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Method and kit for detecting various vitamins in serum/plasma at same time

A vitamin and kit technology, applied in the field of vitamin testing, can solve the problems of long experimental period, error in test results, poor repeatability of batch test results, etc., and achieve the effect of shortened test period and high precision and accuracy

Active Publication Date: 2017-05-10
GUANGZHOU FENGHUA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the detection of vitamins, the traditional method is the microbial method, which has high sensitivity and accurate results, but this method has many limitations: the entire experiment cycle is long, the repeatability of batch test results is poor, and the test results have large errors, and each time only The detection of a single vitamin type; and recently, the development of liquid phase tandem mass spectrometry has brought the detection of vitamins into a new field. popularized in the diagnosis of disease

Method used

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  • Method and kit for detecting various vitamins in serum/plasma at same time
  • Method and kit for detecting various vitamins in serum/plasma at same time
  • Method and kit for detecting various vitamins in serum/plasma at same time

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 Simultaneously detects the method for multivitamins in serum / plasma

[0032] 1. Main experimental steps:

[0033] (1) Prepare the internal standard working solution: use the isotopic internal standards and concentrations of various vitamins provided by the Cambridge Isotope Laboratory in the United States; calculate the amount of the internal standard working solution based on the amount of specimens in the current experiment. Mix the above-mentioned various internal standards to obtain a high-concentration internal standard mixture. When using, dilute the high-concentration internal standard mixture with a mobile phase at a ratio of 1:100, and pay attention to fully mixing to obtain an internal standard working solution.

[0034] (2) Analysis process:

[0035] 1. Centrifuge the fully coagulated whole blood or anticoagulated whole blood at 3000 rpm for 5 minutes to extract the upper serum or plasma;

[0036] 2. Add 100-500 microliters of serum / plasma to a...

Embodiment 2

[0076] Embodiment 2 simultaneously detects the kit of multivitamins in serum / plasma

[0077] According to different blood samples, the reagent ratio and tandem mass spectrometry conditions are respectively set. The kit implemented in this implementation can detect 11 water-soluble vitamins and 7 fat-soluble vitamins at one time. The vitamins that can be measured are shown in the following table:

[0078] water soluble vitamins fat soluble vitamins Niacinamide Vitamin A niacin Vitamin D2 Vitamin B1 Vitamin D3 Vitamin B2 25-Hydroxyvitamin D2 Pyridoxine hydrochloride 25-Hydroxyvitamin D3 Pyridoxamine Hydrochloride Vitamin E Pyridoxal hydrochloride Vitamin K1 folic acid pantothenic acid biotin Vitamin B12

[0079] The kit of this embodiment includes: internal standard mixed solution, deproteinized solution, mobile phase and probe washing solution, the internal standard mixed solution includ...

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Abstract

The invention discloses a method for detecting various vitamins in serum / plasma at the same time. The method includes following steps: (1), well mixing to-be-detected serum / plasma with deproteinized liquid, centrifuging, and taking supernate; (2), well mixing the supernate obtained in the step (1) with internal standard mixed liquid, adopting tandem mass spectrometry for detection, and comparing ion strength of vitamins and internal standards of the vitamins to acquire content of the vitamins in the to-be-detected serum / plasma. In addition, the invention further discloses a kit for detecting various vitamins in serum / plasma at the same time. The kit comprises the internal standard mixed liquid, the deproteinized liquid, a mobile phase and probe washing liquid. By the method, various water-soluble and fat-soluble vitamins can be extracted from the serum / plasma, and a tandem mass spectrometer is utilized to quantify more than ten vitamins in one specimen within 2-3min, so that detection period is remarkably shortened, and vitamin detection results are higher in degree of precision and accuracy.

Description

technical field [0001] The invention relates to vitamin detection technology, in particular to a method and kit for simultaneously detecting multiple vitamins in serum / plasma. Background technique [0002] Vitamins are a class of organic substances that humans and animals must obtain from food in order to maintain normal physiological functions, and play an important role in the growth and metabolism of the human body. The lack of vitamins in the body will cause metabolic disorders and various diseases, which are collectively referred to as vitamin deficiency. At present, vitamins can be divided into fat-soluble vitamins and water-soluble vitamins according to their solubility. Water-soluble vitamins include B vitamins, vitamin C, etc.; fat-soluble vitamins include vitamins A, D, E, and K. [0003] Among the fat-soluble vitamins, vitamin A is the main component of the photosensitive substance of the photoreceptor cells in the retina. Vitamin A deficiency can cause dry eye s...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 汪勤杨绪庆沈健李智强胡爱武
Owner GUANGZHOU FENGHUA BIOENG
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