Multilayer film dry chemical reagent tablet for detecting alanine aminotransferase

Abstract

The invention discloses a multilayer film dry chemical reagent tablet for detecting alanine aminotransferase, which comprises a lower support layer; a test hole is disposed at one end of the lower support layer; the lower support layer is coated with a multilayer film; the multilayer film comprises an euphotic layer, a reagent layer, an auxiliary layer and a diffusion layer which are orderly superimposed from up to down; one side of the euphotic layer of the multilayer film is bonded and fixed at the test hole of the lower support layer. The multilayer film dry tablet for detecting alanine aminotransferase of the invention can rapidly and accurately detect alanine aminotransferase in serum and blood plasma, and can increase the preservation time of the dry tablet.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical diagnostic reagents, in particular to a dry chemical reagent sheet with a multi-layer film for detecting alanine aminotransferase, which can be used for detecting transaminase in plasma and serum. Background technique [0002] Alanine aminotransferase (ALT), also known as alanine aminotransferase, mainly exists in the plasma of liver cells, and the intracellular concentration is 1000-3000 times higher than the serum concentration. As long as 1% of the liver tissue is destroyed, it can Doubling the serum concentration is recommended by the World Health Organization as the most sensitive detection index for liver function damage. Detection of alanine aminotransferase in blood is of great significance to the diagnosis and analysis of a series of viral hepatitis such as acute hepatitis B, chronic hepatitis, HBV carriers, severe hepatitis, liver cirrhosis, and liver cancer. [0003] At prese...

AI Technical Summary

Problems solved by technology

The production process of the multi-layer film dry film represented by Johnson & Johnson is complex and requires high material and equipment requirements; the dry film represented by Japan Kyoto Company has some defects, such as certain air bubbles and excess liquid are prone to be generated at the sample loading end, etc. Adding sample and measuring in the same test sample may cause interference

[0005] Some domestic companies have applied for dry chemical patents, Shanghai Kehua Bioengineering Co., Ltd. applied for patents 200720072185.2 and 200710044719.5, and Wuxi Shenrui Biological Products Co., Ltd. patented 200610161430.7, which described dry chemical test strips for the determination of alanine aminotransferase , the method used in these patents is to soak the prepared detection solution directly on the filter paper or fiber membrane, and then stick it on the plastic substrate for testing. This method is to soak the filter paper or cellulose membrane in the detection reagent , the amount of reagents adsorbed on filter paper or cellulose membrane cannot be determined, the repeatability cannot be fully guaranteed, and the accuracy and precision are lacking; in addition, this method uses chromogenic color detection at 610nm-650nm, while the detection of alanine amino The international standard for transferase uses the rate method to monitor the oxidation rate of NADH at 340nm. The reaction principle is as follows

Images