Preparation method for decreasing impurities of gentamicin sulfate

Abstract

The invention discloses a preparation method of gentamicin sulfate capable of reducing impurities, which can significantly reduce the impurities of gentamicin sulfate products, meets the requirements of "Chinese Pharmacopoeia" (2015 edition), and the production process is simple and easy to operate; The present invention differs from the prior art in the following three points: 0.001% adenosine triphosphate and 0.001% glycine are added to the spore slant culture medium; 0.001% adenosine triphosphate and 0.001% glycine are added to the seed culture medium; 0.003% adenosine triphosphate is added to the fermentation medium and 0.003% glycine; the total miscellaneous content of the gentamicin sulfate product produced before the improvement > 4%, and sisomicin > 1.5%; Somicin <1.5%, Minor Normycin (Small Normicin) <3%.

Description

technical field

[0001] The invention relates to the field of antibiotic fermentation, in particular to a preparation method of gentamicin sulfate capable of reducing impurities. Background technique

[0002] Gentamicin sulfate is an aminoglycoside antibiotic, which has good antibacterial effect on various Gram-negative bacteria and Gram-positive bacteria, and has good antibacterial effect on various Enterobacteriaceae bacteria such as Escherichia coli, Klebsiella, Proteus Genus, Salmonella, Shigella, Enterobacter, Serratia and Pseudomonas aeruginosa have good antibacterial effect, Neisseria and Haemophilus influenzae are moderately sensitive to this product. It also has certain effects on Brucella, Yersinia pestis, Acinetobacter, and Campylobacter fetus. It has good antibacterial effect on about 80% of methicillin-sensitive strains in Staphylococcus (including Staphylococcus aureus and coagulase-negative staphylococcus), but most of the methicillin-resistant strains are res...