187 results about "Sperm Preservation" patented technology

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to a plant lactobacillus B534 strain and application thereof in preparation of functional products beneficial to sleep improvement and emotion regulation. The lactobacillus plantarum B534 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum B534 is CGMCC No.33031. The lactobacillus plantarum B534 is named as lactobacillus plantarum B534. The plant lactobacillus B534 provided by the invention is a natural strain and has food safety; the lactobacillus plantarum B534 has relatively strong acetylcholin esterase inhibition activity, and also has relatively strong capability of synthesizing and secreting GABA (gamma-aminobutyric acid), 3-HB (3-hydroxybutyric acid) and 5-HT (5-hydroxytryptamine); meanwhile, the plant lactobacillus B534 has relatively strong acid resistance and cholate resistance and also has relatively strong tolerance to simulated gastrointestinal fluid, so that the plant lactobacillus B534 has relatively good intestinal colonization ability and has important application value in the fields of products beneficial to sleep improvement, emotion regulation products and the like.

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a strain of lactobacillus plantarum and an application of the lactobacillus plantarum in improvement of intestinal inflammation and repair of intestinal mucosa injury. The plant lactobacillus is preserved in the China General Microbiological Culture Collection Center (CGMCC for short) on August 1, 2024, the address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the plant lactobacillus is CGMCC No.31521. The strain has remarkable antioxidant, bacteriostatic and anti-inflammatory effects, can repair intestinal barrier injury of mice with colitis, promote renewal of intestinal stem cells, promote growth of intestinal beneficial bacteria and inhibit proliferation of harmful bacteria, can be widely applied to prevention and treatment of enteritis, and has a wide market prospect.

The invention relates to bacillus subtilis, a biocontrol inoculant prepared from the bacillus subtilis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as KC2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31372, and the preservation time is July 19, 2024. The bacillus KC2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus KC2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses a bovine rotavirus monoclonal antibody and a preparation method of a bovine rotavirus quantum dot immunochromatography test strip, and belongs to the technical field of bovine rotavirus monoclonal antibodies. According to the invention, a hybridoma cell strain BRV 3G9 is utilized, and a monoclonal antibody is prepared by adopting an ascites method; the BRV 3G9 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46138. The BRV 3G9 is named as BRV 3G9. According to the present invention, the concentrated live virus immunized mouse is adopted to prepare the monoclonal antibody with strong immunoreaction, and the BRV quantum dot immunochromatography test strip is researched and developed on the basis of the monoclonal antibody, and has advantages of convenient use, high efficiency, high sensitivity, strong specificity and the like, and can achieve the rapid clinical differential diagnosis of calf diarrhea pathogens, the problem that grassroots veterinarians lack a clinical rapid detection technology is solved, the diagnosis and treatment level and the prevention and treatment effect are improved, and technical guarantee is provided for efficiently preventing and treating calf diarrhea and guaranteeing calf health.

The present invention provides a Fusarium proliferatum and its application in oil degradation. Fusarium proliferatum was deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on November 20, 2023, with a deposit number of CGMCC No. 40988. The provided Fusarium proliferatum can effectively degrade oil.

The invention relates to pediococcus acidilactici XZ106, which is classified and named as pediococcus acidilactici, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.30094, the preservation date is March 22, 2024, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The pediococcus acidilactici XZ106 can (1) relieve insulin resistance, regulate insulin and glucagon secretion and improve liver gluconeogenesis, so that diabetes hyperglycemia is improved; (2) weight gain, fat accumulation and liver lipid accumulation of obese mice are inhibited, glycolipid metabolism disorder of the mice is improved, and the composition has excellent effects of reducing fat and relieving obesity; (3) the level of IL-10 can be remarkably improved, and the anti-inflammatory ability of mice is improved; (4) vascular injury is reduced, so that diabetic complications and various cardiovascular diseases of atherosclerosis are prevented.

The invention discloses a bacterial strain and a method for producing pyrroloquinoline quinone. The strain is pseudomonas putida N1-1 which is preserved in the China General Microbiological Culture Collection Center on January 2, 2025, the preservation number is CGMCC NO.33266, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing. The strain is separated from myxomycete. The bacterial strain is inoculated into a fermentation culture medium, standing fermentation culture is carried out for 7 days at room temperature under the dark condition, a fermentation product is obtained and filtered, fermentation filtrate containing pyrroloquinoline quinine is obtained, and the yield of pyrroloquinoline quinine reaches 12.21 mg / L.

The invention relates to the technical field of microorganisms, in particular to streptomyces noursei and application of the streptomyces noursei in preparation of polymycotin B. The invention provides streptomyces noursei GBC A-20 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the streptomyces noursei GBC A-20 is CGMCC No.27277. The strain can be used for efficiently fermenting and producing the multi-fungal element B by utilizing cheap raw materials, the yield of the multi-fungal element B is remarkably improved, and the chemical titer and the biological titer of the fermentation product nysfungin are greatly improved; the strain and the application thereof provide an economical and feasible solution for the industrial production of the multi-fungal element B and the nysfungin.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis EF-1 capable of reducing blood lipid, a microbial preparation and application of the enterococcus faecalis EF-1. The enterococcus faecalis EF-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 27, 2024, and the preservation number is CGMCC No.32097. The enterococcus faecalis EF-1 is named as enterococcus faecalis EF-1. The enterococcus faecalis EF-1 has the functions of: (1) relieving the weight increase speed of animals, reducing body weight gain, and preventing, relieving or treating obesity; (2) the contents of triglyceride, cholesterol and low-density lipoprotein cholesterol in liver and serum are reduced; (3) the liver function is protected, and the contents of ALT and AST in serum and liver are reduced; (4) liver lipid deposition and lesion are relieved; (5) relieving adipose tissue hypertrophy; (6) preventing, relieving or treating metabolic syndrome; (7) reducing blood sugar, improving insulin resistance and relieving diabetes mellitus; 8, increasing the content of short-chain fatty acid; and (9) regulating intestinal flora.

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.

The present invention discloses a heterotrophic nitrifying Brevundimonas DB‑4 and its application, belonging to the field of microbial technology. The heterotrophic nitrifying Brevundimonas (Brevundimonas) DB‑4 was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee on December 11, 2023, with a deposit number of CGMCC No.29279. The Brevundimonas DB‑4 provided by the present invention is derived from the decomposed matter of the mixed compost of bean cake and vegetable cypress, has a strong heterotrophic nitrification ability, can promote the conversion of ammonium nitrogen in the rhizosphere soil to nitrate nitrogen, and can effectively colonize in the soil, increase the nitrate nitrogen content in the soil and plants; at the same time, it has the ability to depotassium, thereby increasing the content of available potassium in the rhizosphere soil, can promote the improvement of crop quality, and can be widely used in the preparation of microbial fertilizers to improve the nitrogen conversion efficiency of fertilizers and reduce the application of chemical fertilizers.

The invention discloses an alpine stellate microzymes HZNK494 and an application of the alpine stellate microzymes HZNK494. The saccharomyces cerevisiae HZNK494 is named as the saccharomyces cerevisiae HZNK494, and is preserved in the general microbiological culture collection center of the China Committee for Culture Collection of Microorganisms on November 8, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.28902. The saccharomyces cerevisiae HZNK494 is named as saccharomyces cerevisiae HZNK494. The bacterial strain is obtained by screening and identifying blueberry fruits for the first time, and after the bacterial strain is applied to a fermentation process of special berries such as blueberries, raspberries, mulberries and strawberries and a single raw material or a composite raw material of medicinal and edible resources for biological deacidification, the bacterial strain is found to be capable of effectively reducing the acidity of the fruit wine; moreover, foreign impurities which are brought by other deacidification processes and influence the quality of the fruit wine are not introduced, and the high-quality functional fruit wine which is harmonious in fragrance, taste and mouth feel can be produced.

The invention discloses bacillus subtilis LXN-1, and the bacillus subtilis LXN-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC.NO 31697. The bacillus subtilis LXN-1 is a bacillus subtilis LXN-1 strain. The bacillus subtilis LXN-1 has an inhibition effect on various plant pathogenic bacteria, the inhibition rate on tomato botrytis cinerea can reach 61.5%, the inhibition rate on tobacco brown spot caused by alternaria longibrachiata can reach 72%, the bacillus subtilis LXN-1 also has a virus resistance effect, the inhibition rate on tobacco TMV viral plaque can reach 95%, and the bacillus subtilis LXN-1 has a good application prospect. In addition, the bacillus subtilis LXN-1 also has the potential of being compounded with pesticide bactericides with iprodione, trifloxystrobin, difenoconazole, pyraclostrobin, copper oxychloride and copper hydroxide as main components for use, and has market popularization and application values, and absorption of the plants to nutrient elements can be enhanced, and the growth of the plants can be promoted, and the bacillus subtilis LXN-1 also has the potential of being compounded with the pesticide bactericides with the iprodione, the trifloxystrobin, the difenoconazole, the pyraclostrobin, the copper oxychloride and the copper hydroxide as main components.

A method and application of Escherichia coli BL21star (DE3) BLBRTXST3 and its fermentation method for producing high-purity lactose-N-neotetraose, the invention relates to the technical field of human milk oligosaccharide production, Escherichia coli )BL21 star (DE3) BLBRTXST3 is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number CGMCC No. 32521 and the deposit date November 6, 2024. The address of the deposit institution is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Escherichia coli ( Escherichia coli )BL21 star (DE3) BLBRTXST3 is used in the fermentation production of lactose-N-neotetraose (LNnT). The present invention improves the yield of lactose-N-neotetraose, uses a chromatographic simulated moving bed to produce high-purity lactose-N-neotetraose with a purity greater than 94%, replaces the traditional solvent crystallization method, has simple separation and purification, is conducive to industrial production, and simultaneously produces linear lactose-N-neohexaose with a purity greater than 95%.

The invention discloses bacillus altitudinis JYD51 and application of the bacillus altitudinis JYD51 in aquaculture, the bacillus altitudinis JYD51 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32164, and the preservation date is October 10, 2024. The bacillus altitudinis JYD51 can be applied to aquaculture. The JYD51 strain disclosed by the invention can be fermented by using a culture medium rich in organic matters, and a fermented product of the JYD51 strain has no toxic effect on penaeus vannamei boone and crabs and has the effects of inhibiting infection of enterocytozoon hepatopenaei to the penaeus vannamei boone and inhibiting harm of microsporidia crabs to the crabs. Meanwhile, the microbial agent prepared by compounding the JYD51 strain and the lysinibacillus sphaericus JYQ7 can effectively and cooperatively control the harm of enterocytozoon hepatopenaei and microsporidia crabs, purify the water body environment for growth of the shrimps and the crabs, improve the survival rate of the shrimps and the crabs in aquaculture and improve the product quality.

The invention discloses lactobacillus paracasei and belongs to the technical field of microorganisms. The lactobacillus paracasei is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, China, the preservation number is CGMCC No.26029, and the preservation date is November 2, 2022. The lactobacillus paracasei has the advantages that the lactobacillus paracasei can be used for preparing the lactobacillus paracasei, and the lactobacillus paracasei can be used for preparing the lactobacillus paracasei; the lactobacillus paracasei capable of effectively relieving constipation and preventing aging through the antioxidant function of the lactobacillus paracasei is obtained for the first time, and the lactobacillus paracasei has the advantages of being convenient to eat, simple in treatment, high in relieving rate and free of toxic and side effects.

The invention discloses a bacterial strain for antagonizing multiple pathogenic fungi of potatoes and simultaneously promoting growth and application of the bacterial strain, and relates to the field of microbial agents, the bacterial strain is bacillus wiedmannii Bw34, the bacterial strain is preserved in China General Microbiological Culture Collection Center, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is November 11, 2024, and the preservation number is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The compound is used for preparing a fungicide for antagonizing potato pathogenic fungi and a fungicide for promoting potato growth. The Bw34 has the effect of comprehensively preventing and treating potato soil-borne diseases, meanwhile, potato growth and nutrient absorption are promoted, and the application prospect is good.

The present invention provides an animal Bifidobacterium lactis subsp. lactis for enhancing anti-tumor immunity of lung cancer and an application thereof. The present invention separates and preserves animal Bifidobacterium lactis subsp. lactis SDZL01 for enhancing anti-tumor immunity of lung cancer, and names it as animal Bifidobacterium lactis subsp. lactis SDZL01 strain. Animal Bifidobacterium lactis subsp. lactis has been deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on November 18, 2024, and its preservation number is Bifidobacterium lactis subsp. lactis CGMCC No. 32692. Animal Bifidobacterium lactis subsp. lactis or its metabolite indole‑3‑acetic acid can be used to regulate the immune microenvironment of NSCLC or enhance immunotherapy. Animal Bifidobacterium lactis subsp. lactis can enhance anti-tumor immunity and can be used to prepare products that enhance immunotherapy of lung cancer. Animal Bifidobacterium lactis subsp. lactis can improve the response rate of cancer immunotherapy population and expand the population of tumor patients who benefit from cancer immunotherapy.

The invention relates to the technical field of agricultural microorganisms, in particular to bacillus isothermal layer R6 for preventing and treating pine wood nematode disease and application thereof. The bacillus isothermal layer R6 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO. 32275. The strain has the activity of efficiently inhibiting and killing the pine wood nematodes, the fatality rate of fermentation liquor of the strain on the pine wood nematodes can reach 90% or above when OD600 is 0.5-2, and the disease index of pine tree seedlings infected with the pine wood nematode disease can be effectively reduced.

The invention discloses ester-producing saccharomycetes, which is classified and named as Pichia kudriavzevii SAU-NN5, and is preserved in the China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.32820, and the preservation time is November 27, 2024. The invention also discloses a screening method of the saccharomycetes, a strain fermentation characteristic and an aroma-producing characteristic in rice wine fermentation application. The pichia kudriavzevii SAU-NN5 obtained by the method disclosed by the invention has a huge application potential in the fermentation of the rice wine. The pichia kudriavzevii SAU-NN5 can be used for fermenting the rice wine.

The invention relates to the field of microorganisms, and discloses application of lactococcus lactis in a product for improving sleep. The lactococcus lactis is named as WHH2078, is separated from yoghurt butter collected from a country in Sankarst city in Tibet Autonomous Region in China, is preserved in China General Microbiological Culture Collection Center on February 25, 2021, has a preservation number of CGMCCNO.21831, and is classified and named as Lactococcus lactis. The Lactococcus lactis is named as Lactococcus lactis, and the Lactococcus lactis is named as Lactococcus lactis. The lactococcus lactis has the effects of shortening the sleep latency, prolonging the total sleep time, improving 5-hydroxytryptamine and gamma-aminobutyric acid of the brain, serum and colon, lowering the corticosterone level, regulating back the diversity of intestinal microorganisms alpha, regulating up the abundance of intestinal acidobacilli and lowering the abundance of actinomycetes, desulfurization bacilli and verruca microflora. The sleep disorder can be effectively improved.

The invention belongs to the technical field of bacteriophages, and discloses a proteus mirabilis bacteriophage PF05, a bacteriophage composition thereof and application of the proteus mirabilis bacteriophage PF05, the proteus mirabilis bacteriophage PF05 is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 22, 2024, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9687. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The bacteriophage is resistant to high temperature, wide in lysis spectrum, high in storage stability and good in gastric acid tolerance, can keep activity in the stomach for a long time, and has a good prevention and treatment effect on infection of proteus mirabilis in the intestines and the stomach. The compound can be prepared into medicines, environment disinfectants, feeds, water additives and the like for preventing and treating proteus mirabilis for application, not only effectively prevents and treats propagation of proteus mirabilis, but also has the effects of reducing the death rate of chicken flocks, reducing digestive tract infection of the chicken flocks, avoiding diarrhea of the chicken flocks and improving the resistance of the chicken flocks, and has a very good market application prospect.

The present invention provides a strain of Bacillus toyonensis that promotes callus of post-harvest potato tubers, which was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on May 6, 2024, with a deposit number of CGMCC No. 30494. The Bacillus toyonensis CGMCC No. 30494 strain of the present invention is separated from the callus potato tissue, has the advantages of fast reproduction, easy collection of strains, easy processing of post-harvest tubers, and accelerated generation speed of the tuber callus epidermal sealing layer (i.e., wound periderm, which is mainly composed of a cell layer of polyphenol suberin, polyester suberin and lignin), thereby reducing the weight loss rate of the tuber and the disease index of Fusarium infection.

The invention belongs to the technical field of biological medicines, and discloses a lactobacillus paracasei strain with an anti-hypoxia function, the name of the lactobacillus paracasei strain is 40509a, the classification name of the lactobacillus paracasei strain is lactobacillus paracasei, the preservation number of the lactobacillus paracasei strain is CGMCC No.31996, the preservation date of the lactobacillus paracasei strain is CGMCC No.31996, the preservation unit of the lactobacillus paracasei strain is China General Microbiological Culture Collection Center, and the preservation unit of the lactobacillus paracasei strain is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus paracasei 40509a can prolong the survival time of a CD-1 hypoxic mouse and inhibit the content of HIF1-alpha and PHD in serum of the hypoxic mouse so as to achieve the anti-hypoxic function, and can improve the activity of PC12 hypoxic cells and improve the expression of NSE and NGF of hypoxic cells so as to achieve the anti-hypoxic function.

The present invention discloses a strain of Pseudocanthomonas NJAU‑273, which is classified and named as Pseudocanthomonas Pseudoxanthomonas sp., deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with a deposit date of March 25, 2024 and a deposit number of CGMCC No.30114. The present invention discloses its application in straw composting. The present invention also discloses the microbial agent prepared by the present invention and the application of the microbial agent in straw composting. Compared with the treatment without inoculation of microbial agent, the treatment with inoculation of NJAU‑273 microbial agent effectively promotes the increase of the temperature of the compost pile, accelerates the composting process, improves the germination index of the compost product, and increases the total nutrients of the compost product.

The invention belongs to the technical field of biology, and discloses a strain of eurotium cristatum, the classification name of the eurotium cristatum is eurotium cristatum, and the preservation number of the eurotium cristatum is CGMCC (China General Microbiological Culture Collection Center) NO: 40145. The preservation date is March 10, 2022, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. After the strain is used for fermenting the Yingde black tea, a characteristic peak appears at the position of 2min on a chromatogram, the content of the characteristic peak is obviously higher than the peak area of chromatograms of black tea fermented by other strains, and the strain has potential obvious effects of reducing blood fat and relieving fatty liver after acting on the Yingde black tea. Meanwhile, the invention also provides application of the strain.

The invention provides lactobacillus coryniformis Z5-1 as well as a culture method and application thereof, and belongs to the technical field of food fermentation. The preservation number of the lactobacillus clavuliformis Z5-1 is CGMCC (China General Microbiological Culture Collection Center) No.31547, the lactobacillus clavuliformis Z5-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 5, 2024, and the lactobacillus clavuliformis Z5-1 has good degradation and conversion effects on ethanol. The lactobacillus clavuliformis Z5-1 is inoculated in a high-alcohol-degree or other fermentation environment, so that ethanol in the environment can be effectively degraded, or ethanol generated in the fermentation process is converted into various flavor substances, and the quality of fermented food is improved; and the strain culture mode is simple and convenient to operate, low in cost and easy to popularize and apply in laboratories and industrial production.

The present invention discloses Pseudomonas WH‑3 and its application, belonging to the field of microbial technology. The Pseudomonas WH‑3, Latin name Pseudomonas juntendi WH‑3, is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a deposit date of August 2, 2024, a deposit number of CGMCC No.31535, and a deposit location in Beijing, China. The Pseudomonas WH‑3 provided by the present invention is separated and screened from seawater contaminated by metolachlor. The strain is adapted to a wide range of salinities, can efficiently degrade metolachlor and 2‑methyl‑6‑ethylaniline, and has an efficient bioremediation effect on marine environments polluted by metolachlor and 2‑methyl‑6‑ethylaniline.

The invention relates to the technical field of microorganisms. The invention provides a Klebsiella oxytoca bacteriophage RDP-EC-23031 strain, and the Klebsiella oxytoca bacteriophage RDP-EC-23031 strain is preserved in the China General Microbiological Culture Collection Center on April 22, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.45963. The Klebsiella oxytoca bacteriophage RDP-EC-23031 strain has the advantages that the Klebsiella oxytoca bacteriophage RDP-EC-23031 strain can be used for preparing the Klebsiella oxytoca bacteriophage; the invention also provides a phage composition. The invention also provides application of the klebsiella oxytoca phage RDP-EC-23031 or the phage composition in preparation of a product for preventing and / or treating livestock and poultry diseases caused by klebsiella oxytoca and application of the klebsiella oxytoca phage RDP-EC-23031 or the phage composition in preparation of a product for killing and / or inhibiting klebsiella oxytoca. The invention also provides a bacteriophage pharmaceutical preparation, a drinking water additive, a feed additive, a disinfectant or a cleaning agent. The Klebsiella oxytoca bacteriophage RDP-EC-23031 provided by the invention is used for replacing antibiotics to be applied to prevention and treatment of livestock and poultry breeding, and solves the problems that diseases of livestock and poultry animals are acute and cannot be treated in time, and drug use is invalid due to drug resistance of pathogenic bacteria.