Tea polyphenol detecting test paper, standard color comparison card and usage thereof

A standard colorimetric card and detection test paper technology, which is applied in biological testing, material inspection products, color/spectral characteristic measurement, etc., can solve the problems of high price, achieve long storage time, avoid the limitation of test conditions, and be convenient anytime and anywhere The effect of using

Inactive Publication Date: 2008-10-29
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Among the existing methods for the determination of tea polyphenols, some methods have high precision, and some methods are highly targeted, but expensive precision instruments are required; although titration methods do not require precision instruments, they require the preparation of reagents and the process of titration

Method used

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  • Tea polyphenol detecting test paper, standard color comparison card and usage thereof
  • Tea polyphenol detecting test paper, standard color comparison card and usage thereof
  • Tea polyphenol detecting test paper, standard color comparison card and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of test paper: Weigh 2.5g of soluble starch, dissolve in 80mL of water, heat to colorless and transparent, and prepare a 2.5% (W / V) starch solution, weigh 28.2g of potassium sodium tartrate, dissolve in the prepared starch solution , stir to dissolve, then add 27.0 g of ferric chloride, continue to stir and dissolve, and set the solution to 100 mL to obtain a color solution, in which the molar concentrations of potassium sodium tartrate and ferric chloride are both 1.0 mol / L. Soak the qualitative filter paper in the chromogenic solution for 10 minutes, take it out and place it on a glass plate and dry it in an oven at 37°C to obtain the test paper for the detection of tea polyphenols.

[0022] Preparation of standard color card: Tea polyphenols were used to prepare standard solutions with concentrations (mg / mL) of 0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0. Use the above-mentioned tea polyphenol detection test paper to develop the color of tea ...

Embodiment 2

[0030] 1. Preparation of test paper: Weigh 0.2g of agar powder, dissolve it in 80mL of water, heat to colorless and transparent, prepare a 0.2% (W / V) starch solution, weigh 28.2g of potassium sodium tartrate, dissolve in the prepared In the starch solution, stir to dissolve, then add 27.0 g of ferric chloride, continue stirring and dissolving until the solution reaches 100 mL, and obtain a color solution, in which the molar concentrations of potassium sodium tartrate and ferric chloride are both 1.0 mol / L. Soak the qualitative filter paper in the chromogenic solution for 5 minutes, take it out and put it on a glass plate in a cool and dry place to dry to obtain the tea polyphenol detection test paper.

[0031]2. Preparation of standard color card: Tea polyphenols were prepared into standard solutions with concentrations (mg / mL) of 0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0. Based on the color development of tea polyphenols with different standard concentrations b...

Embodiment 3

[0034] 1. Preparation of test paper: Weigh 2.0g of soluble starch, dissolve in 80mL of water, heat until colorless and transparent, prepare a 2% (W / V) starch solution, weigh 4.02g of sodium oxalate, dissolve in the prepared starch In the solution, stir to dissolve, then add 13.5g of ferric chloride, continue to stir and dissolve, and set the solution to 100mL to obtain a color solution, wherein the molar concentrations of sodium oxalate and ferric chloride are 0.3mol / L and 0.5mol respectively / L. Soak the qualitative filter paper in the chromogenic solution for 5 minutes, take it out and place it on a glass plate and dry it in an oven at 37°C to obtain the tea polyphenol detection test paper.

[0035] 2. Preparation of standard color comparison card: Based on the color development of tea polyphenols of different standard concentrations by the above-mentioned tea polyphenol detection test paper, the corresponding standard color comparison card was prepared, and the preparation ...

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Abstract

The invention provides tea polyphenol test paper, which is prepared by using transition metal, such as iron, as a color development agent; using tartaric acid, citric acid, oxalic acid, hydrofluoric acid and salicylsulfonic acid, or any of sylvite, sodium salt and potassium sodium tartrate thereof as a complexing agent; and using soluble starch, polyvinyl alcohol, agaragar, polyacrylamide gel or gelatin as a bonding agent. Meanwhile, a standard tea polyphenol concentration color card is prepared. The tea polyphenol content can be quickly and visually observed according to the contrast between the color development of a sample to be tested on the detecting test paper and the standard color card. The test paper is suitable for quickly measuring the tea polyphenol content in plants or plant products, particularly suitable for the measurement of tea polyphenol content in tea. The test paper has the advantages of simple method, convenience, rapidness, low cost and easy popularization.

Description

technical field [0001] The invention relates to the detection technology of plant active ingredients, and in particular belongs to a tea polyphenol detection test paper and a standard color comparison card thereof and their application. Background technique [0002] Tea is one of the three major natural beverages in the world. Tea polyphenols are important active components of tea and the main substances that determine the flavor and quality of tea. [0003] At present, the methods for determining the content of plant polyphenols mainly include high performance liquid chromatography, atomic absorption method, ultraviolet-visible spectrophotometry, titration method and electrochemical method. High-performance liquid chromatography determines that each component in the substance is separated and analyzed qualitatively and quantitatively. This method can simultaneously determine the fingerprints of different polyphenol components and multiple active ingredients. The atomic abs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/25G01N33/52
Inventor 赵艮贵田鹏史通麟董川
Owner SHANXI UNIV
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