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Use method of immunochromatographic kit for rapidly detecting novel coronavirus N protein

A coronavirus and immunochromatography technology, applied in immunoassays, measuring devices, analytical materials, etc., can solve problems such as insufficient to fully meet the needs of epidemic prevention and control, restrict the supply capacity of new coronavirus rapid detection services, and accelerate the epidemic. The effect of screening suspected cases on the front line, reducing social panic, and simple operation

Inactive Publication Date: 2020-07-10
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On January 26, the State Drug Administration approved 4 new coronavirus detection products from 4 companies including BGI, but the currently approved new coronavirus nucleic acid detection reagents are all based on nucleic acid detection methods, and a single sample uses nucleic acid fluorescence. The PCR method takes 3 hours to give the test results, and the nucleic acid sequencing method takes 6 hours to give the test results. More importantly, due to the extremely high requirements of the laboratory environment for gene amplification, the detection throughput is called the biggest constraint factor, which is This greatly restricts the supply capacity of rapid detection services for the new coronavirus, which is not enough to fully meet the needs of epidemic prevention and control

Method used

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  • Use method of immunochromatographic kit for rapidly detecting novel coronavirus N protein
  • Use method of immunochromatographic kit for rapidly detecting novel coronavirus N protein
  • Use method of immunochromatographic kit for rapidly detecting novel coronavirus N protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of novel coronavirus N protein immunochromatographic detection kit

[0039] 1) Use fluorescent microspheres to label another strain of mouse anti-new coronavirus N protein monoclonal antibody

[0040] Take 0.5mL fluorescent microspheres, add 1mg carbodiimide (EDC), 1mg N-hydroxysuccinimide (NHS), stir at room temperature, 120r / min for 3h, then add 100μL of another strain of mouse anti-new coronavirus For virus N protein monoclonal antibody, stir for 1 hour at room temperature at a speed of 120 r / min, then add 10 mg of BSA blocking solution, and continue stirring for 1 hour at a speed of 120 r / min. Centrifuge at 12000 r / min for 20 min at 2-8 °C, and remove the supernatant. Finally, the solid precipitate obtained after centrifugation was redissolved to 1 mL with 0.2 M phosphate buffer (pH=7.4), and then 1 μL of Proclin300 was added and stored at 4°C until use.

[0041] 2) Using fluorescent microspheres to label rabbit IgG polyclonal antibody

[0042] Tak...

Embodiment 2

[0071] Serum / Plasma / Whole Blood Sample Testing

[0072] The types of samples taken include serum / plasma / whole blood.

[0073] After the serum / plasma / whole blood sample is collected, no sample pretreatment is required, and the sample to be tested is directly added dropwise to the sample inlet of the test card and allowed to stand for 15 minutes, and then inserted into the fluorescence immunochromatography analyzer for detection, the instrument automatically calculates the sample T / C value, judge negative or positive by comparing with normal value range. In addition, when two fluorescent bands appear under ultraviolet light irradiation, it is positive. The clinical test results are shown in Table 5 below.

[0074] table 5

[0075]

[0076]

[0077] As can be seen from the clinical testing data in Table 5, all the 20 negative samples were detected as negative, which was consistent with the clinical diagnosis; all the 20 positive samples were detected as positive, and the...

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Abstract

The invention relates to a use method of an immunochromatographic kit for rapidly detecting novel coronavirus N protein. The immunochromatographic kit comprises a test strip, the test strip comprisesa detection line and a quality control line, the detection line is coated with an anti-novel coronavirus N protein antibody, and the quality control line is coated with a goat anti-rabbit polyclonal antibody. The immunochromatographic kit for rapidly detecting the novel coronavirus N protein provided by the invention can be used for detecting the novel coronavirus N protein in serum, plasma and whole blood samples so as to be used for diagnosing novel coronavirus pneumonia.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to a method for using an immunochromatography kit for rapidly detecting the N protein of novel coronavirus. Background technique [0002] The 2019 novel coronavirus (SARS-CoV-2) is a new strain of coronavirus that has never been found in humans before. Common signs of human infection with coronavirus include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. On January 26, the State Drug Administration approved 4 new coronavirus detection products from 4 companies including BGI, but the currently approved new coronavirus nucleic acid detection reagents are all based on nucleic acid detection methods, and a single sample uses nucleic acid fluorescence. The PCR method takes 3 hours to give the test results, and the nucleic acid sequencing method ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558
CPCG01N33/558G01N33/56983G01N33/577G01N2333/165G01N2469/10
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH
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