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Corneal metaphase preserving liquid containing recombinant human serum albumin and preparation method thereof

A technology of human serum albumin and preservation solution, applied in the field of corneal mid-term preservation solution containing recombinant human serum albumin and its preparation, can solve problems such as exogenous pollution, improve the success rate, maintain collagen fiber structure and transparency , the effect of saving a long time

Inactive Publication Date: 2019-02-15
镇江雷音再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In response to the above problems, one of the purposes of the present invention is to provide a medium-term corneal preservation solution containing recombinant human serum albumin, to solve the technical problem of exogenous contamination caused by animal-derived serum

Method used

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  • Corneal metaphase preserving liquid containing recombinant human serum albumin and preparation method thereof
  • Corneal metaphase preserving liquid containing recombinant human serum albumin and preparation method thereof
  • Corneal metaphase preserving liquid containing recombinant human serum albumin and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A medium-term corneal preservation solution containing recombinant human serum albumin, containing glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal·HCl, riboflavin, thiamine·HCl , KCl, NaCl, NaH 2 PO 4 2H 2 O, glucose, dextran 40, sodium pyruvate, vitamin C, sodium chondroitin sulfate, sodium hyaluronate, non-animal source recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, 4-hydroxyethylpiperazine Ethylsulfonic acid, phenol red indicator, water for injection, arginine·HCl, cystine, histidine·HCl·H 2 O, isoleucine, leucine, lysine·HCl, methionine, phenylalanine, threonine, tryptophan, tyrosine, valine, of which water for injection is the solvent, other groups The concentrations of the points are: glutamine 0.6g / L, calcium pantothenate 0.002g / L, choline chloride 0.002g / L, folic acid 0.002g / L, inositol 0.004g / L, nicotinamide 0.001g / L, Pyridoxal·HCl 0.002g / L, riboflavin 0.0002g / L, thiamine·HCl 0.002g / ...

Embodiment 2

[0043] A medium-term corneal preservation solution containing recombinant human serum albumin, containing glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal·HCl, riboflavin, thiamine·HCl , KCl, NaCl, NaH 2 PO 4 2H 2 O, glucose, dextran 40, sodium pyruvate, vitamin C, sodium chondroitin sulfate, sodium hyaluronate, non-animal source recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, 4-hydroxyethylpiperazine Ethylsulfonic acid, phenol red indicator, water for injection, arginine·HCl, cystine, histidine·HCl·H 2 O, isoleucine, leucine, lysine·HCl, methionine, phenylalanine, threonine, tryptophan, tyrosine, valine, of which water for injection is the solvent, other groups The concentrations of the points are: glutamine 0.3g / L, calcium pantothenate 0.002g / L, choline chloride 0.002g / L, folic acid 0.002g / L, inositol 0.004g / L, nicotinamide 0.001g / L, Pyridoxal·HCl 0.002g / L, riboflavin 0.0002g / L, thiamine·HCl 0.002g / ...

Embodiment 3

[0056] A medium-term corneal preservation solution containing recombinant human serum albumin, containing glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal·HCl, riboflavin, thiamine·HCl , KCl, NaCl, NaH 2 PO 4 2H 2 O, glucose, dextran 40, sodium pyruvate, vitamin C, sodium chondroitin sulfate, sodium hyaluronate, non-animal source recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, 4-hydroxyethylpiperazine Ethylsulfonic acid, phenol red indicator, water for injection, arginine·HCl, cystine, histidine·HCl·H 2O, isoleucine, leucine, lysine·HCl, methionine, phenylalanine, threonine, tryptophan, tyrosine, valine, of which water for injection is the solvent, other groups The concentrations of the points are: glutamine 0.6g / L, calcium pantothenate 0.004g / L, choline chloride 0.004g / L, folic acid 0.004g / L, inositol 0.008g / L, nicotinamide 0.002g / L, Pyridoxal·HCl 0.004g / L, riboflavin 0.0004g / L, thiamine·HCl 0.004g / L...

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Abstract

The invention discloses corneal metaphase preserving liquid containing recombinant human serum albumin and a preparation method thereof. The corneal metaphase preserving liquid containing recombinanthuman serum albumin contains glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal.HCl, riboflavin, thiamine.HCl, KCl, NaCl, NaH2PO4.2H2O, glucose, dextran 40, sodium pyruvate, vitamin C, chondroitin sulphate sodium, sodium hyaluronate, non-animal recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, piperazine-1-erhanesulfonic acid, phenol red indicator, and water for injection. The non-animal recombinant human serum albumin is added to replace serum to improve corneal endothelial cell survival rate; and then the non-animal recombinant human serum albumin cooperates with a compound system of the chondroitin sulphate sodium, the sodium hyaluronate and the dextran 40, the survival rate of the corneal endothelial cell is greatly ensured.

Description

technical field [0001] The invention relates to the field of corneal medium-term preservation solution containing recombinant human serum albumin, in particular to a corneal medium-term preservation solution containing recombinant human serum albumin and a preparation method thereof. Background technique [0002] Human serum albumin (HSA) is the most abundant protein in human plasma, and its main functions include: (1) maintaining the colloid osmotic pressure and pH value of the solution; (2) important components such as lipids, metal ions, amino acids and other factors body binding and transport; (3) antioxidant function. [0003] The X-ray crystal structure of human serum albumin reveals that it is a heart-shaped molecule with 585 amino acids linked in a single chain, containing 67% α-helices. The remarkable feature of the structure of human serum albumin is that there are three homologous regions (I, II and III), each region is composed of two subdomains (A-B), each heli...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 苏萍童剑萍许伟吴佳宇肖丹
Owner 镇江雷音再生医学科技有限公司
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