124 results about "Ion strength" patented technology

A fluidic assay device or test format that can regulate or control the sample flow rate and modulate the manifestation of test results to reduce or eliminate errors is described. The assay device has a substrate with a flow-rate control zone that regulates the amount of time needed for development and appearance of a visual signal in the observation-feedback zone until the color transition in the detection zone reaches color stability. The present invention also describes absorbent articles incorporating such an assay device and methods of monitoring dehydration or testing ion strength of a urine sample using such a test format.

The invention relates to a cervical exfoliated cell preservative liquid, the preservative liquid comprises the following components with the contents (by weight): 20 percent to 50 percent of alcohols; 15 percent to 50 percent of anti-aggregation reagent; 5 percent to 10 percent of buffer solution; 1 percent to 20 percent of ion strength maintaining reagent; 0.01 percent to 0.5 percent of anti-microbial reagent; 0.1 to 5 percent of mucus dissolving reagent; and 0 to 0.5 percent of cleaning agent. Compared with the prior art, the preservative liquid can not only lead cells to maintain the shape in an in vitro liquid suspension environment, minimize the protein precipitation, dissolve larger protein substances, such as blood and mucus, and reduce the cell aggregation, but can also selectively eliminate or reduce red cells, effectively kill microbes, prevent the activity of reverse transcriptase and retain the integrity of nucleic acids and proteins for facilitating the later analysis; in addition, the preservative liquid can greatly reduce the costs of consumptive materials for the TCT detection, improve the sensitivity and the specificity of the cervical cancer screening and accelerate the promotion and the popularization of the TCT technology in a medical system.

The invention discloses liquid-base cell preservation liquid as well as a preparation method and application thereof, relating to the technical field of biology. The liquid-base cell preservation liquid contains the following components in parts by weight: 12-18 parts of a fixing agent, 2-8 parts of an anti-coagulation agent, 2-10 parts of a buffer agent, 0.5-0.8 part of an ion strength maintaining agent, 2-8 parts of an antimicrobial reagent, 0.05-0.2 part of a mucus dissolving agent and 0.1-0.4 part of a cleanser. The liquid-base cell preservation liquid has the advantages that the cell stability is high, cells are well fixed, the cost is low, the preserved cell forms are relatively complete, and the like. Besides, the invention further provides the preparation method of the liquid-basecell preservation liquid and application of the liquid-base cell preservation liquid in human body cast-off cells.

A tandem analysis system is provided for ionizing a substance, performing mass spectrometric analysis of various ion types generated, selecting and dissociating an ion type, the ion type having a specific mass-to-charge ratio, and thereby, repeating mass spectrometric analysis measurement on the ion of the ion type over n-th stages. A processing judges control content for the analysis next to MSn (the n-th stage mass spectrometric analysis) within a predetermined time, based on ion intensity being represented by an ion peak with respect to the mass-to-charge ratio of each ion in the MSn result. An ion detection unit judges isotope-peak from the measured ionized data. Assuming that the MS1 count number of a parent-ion peptide measured during a certain constant time-interval is I, a data processing unit makes the MS2 integration number-of-times or analysis time of the peptide proportional to 1 / I.

The invention discloses a tea leaf classifying method. The tea leaf classifying method comprises the following step of using a vapor phase-ion transfer joint technique to detect volatile organic compounds in different types of tea leaves, wherein detection information is used as classifying basis. The tea leaf classifying method is characterized in that feature information of different types of tea leaves are respectively represented by corresponding ion transfer time and ion strength under different retention time, the method comprises collection of a three-dimension spectrum map, selection of a spectrum map feature area, pre-processing of data and analysis of chemical metering data, so as to classify samples. The tea leaf classifying method has the advantages that the vapor phase-ion transfer joint technique can be widely applied to the field of quick analysis of qualities of foods and agricultural products containing complicated systems; the analysis time is short, the resolution is high, the sensitivity is high, the non-destructive detection can be performed, and the tea leaf classifying method can be applied to classify the types of tea leaves.

Internally calibrated pH and other analyte sensors based on redox agents provide more accurate results when the redox active reference agent is in a constant chemical environment, yet separated from the solution being analyzed in such a way as to maintain electrical contact with the sample. Room temperature ionic liquids (RTIL) can be used to achieve these results when used as a salt bridge between the reference material and the sample being analyzed. The RTIL provides the constant chemical environment and ionic strength for the redox active material (RAM) and provides an electrolytic layer that limits or eliminates direct chemical interaction with the sample. A broad range of RAMs can be employed in a variety of configurations in such “Analyte Insensitive Electrode” devices.

The invention discloses a preparation method of a self-repairing hydrogel type dressing material based on a layer-by-layer assembly function. The hydrogel dressing material is controllably constructed by selecting the optimal assembly elements, utilizing the advantages of a layer-by-layer assembly technology on constructing a gel membrane material, and adjusting the optimal assembly conditions (acid-base value, ion strength, solvent, etc.). Furthermore, based on the layer-by-layer assembly technology, target substances with different properties and performances can be assembled to the dressing material through many modes, and the target substances can be controllably released. The prepared dressing material has the following characteristics: (1) the dressing material has a strong dressing performance and excellent biocompatibility and thus is an ideal wet dressing material; (2) the dressing material has an excellent controlled release function and is capable of precisely releasing loaded drugs under specific incentive conditions; and (3) the dressing material has an excellent self-repairing performance and can repair damaged parts rapidly, the service life is prolonged, and the secondary damage caused by replacement is avoided.

A measurement EIC for quantitative ions and a measurement EIC for ions to be confirmed in the vicinity of the retention time of a target compound are displayed in an overlapping manner in a chromatogram display area. In addition, a standard center line corresponding to a standard value of the confirmation ion ratio, which expresses the ratio of the intensity of the confirmation ions to the intensity of the quantitative ions in the target compound and an upper limit line and a lower limit line demonstrating the permissible range of the intensity of the confirmation ions are displayed in an overlapping manner on the EIC. An analyst determines whether a peak used for identification originates from the target compound by determining whether the top of an EIC peak of the confirmation ions falls between the upper limit line and the lower limit line.

A mass spectrometer includes a flight control device for allowing ions from an ion source to repeatedly fly along an orbit in a flight space for predetermined times; a detecting device for detecting the ions after the ions repeatedly fly along the orbit for the predetermined times; and a data processing device for starting collection of ion strength data detected by the detecting device. The ion strength data is obtained during the flight of the ions along the orbit, or when the ions are headed toward the detecting device after departing from the orbit, or when one of the above situations is estimated.

The invention discloses a method for detecting various vitamins in serum / plasma at the same time. The method includes following steps: (1), well mixing to-be-detected serum / plasma with deproteinized liquid, centrifuging, and taking supernate; (2), well mixing the supernate obtained in the step (1) with internal standard mixed liquid, adopting tandem mass spectrometry for detection, and comparing ion strength of vitamins and internal standards of the vitamins to acquire content of the vitamins in the to-be-detected serum / plasma. In addition, the invention further discloses a kit for detecting various vitamins in serum / plasma at the same time. The kit comprises the internal standard mixed liquid, the deproteinized liquid, a mobile phase and probe washing liquid. By the method, various water-soluble and fat-soluble vitamins can be extracted from the serum / plasma, and a tandem mass spectrometer is utilized to quantify more than ten vitamins in one specimen within 2-3min, so that detection period is remarkably shortened, and vitamin detection results are higher in degree of precision and accuracy.

The invention provides an ion exchange membrane with the monovalent ion selectivity and a preparing method and application thereof. The ion exchange membrane with the monovalent ion selectivity comprises an ion exchange membrane body and a polyelectrolyte layer deposited on the surface of the ion exchange membrane body. The polyelectrolyte layer is composed by mixing conductive nanometer particles and polyelectrolyte. According to the ion exchange membrane with the monovalent ion selectivity and the preparing method and application thereof, a mixed polyelectrolyte solution is prepared by mixing the conductive nanometer particles and the polyelectrolyte, then the ion exchange membrane body is modified in a layer-by-layer self-assembly mode, and the ion exchange membrane with the monovalent ion selectivity is further prepared. The filming water absorption, the thickness, resistance and the separation selectivity of the ion exchange membrane with the monovalent ion selectivity can be controlled through the deposition layer number, the nanometer particle concentration and solution ion strength, the monovalent ion separation selectivity of the ion exchange membrane is further improved, and the quite-high practicability and broad application prospects are achieved.

The invention provides an improved method for ultra-filtrating and recycling protein in bean curd yellow slurry. In the invention, the bean curd waste water is pretreated by enzyme process before being ultra-filtrated, so that small molecule soybean lactalbumin is polymerized into polymer (homopolymer and heteropolymer), and the molecular weights of the polymer and small molecular oligosaccharide increases, then ultrafiltration membrane with big aperture and high flux is used to separate the polymerized lactalbumin. The enzyme treatment process does not need to be heated but just needs the afterheat of the bean curd yellow slurry. The natural pH and ion strength of the yellow slurry can be kept and do not need to be adjusted. The protein separating method has the advantages that the technique is simple, the operation is convenient, the protein recycle rate is high, the processing amount is large, membrane module is not easy to pollute and easy to put into industrialized production.

The invention discloses a method for measuring boric acid in a water-based adhesive for cigarettes, which comprises the following steps: accurately weighing a certain amount of water-based adhesive sample for cigarettes, putting the water-based adhesive sample for cigarettes in a microwave digestion tank, adding nitric acid (HNO3) and oxydol (H2O2) to perform pre-digestion, tightening to seal the digested water-based adhesive sample solution for cigarettes in the microwave digestion tank, and introducing the sample solution into an inductive coupling plasma emission spectrum mass spectrometer to carry out detection; and detecting characteristic ion strength of boron (B) element and on-line internal standard germanium (Ge) element in the sample solution, carrying out quantitative determination by an internal standard calibration curve method, calculating the content of B element in the water-based adhesive for cigarettes, and acquiring the content of HBO3 by using the reduction coefficient. The method disclosed by the invention has the advantages of high test accuracy, high sensitivity and favorable repetitiveness, and is simple to operate. The invention is suitable for technical personnel in tobacco industry and cigarette adhesive production enterprises to carry out quantitative determination on HBO3 in the water-based adhesive for cigarettes, and fills up the blank in the technical field.

The invention discloses a synthesis method of peroxide mimic enzyme nano-catalysis particles. The synthesis method comprises the following steps: performing a synthesis reaction on a solution containing a gold source and a palladium source in the presence of a stabilizing agent and a reducing agent to obtain nano gold-palladium alloy particles serving as the peroxide mimic enzyme nano-catalysis particles, wherein the molar ratio of the gold source to the palladium source is (1:5)-(5:1); the molar ratio of the reducing agent to the gold source and palladium source together is (5:1)-(1:20); the stabilizing agent is citrate; the reducing agent is citrate or NaBH4. The synthesis method disclosed by the invention is used for synthesizing stable nano gold-palladium alloy particles with surface plasmon resonance effect and peroxide mimic enzyme activity. The nano gold-palladium alloy particles have stable properties and obvious and stable surface plasmon resonance peak; the optimal pH for the enzyme activity is 3, and the requirement for ion strength in the reaction is not high; the synthesis method can be applied to the peroxide catalysis in a protein denaturation environment and has excellent application potential in the fields of analysis and molecular computation.

The invention relates to a method for settling, filtering and removing carbon nanotubes by using calcium ions, is suitable for treating a water body polluted by the carbon nanotubes and belongs to the field of nanotechnology and environmental protection technology. The method comprises a step of: agglomerating carbon nanotubes by adding calcium chloride into carbon nanotube solution so as to remove the carbon nanotubes in the water body by a filtering method. When the calcium ions have a concentration of 0.001mol / L, the clearance rate of the carbon nanotubes is higher than 99 percent; a residual amount is less than 5 mu g / ml; and water solution is colourless and transparent. Under the conditions of different acid and alkaline degrees and ion strengths, the method also can effectively eliminate the carbon nanotubes. Under the presence of a surface active agent and a natural organic matter, the same excellent elimination effect also can be achieved by improving the additive amount of the calcium chloride moderately. The concentration of the calcium ions used for elimination is lower, and purified water is moderately hard water and can be discharged directly without softening treatment.

The invention discloses a method for preparing photoelectric material CsPbBr3. The method includes the steps that an HBr water solution with Pb2+ and an HBr water solution with Cs+ are mixed at the certain temperature, and after a reaction is completed, reacting products are washed and subjected to vacuum drying, and CsPbBr3 polycrystal powder is finally obtained. According to the method, the ion strength of the solution is adjusted by controlling the molar ratio of Cs+ / Pb2+, the problem that CsPbBr3 is instable in the water environment is solved, and therefore a single-phase CsPbBr3 material can be prepared in the water solution. The method is simple in preparing technology, low in used raw-material cost and suitable for mass production.

The invention relates to a method for preparing PLGA microspheres with hydrophilic nano-cellulose as an emulsifier. After the nano-cellulose is adsorbed to the surfaces of the PLGA microspheres, the nano-cellulose stabilizes an oil-water interface as a stabilizer. Meanwhile, because the nano-cellulose has certain zeta electric potentials, the nano-cellulose is easy to scatter and difficult to gather, after an oil phrase and a water phrase are mixed, there is no need to consume a large amount of energy, and the microspheres can be formed through simple ultrasound. Finally, due to the presence of the zeta electric potentials, in the formation process of the microspheres, the thickness of electrified layers of the surfaces of the microspheres can be further adjusted by adjusting the ion strength in the water phase, such as by adding sodium chloride, and the function of adjusting the particle size of the microspheres is achieved at last.

The invention discloses an ultrafiltration membrane separation method for obtaining royal jelly major protein and active filtrate in royal jelly. The method comprises the following steps: weighing royal jelly, stirring and mixing uniformly with water at the temperature of 4 DEG C, regulating pH by using NaOH, regulating the ion strength by using NaCl, and removing particles by using a 0.2mu m microfiltration membrane; separating and removing macromolecular impurities by using an ultrafiltration membrane M1 with the molecular weight cutoff of 100kDa, separating and concentrating the royal jelly major protein in the M1 filtrate by using an ultrafiltration membrane M2 with the molecular weight cutoff of 49kDa, supplementing purified water when the royal jelly major protein is concentrated to be 1 / 3 volume, allowing a small molecule solution to pass through the M2, wherein the obtained cutoff solution is a purified and concentrated royal jelly major protein solution, and performing freeze drying to form the royal jelly major protein freeze-dried powder. The small molecule solution contains polysaccharides and 10-hydroxy-2-caproleic acid (10-HDA) and can be used for production of functional drinks. The royal jelly major proteins (MRJPs) are separated and concentrated by using an ultrafiltration device, the extraction rate of the MRJPs is 82.63 percent, and the royal jelly major protein is subjected to freeze-drying to form the freeze-dried powder with the protein content of more than 70 percent. Moreover, the filtrate is used for preparing drinks, and comprehensive utilization of the royal jelly is realized.

The invention discloses an intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology and a preparation method of the intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology. The intelligent hydrogel, thermo-sensitive hydroxybutyl chitosan, which is of a single system and has high responsiveness to temperature and ion strength is adopted, the feature that sol-gel phase change is caused by the temperature and the ion strength of the thermo-sensitive hydroxybutyl chitosan is utilized, and the formed hydrogel stent is obtained through adjustment and control over the temperature and 3D printing parameters; then, through inorganic-salt-induced phase change, the hydrogel stent is evenly shrunk, and the mechanical strength and the precision of the hydrogel stent are further improved. The intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology overcomes the defects that 3D printing hydrogel is generally poor in formability, low in mechanical strength, low in precision and the like. Compared with existing common methods of adopting photosensitizer, complex-system hydrogel and the like, the intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology does not need introducing of any photosensitizer or cross-linking agent, is simple in system and gentle in technology; and the prepared 3D stent has high mechanical strength and is provided with a precise internal structure, and the prepared 3D stent can be widely applied to tissue engineering and tissue repair of the cartilage, the skin, the vessel, the nerve, the myocardium and the like.

The invention relates to fluorescent nano-particles. The fluorescent nano-particles comprise quantum dots and high-molecular copolymers coating outside of the quantum dots. A novel nano-probe prepared from the fluorescent nano-particles has the characteristics of temperature sensitivity and independent luminance from pH and ion strength, and the existing problem for detecting the temperature in cells can be solved.

The invention discloses linseed oil emulsion taking compounded linseed gum as an emulsifier and a preparation method of the linseed oil emulsion. The preparation method of the linseed oil emulsion comprises the following steps: 1) preparing a water solution of the emulsifier as a water phase, wherein the emulsifier is a mixture of Arabic gum and the linseed gum; 2) taking linseed oil as an oil phase and adding the oil phase into the water phase to obtain a primary emulsion; 3) homogenizing the primary emulsion to obtain the linseed oil emulsion. The linseed oil emulsion disclosed by the invention is small in grain diameter, good in dispersity and high in physical stability; meanwhile, the linseed oil emulsion has relatively high oxidization stability under different temperatures and pH (Potential of Hydrogen) conditions, and has a stronger environment change resisting capability when being compared with emulsion prepared by protein-polysaccharide layer-by-layer preparation; the linseed oil emulsion can keep relatively good stability under the conditions of relatively wide pH range and relatively great ion strength and in process treatment of sterilization, freeze thawing and the like. The linseed oil emulsion prepared by the invention has good storage stability; compared with a pure oil system of the linseed oil emulsion, the linseed oil emulsion has relatively good protection effect on linolenic acid.

The invention discloses a classifying method of vegetable oils. The classifying method adopts a gas phase-ion migration combination technology to detect volatile organic compounds in different types of vegetable oils, and the information is used as a classifying foundation. The classifying method adopts ion migration time and ion strength corresponding to different retention times to type characteristic information of each different vegetable oil, and comprises the following steps of collecting a three-dimensional spectral graph; selecting the characteristic area of the spectral graph; preprocessing data; and performing stoichiometric data analysis, so that a sample is classified. According to the classifying method disclosed by the invention, the gas phase-ion migration combination technology can be widely applied in the field of quick analyzing of quality of each food and each agriculture product containing a complex system, the analyzing time is short, the resolution rate is high, the sensitivity is high, and non-destructive detection is adopted, so that the classifying method can be applied for the classifying of the vegetable oils.

The invention belongs to the technical field of radioactive substance processing methods, and particularly relates to a method for analyzing fluorine ion concentration under a radioactive condition. The method is characterized in that a fluorine ion selective electrode method is adopted, by using sulfosalicylic acid-trisodium citrate as a total ion strength regulation buffer solution, the calculation is carried out by using a secondary standard addition method under a condition that pH is 8-9 so as to obtain F-concentration in an original sample. Aiming at the sample containing BF<4><->, the method provided by the invention has the advantages that complexing action on the ion F by the ion Al is skillfully utilized to ensure that BF<4><-> is completely hydrolyzed; in an analyzing process, distillation operation is avoided, and the loss of radioactive substances is reduced; moreover, a value of a slope S is not needed, and therefore, the errors caused by improper setting of the slope S can be avoided.

Internally calibrated pH and other analyte sensors based on redox agents provide more accurate results when the redox active reference agent is in a constant chemical environment, yet separated from the solution being analyzed in such a way as to maintain electrical contact with the sample. Room temperature ionic liquids (RTIL) can be used to achieve these results when used as a salt bridge between the reference material and the sample being analyzed. The RTIL provides the constant chemical environment and ionic strength for the redox active material (RAM) and provides an electrolytic layer that limits or eliminates direct chemical interaction with the sample. A broad range of RAMs can be employed in a variety of configurations in such “Analyte Insensitive Electrode” devices.

The invention relates to a preparation method of L-tyrosine, belonging to the field of biotechnology. The preparation method of L-tyrosine comprises the following steps: inoculating a culture medium with brevibacterium and performing fermentation culture to obtain fermentation liquid; performing centrifugation and ultrafiltration of the fermentation liquid to obtain ultrafiltration liquid; adjusting the pH value and ion strength of the ultrafiltration liquid; adding a surfactant and the ultrafiltration liquid into a supercritical extractor, wherein the surfactant and supercritical fluid form a supercritical fluid reverse micelles system for supercritical extraction of L-tyrosine; adding the back-extraction water-phase solution and extraction product into the supercritical extractor for supercritical back extraction; and after the back extraction, fetching the water phase and performing nanofiltration, crystallization and freeze drying to obtain purified L-tyrosine. The separation and purification method provided by the invention has high efficiency in extraction separation and extraction, and the obtained L-tyrosine has high activity and purity as well as good water solubility.

A method of mass spectrometry is disclosed comprising setting an attenuation factor of an attenuation device to a first value and then separating or filtering ions according to a first physico-chemical property and separating or filtering ions according to a second physico-chemical property and obtaining a multi-dimensional array of data. The most intense ion peak within one or more subsets of the multi-dimensional array of data is determined. If it is determined that the most intense ion peak would cause saturation of an ion detector or ion detection system then the method further comprises adjusting the attenuation factor of the attenuation device to a second value and obtaining mass spectral data wherein the adjustment of the attenuation factor substantially alters the intensity of all ions which are detected by the ion detector or ion detection system equally and irrespective of the mass to charge ratio of the ions. The intensity of the mass spectral data is then scaled based upon the degree to which the attenuation factor of the attenuation device was increased or reduced.

The invention relates to a method for reducing the content of protein in sodium hyaluronate prepared by a bio-extraction method. At present, a comb extract method for producing the sodium hyaluronate usually comprises the following steps: coarse cleaning, fine cleaning and grinding, enzymolysis, primary adsorption, CPC precipitation and dissociation, secondary adsorption, alcohol precipitation, and drying to obtain sodium hyaluronate crude drug. According to the method, after performing the CPC precipitation and dissociation, purified water is added to a dissociation solution, so that the ion strength of the dissociation solution is reduced; based on the quaternary ammonium salt complex-precipitation dissociation principle, positive ions on a quaternary ammonium group is free under the low salt concentration and is combined with negative ions on a mucopolysaccharide molecule to form a quaternary ammonium complex, and the quaternary ammonium complex is gradually dissociated under the high salt concentration. By virtue of the reversible reaction, precipitates in the dissociation solution are repeatedly complexed, and then are subjected to the steps of rinsing, secondary dissociation, filtering, alcohol precipitation and drying so as to obtain the sodium hyaluronate crude drug. According to the method, the content of protein in the sodium hyaluronate can be remarkably reduced.

The present invention provides a material for preparing a protective layer for a PDP, which reduces discharge delay time, improves temperature dependency, and has enhanced ion strength; a method of preparing the same; a protective layer formed of the material; and a PDP including the protective layer. More particularly, a material for a protective layer that includes monocrystalline magnesium oxide doped with a rare earth element at an amount of 2.0x10-5-1.0x10-2 parts by weight per 1 part by weight of magnesium oxide (MgO), a method of preparing the monocrystalline magnesium oxide by crystallizing it at about 2,800 DEG C., a protective layer formed of the same, and PDP including the protective layer.