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PLA2R (phospholipase A2 receptor) antibody detection strip and preparation method and detection method thereof

A PLA2R and antibody detection technology, which is applied in the direction of measuring devices, instruments, and disease diagnosis, can solve the problems of instability, inability to quantify the stability of colloidal gold, and easy molecular shedding, etc., achieving high stability, short detection time, and high sensitivity Effect

Inactive Publication Date: 2015-09-02
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the colloidal gold label is based on the principle of electrostatic adsorption, it is unstable in the fluid, and the labeled molecules are easy to fall off, and can only be read when the colloidal gold particles are enriched to a certain amount visible to the naked eye. Qualitative analysis of the detected object
At present, the rapid detection of phospholipase A2 receptor (PLA2R) antibody by colloidal gold immunochromatography is only mentioned in the publication number CN102159951A, but as mentioned above, it cannot be quantified and the stability of colloidal gold is poor

Method used

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  • PLA2R (phospholipase A2 receptor) antibody detection strip and preparation method and detection method thereof
  • PLA2R (phospholipase A2 receptor) antibody detection strip and preparation method and detection method thereof
  • PLA2R (phospholipase A2 receptor) antibody detection strip and preparation method and detection method thereof

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Effect test

Embodiment 1

[0034]A method for making a PLA2R antibody detection test strip, comprising the following steps:

[0035] a. Coupling PLA2R with quantum dots to obtain a quantum dot-PLA2R complex, and spraying it on the conjugate pad;

[0036] b. Coat PLA2R on the nitrocellulose membrane as a detection line, and coat PLA2R antibody on the nitrocellulose membrane as a quality control line. The distance between the detection line and the quality control line is 5 mm.

[0037] c. Lap the sample pad, the conjugate pad sprayed with the quantum dot-PLA2 complex, the nitrocellulose membrane with the detection line and the quality control line, and the absorbent paper on the sticky bottom plate in sequence, and each membrane material overlaps 1mm , After sticking, the test strips cut into a width of 4mm are PLA2R antibody detection test strips.

[0038] Method for preparing quantum dot-PLA2R complexes by coupling PLA2R to quantum dots:

[0039] Take 0.1ml of quantum dot (excitation wavelength 365nm...

Embodiment 2

[0068] A method for making a PLA2R antibody detection test strip, comprising the following steps:

[0069] a. Coupling PLA2R with quantum dots to obtain a quantum dot-PLA2R complex, and spraying it on the conjugate pad;

[0070] b. Coat PLA2R on nitrocellulose membrane as a detection line, and coat PLA2R antibody on nitrocellulose membrane as a quality control line, and the distance between the detection line and the quality control line is 3mm.

[0071] c. Lap the sample pad, the conjugate pad sprayed with the quantum dot-PLA2 complex, the nitrocellulose membrane with the detection line and the quality control line, and the absorbent paper on the sticky bottom plate in sequence, and each membrane material overlaps 1mm , After sticking, the test strips cut into a width of 4mm are PLA2R antibody detection test strips.

[0072] Method for preparing quantum dot-PLA2R complexes by coupling PLA2R to quantum dots:

[0073] Take 0.1ml of quantum dot (excitation wavelength 365nm, em...

Embodiment 3

[0101] A method for making a PLA2R antibody detection test strip, comprising the following steps:

[0102] a. Coupling PLA2R with quantum dots to obtain a quantum dot-PLA2R complex, and spraying it on the conjugate pad;

[0103] b. Coat PLA2R on nitrocellulose membrane as a detection line, and coat PLA2R antibody on nitrocellulose membrane as a quality control line, and the distance between the detection line and the quality control line is 10mm.

[0104] c. Lap the sample pad, the conjugate pad sprayed with the quantum dot-PLA2 complex, the nitrocellulose membrane with the detection line and the quality control line, and the absorbent paper on the sticky bottom plate in sequence, and each membrane material overlaps 1mm , After sticking, the test strips cut into a width of 4mm are PLA2R antibody detection test strips.

[0105] Method for preparing quantum dot-PLA2R complexes by coupling PLA2R to quantum dots:

[0106] Take 0.1ml from CdSe / ZnS, the surface group is -NH 2 The...

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Abstract

The invention relates to the biotechnology field and in particular relates to a PLA2R (phospholipase A2 receptor) antibody detection strip and a preparation method and a detection method of the PLA2R antibody detection strip. According to the PLA2R antibody detection strip, based on the double-antigen sandwiched antibody detection principle, PLA2R coats a nitrocellulose membrane, quantum dot coupled PLA2R is sprayed on a conjugate pad respectively, a sample pad, the conjugate pad, the nitrocellulose membrane and absorbent paper are sequentially lapped on a viscous PVC (polyvinyl chloride) bottom plate sequentially, the strip with certain width is cut out to form a detection card, the concentration value of the antibody is measured by a fluorescence analyzer, and the PLA2R antibody in the sample can be quantitatively detected; based on a quantum dot immunochromatographic method, the contents of PLA2R antibody in serum, plasma and whole blood can be quantitatively detected safely, accurately and rapidly in a noninvasive low-risk and low-cost manner, and the PLA2R antibody detection strip can provide assistance for preliminary screening of the idiopathic membranous nephropathy and illness monitoring.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a PLA2R antibody detection test strip and a production and detection method. Background technique [0002] According to the etiology, membranous nephropathy can be divided into idiopathic and secondary membranous nephropathy, and its diagnosis depends on clinical manifestations and nephrocentesis. Nephrocentesis is an invasive diagnostic method that does some harm to the patient. Foreign studies have found that about 75% of patients with idiopathic membranous nephropathy can detect anti-PLA2R antibodies through serological testing. Domestic literature also shows that the positive rate of detection in Chinese people is higher, reaching 82%, while secondary membranous nephropathy and The detection rate of patients with other types of glomerular diseases is very low, and the detection rate of healthy people is negative. Quantitative detection of serological PLA2R antibody can provide ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/564
CPCG01N33/564G01N33/577G01N2800/347
Inventor 张永顶马伟民马新民张大准
Owner SHENZHEN BLOT BIOTECH
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