3results about How to "Promote application development" patented technology

This invention relates to a quantitative method for ultrasonic disruption of *E. coli* cells, specifically a method based on firefly luciferase in *E. coli* cells. The method involves mixing *E. coli* expressing firefly luciferase as an internal standard with a suspension of target protein-expressing bacteria, followed by ultrasonic disruption of the cells. The degree of disruption of the target protein-expressing bacterial suspension is quantitatively calculated by measuring the activity of the firefly luciferase. The target protein-expressing bacterial suspension uses *E. coli* expressing the target protein. Compared with existing technologies, this invention overcomes the shortcomings of complex experiments, low efficiency, and unstable accuracy in existing technologies. It achieves efficient and highly accurate quantification of the degree of ultrasonic disruption of *E. coli* cells, reduces experimental complexity, and improves research and development efficiency and accuracy. This provides a scientific basis and new approach for the development of new feed protein resources and livestock breeding research, and has broad application potential.

This invention relates to a phthalate hydrolase mutant and its application in phthalate degradation, belonging to the field of microbial enzyme technology. The amino acid sequence of the phthalate hydrolase mutant HylD1 is shown in SEQ ID NO.1, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.2. This enzyme can catalyze the reaction to generate MMP using DMP as a substrate; or catalyze the reaction to generate MEP and EMP using DEP as a substrate. In this invention, the encoding gene of the phthalate hydrolase HylD1 from *Paracoccus koningii* BJQ0001 was artificially modified by site-directed mutagenesis at amino acid position 265, resulting in a mutant HylD1 (R265A) with higher activity. Compared with the wild-type phthalate hydrolase HylD1, the absolute enzyme activity of this phthalate hydrolase mutant HylD1 (R265A) in degrading DMP and DEP is increased by 2-fold and 3.6-fold, respectively, and a special product EMP is also produced during the degradation of DEP.

The application discloses a kind of magnetic film glass etchant and its preparation method and application, the chemical composition of glass etchant is: Li2O 0~2wt%, Na2O 1.5~2.5wt%, K2O 2~4wt%, MgO 2.5~4.5wt%, B2O38~12wt%, SiO210~20wt%, TiO21.5~5wt%, Al2O30.4~1.2wt%, Bi2O320~30wt%, V2O535~45wt%, Nb2O50.5~2.5wt%.The melting temperature of the glass etchant of the application is low, the expansion coefficient is low, the surface tension is small, the high-temperature viscosity is low, can penetrate to ceramic or glass surface through metal powder layer at high temperature, play the role of etching ceramic or glass surface, thereby increase the roughness and surface area of ceramic or glass surface, make metal layer and ceramic or glass bond firm, and will not increase the resistance of metal layer, effectively improve the heating efficiency, thereby be favorable to promote the technical progress and development of electromagnetic induction furnace magnetic heating material industry.