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Gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and preparation method

A technology of gentamicin sulfate and silk fibroin, which is applied in medical science, absorbent pads, bandages, etc., can solve the problems of silk fibroin having no antibacterial properties, reducing clinical treatment effect, and restricting the use of silk fibroin. Injury to human body, wide range of antibacterial, low cost effect

Inactive Publication Date: 2013-12-18
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the use of silk fibroin can promote the proliferation and differentiation of fibroblasts, and promote the healing of skin wounds, so silk fibroin can be used as a skin dressing, but since silk fibroin itself has no antibacterial properties, this is harmful to silk The use of protein is limited, therefore, the preparation of a silk fibroin scaffold with antibacterial ability has become an urgent problem to be solved
[0004] Gentamicin sulfate is a broad-spectrum antibiotic that is effective against both Gram-positive and Gram-negative bacteria. It is widely used in systemic or local infections caused by sensitive bacteria, but long-term use or excessive blood concentration in the body , will bring a series of toxic and side effects, such as ototoxicity, nephrotoxicity, hematuria, and even death after anaphylactic shock, which reduces the clinical treatment effect

Method used

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  • Gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and preparation method
  • Gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and preparation method
  • Gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Put 5 g of silkworm cocoons without chrysalis in 0.2wt% NaCO at 100°C 3 Boil in 100ml of aqueous solution for 2 hours, after degumming, wash silk fibroin with deionized water until neutral, dry in an oven at 60°C to obtain silk fibroin; take 2.5g of silk fibroin and add 25ml, 9.8mol / L lithium bromide aqueous solution After heating in a water bath at 60°C for 5 hours, it was dialyzed with deionized water (the molecular weight cut-off of the dialysis bag was 8000, the dialysis time was 3 days, and the water was changed once a day), and then centrifuged at 25°C and 10000r / min for 10min to obtain 3.5wt% silk protein solution;

[0042] (2) Add 0.1g Span80 into a flask containing 100ml of vegetable oil, stir at 60°C and 400r / min for 0.5h, then add 10ml of 10wt% gelatin aqueous solution, continue stirring for 180min, stir in ice bath for 15min, then add 25wt% Span80 Dialdehyde aqueous solution 0.1ml, continue to stir for 2h, add 40ml of acetone at 4°C, stir for 15min, sta...

Embodiment 2

[0048] (1) Boil 5 g of silkworm cocoons without chrysalis in 100 ml of 0.2wt% NaCO3 aqueous solution at 100°C for 2 hours, after degumming, wash the silk fibroin with deionized water until neutral, and dry it in an oven at 60°C to obtain silk fibroin; Add 1g of silk fibroin to 25ml, 9.8mol / L lithium bromide aqueous solution, heat in a water bath at 60°C for 3 hours, and then dialyze with deionized water (the molecular weight cut-off of the dialysis bag is 10,000, the dialysis time is 3 days, and the water is changed once a day), and then Centrifuge at 25°C and 10000r / min for 10min to obtain a 2wt% silk fibroin solution;

[0049] (2) Add 0.1g Span80 into a flask containing 100ml of vegetable oil, stir at 60°C and 400r / min for 2.5h, then add 10ml of 10wt% gelatin aqueous solution, continue to stir for 180min, then stir in ice bath for 15min, then add 25wt% pentadiene 0.1ml of aldehyde aqueous solution, continue to stir for 2h, add 40ml of acetone at 4°C, stir for 15min, stand st...

Embodiment 3

[0053] (1) Put 5 g of silkworm cocoons without chrysalis in 0.2wt% NaCO at 100°C 3 Boil in 100ml of aqueous solution for 2 hours, after degumming, wash silk fibroin with deionized water until neutral, dry in an oven at 60°C to obtain silk fibroin; take 7.5g of silk fibroin and add 25ml, 9.8mol / L lithium bromide aqueous solution After heating in a water bath at 60°C for 4 hours, dialyze with deionized water (the molecular weight cut-off of the dialysis bag is 12,000, the dialysis time is 3 days, and change the water once a day), and then centrifuge at 25°C and 10,000r / min for 10 minutes to obtain 10wt% silk fibroin protein solution;

[0054] (2) Add 0.1g Span80 into a flask containing 100ml of vegetable oil, stir at 60°C and 400r / min for 5h, then add 10ml of 10wt% gelatin aqueous solution, continue stirring for 180min, then stir in ice bath for 15min, then add 25wt% glutaraldehyde 0.1ml aqueous solution, continue to stir for 2h, add 40ml of acetone at 4°C, stir for 15min, stan...

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Abstract

The invention discloses a gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold and a preparation method, belonging to the technical field of high molecular materials. The preparation method comprises the following steps: dropwise adding gentamicin sulfate solution with concentration of 10-50 mg / ml into gelatin microspheres, placing overnight at the temperature of 4-25 DEG C and then performing freeze drying to obtain a gentamicin sulfate / gelatin microsphere complex; adding the gentamicin sulfate / gelatin microsphere complex into 2-10 percent by weight of silk fibroin solution, uniformly stirring and then performing freeze drying to obtain the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin porous composite scaffold, wherein the porosity of the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold is 75-95 percent, the aperture of the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold is 50-200 mum, and the gentamicin sulfate / gelatin microsphere complex-loaded silk fibroin scaffold has long-acting antibacterial and drug slow-release functions and can be applied to the fields of skin wound healing and wound dressing.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a silk fibroin scaffold loaded with gentamicin sulfate / gelatin microsphere complex and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, mainly divided into the epidermis, dermis and subcutaneous tissue, and has important barrier, mechanical, sensory and regulatory functions for the human body. But the skin is one of the most fragile and vulnerable organs of the human body. Skin ulceration, wounds, surgical injuries and burns are important causes of skin damage, and burns are the main cause of skin damage. According to statistics, the number of people who die from burns in the world is about 300,000 every year, while there are about 10 million burn patients in my country every year, of which 3.2 million need skin transplantation. Infection is an important factor restricting the healing of burn skin,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/44C08J9/28
Inventor 郭瑞张渊明薛巍蓝咏
Owner JINAN UNIVERSITY
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