Gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and preparation method

Abstract

The invention discloses a gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and a preparation method, belonging to the technical field of high molecular materials. The preparation method comprises the following steps: dropwise adding gentamicin sulfate solution with concentration of 10-50 mg/ml into gelatin microspheres, placing overnight at the temperature of 4-25 DEG C and then performing freeze drying to obtain a gentamicin sulfate/gelatin microsphere complex; adding the gentamicin sulfate/gelatin microsphere complex into 2-10 percent by weight of silk fibroin solution, uniformly stirring and then performing freeze drying to obtain the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin porous composite scaffold, wherein the porosity of the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold is 75-95 percent, the aperture of the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold is 50-200 mum, and the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold has long-acting antibacterial and drug slow-release functions and can be applied to the fields of skin wound healing and wound dressing.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a silk fibroin scaffold loaded with gentamicin sulfate / gelatin microsphere complex and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, mainly divided into the epidermis, dermis and subcutaneous tissue, and has important barrier, mechanical, sensory and regulatory functions for the human body. But the skin is one of the most fragile and vulnerable organs of the human body. Skin ulceration, wounds, surgical injuries and burns are important causes of skin damage, and burns are the main cause of skin damage. According to statistics, the number of people who die from burns in the world is about 300,000 every year, while there are about 10 million burn patients in my country every year, of which 3.2 million need skin transplantation. Infection is an important factor restricting the healing of burn skin,...

AI Technical Summary

Problems solved by technology

Studies have shown that the use of silk fibroin can promote the proliferation and differentiation of fibroblasts, and promote the healing of skin wounds, so silk fibroin can be used as a skin dressing, but since silk fibroin itself has no antibacterial properties, this is harmful to silk The use of protein is limited, therefore, the preparation of a silk fibroin scaffold with antibacterial ability has become an urgent problem to be solved

[0004] Gentamicin sulfate is a broad-spectrum antibiotic that is effective against both Gram-positive and Gram-negative bacteria. It is widely used in systemic or local infections caused by sensitive bacteria, but long-term use or excessive blood concentration in the body , will bring a series of toxic and side effects, such as ototoxicity, nephrotoxicity, hematuria, and even death after anaphylactic shock, which reduces the clinical treatment effect

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