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Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof

A technology of amyloliquefaciens and powdery bacillus, which is applied to the direction of bacteria fixed on or in inorganic carriers, fixed on/in organic carriers, etc., which can solve the problem of high culture temperature, poor adaptability, and excellent strains in production Few other problems, to achieve the effect of wide antibacterial range, good stability, and broad application prospects of bio-control

Active Publication Date: 2018-06-12
JIANGSU GENGYUN CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is aimed at the deficiencies of the prior art, especially the deficiencies such as fewer excellent bacterial strains in production, poor adaptability, and higher cultivation temperature, and provides a salt-tolerant, low-cultivation temperature and simple nutrition derived from the ocean. Novel Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) GY30 strain CGMCC NO.14480

Method used

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  • Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof
  • Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof
  • Bacillus amyloliquefaciens GY30 and preparation and application of bacterium powder thereof

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Embodiment 1

[0276] Example 1, a strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) GY30 derived from the ocean, its preservation number is: CGMCC NO.14480.

[0277] The spore-producing fermentation culture method of the described bacillus amyloliquefaciens GY30 bacterial strain, its steps are as follows:

[0278] (1) Inoculate the GY30 strain on the PDA medium for activation culture, and pick the GY30 strain with an inoculation loop under sterile conditions in an incubator equipped with a seed liquid medium, 28°C, 180r / min shaking culture; adjust the bacteria Liquid concentration is 10 9 cells / mL, as a primary seed liquid; the formula of the seed liquid culture medium is: glucose 20g / L, potato 200g / L, water 1L;

[0279] (2) Inoculate the primary seed liquid into a 50L fermenter equipped with 30L fermentation medium according to the inoculum amount of 6%, ferment and cultivate at 32°C and 180r / min for 12-14h, and prepare the secondary seed liquid. The formula of the fermen...

Embodiment 2

[0287] Embodiment 2, utilize the bacillus amyloliquefaciens GY30 bacterial strain described in embodiment 1 to prepare the method for GY30 bacterium powder, its steps are as follows: the bacillus amyloliquefaciens GY30 bacterial strain is carried out producing spores by the method described in claim 2 in fermenter Fermenting and cultivating, the obtained fermented liquid is spray-dried with bentonite as a drying agent to obtain GY30 bacterial powder. The amount of bentonite is 7% of the fermentation broth, and the temperature of the air entering the spray tower is 160° C. during spray drying.

[0288] The prepared GY30 bacterium powder can be used as an active ingredient to prepare medicine or pharmaceutical composition for preventing and treating rice sheath blight.

Embodiment 3

[0289] Embodiment 3, utilize the method for preparing the GY30 bacterium powder of bacillus amyloliquefaciens GY30 bacterial strain described in embodiment 1, its steps are as follows: the bacillus amyloliquefaciens GY30 bacterial strain is carried out spore production by the method described in claim 2 in fermentor Fermenting and cultivating, the obtained fermented liquid is adsorbed and dried with rice husk powder as a carrier until the water content is lower than 10%, and passed through a 100-mesh sieve to obtain GY30 bacterial powder. The mass volume ratio of the rice husk powder to the fermented liquid is 1:1.

[0290] The prepared GY30 bacterial powder can be used as an active ingredient to prepare rice microbial fertilizers.

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Abstract

The invention discloses an ocean-originated bacillus amyloliquefaciens GY30 strain. The preservation number of the ocean-originated bacillus amyloliquefaciens GY30 strain is CGMCC NO. 14480. The invention also discloses a spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain and a method for preparing bacterium power through the bacillus amyloliquefaciens GY30 strain. The bacillus amyloliquefaciens GY30 strain has the advantages of being high in salt tolerance, low in growth temperature, good in antibacterial effects, wide in antibacterial range and easy to culture, which do not exist in terrigenous microorganisms. The spore production fermentation medium composition and the culture method of the bacillus amyloliquefaciens GY30strain are simple, applied raw materials are mainly agricultural product leftovers, thereby being low in price, extensive in resources, high in strain growth speed, low in fermentation temperature, short in fermentation time, easy to control in fermentation conditions, high in spore fermentation yield and capable of reducing raw material costs and fermentation energy consumption and improving thefermentation production efficiency; bacterium powder prepared through the spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain is high ineffects on processing microorganism fertilizers or rice sheath blight disease preventing pesticides.

Description

technical field [0001] The invention relates to a bacterial strain derived from the sea, in particular to a Bacillus amyloliquefaciens GY30, and also relates to a fermentation and cultivation method of the Bacillus amyloliquefaciens GY30 strain, a method for preparing bacterial powder by using the bacterial strain and its use. Background technique [0002] Bacillus amyloliquefaciens belongs to the genus Bacillus and is a bacterium with high affinity with Bacillus subtilis. During its growth, it can produce a series of biologically active substances such as antibiotics, antibacterial proteins or polypeptides against animal and plant pathogenic bacteria and fungi. , viruses and nematodes have inhibitory effect, the bacterium is widely distributed in nature, easy to isolate and cultivate, grows fast, has good stability, is non-toxic and harmless to humans and animals, does not pollute the environment, has abundant metabolites, has broad-spectrum antibacterial activity and compar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/02A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N11/02C12N11/14C12N1/205C12R2001/07
Inventor 李楠贾杰张海军刘慕兰李惠陶忠蒋涛丁正庭徐全德
Owner JIANGSU GENGYUN CHEM CO LTD
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