting, preserving and recovering sperm of anchovy fish. The present application is to solve the problems of low efficiency, short preservation time and low sperm activity of the existing anchovy sperm collection, and the anchovy sperm freezing preservation solution is composed of methanol, lecithin, resveratrol and glucose, and is diluted with double distilled water to 1L. The application method is as follows: 1. sperm collection and quality evaluation; 2. preparation of anchovy sperm freezing preservation solution; 3. mixing the sperm with the pre-cooled 4 DEG C anchovy sperm freezing preservation solution, dispensing, and adopting a four-step method for programmed cooling; 4. oocyte collection; 5. sperm warming and activity detection; 6. artificial insemination and hatching. The sperm preservation time is prolonged from several hours to more than several years, and through rapid thawing, the sperm activity can reach more than 40%, and the fertilization rate can reach more than 57%.

The invention discloses a Utah halomonas having both aerobic denitrification and tetrahydropyrimidine production functions and a screening method and application thereof, relating to the field of environmental protection microbial technology. The strain was deposited in the General Microbiological Center (CGMCC) of the China Microbiological Culture Collection Administration on May 9, 2024, with a deposit number of CGMCC No. 30540; the strain EH-01 was isolated from a salt pond bottom mud sample of a salt field in Dalian City, and is a strain that uses small molecular organic matter to reduce nitrate to nitrate under aerobic conditions. The invention is a strain capable of reducing nitrite to nitrogen gas. When the carbon source is sodium acetate, the nitrogen source is sodium nitrate, the nitrogen source concentration is 200 mgN / L, C / N=2, the salinity is 60 g / L and the rotation speed is 90 rpm, the denitrification efficiency is as high as 97%. The strain can produce compatible solute ectoine while carrying out heterotrophic denitrification, and the maximum accumulation of ectoine is 118 mg / g. The strain can not only be applied to environmental protection fields such as treatment of high-salt nitrogen-containing wastewater from ships, but also has broad application prospects in biological preparation of ectoine.

The purpose of the present invention is to provide sperm having high motility. The present invention is a composition for sperm treatment containing exosomes. The composition for sperm treatment according to the present invention can be used as a sperm adjustment solution, a sperm dilution solution, a sperm preservation solution, an artificial insemination solution, an in vitro fertilization solution, a solution for improving sperm motility, or a solution for maintaining sperm fertilization ability.