40 results about "Sperm Preservation" patented technology

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

The invention discloses a fish sperm preserving fluid which is prepared from 2.120-2.420g of NaCl, 1.602-2.202g of KCL, 0.178-0.478g of CaCl2, 0.133-0.333g of MgSO4.7H2O, 0.114-0.414g of NaHCO3, 0.128-0.328g of VC and 500mL of distilled water. The fish sperm preserving method comprises the following steps of: (a) sperm collection: wiping and drying the genital pore region of a mature milter by using a towel, slightly pressing the abdomen of the mature milter, and collecting the extruded sperm by using a suction tube; and (b) sperm preservation: putting the collected sperm into a clean container, adding the sperm preserving fluid of which the volume is 10-20 times of the volume of the sperm, and preserving in low-temperature light-tight places.

The invention discloses a preservation solution, an activation solution and a hatching method for Paramisgurnus dabryanus sperms, belonging to the technical field of aquaculture. The sperm preservation solution comprises the following components: 0.5% to 1.0% of trisodium citrate, 1.0% to 3.0% of glucose, 0.1% to 0.3% of sodium bicarbonate, 0.02% to 0.04% of potassium chloride and 0.01% to 0.02% of VC; the activation solution comprises the following components: 1 to 2% of glucose and 0.35% of sodium chloride; and the hatching method comprises the following steps: activating sperms preserved with the sperm preservation solution by using the activation solution, allowing the sperms to combine with eggs, placing fertilized eggs on a mesh, and carrying out hatching in static water. Compared with the prior art, the invention has the following advantages: through innovation of the activation manner of the Paramisgurnus dabryanus sperms in a specific sperm preservation solution, the maximum vitality of the sperms can be guaranteed; the fertilization rate and the hatching rate of fish eggs are improved; and the fry breeding production is guided.

The invention provides a safe and efficient animal semen disinfector and a preparation method therefor, which are aimed to fill the blank of animal semen disinfectors in the market. The safe and efficient animal semen disinfector includes the following components in percentage by weight: 4.0 to 6.0 percent of povidone iodine, 0.5 to 1.0 percent of antimicrobial peptides, 0.2 to 0.3 percent of vitamins, 0.1 to 0.2 percent of ethylenediamine tetraacetic acid, and distilled water. A mixture of the povidone iodine and the antimicrobial peptides is used as the semen disinfector for semen purification and disinfection for economic animals. Tests prove that the animal semen disinfector can safely kill the five commonest and most harmful viruses in boar semen, i.e., classical swine fever virus (CSFV), porcine circovirus type 2 (PCV-2), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV), as well as other most pathogenic microorganisms; meanwhile, the animal semen disinfector can prolong the sperm motility and the sperm preservation time to a certain extent, and is non-toxic and harmless to fertilized ovums and embryos.

The invention discloses a method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms. The method comprises the steps that siniperca scherzeri seminal fluid is taken and freeze-preserved with sperm preservation liquid and then is irradiated by ultraviolet light after being diluted, and sperms are subjected to genetic inactivation; the inactivated sperms and siniperca chuats eggs are subjected to mixed fertilization, and egg gynogenesis is started; the eggs are transferred into fresh water for cold shock treatment after being fertilized, genomes are doubled, and heterogenous gynogenesis diploid is generated; the siniperca chuats gynogenesis diploid is transferred into culture and incubation water, incubation is carried out at room temperature, management is carried out according to a common siniperca chuats fry culture method, and siniperca chuats gynogenesis diploid fry are obtained. The siniperca chuats offspring generated through inducing of the method can accelerate excellent gene homozygosis, excellent inheritable characters can be fast fixed, and the disease resisting capacity is remarkably enhanced. According to the method, the conditions are easy to obtain, full-female siniperca chuats groups with the growth speed about 20% higher than that of common male siniperca chuats can be obtained later, and high breeding potential and application value are achieved.

The invention discloses an ancherythroculter nigrocauda sperm preservation method. The ancherythroculter nigrocauda sperm preservation method comprises the steps that 1, a sperm preserving fluid is prepared: raw material components are weighed in proportion and are respectively dissolved by using distilled water and are mixed evenly, and the distilled water is added to achieve volume fixing; 2, sperm acquisition is performed: an adult ancherythroculter nigrocauda milter is toweled off, and urine and faeces are removed to avoid sperm pollution; the abdomen is pressed slightly, extruded-out sperm is collected by using a suction tube, and sunlight or ultraviolet irradiation is avoided in the acquisition process; 3, sperm storage is performed: acquired ancherythroculter nigrocauda sperm is arranged in a dry, clean and sealed container subjected to sterilization and disinfection, and the container containing the sperm and the preserving fluid is kept in a low-temperature light-avoiding mode after the acquisition is completed. By the adoption of the technical scheme, the in-vitro survival time of the ancherythroculter nigrocauda sperm can be effectively prolonged, the fertilization vitality of the sperm can be kept, and protective development and distant crossbreeding of ancherythroculter nigrocauda are achieved. The ancherythroculter nigrocauda sperm preservation method is low in cost, simple and short in process and easy to operate, enables the preserving time to be long and has important application value.

The invention belongs to the technical field of fish reproduction and discloses a method for reproducing snakehead fishes by using artificial insemination and a hatching barrel. The method comprises the following steps: temporarily rearing selected parent fishes in a cement pond at the water temperature of 25-26 DEG C and performing spawning induction; in the cement pond at the water temperature of 28-29 DEG C, after the effect time, carrying out inspection and ore squeezing once every 2 h, three times in total; temporarily rearing parent fishes after ore squeezing, preventing inflammation with a penicillin agent to keep the survival rate of female parent fishes at 75% or above; taking testes from male fishes, and grinding the testes in a sperm preservation solution in a separated manner with a metal net; and before fertilization, activating sperm in the sperm preservation solution, then pouring ores for fertilization, hatching the ores in the hatching barrel after leaving the ores tostand for more than 2 h, and inflating the hatching barrel; taking out fertilized ores after hatching the ores in the barrel for 2 days, and performing fry culture after yolk sacs are absorbed 1-2 days later. The method significantly the utilization rate of female parent fishes and the survival rate of the female parent fishes after spawning, reduces field occupation and increases the fry hatchingsuccess rate.

The invention provides a hybrid snakehead spermatozoa preserving fluid. Water is taken as solvent, and every one liter of preserving fluid comprises 7.5-8.5g of sodium chloride, 0.4-0.5g of potassium chloride, 0.1-0.15g of calcium chloride, 0.2-0.6g of sodium bicarbonate, 8-15g of glucose and the balance being water. The hybrid snakehead spermatozoa preserving fluid is simple in source, easy to operate and feasible, preservation is carried out at conventional low temperature, spermatozoa preserved with the preserving fluid are long in preservation life, high in activation rate and good in sperm motility, guarantee is provided for spermatozoa preservation and reproduction of hybrid snakehead, and the hybrid snakehead spermatozoa preserving fluid has good application prospect.

The invention provides a cryopreservation method of grouper sperms, and belongs to the technical field of sperm preservation. The cryopreservation method comprises raw materials in parts by weight asfollows: 32-52 parts of NaCl, 28-57 parts of KCl, 21-40 parts of CaCl2, 20-61 parts of trehalose, 18-44 parts of liquid nitrogen, 20-68 parts of an anti-freezing liquid and 26-50 parts of compound antibiotics. By combination with the cryopreservation method comprising steps of sperm collection, dilution, precooling and quick-freezing for low-temperature storage of a seminal fluid, the reactivatedsperms can keep vigor of fresh sperms by the aid of a reasonable cooling rate, and the cryopreservation method has great significance for establishment of a marine fish sperm bank and protection for germplasm resources and biodiversity.

The invention discloses a sperm cryopreservation method of scatophagus argus. The sperm cryopreservation method comprises four steps of sperm collection, sperm dilution, sperm cryopreservation and sperm thawing, firstly male scatophagus argus is selected to inject human chorionic gonadotropin into dorsal muscle, then the sperm of the scatophagus argus is collected by an artificial abdominal extrusion method, then the sperm and antifreezing liquid are mixed to obtain preservation liquid of the scatophagus argus sperm, a diluent is prepared by mixing any one of a TS-2 diluent, a TS-19 diluent, aHank's solution, a Cortland diluent and sterilized seawater with dimethylsulfoxide; the sperm preservation liquid is injected into a cryopreservation tube, then freezing is conducted by adopting a step-by-step cooling method, and then the cryopreservation tube is immersed in liquid nitrogen for long-term preservation; when taking for use, the cryopreservation tube containing the sperm preservation liquid is taken out from the liquid nitrogen, and then is placed in a water bath with the temperature of 42 DEG C. According to the sperm cryopreservation method, the sperm of the scatophagus arguscan be preserved for a long time, and the sperm survival rate and insemination rate after thawing and resuscitation are high.

The invention relates to an ultralow-temperature cryopreservation method for fenneropenaeus chinensis sperms, and belongs to the technical field of sperm preservation. The method specifically comprises the following steps: diluting collected fenneropenaeus chinensis semen with an ultralow-temperature freezing protection solution, performing programmed cooling to-180 DEG C, and transferring into liquid nitrogen for preservation. According to the method, the fenneropenaeus chinensis semen diluted by the ultralow-temperature freezing protection liquid is subjected to programmed cooling to-180 DEG C and then is transferred into liquid nitrogen for preservation, the sperm stops metabolic activity in an ultralow-temperature environment so as to prolong the service life. The ultralow-temperature freezing protection liquid is combined with water molecules in the solution to initiate the hydration action. The crystallization process of water is weakened to increase the viscosity of the solution so as to reduce the formation of ice crystals. Meanwhile, a certain molar concentration can be maintained inside and outside cells to reduce the concentration of electrolyte in an unfrozen solution inside and outside the cells, so that the cells are prevented from being damaged by solutes, and the cells are protected from being damaged by the solution and the ice crystals.

The invention provides a boar sperm preservative as well as a preparation method and application thereof, and belongs to the technical field of livestock breeding. The boar sperm preservative provided by the invention is prepared from glucose, sodium hydrogen carbonate, sodium citrate, EDTA, citric acid, trihydroxymethyl aminomethane, L-cysteine, levofloxacin, sodium carboxymethylcellulose, bovine serum albumin and Banqing toxin-removing oral liquid. Compared with a conventional diluent Zorlesco, the boar sperm preservative provided by the invention has a better preservation effect of boar sperms, can improve the antibacterial ability of boar semen, and prolongs the storage time of the boar sperms.

The invention discloses a low-temperature preservation diluent for semen of plectropomus leopardus, a preservation method and application, and belongs to the field of artificial breeding of plectropomus leopardus. The diluent comprises the following components in parts by weight: 3.5 parts of sodium chloride, 1.68 parts of sodium bicarbonate, 2 parts of potassium chloride, 2 parts of reduced glutathione, 0.44 part of glucose and 10 parts of fetal calf serum. And diluting the semen of the plectropomus leopardus and the diluent according to a dilution ratio of (1: 9)-(1: 99), and storing at 0-4 DEG C. According to the invention, a novel formula for preserving the semen of the plectropomus leopardus at the low temperature of 0-4 DEG C is confirmed and put forward for the first time, the formula has no toxic effect on sperms, the longest sperm preservation time can reach 72 hours, the fertilization rate and the hatching rate are still 71.54% and 61.84% after the sperms are preserved for 24 hours, and compared with fresh sperms, the sperm preservation time is effectively prolonged, the sperm motility rate is ensured, and the survival rate of the sperms is improved. The requirement of low-temperature short-term storage of sperms during artificial improved variety breeding of plectropomus leopardus can be met.

The invention discloses a procypris merus sperm cryopreservation method. The method comprises four steps of seminal fluid collection, seminal fluid dilution, sperm cryopreservation and sperm unfreezing, procypris merus seminal fluid is collected by adopting an artificial abdominal extrusion method, then the seminal fluid is mixed with a diluent, the mixture is mixed with antifreezing liquid, and sperm preservation liquid is obtained, and the diluent is any one of D-15 diluent, Hank's liquid and Ringer's Solution for fishes, wherein the antifreeze agent is any one of methanol, dimethyl sulfoxide and glycerol; the sperm preservation liquid is injected into a cryopreservation tube, and the cryopreservation tube immersed into liquid nitrogen for long-term preservation; when the sperm preservation liquid is taken, the cryopreservation tube filled with the sperm preservation liquid is taken out of the liquid nitrogen and then immediately placed in a water bath kettle at the temperature of 27DEG C or 37 DEG C or 47 DEG C to be unfrozen, and then obtained procypris merus sperms are used for artificial insemination. The method can preserve the procypris merus sperms for a long time, and the survival rate of the thawed and recovered sperms is high.

Provided is a cryopreservation container that prevents confusion of specimens to be used for treatment and that is suitable for individual management of fertilized eggs and the like to be cryopreserved. IC tags are attached to a cryopreservation tank 61, a canister 51, a cane 41, and cryopreservation containers such as an ovum storage container 1 and sperm storage containers 71, 81. The cryopreservation containers may also have individual identification codes attached thereto. An ovum storage container 11 is configured by fitting an IC tag 12 from the rear end of a rod-like part 2 of a conventional ovum storage container 1. In addition, the IC tag 12 is formed by providing an inlet 15 inside a thin-walled pipe 14 made of a synthetic resin. The inlet 15 may be fixed at both ends by means offixing members 18, 18 composed of a nonconductive material, or may be integrally molded with a nonconductive synthetic resin material 20.

The invention discloses a method for cryopreservation of sperms from anoplopoma fimbria. The method comprises steps of acquisition of sperms, preparation of a culture medium, a cryopreservation process of the sperms and resuscitation and activation processes of the sperms, namely acquiring sperms from anoplopoma fimbria at sexual maturity by using an artificial anesthesia method; according to thesperm osmotic pressure of the anoplopoma fimbria, preparing a sperm culture medium; mixing the acquired sperms and the culture medium according to a volume ratio of 1:2, and further adding dimethyl sulfoxide which accounts for 10% of the volume of the mixed liquid so as to obtain a cryopreservation mixed liquid; and putting a uniformly mixed sperm cryopreservation mixed liquid sample into a refrigerator of 4 DEG C for 15 minutes, allowing the sample to stand for 5 minutes at the opening of a liquid nitrogen bottle, moving the sample downwards, further allowing the sample to stand for 5 minutesabove the liquid level of liquid nitrogen, further allowing the sample to stand for 5 minutes on the liquid level of the liquid nitrogen, and immersing the sample into the liquid nitrogen, and performing cryopreservation. By adopting the method disclosed by the invention, the anoplopoma fimbria sperm preservation operation can be convenient, efficient and effective, the anoplopoma fimbria spermscan be prevented from rapid deactivation or death in vitro, the preservation quality of the sperms can be improved, the preservation time can be prolonged, and the anoplopoma fimbria sperms can be available at any time in actual production.