Clitopilus prunulus preserving method
A technology of oblique mushroom and sodium alginate, which is applied in the field of bioengineering, can solve the problems of liquid paraffin being toxic to bacterial cells, bacterial species death, etc., and achieve the effects of promoting production and research and prolonging preservation time.
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[0016] Example 1
[0017] The method of embedding and preserving Clitopilus prunulus purified water is as follows:
[0018] 1) Collect mycelium:
[0019] Inoculate Clitopilus prunulus, batch number TMI14-469 (strain provided by Ningxia Tairui Pharmaceutical Co., Ltd., seed room) in liquid PDA medium, 24~26℃, 220~240r / min, shake culture for 4d (At this time, Clitopilus prunulus is at the end of the logarithmic growth phase). Take 30mL of the above seed solution in a 50mL sterile centrifuge tube, centrifuge at 3500r / min for 10min. Discard the supernatant, and the precipitate is the mycelium, the mass is 8-10g.
[0020] 2) Preparation of embedding reagent:
[0021] Sodium alginate solution: sucrose 13.69% (w / v) + glycerol 18.4% (w / v) + sodium alginate 9% (w / v) to prepare 50 mL.
[0022] Calcium chloride solution: sucrose 13.69% (w / v) + glycerol 18.4% (w / v) + calcium chloride 1.11% (w / v), prepare 1 bottle, 400 mL.
[0023] Adjust the pH of the above two solutions to 6.8~7.0; autoclave at 1...
Example Embodiment
[0032] Example 2
[0033] The method of embedding and preserving Clitopilus prunulus purified water is as follows:
[0034] 1) Collect mycelium:
[0035] Inoculate Clitopilus prunulus, batch number TMI14-469 (strain provided by Ningxia Tairui Pharmaceutical Co., Ltd., seed room) in liquid PDA medium, 24~26℃, 220~240r / min, shake culture for 4d (At this time, Clitopilus prunulus is at the end of the logarithmic growth phase). Take 28 mL of the above seed liquid in a 50 mL sterile centrifuge tube, centrifuge at 3000 r / min for 10 min. Discard the supernatant, and the precipitate is the mycelium, the mass is 8-10g.
[0036] 2) Preparation of embedding reagent:
[0037] Sodium alginate solution: sucrose 13.69% (w / v) + glycerol 18.4% (w / v) + sodium alginate 11% (w / v) to prepare 50 mL.
[0038] Calcium chloride solution: sucrose 13.69% (w / v) + glycerol 18.4% (w / v) + calcium chloride 1.5% (w / v), prepared 2 bottles, 200 mL each.
[0039] Adjust the pH of the above two solutions to 6.8~7.0; autoc...
Example Embodiment
[0048] Example 3
[0049] The method of embedding and preserving Clitopilus prunulus purified water is as follows:
[0050] 1) Collect mycelium:
[0051] Inoculate Clitopilus prunulus, batch number TMI12-1096 (strains provided by Ningxia Tairui Pharmaceutical Co., Ltd., seed room) in liquid PDA medium, 24~26℃, 220~240r / min, shaking culture for 3d (At this time, Clitopilus prunulus is at the end of the logarithmic growth phase). Take 30 mL of the above seed liquid in a 50 mL sterile centrifuge tube, centrifuge at 3000 r / min for 15 min. The supernatant is discarded, and the precipitate is the mycelium, the mass is 9-10g.
[0052] 2) Preparation of embedding reagent:
[0053] Sodium alginate solution: sucrose 13.69% (w / v) + glycerol 18.4% (w / v) + sodium alginate 12% (w / v) to prepare 50 mL.
[0054] Calcium chloride solution: sucrose 13.69% (w / v) + glycerol 18.4% (w / v) + calcium chloride 3.0% (w / v), prepared 2 bottles, 250 mL each.
[0055] Adjust the pH of the above two solutions to 6.8~7...
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