Disclosed herein is a method for detecting at least one binding event of a specific binding pair-forming substance, comprising: providing a layer (2) of a first specific binding pair-forming substance on a substrate (3), the specific binding pair-forming substance having a density of 102-104 / [mu] m2 of the substrate (3). Providing a housing (4) of the first specific binding pair-forming substance layer (2) having a first
dielectric comprising a first solution having a first pH value and a first
ionic strength value, rinsing the housing (4) with a second
dielectric comprising a second solution having a second pH value and a second
ionic strength value for a first period of time, wherein at least the second pH value is different from the first pH value,-after the rinsing, recovering the first
dielectric having a first pH value and a first
ionic strength value in the housing (4),-feeding a solution of a second specific binding pair-forming substance into the housing (4) of the layer (2) of the first specific binding pair-forming substance, after the
recovery of the first dielectric in the shell (4), causing an interaction between the first specific binding pair-forming substance and a second specific binding pair-forming substance, and culturing the shell (4) for a second period of time, the interaction comprises a binding event when the first specific binding pair forming substance and the second specific binding pair forming substance provide said specific binding pair,-detecting an offset ([
Delta] [Theta]) of a parameter ([Theta]) representative of a
dielectric function of the layer (2) of the first specific binding pair forming substance after said incubation shell (4) lasts for a second period of time, the offset is defined as a change in a parameter ([theta]) representing a
dielectric function of a first specific binding pair forming a layer (2) of a substance from a first value occurring when the housing (4) comprises only the first dielectric after the
recovery of the first dielectric to a second value occurring after the incubation of the housing (4) for a second period of time, -comparing the offset ([
delta] [theta]) with a threshold value, whereby when the offset exceeds the threshold value, at least one binding event occurs between said first and second specific binding pair-forming substances.