Co-culture supernatant containing autologous CIK cells and application thereof

A technology of cell culture and supernatant, applied in the direction of cell culture active agents, animal cells, tissue culture, etc., to achieve the effect of improving anti-tumor effect, enhancing immune function, and high biological activity
CN107779435AInactive Publication Date: 2018-03-09广州市金航生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州市金航生物科技有限公司
Publication Date
2018-03-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses co-culture supernatant containing autologous CIK cells and application thereof, wherein the co-culture supernatant containing the autologous CIK cells is prepared by a following method. The specific method comprises the steps that: (1) the autogenous CIK cells are cultured until the fourteenth day, and concentration of the autologous CIK cells reaches 1-5*10<6> cells / ml, and after centrifugation, supernatant 1 and bottom cells 1 are obtained respectively; (2) the bottom cells 1 are washed with normal saline and centrifuged to obtain bottom cells 2; (3) the supernatant 1obtained in step (1) is further centrifuged, and bottom flocculent is discarded to obtain supernatant 2; (4) the supernatant 2 of the step (3) and the bottom cells 2 of the step (2) are mixed, addedwith the normal saline, human albumin, and gentamicin sulfate to prepare cell suspension, namely, the co-culture supernatant containing autologous CIK cells. Natural cytokines synthesized and secretedby the autologous CIK cells are more effective in enhancing immune function, resolving myelosuppression and immunosuppression, and improving anti-tumor effects of the autologous CIK cells.
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Description

technical field

[0001] The invention belongs to the biological field, in particular to a co-culture supernatant containing autologous CIK cells and application thereof. Background technique

[0002] Advances in immune cell biology and immune molecular biology have advanced the application of immunology in tumor therapy. Nowadays, tumor immunotherapy has become one of the basic means of tumor treatment, among which cell-mediated adoptive immunotherapy is one of the current research hotspots. By infusing specific or non-specific immune effector cells that can inhibit the growth of tumor cells into tumor patients, it can directly kill tumor cells, or induce the body's immune response in patients to achieve the purpose of inhibiting and treating tumors. The current research and clinical preliminary data on tumor immune effector cells suggest that cytokine-induced killer (autologous CIK) cells are a new type of highly efficient MHC non-restricted immune effector with broad-spect...

Claims

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