Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods for inactivating pathogens using broad-spectrum pulsed light

A pathogen and pulse technology, applied in the field of inactivating pathogens with broad-spectrum pulsed light, can solve the problems such as BPL not raised

Inactive Publication Date: 2002-04-10
PUREPULSE TECH
View PDF14 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While BPL has been shown to be useful for the inactivation of various microorganisms on solid surfaces and / or within certain relatively clear liquids, it has not been suggested that BPL can be used to remove bioderivatives containing proteins and / or other biomolecules of interest. contamination in the composition

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for inactivating pathogens using broad-spectrum pulsed light
  • Methods for inactivating pathogens using broad-spectrum pulsed light
  • Methods for inactivating pathogens using broad-spectrum pulsed light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Inactivation of SV40

[0059] African green monkey kidney (AGMK) cells were inoculated with simian virus 40 (SV40, ATCC VR-305). Virus was harvested when 75%-100% of cells exhibited cytopathic effect (CPE). The virus stocks were frozen and stored at -60°C in culture medium containing 10%-15% fetal bovine serum (FBS). SV40-containing samples were titrated with AGMK cells. The culture medium used to grow and maintain AGMK cells is Eagles minimal essential medium (E-MEM), containing 5%-10% heat-inactivated FBS, 10 mg / ml gentamicin, 100 units / ml penicillin, and 2.5 mg / ml amphotericin B.

[0060] Cell cultures were grown at 36-38°C in a humidified 5-7% CO2 atmosphere.

[0061] SV40 in E-MEM containing 10% heat-inactivated FBS was added in a volume of 0.2 ml to sterile polyethylene sample containers. Twelve samples were prepared. Nine samples were treated with BPL. Three samples were treated with one pulse of light, three samples were treated with two pulses...

Embodiment 2

[0065] Example 2: Inactivation of HIV-1

[0066] CCRF-CEM cells were inoculated with HIV-type I strain HTLVIIIB (Vanderbilt University, Nashville, TN). HIV-1 was collected when the infectivity reached a minimum of 80% as determined by immunofluorescence assay (IFA). Frozen virus stocks, stored at -60°C in culture medium containing 10%-15% FBS.

[0067] HIV-1 containing samples were titrated with MT-2 cells (NIH AIDS Research and Reference Reagent Program. Cat #237). The medium used for the growth and maintenance of MT-2 cells was supplemented with 2 mM L-glutamine, 25 mM Hepes, 2 g / L NaHCO 3 , RPMI 1640 (with phenol red) in 15% heat-inactivated FBS and 50 mg / ml gentamycin. at 36-38°C in humidified 5-7% CO 2 Cell cultures were grown in the atmosphere.

[0068] 0.2 ml of HIV formulated in RPMI containing 15% heat-inactivated FBS was added to a sterile polyethylene sample container. Twelve samples were prepared. Nine samples were treated with BPL. Among them, three copies...

Embodiment 3

[0073] Example 3: Inactivation of CPV

[0074] A-72 cells (ATCC CRL 1542) were inoculated with CPV (ATCCVR-2017). Virus was harvested when 75%-100% of the cells showed cytopathic effect (CPE). Frozen virus stocks, stored at -60°C in culture medium containing 10%-15% FBS.

[0075]CPV-containing samples were titrated with A-72 cells. The medium used for the growth and maintenance of A-72 cells was E supplemented with 5%-10% heat-inactivated FBS, 10 mg / ml gentamicin, 100 units / ml penicillin, and 2.5 mg / ml amphotericin B. -MEM. Humidified 5-7% CO at 36-38°C 2 Cell cultures were grown in the atmosphere.

[0076] 0.2 ml of CPV in E-MEM containing 5% heat-inactivated FBS was added to a sterile polyethylene sample container. Twelve samples were prepared. Nine samples were treated with BPL. Among them, three copies were treated with one light pulse, three copies were treated with two pulses, and three copies were treated with three pulses. Three untreated samples served as con...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method of reducing pathogen content in a biologically derived composition by irradiating it with at least one high-intensity short-duration pulse of broad-spectrum incoherent polychromatic light. The biomolecule of interest retains biological activity in the resulting treated composition. Biologically derived compositions, such as serum, plasma or other blood products, including insulin, transferrin, heparin, collagen, coagulation factor VIII and / or coagulation factor IX, or containing monoclonal antibodies, or genetically engineered cell lines, etc. The content of pathogens, such as viruses, bacteria, pyrogens, fungi and / or prions present in the resulting protein composition is reduced by irradiation with broad spectrum pulsed light. A single pulse of broad spectrum light significantly reduces the amount of HIV-1, SV40, canine parvovirus or bovine viral diarrhea virus in a biologically derived composition.

Description

Background of the invention [0001] The use of biologically derived compositions is ubiquitous in scientific research and in the manufacture of pharmaceuticals / therapeutics. Human blood is routinely used as a source of protein preparations for the treatment of various diseases and abnormalities; for example, fractions of plasma, providing factor VIII, factor IX, antithrombin, transferrin, albumin, immunoglobulins, and platelets , and other therapeutic agents. Tissue culture methods are commonly used in the production of many pharmaceutical / therapeutic agents and related compositions, such as recombinant DNA and / or recombinant proteins from genetically engineered cell lines, viral vectors, amino acids, peptones, insulin and monoclonal antibodies. Similarly, animal-derived reagents such as bovine serum albumin (BSA) and sheep blood fractions are routinely used in research and manufacturing. [0002] Since these compositions are derived from living organisms, including humans, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04A61K35/12A61K35/14A61K35/76A61K38/00A61K38/16A61K38/17A61K38/28A61K38/43A61K39/395A61L2/00A61L2/08
CPCA61L2/0011A61L2/0029A61L2202/11
Inventor H·W·科弗J·M·伯格尔C·J·麦克唐纳德S·马洛伊A·H·布什内尔J·R·库珀
Owner PUREPULSE TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com