The invention discloses a film magnetic characterization instrument under vacuum. The instrument includes an ultra-fast laser, beam splitters, a time delay line, reflectors, a polarization splitting prism, a frequency multiplier, two optical filters, at least one rectangular prism, an optical chopper, an optical focusing lens group, a multi-fiber sample rack, a hermetic vacuum cavity, a 1/2 slide, a beam splitter prism, a photodiode, a phase-locked amplifier, a magnet, a constant temperature apparatus, a translation stage, and an imaging apparatus. According to the invention, the instrument, by implementing in situ characterization in the preparation of films, increases measuring efficiency and precision. The instrument can achieve imaging effects by preparing a multi-fiber sample adjusting apparatus with as high as five fibers in combination with the translation stage for samples. Co-axial and vertical incidence of pump and detection beams simplifies light paths and reduces the difficulty in adjusting light paths. In addition, the instrument can enable samples to conduct magnetic characterization inside the vacuum cavity and prevents air or water vapor from polluting the samples.