40 results about "Sperm Preservation" patented technology

A sperm drying liquid and a sperm preservation method, relating to the field of biological cell preservation, the sperm drying liquid can simulate the environmental state of the sperm growth state, can provide the basic needs of the sperm to survive in vitro and maintain a certain metabolic activity, and can better Protect the sperm and improve the integrity of the sperm. The sperm preservation method provided by the present invention adopts centrifugation to remove the semen in the semen, then mixes it with the above-mentioned sperm drying solution, and finally uses the evaporation drying method to dry. The whole process is simple to operate and does not require complex freezing and drying equipment. The treated sperm can be stored for a long time while still maintaining a high integrity. After long-term storage, it can be combined with egg cells to obtain a high cleavage rate and blastocyst development rate, especially suitable for field collection and collection of wild animal semen.

The invention belongs to the technical field of fish reproduction, and discloses a method for breeding Channaidae fish by using artificial insemination and hatching tanks. Selected broodstock are temporarily raised in a cement pond with a water temperature of 25-26°C, and induced labor is carried out; In a cement tank with a water temperature of 28-29°C, after the effect time is reached, check the egg squeeze every 2 hours, and perform a total of 3 times; after the egg squeeze, the broodstock is kept temporarily, and penicillin is used to prevent inflammation, and the female broodstock survives more than 75% ; The male fish takes the testis, and grinds it with a metal mesh in the sperm preservation solution; before fertilization, the sperm in the sperm preservation solution is activated with water, and then poured into the eggs for fertilization. The eggs are fertilized and left to stand for more than 2 hours, then hatched in the hatching bucket, and the hatching bucket is inflated; the fertilized eggs are hatched in the bucket for 2 days to produce membranes, and after another 1-2 days, the yolk sac is completely absorbed, and the seedlings are cultivated. The invention significantly improves the utilization rate of male broodstock and the postpartum survival rate of female broodstock, reduces site occupation and improves the success rate of seed hatching.

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

The invention discloses an ancherythroculter nigrocauda sperm preservation method. The ancherythroculter nigrocauda sperm preservation method comprises the steps that 1, a sperm preserving fluid is prepared: raw material components are weighed in proportion and are respectively dissolved by using distilled water and are mixed evenly, and the distilled water is added to achieve volume fixing; 2, sperm acquisition is performed: an adult ancherythroculter nigrocauda milter is toweled off, and urine and faeces are removed to avoid sperm pollution; the abdomen is pressed slightly, extruded-out sperm is collected by using a suction tube, and sunlight or ultraviolet irradiation is avoided in the acquisition process; 3, sperm storage is performed: acquired ancherythroculter nigrocauda sperm is arranged in a dry, clean and sealed container subjected to sterilization and disinfection, and the container containing the sperm and the preserving fluid is kept in a low-temperature light-avoiding mode after the acquisition is completed. By the adoption of the technical scheme, the in-vitro survival time of the ancherythroculter nigrocauda sperm can be effectively prolonged, the fertilization vitality of the sperm can be kept, and protective development and distant crossbreeding of ancherythroculter nigrocauda are achieved. The ancherythroculter nigrocauda sperm preservation method is low in cost, simple and short in process and easy to operate, enables the preserving time to be long and has important application value.