Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof

A technology of amyloliquefaciens and powdery bacillus, which is applied to the direction of bacteria fixed on or in inorganic carriers, fixed on/in organic carriers, etc., which can solve the problem of high culture temperature, poor adaptability, and excellent strains in production Few other problems, to achieve the effect of wide antibacterial range, good stability, and broad application prospects of bio-control

Active Publication Date: 2018-06-12
JIANGSU GENGYUN CHEM CO LTD
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is aimed at the deficiencies of the prior art, especially the deficiencies such as fewer excellent bacterial strains in production, poor adaptability, and h

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof
  • Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof
  • Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0276] Example 1. A marine-derived Bacillus amyloliquefaciens GY30 strain, and its deposit number is: CGMCC NO.14480.

[0277] The spore-producing fermentation culture method of Bacillus amyloliquefaciens GY30 strain has the following steps:

[0278] (1) Inoculate the GY30 strain on the PDA medium for activation and culture, use an inoculating loop to pick the GY30 strain under aseptic conditions in an incubator equipped with seed liquid culture medium, shake culture at 28℃, 180r / min; adjust the bacteria Liquid concentration is 10 9 Cells / mL, as the first-level seed solution; the seed solution medium formula is: glucose 20g / L, potato 200g / L, water 1L;

[0279] (2) Inoculate the first-level seed liquid into a 50L fermentor equipped with 30L fermentation medium at an inoculum amount of 6%, and ferment and culture for 12-14h at 32°C and 180r / min to prepare the second-level seed liquid. Fermentation medium formula: rice bran 0.2%, peanut meal 1.5%, inorganic salt NaCl 0.025%, MnSO 4 0.0...

Example Embodiment

[0287] Example 2, the method for preparing GY30 powder by using the Bacillus amyloliquefaciens GY30 strain described in Example 1, and the steps are as follows: the Bacillus amyloliquefaciens GY30 strain is sporulated in a fermentor according to the method described in claim 2 Fermentation culture, the obtained fermentation broth is spray-dried with bentonite as a drying aid to obtain GY30 bacterial powder. The amount of bentonite is 7% of the fermentation broth, and the inlet air temperature of the spray tower is 160°C during spray drying.

[0288] The prepared GY30 bacterial powder can be used as an effective ingredient to prepare medicines or pharmaceutical compositions for preventing and treating rice sheath blight.

Example Embodiment

[0289] Example 3, the method for preparing GY30 powder by using the Bacillus amyloliquefaciens GY30 strain described in Example 1, and the steps are as follows: the Bacillus amyloliquefaciens GY30 strain is sporulated in a fermenter according to the method described in claim 2 Fermentation culture, the obtained fermentation broth is adsorbed and dried with rice husk powder as a carrier until the water content is less than 10%, and GY30 bacterial powder is obtained by passing through a 100-mesh sieve. The mass volume ratio of rice husk powder to fermentation broth is 1:1.

[0290] The prepared GY30 bacterial powder can be used as an effective ingredient to prepare rice microbial fertilizer.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an ocean-originated bacillus amyloliquefaciens GY30 strain. The preservation number of the ocean-originated bacillus amyloliquefaciens GY30 strain is CGMCC NO. 14480. The invention also discloses a spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain and a method for preparing bacterium power through the bacillus amyloliquefaciens GY30 strain. The bacillus amyloliquefaciens GY30 strain has the advantages of being high in salt tolerance, low in growth temperature, good in antibacterial effects, wide in antibacterial range and easy to culture, which do not exist in terrigenous microorganisms. The spore production fermentation medium composition and the culture method of the bacillus amyloliquefaciens GY30strain are simple, applied raw materials are mainly agricultural product leftovers, thereby being low in price, extensive in resources, high in strain growth speed, low in fermentation temperature, short in fermentation time, easy to control in fermentation conditions, high in spore fermentation yield and capable of reducing raw material costs and fermentation energy consumption and improving thefermentation production efficiency; bacterium powder prepared through the spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain is high ineffects on processing microorganism fertilizers or rice sheath blight disease preventing pesticides.

Description

technical field [0001] The invention relates to a bacterial strain derived from the sea, in particular to a Bacillus amyloliquefaciens GY30, and also relates to a fermentation and cultivation method of the Bacillus amyloliquefaciens GY30 strain, a method for preparing bacterial powder by using the bacterial strain and its use. Background technique [0002] Bacillus amyloliquefaciens belongs to the genus Bacillus and is a bacterium with high affinity with Bacillus subtilis. During its growth, it can produce a series of biologically active substances such as antibiotics, antibacterial proteins or polypeptides against animal and plant pathogenic bacteria and fungi. , viruses and nematodes have inhibitory effect, the bacterium is widely distributed in nature, easy to isolate and cultivate, grows fast, has good stability, is non-toxic and harmless to humans and animals, does not pollute the environment, has abundant metabolites, has broad-spectrum antibacterial activity and compar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N11/14C12N11/02A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N11/02C12N11/14C12N1/205C12R2001/07
Inventor 李楠贾杰张海军刘慕兰李惠陶忠蒋涛丁正庭徐全德
Owner JIANGSU GENGYUN CHEM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap